Q8WVM8 (SCFD1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sec1 family domain-containing protein 1

Alternative name(s):
SLY1 homolog
Short name=Sly1p
Syntaxin-binding protein 1-like 2

Gene names

Name:	SCFD1
Synonyms:	C14orf163, KIAA0917, STXBP1L2
ORF Names:	FKSG23

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi By similarity.
Subunit structure	Binds STX5A. Interacts with the COG complex via COG4 By similarity.
Subcellular location	Cytoplasm By similarity. Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Golgi apparatus › Golgi stack membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the STXBP/unc-18/SEC1 family.
Sequence caution	The sequence AAD40381.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAD48586.1 differs from that shown. Reason: Frameshift at several positions. The sequence AAP97146.1 differs from that shown. Reason: Frameshift at position 5. The sequence BAA74940.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Endoplasmic reticulum Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	post-Golgi vesicle-mediated transport Inferred from sequence or structural similarity. Source: UniProtKB protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of ER to Golgi vesicle-mediated transport Inferred from sequence or structural similarity. Source: UniProtKB response to hypoxia Inferred from electronic annotation. Source: Compara response to toxin Inferred from expression pattern PubMed 15649705. Source: UniProtKB retrograde vesicle-mediated transport, Golgi to ER Inferred from sequence or structural similarity. Source: UniProtKB vesicle docking involved in exocytosis Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi transport complex Inferred from electronic annotation. Source: Compara Golgi-associated vesicle Inferred from sequence or structural similarity. Source: UniProtKB cis-Golgi network Inferred from direct assay PubMed 15649705. Source: UniProtKB endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	syntaxin binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7 Ref.8
	Chain	2 – 642	641	Sec1 family domain-containing protein 1		PRO_0000206287
Regions
	Compositional bias	2 – 13	12	Poly-Ala
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.8
	Modified residue	303	1	Phosphoserine Ref.11 Ref.12 Ref.13
Natural variations
	Natural variant	63	1	K → R. Ref.5 Ref.6 Corresponds to variant rs229150 [ dbSNP | Ensembl ].		VAR_019616
Experimental info
	Sequence conflict	42	1	V → I in AAG50273. Ref.3
	Sequence conflict	136 – 137	2	AA → EL in AAD40381. Ref.2
	Sequence conflict	201 – 206	6	FVTLGA → YGTRGD in AAD48586. Ref.2
	Sequence conflict	201 – 202	2	FV → YG in AAD40381. Ref.2
	Sequence conflict	201 – 202	2	FV → YG in AAP97146. Ref.4
	Sequence conflict	206	1	A → D in AAP97146. Ref.4
	Sequence conflict	453	1	K → N in AAD48586. Ref.2
	Sequence conflict	484 – 485	2	GC → EM in AAD40381. Ref.2
	Sequence conflict	485	1	Missing in AAD48586. Ref.2
	Sequence conflict	562 – 563	2	ET → KL in AAD40381. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8WVM8 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 0340FB9F05CD24CE	FASTA	642	72,380
	10 20 30 40 50 60 MAAAAAATAA AAASIRERQT VALKRMLNFN VPHIKNSTGE PVWKVLIYDR FGQDIISPLL 70 80 90 100 110 120 SVKELRDMGI TLHLLLHSDR DPIPDVPAVY FVMPTEENID RMCQDLRNQL YESYYLNFIS 130 140 150 160 170 180 AISRSKLEDI ANAALAASAV TQVAKVFDQY LNFITLEDDM FVLCNQNKEL VSYRAINRPD 190 200 210 220 230 240 ITDTEMETVM DTIVDSLFCF FVTLGAVPII RCSRGTAAEM VAVKLDKKLR ENLRDARNSL 250 260 270 280 290 300 FTGDTLGAGQ FSFQRPLLVL VDRNIDLATP LHHTWTYQAL VHDVLDFHLN RVNLEESSGV 310 320 330 340 350 360 ENSPAGARPK RKNKKSYDLT PVDKFWQKHK GSPFPEVAES VQQELESYRA QEDEVKRLKS 370 380 390 400 410 420 IMGLEGEDEG AISMLSDNTA KLTSAVSSLP ELLEKKRLID LHTNVATAVL EHIKARKLDV 430 440 450 460 470 480 YFEYEEKIMS KTTLDKSLLD IISDPDAGTP EDKMRLFLIY YISTQQAPSE ADLEQYKKAL 490 500 510 520 530 540 TDAGCNLNPL QYIKQWKAFT KMASAPASYG STTTKPMGLL SRVMNTGSQF VMEGVKNLVL 550 560 570 580 590 600 KQQNLPVTRI LDNLMEMKSN PETDDYRYFD PKMLRGNDSS VPRNKNPFQE AIVFVVGGGN 610 620 630 640 YIEYQNLVDY IKGKQGKHIL YGCSELFNAT QFIKQLSQLG QK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:355-364(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[2]	"Human vesicle transport-related protein gene." Song H., Peng Y., Dai M., Huang Q., Mao Y., Zhang Q., Mao M., Fu G., Luo M., Chen J., Hu R. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pituitary.
[3]	"Identification of FKSG23, a vesicle transport-related protein." Wang Y.-G., Gong L. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning of a novel human cDNA homology to R.norvegicus rsly1p mRNA." Dai F.Y., Yu L., Ding J.B., Lin W., Yang Y.M., Zhao S.Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-63. Tissue: Umbilical cord blood.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-63. Tissue: Lymph.
[7]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16. Tissue: Platelet.
[8]	Bienvenut W.V., Gao M., Leug H., Campbell A., Ozanne B.W. Submitted (JUL-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-16; 125-145; 382-395 AND 502-536, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Foreskin fibroblast and Prostatic carcinoma.
[9]	"Differential display of placental genes." Page N.M., Butlin D.J., Manyonda I., Bicknell A.B., Lowry P.J. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 572-642. Tissue: Placenta.
[10]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Embryonic kidney.
[11]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[12]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB020724 mRNA. Translation: BAA74940.2. Different initiation. AF092139 mRNA. Translation: AAD40381.1. Frameshift. AF110646 mRNA. Translation: AAD48586.1. Frameshift. AF319958 mRNA. Translation: AAG50273.1. AF086916 mRNA. Translation: AAP97146.1. Frameshift. AK290410 mRNA. Translation: BAF83099.1. BC017734 mRNA. Translation: AAH17734.1. AF067205 mRNA. Translation: AAC17907.1.
IPI	IPI00165261.
RefSeq	NP_057190.2. NM_016106.3.
UniGene	Hs.369168.
3D structure databases
ProteinModelPortal	Q8WVM8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8WVM8. 7 interactions.
MINT	MINT-1394008.
STRING	9606.ENSP00000390783.
PTM databases
PhosphoSite	Q8WVM8.
Polymorphism databases
DMDM	51316882.
Proteomic databases
PaxDb	Q8WVM8.
PRIDE	Q8WVM8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000458591; ENSP00000390783; ENSG00000092108.
GeneID	23256.
KEGG	hsa:23256.
UCSC	uc001wqm.1. human.
Organism-specific databases
CTD	23256.
GeneCards	GC14P031091.
HGNC	HGNC:20726. SCFD1.
HPA	HPA003579.
neXtProt	NX_Q8WVM8.
PharmGKB	PA134946073.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5158.
HOVERGEN	HBG061536.
OMA	TTKPMGL.
OrthoDB	EOG4BP1B8.
PhylomeDB	Q8WVM8.
Gene expression databases
ArrayExpress	Q8WVM8.
Bgee	Q8WVM8.
CleanEx	HS_SCFD1.
Genevestigator	Q8WVM8.
GermOnline	ENSG00000092108. Homo sapiens.
Family and domain databases
InterPro	IPR001619. Sec1-like. [Graphical view]
PANTHER	PTHR11679. PTHR11679. 1 hit.
Pfam	PF00995. Sec1. 1 hit. [Graphical view]
PIRSF	PIRSF005715. VPS45_Sec1. 1 hit.
SUPFAM	SSF56815. Sec1-like. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	SCFD1. human.
GenomeRNAi	23256.
NextBio	44982.

Entry information

Entry name

SCFD1_HUMAN

Accession

Primary (citable) accession number: Q8WVM8
Secondary accession number(s): A8K2Z5 , O60754, O94990, Q7Z529, Q9BZI3, Q9UNL3, Q9Y6A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 106 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.