Reviewed, UniProtKB/Swiss-Prot Q8WV24 (PHLA1_HUMAN)

Names and origin

Protein names

Recommended name:
    Pleckstrin homology-like domain family A member 1
Alternative name(s):
    T-cell death-associated gene 51 protein
    Apoptosis-associated nuclear protein
    Proline- and histidine-rich protein
    Proline- and glutamine-rich protein
    PQ-rich protein

Gene names

Name:	PHLDA1
Synonyms:	PHRIP, TDAG51

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-apoptotic effects of IGF1. May be involved in translational regulation. Ref.5 Ref.6
Subunit structure	Interacts with RPL14, EIF3S7 and PABPC4. Ref.5
Subcellular location	Cytoplasm. Cytoplasmic vesicle membrane. Nucleus › nucleolus. Note: Colocalizes with intracellular vesicles. Ref.5 Ref.6
Tissue specificity	Widely expressed with highest levels in pancreas. Strongly expressed by benign melanocytic nevi, and progressively reduced expressed in primary and metastatic melanomas (at protein level). Ref.6 Ref.4
Induction	Induced by homocysteine and other ER stress-inducing reagents. Induced by phorbolester (TPA)/ionomycin, and stimulation of the T-cell receptor (TCR) complex in T-cells. Ref.6
Sequence similarities	Contains 1 PH domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Cytoplasmic vesicle Membrane Nucleus
Domain	Repeat
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	apoptosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF3D	O15371	1	EBI-738731,EBI-353818
PABPC4	Q13310	1	EBI-738731,EBI-372844
RPL14	P50914	1	EBI-738731,EBI-356746

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Pleckstrin homology-like domain family A member 1		PRO_0000053897
Regions
	Domain	151 – 283	133	PH
	Region	311 – 346	36	15 X 2 AA repeats of P-Q
	Region	352 – 389	38	14 X 2 AA repeats of P-H
	Compositional bias	187 – 204	18	Poly-Gln
Experimental info
	Sequence conflict	204	1	Missing in AAF64165. Ref.1
	Sequence conflict	204	1	Missing in CAA90576. Ref.2
	Sequence conflict	204	1	Missing Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8WV24-1 [UniParc]. Last modified May 5, 2009. Version 4. Checksum: 1E01E7F75116A517	FASTA	401	45,016
	10 20 30 40 50 60 MRRAPAAERL LELGFPPRCG RQEPPFPLGV TRGWGRWPIQ KRREGARPVP FSERSQEDGR 70 80 90 100 110 120 GPAARSSGTL WRIRTRLSLC RDPEPPPPLC LLRVSLLCAL RAGGRGSRWG EDGARLLLLP 130 140 150 160 170 180 PARAAGNGEA EPSGGPSYAG RMLESSGCKA LKEGVLEKRS DGLLQLWKKK CCILTEEGLL 190 200 210 220 230 240 LIPPKQLQHQ QQQQQQQQQQ QQQQPGQGPA EPSQPSGPAV ASLEPPVKLK ELHFSNMKTV 250 260 270 280 290 300 DCVERKGKYM YFTVVMAEGK EIDFRCPQDQ GWNAEITLQM VQYKNRQAIL AVKSTRQKQQ 310 320 330 340 350 360 HLVQQQPPSQ PQPQPQLQPQ PQPQPQPQPQ PQSQPQPQPQ PKPQPQQLHP YPHPHPHPHS 370 380 390 400 HPHSHPHPHP HPHPHQIPHP HPQPHSQPHG HRLLRSTSNS A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Assignment of the human PHLDA1 gene to chromosome 12q15 by radiation hybrid mapping." Kuske M.D., Johnson J.P. Cytogenet. Cell Genet. 89:1-1(2000) [PubMed: 10894922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A cDNA, which predicts a protein with PQ-rich repeats, isolated from a phage library of human fetal liver tissue." Wagner F.F., Flegel W.A. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 53-401. Tissue: Brain and Uterus.
[4]	"Identification of the human PHLDA1/TDAG51 gene: down-regulation in metastatic melanoma contributes to apoptosis resistance and growth deregulation." Neef R., Kuske M.A., Proels E., Johnson J.P. Cancer Res. 62:5920-5929(2002) [PubMed: 12384558] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 350-401, TISSUE SPECIFICITY.
[5]	"Inhibition of protein synthesis by the T cell receptor-inducible human TDAG51 gene product." Hinz T., Flindt S., Marx A., Janssen O., Kabelitz D. Cell. Signal. 13:345-352(2001) [PubMed: 11369516] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RPL14; EIF3S7 AND PABPC4.
[6]	"TDAG51 is induced by homocysteine, promotes detachment-mediated programmed cell death, and contributes to the cevelopment of atherosclerosis in hyperhomocysteinemia." Hossain G.S., van Thienen J.V., Werstuck G.H., Zhou J., Sood S.K., Dickhout J.G., de Koning A.B., Tang D., Wu D., Falk E., Poddar R., Jacobsen D.W., Zhang K., Kaufman R.J., Austin R.C. J. Biol. Chem. 278:30317-30327(2003) [PubMed: 12738777] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF239986 Genomic DNA. Translation: AAF64165.1. Z50194 mRNA. Translation: CAA90576.1. BC018929 mRNA. Translation: AAH18929.3. Different initiation. BC110820 mRNA. Translation: AAI10821.1. Different initiation. BC126425 mRNA. Translation: AAI26426.2. Different initiation. AF220656 mRNA. Translation: AAF36387.1.
IPI	IPI00025463.
PIR	S58222.
RefSeq	NP_031376.3.
UniGene	Hs.602085
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8WV24. 6 interactions.
Proteomic databases
PRIDE	Q8WV24.
Genome annotation databases
Ensembl	ENSG00000139289. Homo sapiens. [Contig view]
GeneID	22822.
Organism-specific databases
GeneCards	GC12M074707.
H-InvDB	HIX0010830.
HGNC	HGNC:8933. PHLDA1.
MIM	605335. gene.
PharmGKB	PA33274.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	Q8WV24.
HOVERGEN	Q8WV24.
Gene expression databases
ArrayExpress	Q8WV24.
Bgee	Q8WV24.
CleanEx	HS_PHLDA1.
GermOnline	ENSG00000139289. Homo sapiens.
Family and domain databases
InterPro	IPR001849. Pleckstrin_homology. [Graphical view]
SMART	SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PHLA1_HUMAN

Accession

Primary (citable) accession number: Q8WV24
Secondary accession number(s): A1A4G9 , Q15184, Q2TAN2, Q9NZ17

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	May 5, 2009
Last modified:	July 7, 2009
This is version 50 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)