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Q8WVD3

- RN138_HUMAN

UniProt

Q8WVD3 - RN138_HUMAN

Protein

E3 ubiquitin-protein ligase RNF138

Gene

RNF138

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri18 – 5841RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. protein kinase binding Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway
    2. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway, Wnt signaling pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase RNF138 (EC:6.3.2.-)
    Alternative name(s):
    Nemo-like kinase-associated RING finger protein
    Short name:
    NLK-associated RING finger protein
    Short name:
    hNARF
    RING finger protein 138
    Gene namesi
    Name:RNF138
    Synonyms:NARF
    ORF Names:HSD-4, HSD4
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:17765. RNF138.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProt

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134952071.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 245244E3 ubiquitin-protein ligase RNF138PRO_0000261607Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Post-translational modificationi

    Auto-ubiquitinated.

    Keywords - PTMi

    Acetylation, Ubl conjugation

    Proteomic databases

    MaxQBiQ8WVD3.
    PaxDbiQ8WVD3.
    PRIDEiQ8WVD3.

    PTM databases

    PhosphoSiteiQ8WVD3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8WVD3.
    BgeeiQ8WVD3.
    CleanExiHS_NARF.
    HS_RNF138.
    GenevestigatoriQ8WVD3.

    Organism-specific databases

    HPAiHPA041101.
    HPA041373.

    Interactioni

    Subunit structurei

    Interacts with NLK.1 Publication

    Protein-protein interaction databases

    BioGridi119545. 15 interactions.
    IntActiQ8WVD3. 12 interactions.
    MINTiMINT-1479614.
    STRINGi9606.ENSP00000261593.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8WVD3.
    SMRiQ8WVD3. Positions 18-107.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.By similarity

    Sequence similaritiesi

    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri18 – 5841RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG44750.
    HOGENOMiHOG000230946.
    HOVERGENiHBG074331.
    InParanoidiQ8WVD3.
    KOiK10668.
    OMAiEANFTRQ.
    OrthoDBiEOG73NG41.
    PhylomeDBiQ8WVD3.
    TreeFamiTF331012.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR008598. Di19_Zn_binding.
    IPR015880. Znf_C2H2-like.
    IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF00097. zf-C3HC4. 1 hit.
    PF05605. zf-Di19. 1 hit.
    [Graphical view]
    SMARTiSM00184. RING. 2 hits.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view]
    PROSITEiPS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8WVD3-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAEDLSAATS YTEDDFYCPV CQEVLKTPVR TTACQHVFCR KCFLTAMRES    50
    GAHCPLCRGN VTRRERACPE RALDLENIMR KFSGSCRCCA KQIKFYRMRH 100
    HYKSCKKYQD EYGVSSIIPN FQISQDSVGN SNRSETSTSD NTETYQENTS 150
    SSGHPTFKCP LCQESNFTRQ RLLDHCNSNH LFQIVPVTCP ICVSLPWGDP 200
    SQITRNFVSH LNQRHQFDYG EFVNLQLDEE TQYQTAVEES FQVNI 245
    Length:245
    Mass (Da):28,193
    Last modified:March 1, 2002 - v1
    Checksum:i807EE4500BE0919C
    GO
    Isoform 2 (identifier: Q8WVD3-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         38-131: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:151
    Mass (Da):17,262
    Checksum:i4FDCD6069E17B2BB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti118 – 1181I → V in AAD46623. 1 PublicationCurated
    Sequence conflicti215 – 2151H → R in AAD46623. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti81 – 811K → R.
    Corresponds to variant rs7229690 [ dbSNP | Ensembl ].
    VAR_052109

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei38 – 13194Missing in isoform 2. 1 PublicationVSP_021732Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF162680 mRNA. Translation: AAD46623.2.
    AK023579 mRNA. Translation: BAB14614.1.
    AK315761 mRNA. Translation: BAG38114.1.
    BT006878 mRNA. Translation: AAP35524.1.
    CR457150 mRNA. Translation: CAG33431.1.
    CH471088 Genomic DNA. Translation: EAX01280.1.
    BC018107 mRNA. Translation: AAH18107.1.
    AL133557 mRNA. Translation: CAB63712.1.
    CCDSiCCDS11903.1. [Q8WVD3-1]
    CCDS11904.1. [Q8WVD3-2]
    PIRiT43439.
    RefSeqiNP_001178253.2. NM_001191324.1. [Q8WVD3-1]
    NP_057355.2. NM_016271.4. [Q8WVD3-1]
    NP_937761.1. NM_198128.2. [Q8WVD3-2]
    XP_005258343.1. XM_005258286.1. [Q8WVD3-2]
    UniGeneiHs.302408.

    Genome annotation databases

    EnsembliENST00000257190; ENSP00000257190; ENSG00000134758. [Q8WVD3-2]
    ENST00000261593; ENSP00000261593; ENSG00000134758. [Q8WVD3-1]
    GeneIDi51444.
    KEGGihsa:51444.
    UCSCiuc002kxg.3. human. [Q8WVD3-1]
    uc002kxh.3. human. [Q8WVD3-2]

    Polymorphism databases

    DMDMi74762632.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF162680 mRNA. Translation: AAD46623.2 .
    AK023579 mRNA. Translation: BAB14614.1 .
    AK315761 mRNA. Translation: BAG38114.1 .
    BT006878 mRNA. Translation: AAP35524.1 .
    CR457150 mRNA. Translation: CAG33431.1 .
    CH471088 Genomic DNA. Translation: EAX01280.1 .
    BC018107 mRNA. Translation: AAH18107.1 .
    AL133557 mRNA. Translation: CAB63712.1 .
    CCDSi CCDS11903.1. [Q8WVD3-1 ]
    CCDS11904.1. [Q8WVD3-2 ]
    PIRi T43439.
    RefSeqi NP_001178253.2. NM_001191324.1. [Q8WVD3-1 ]
    NP_057355.2. NM_016271.4. [Q8WVD3-1 ]
    NP_937761.1. NM_198128.2. [Q8WVD3-2 ]
    XP_005258343.1. XM_005258286.1. [Q8WVD3-2 ]
    UniGenei Hs.302408.

    3D structure databases

    ProteinModelPortali Q8WVD3.
    SMRi Q8WVD3. Positions 18-107.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119545. 15 interactions.
    IntActi Q8WVD3. 12 interactions.
    MINTi MINT-1479614.
    STRINGi 9606.ENSP00000261593.

    PTM databases

    PhosphoSitei Q8WVD3.

    Polymorphism databases

    DMDMi 74762632.

    Proteomic databases

    MaxQBi Q8WVD3.
    PaxDbi Q8WVD3.
    PRIDEi Q8WVD3.

    Protocols and materials databases

    DNASUi 51444.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000257190 ; ENSP00000257190 ; ENSG00000134758 . [Q8WVD3-2 ]
    ENST00000261593 ; ENSP00000261593 ; ENSG00000134758 . [Q8WVD3-1 ]
    GeneIDi 51444.
    KEGGi hsa:51444.
    UCSCi uc002kxg.3. human. [Q8WVD3-1 ]
    uc002kxh.3. human. [Q8WVD3-2 ]

    Organism-specific databases

    CTDi 51444.
    GeneCardsi GC18P029671.
    HGNCi HGNC:17765. RNF138.
    HPAi HPA041101.
    HPA041373.
    neXtProti NX_Q8WVD3.
    PharmGKBi PA134952071.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG44750.
    HOGENOMi HOG000230946.
    HOVERGENi HBG074331.
    InParanoidi Q8WVD3.
    KOi K10668.
    OMAi EANFTRQ.
    OrthoDBi EOG73NG41.
    PhylomeDBi Q8WVD3.
    TreeFami TF331012.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_75842. Antigen processing: Ubiquitination & Proteasome degradation.

    Miscellaneous databases

    GenomeRNAii 51444.
    NextBioi 55035.
    PROi Q8WVD3.

    Gene expression databases

    ArrayExpressi Q8WVD3.
    Bgeei Q8WVD3.
    CleanExi HS_NARF.
    HS_RNF138.
    Genevestigatori Q8WVD3.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR008598. Di19_Zn_binding.
    IPR015880. Znf_C2H2-like.
    IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF00097. zf-C3HC4. 1 hit.
    PF05605. zf-Di19. 1 hit.
    [Graphical view ]
    SMARTi SM00184. RING. 2 hits.
    SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    PROSITEi PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new spermatogenesis-related gene."
      Wang L., Miao S., Yang J., Zhang X., Li M.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Testis.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Placenta and Trachea.
    3. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Uterus.
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 63-245 (ISOFORM 1).
      Tissue: Testis.
    8. "NARF, an nemo-like kinase (NLK)-associated ring finger protein regulates the ubiquitylation and degradation of T cell factor/lymphoid enhancer factor (TCF/LEF)."
      Yamada M., Ohnishi J., Ohkawara B., Iemura S., Satoh K., Hyodo-Miura J., Kawachi K., Natsume T., Shibuya H.
      J. Biol. Chem. 281:20749-20760(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NLK.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRN138_HUMAN
    AccessioniPrimary (citable) accession number: Q8WVD3
    Secondary accession number(s): B2RE17
    , Q9H8K2, Q9UF87, Q9UKI6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2006
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3