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Protein

DDB1- and CUL4-associated factor 4

Gene

DCAF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciZFISH:ENSG00000119599-MONOMER.
SignaLinkiQ8WV16.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
DDB1- and CUL4-associated factor 4
Alternative name(s):
WD repeat-containing protein 21A
Gene namesi
Name:DCAF4
Synonyms:WDR21, WDR21A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:20229. DCAF4.

Subcellular locationi

GO - Cellular componenti

  • Cul4-RING E3 ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi26094.
OpenTargetsiENSG00000119599.
PharmGKBiPA165478762.

Polymorphism and mutation databases

BioMutaiDCAF4.
DMDMi116242844.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000513681 – 495DDB1- and CUL4-associated factor 4Add BLAST495

Proteomic databases

MaxQBiQ8WV16.
PaxDbiQ8WV16.
PeptideAtlasiQ8WV16.
PRIDEiQ8WV16.

PTM databases

iPTMnetiQ8WV16.
PhosphoSitePlusiQ8WV16.

Expressioni

Gene expression databases

BgeeiENSG00000119599.
CleanExiHS_WDR21A.
ExpressionAtlasiQ8WV16. baseline and differential.
GenevisibleiQ8WV16. HS.

Organism-specific databases

HPAiHPA047276.

Interactioni

Subunit structurei

Interacts with DDB1 and CUL4A.1 Publication

Protein-protein interaction databases

BioGridi117545. 28 interactors.
DIPiDIP-48761N.
IntActiQ8WV16. 6 interactors.
STRINGi9606.ENSP00000351147.

Structurei

Secondary structure

1495
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi126 – 134Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I8CX-ray2.80B124-136[»]
ProteinModelPortaliQ8WV16.
SMRiQ8WV16.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WV16.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati368 – 407WD 1Add BLAST40
Repeati410 – 451WD 2Add BLAST42

Sequence similaritiesi

Contains 2 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2695. Eukaryota.
ENOG410XQIU. LUCA.
GeneTreeiENSGT00390000009546.
HOGENOMiHOG000065756.
HOVERGENiHBG054724.
InParanoidiQ8WV16.
KOiK11799.
OMAiYLMASDM.
OrthoDBiEOG091G06QG.
PhylomeDBiQ8WV16.
TreeFamiTF332050.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WV16-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNKSRWQSRR RHGRRSHQQN PWFRLRDSED RSDSRAAQPA HDSGHGDDES
60 70 80 90 100
PSTSSGTAGT SSVPELPGFY FDPEKKRYFR LLPGHNNCNP LTKESIRQKE
110 120 130 140 150
MESKRLRLLQ EEDRRKKIAR MGFNASSMLR KSQLGFLNVT NYCHLAHELR
160 170 180 190 200
LSCMERKKVQ IRSMDPSALA SDRFNLILAD TNSDRLFTVN DVKVGGSKYG
210 220 230 240 250
IINLQSLKTP TLKVFMHENL YFTNRKVNSV CWASLNHLDS HILLCLMGLA
260 270 280 290 300
ETPGCATLLP ASLFVNSHPG IDRPGMLCSF RIPGAWSCAW SLNIQANNCF
310 320 330 340 350
STGLSRRVLL TNVVTGHRQS FGTNSDVLAQ QFALMAPLLF NGCRSGEIFA
360 370 380 390 400
IDLRCGNQGK GWKATRLFHD SAVTSVRILQ DEQYLMASDM AGKIKLWDLR
410 420 430 440 450
TTKCVRQYEG HVNEYAYLPL HVHEEEGILV AVGQDCYTRI WSLHDARLLR
460 470 480 490
TIPSPYPASK ADIPSVAFSS RLGGSRGAPG LLMAVGQDLY CYSYS
Length:495
Mass (Da):55,694
Last modified:October 17, 2006 - v3
Checksum:iF104B7F1FC6B5C33
GO
Isoform 2 (identifier: Q8WV16-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     432-433: VG → GT
     434-495: Missing.

Note: No experimental confirmation available.
Show »
Length:433
Mass (Da):48,933
Checksum:i109E83E34CC003B2
GO
Isoform 3 (identifier: Q8WV16-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-178: Missing.
     269-269: P → PA

Note: No experimental confirmation available.
Show »
Length:435
Mass (Da):48,798
Checksum:iB1C5BAC49BDD9B60
GO
Isoform 4 (identifier: Q8WV16-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Show »
Length:395
Mass (Da):44,161
Checksum:i6BC9F63E4A6D85D3
GO
Isoform 5 (identifier: Q8WV16-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     269-269: P → PA
     329-335: Missing.

Note: No experimental confirmation available.
Show »
Length:489
Mass (Da):54,975
Checksum:i362DF3B15C3D7656
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4S → G in CAB45748 (PubMed:17974005).Curated1
Sequence conflicti35R → Q in BAB55337 (PubMed:14702039).Curated1
Sequence conflicti45H → Y in BAB55337 (PubMed:14702039).Curated1
Sequence conflicti65E → G in BAB55337 (PubMed:14702039).Curated1

Polymorphismi

An intronic G-to-A transition (rs2535913) has been associated with leukocyte telomere length. The minor A allele is associated with shorter telomeres and lower expression in lymphoblastoid cells and in sun-exposed skin (PubMed:25624462).1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02790822W → C.Corresponds to variant rs2302588dbSNPEnsembl.1
Natural variantiVAR_027909193K → T.2 PublicationsCorresponds to variant rs17856582dbSNPEnsembl.1
Natural variantiVAR_027910266N → S.Corresponds to variant rs7155812dbSNPEnsembl.1
Natural variantiVAR_027911334L → F.2 PublicationsCorresponds to variant rs17856583dbSNPEnsembl.1
Natural variantiVAR_027912345S → C.Corresponds to variant rs3815460dbSNPEnsembl.1
Natural variantiVAR_027913439R → I.1 PublicationCorresponds to variant rs17856584dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0454271 – 100Missing in isoform 4. 1 PublicationAdd BLAST100
Alternative sequenceiVSP_047171118 – 178Missing in isoform 3. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_047172269P → PA in isoform 3 and isoform 5. 1 Publication1
Alternative sequenceiVSP_047173329 – 335Missing in isoform 5. 1 Publication7
Alternative sequenceiVSP_020986432 – 433VG → GT in isoform 2. 1 Publication2
Alternative sequenceiVSP_020987434 – 495Missing in isoform 2. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX247962 mRNA. Translation: CAD62301.1.
AK027745 mRNA. Translation: BAB55337.1.
AK300786 mRNA. Translation: BAG62448.1.
AC007160 Genomic DNA. No translation available.
AL442663 Genomic DNA. No translation available.
BC018979 mRNA. Translation: AAH18979.1.
BC035901 mRNA. Translation: AAH35901.1.
AL080157 mRNA. Translation: CAB45748.1.
CCDSiCCDS41968.2. [Q8WV16-3]
CCDS55926.1. [Q8WV16-5]
CCDS9809.1. [Q8WV16-1]
CCDS9810.1. [Q8WV16-4]
PIRiT12541.
RefSeqiNP_001156980.1. NM_001163508.1. [Q8WV16-5]
NP_001156981.1. NM_001163509.1.
NP_056419.2. NM_015604.3. [Q8WV16-1]
NP_851937.1. NM_181340.2. [Q8WV16-4]
NP_851938.2. NM_181341.2. [Q8WV16-3]
XP_016876696.1. XM_017021207.1. [Q8WV16-1]
XP_016876697.1. XM_017021208.1. [Q8WV16-1]
UniGeneiHs.331491.

Genome annotation databases

EnsembliENST00000358377; ENSP00000351147; ENSG00000119599. [Q8WV16-1]
ENST00000394234; ENSP00000377781; ENSG00000119599. [Q8WV16-4]
ENST00000509153; ENSP00000426178; ENSG00000119599. [Q8WV16-3]
ENST00000555042; ENSP00000452131; ENSG00000119599. [Q8WV16-5]
GeneIDi26094.
KEGGihsa:26094.
UCSCiuc001xng.4. human. [Q8WV16-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX247962 mRNA. Translation: CAD62301.1.
AK027745 mRNA. Translation: BAB55337.1.
AK300786 mRNA. Translation: BAG62448.1.
AC007160 Genomic DNA. No translation available.
AL442663 Genomic DNA. No translation available.
BC018979 mRNA. Translation: AAH18979.1.
BC035901 mRNA. Translation: AAH35901.1.
AL080157 mRNA. Translation: CAB45748.1.
CCDSiCCDS41968.2. [Q8WV16-3]
CCDS55926.1. [Q8WV16-5]
CCDS9809.1. [Q8WV16-1]
CCDS9810.1. [Q8WV16-4]
PIRiT12541.
RefSeqiNP_001156980.1. NM_001163508.1. [Q8WV16-5]
NP_001156981.1. NM_001163509.1.
NP_056419.2. NM_015604.3. [Q8WV16-1]
NP_851937.1. NM_181340.2. [Q8WV16-4]
NP_851938.2. NM_181341.2. [Q8WV16-3]
XP_016876696.1. XM_017021207.1. [Q8WV16-1]
XP_016876697.1. XM_017021208.1. [Q8WV16-1]
UniGeneiHs.331491.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I8CX-ray2.80B124-136[»]
ProteinModelPortaliQ8WV16.
SMRiQ8WV16.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117545. 28 interactors.
DIPiDIP-48761N.
IntActiQ8WV16. 6 interactors.
STRINGi9606.ENSP00000351147.

PTM databases

iPTMnetiQ8WV16.
PhosphoSitePlusiQ8WV16.

Polymorphism and mutation databases

BioMutaiDCAF4.
DMDMi116242844.

Proteomic databases

MaxQBiQ8WV16.
PaxDbiQ8WV16.
PeptideAtlasiQ8WV16.
PRIDEiQ8WV16.

Protocols and materials databases

DNASUi26094.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358377; ENSP00000351147; ENSG00000119599. [Q8WV16-1]
ENST00000394234; ENSP00000377781; ENSG00000119599. [Q8WV16-4]
ENST00000509153; ENSP00000426178; ENSG00000119599. [Q8WV16-3]
ENST00000555042; ENSP00000452131; ENSG00000119599. [Q8WV16-5]
GeneIDi26094.
KEGGihsa:26094.
UCSCiuc001xng.4. human. [Q8WV16-1]

Organism-specific databases

CTDi26094.
DisGeNETi26094.
GeneCardsiDCAF4.
HGNCiHGNC:20229. DCAF4.
HPAiHPA047276.
MIMi616372. gene.
neXtProtiNX_Q8WV16.
OpenTargetsiENSG00000119599.
PharmGKBiPA165478762.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2695. Eukaryota.
ENOG410XQIU. LUCA.
GeneTreeiENSGT00390000009546.
HOGENOMiHOG000065756.
HOVERGENiHBG054724.
InParanoidiQ8WV16.
KOiK11799.
OMAiYLMASDM.
OrthoDBiEOG091G06QG.
PhylomeDBiQ8WV16.
TreeFamiTF332050.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:ENSG00000119599-MONOMER.
SignaLinkiQ8WV16.

Miscellaneous databases

ChiTaRSiDCAF4. human.
EvolutionaryTraceiQ8WV16.
GenomeRNAii26094.
PROiQ8WV16.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000119599.
CleanExiHS_WDR21A.
ExpressionAtlasiQ8WV16. baseline and differential.
GenevisibleiQ8WV16. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDCAF4_HUMAN
AccessioniPrimary (citable) accession number: Q8WV16
Secondary accession number(s): B4DUT6
, G3V522, Q86U31, Q8IV10, Q96K22, Q9Y4P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: October 17, 2006
Last modified: November 2, 2016
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.