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Q8WV41

- SNX33_HUMAN

UniProt

Q8WV41 - SNX33_HUMAN

Protein

Sorting nexin-33

Gene

SNX33

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Plays a role in the reorganization of the cytoskeleton, endocytosis and cellular vesicle trafficking via its interactions with membranes, WASL, DNM1 and DNM2. Acts both during interphase and at the end of mitotic cell divisions. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Modulates endocytosis of cell-surface proteins, such as APP and PRNP; this then modulates the secretion of APP and PRNP peptides. Promotes membrane tubulation (in vitro). May promote the formation of macropinosomes.6 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. phosphatidylinositol binding Source: RefGenome
    3. protein binding Source: IntAct

    GO - Biological processi

    1. cleavage furrow formation Source: UniProtKB
    2. endocytosis Source: UniProtKB
    3. endosomal transport Source: UniProtKB
    4. endosome organization Source: UniProtKB
    5. intracellular protein transport Source: RefGenome
    6. macropinocytosis Source: UniProtKB
    7. membrane tubulation Source: UniProtKB
    8. mitotic cytokinesis Source: UniProtKB
    9. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Endocytosis, Mitosis, Protein transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sorting nexin-33
    Alternative name(s):
    SH3 and PX domain-containing protein 3
    Gene namesi
    Name:SNX33
    Synonyms:SH3PX3, SH3PXD3C, SNX30
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:28468. SNX33.

    Subcellular locationi

    Cytoplasmcytosol. Membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Primarily cytosolic, but a minor proportion is membrane-bound (PubMed:18353773). Not associated with membranes (PubMed:21048941).2 Publications

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: UniProtKB
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. cytosol Source: UniProtKB-SubCell
    4. endosome Source: RefGenome
    5. extrinsic component of membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA162404345.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 574574Sorting nexin-33PRO_0000311948Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei77 – 771Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylated.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8WV41.
    PaxDbiQ8WV41.
    PRIDEiQ8WV41.

    PTM databases

    PhosphoSiteiQ8WV41.

    Expressioni

    Tissue specificityi

    Detected in heart and pancreas.1 Publication

    Gene expression databases

    ArrayExpressiQ8WV41.
    BgeeiQ8WV41.
    CleanExiHS_SNX30.
    HS_SNX33.
    GenevestigatoriQ8WV41.

    Organism-specific databases

    HPAiHPA040988.

    Interactioni

    Subunit structurei

    Homodimer (via BAR domain). Interacts with ADAM15. Interacts with FASLG. Interacts (via SH3 domain) with DNM1 and DNM2. Interacts with WASL.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-2481535,EBI-2481535
    ADAM15Q134442EBI-2481535,EBI-77818
    FASLGP480232EBI-2481535,EBI-495538
    SNX9Q9Y5X12EBI-2481535,EBI-77848
    WASP427683EBI-2481535,EBI-346375

    Protein-protein interaction databases

    BioGridi129214. 3 interactions.
    IntActiQ8WV41. 5 interactions.
    MINTiMINT-2792452.
    STRINGi9606.ENSP00000311427.

    Structurei

    Secondary structure

    1
    574
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi213 – 2153
    Beta strandi220 – 2234
    Beta strandi232 – 2354
    Beta strandi252 – 2565
    Beta strandi259 – 2613
    Beta strandi263 – 2653
    Helixi267 – 28014
    Beta strandi282 – 2843
    Turni302 – 3043
    Helixi305 – 31814
    Helixi323 – 3253
    Helixi327 – 3337
    Helixi339 – 34911
    Helixi355 – 3606
    Helixi371 – 40737
    Helixi409 – 42719
    Helixi432 – 4343
    Helixi437 – 45923
    Helixi460 – 4623
    Helixi465 – 50440
    Helixi510 – 56859
    Helixi569 – 5713

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4AKVX-ray2.65A/B212-574[»]
    ProteinModelPortaliQ8WV41.
    SMRiQ8WV41. Positions 1-100, 212-573.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 6161SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini230 – 340111PXPROSITE-ProRule annotationAdd
    BLAST
    Domaini371 – 574204BARAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi112 – 12211Poly-AspAdd
    BLAST

    Domaini

    The PX and BAR domains mediate association with membranes and are required for membrane tubulation.1 Publication

    Sequence similaritiesi

    Belongs to the sorting nexin family.Curated
    Contains 1 BAR domain.Curated
    Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiCOG5391.
    HOGENOMiHOG000261633.
    HOVERGENiHBG009996.
    InParanoidiQ8WV41.
    KOiK17923.
    OMAiQGSFEDD.
    OrthoDBiEOG7ZKS9P.
    PhylomeDBiQ8WV41.
    TreeFamiTF314082.

    Family and domain databases

    Gene3Di3.30.1520.10. 1 hit.
    InterProiIPR001683. Phox.
    IPR001452. SH3_domain.
    IPR028642. SNX33.
    IPR014536. Snx9_subfam.
    IPR019497. Sorting_nexin_WASP-bd-dom.
    [Graphical view]
    PANTHERiPTHR10555:SF121. PTHR10555:SF121. 1 hit.
    PfamiPF10456. BAR_3_WASP_bdg. 1 hit.
    PF00787. PX. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PIRSFiPIRSF027744. Snx9. 1 hit.
    SMARTiSM00312. PX. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF64268. SSF64268. 1 hit.
    PROSITEiPS50195. PX. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8WV41-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALKGRALYD FHSENKEEIS IQQDEDLVIF SETSLDGWLQ GQNSRGETGL    50
    FPASYVEIVR SGISTNHADY SSSPAGSPGA QVSLYNSPSV ASPARSGGGS 100
    GFLSNQGSFE EDDDDDWDDW DDGCTVVEEP RAGGLGTNGH PPLNLSYPGA 150
    YPSQHMAFRP KPPLERQDSL ASAKRGSVVG RNLNRFSCFV RSGVEAFILG 200
    DVPMMAKIAE TYSIEMGPRG PQWKANPHPF ACSVEDPTKQ TKFKGIKSYI 250
    SYKLTPTHAA SPVYRRYKHF DWLYNRLLHK FTVISVPHLP EKQATGRFEE 300
    DFIEKRKRRL ILWMDHMTSH PVLSQYEGFQ HFLSCLDDKQ WKMGKRRAEK 350
    DEMVGASFLL TFQIPTEHQD LQDVEDRVDT FKAFSKKMDD SVLQLSTVAS 400
    ELVRKHVGGF RKEFQKLGSA FQAISHSFQM DPPFCSEALN SAISHTGRTY 450
    EAIGEMFAEQ PKNDLFQMLD TLSLYQGLLS NFPDIIHLQK GAFAKVKESQ 500
    RMSDEGRMVQ DEADGIRRRC RVVGFALQAE MNHFHQRREL DFKHMMQNYL 550
    RQQILFYQRV GQQLEKTLRM YDNL 574
    Length:574
    Mass (Da):65,265
    Last modified:March 1, 2002 - v1
    Checksum:i7CE51C0F35DDBC3C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF219141 mRNA. Translation: ABN09670.1.
    EF653821 mRNA. Translation: ABV26009.1.
    AL833039 mRNA. Translation: CAH56299.1.
    CH471136 Genomic DNA. Translation: EAW99243.1.
    BC018775 mRNA. Translation: AAH18775.1.
    CCDSiCCDS10283.1.
    RefSeqiNP_695003.1. NM_153271.1.
    UniGeneiHs.8705.

    Genome annotation databases

    EnsembliENST00000308527; ENSP00000311427; ENSG00000173548.
    GeneIDi257364.
    KEGGihsa:257364.
    UCSCiuc002bau.3. human.

    Polymorphism databases

    DMDMi74751538.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF219141 mRNA. Translation: ABN09670.1 .
    EF653821 mRNA. Translation: ABV26009.1 .
    AL833039 mRNA. Translation: CAH56299.1 .
    CH471136 Genomic DNA. Translation: EAW99243.1 .
    BC018775 mRNA. Translation: AAH18775.1 .
    CCDSi CCDS10283.1.
    RefSeqi NP_695003.1. NM_153271.1.
    UniGenei Hs.8705.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4AKV X-ray 2.65 A/B 212-574 [» ]
    ProteinModelPortali Q8WV41.
    SMRi Q8WV41. Positions 1-100, 212-573.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 129214. 3 interactions.
    IntActi Q8WV41. 5 interactions.
    MINTi MINT-2792452.
    STRINGi 9606.ENSP00000311427.

    PTM databases

    PhosphoSitei Q8WV41.

    Polymorphism databases

    DMDMi 74751538.

    Proteomic databases

    MaxQBi Q8WV41.
    PaxDbi Q8WV41.
    PRIDEi Q8WV41.

    Protocols and materials databases

    DNASUi 257364.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000308527 ; ENSP00000311427 ; ENSG00000173548 .
    GeneIDi 257364.
    KEGGi hsa:257364.
    UCSCi uc002bau.3. human.

    Organism-specific databases

    CTDi 257364.
    GeneCardsi GC15P075940.
    H-InvDB HIX0012446.
    HGNCi HGNC:28468. SNX33.
    HPAi HPA040988.
    neXtProti NX_Q8WV41.
    PharmGKBi PA162404345.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5391.
    HOGENOMi HOG000261633.
    HOVERGENi HBG009996.
    InParanoidi Q8WV41.
    KOi K17923.
    OMAi QGSFEDD.
    OrthoDBi EOG7ZKS9P.
    PhylomeDBi Q8WV41.
    TreeFami TF314082.

    Miscellaneous databases

    GenomeRNAii 257364.
    NextBioi 92998.
    PROi Q8WV41.

    Gene expression databases

    ArrayExpressi Q8WV41.
    Bgeei Q8WV41.
    CleanExi HS_SNX30.
    HS_SNX33.
    Genevestigatori Q8WV41.

    Family and domain databases

    Gene3Di 3.30.1520.10. 1 hit.
    InterProi IPR001683. Phox.
    IPR001452. SH3_domain.
    IPR028642. SNX33.
    IPR014536. Snx9_subfam.
    IPR019497. Sorting_nexin_WASP-bd-dom.
    [Graphical view ]
    PANTHERi PTHR10555:SF121. PTHR10555:SF121. 1 hit.
    Pfami PF10456. BAR_3_WASP_bdg. 1 hit.
    PF00787. PX. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF027744. Snx9. 1 hit.
    SMARTi SM00312. PX. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF64268. SSF64268. 1 hit.
    PROSITEi PS50195. PX. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome."
      Kaerkkaeinen S., Hiipakka M., Wang J.-H., Kleino I., Vaehae-Jaakkola M., Renkema G.H., Liss M., Wagner R., Saksela K.
      EMBO Rep. 7:186-191(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH ADAM15.
    2. "A novel sorting nexin modulates endocytic trafficking and alpha-secretase cleavage of the amyloid precursor protein."
      Schobel S., Neumann S., Hertweck M., Dislich B., Kuhn P.H., Kremmer E., Seed B., Baumeister R., Haass C., Lichtenthaler S.F.
      J. Biol. Chem. 283:14257-14268(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH DNM1 AND DNM2, SUBCELLULAR LOCATION, PHOSPHORYLATION, TISSUE SPECIFICITY.
      Tissue: Brain.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Stomach.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skin.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    7. "The novel sorting nexin SNX33 interferes with cellular PrP formation by modulation of PrP shedding."
      Heiseke A., Schobel S., Lichtenthaler S.F., Vorberg I., Groschup M.H., Kretzschmar H., Schatzl H.M., Nunziante M.
      Traffic 9:1116-1129(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening."
      Voss M., Lettau M., Janssen O.
      BMC Immunol. 10:53-53(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FASLG.
    9. "Sorting nexin 33 induces mammalian cell micronucleated phenotype and actin polymerization by interacting with Wiskott-Aldrich syndrome protein."
      Zhang J., Zhang X., Guo Y., Xu L., Pei D.
      J. Biol. Chem. 284:21659-21669(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH WASL.
    10. "Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins."
      Kleino I., Ortiz R.M., Yritys M., Huovila A.P., Saksela K.
      J. Cell. Biochem. 108:877-885(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ADAM15.
    11. "The SNX-PX-BAR family in macropinocytosis: the regulation of macropinosome formation by SNX-PX-BAR proteins."
      Wang J.T., Kerr M.C., Karunaratne S., Jeanes A., Yap A.S., Teasdale R.D.
      PLoS ONE 5:E13763-E13763(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    12. "Specific amino acids in the BAR domain allow homodimerization and prevent heterodimerization of sorting nexin 33."
      Dislich B., Than M.E., Lichtenthaler S.F.
      Biochem. J. 433:75-83(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DOMAIN, SUBCELLULAR LOCATION, SUBUNIT.
    13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "SNX9, SNX18 and SNX33 are required for progression through and completion of mitosis."
      Ma M.P., Chircop M.
      J. Cell Sci. 125:4372-4382(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    15. "Structure of human sorting nexin 33."
      Structural genomics consortium (SGC)
      Submitted (DEC-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 212-574.

    Entry informationi

    Entry nameiSNX33_HUMAN
    AccessioniPrimary (citable) accession number: Q8WV41
    Secondary accession number(s): B1NM17
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3