Q8WV16 (DCAF4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DDB1- and CUL4-associated factor 4

Alternative name(s):
WD repeat-containing protein 21A

Gene names

Name:	DCAF4
Synonyms:	WDR21, WDR21A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. Ref.5
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with DDB1 and CUL4A. Ref.5
Sequence similarities	Contains 2 WD repeats.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat WD repeat
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein ubiquitination Inferred by curator Ref.5. Source: UniProtKB
Cellular component	CUL4 RING ubiquitin ligase complex Inferred from direct assay Ref.5. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WV16-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WV16-2)

The sequence of this isoform differs from the canonical sequence as follows:
     432-433: VG → GT
     434-495: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 495

495

DDB1- and CUL4-associated factor 4

PRO_0000051368

Regions

Repeat

368 – 407

40

WD 1

Repeat

410 – 451

42

WD 2

Natural variations

Alternative sequence

432 – 433

2

VG → GT in isoform 2.

VSP_020986

Alternative sequence

434 – 495

62

Missing in isoform 2.

VSP_020987

Natural variant

22

1

W → C.
Corresponds to variant rs2302588 [ dbSNP | Ensembl ].

VAR_027908

Natural variant

193

1

K → T. Ref.3 Ref.4
Corresponds to variant rs17856582 [ dbSNP | Ensembl ].

VAR_027909

Natural variant

266

1

N → S.
Corresponds to variant rs7155812 [ dbSNP | Ensembl ].

VAR_027910

Natural variant

334

1

L → F. Ref.3 Ref.4
Corresponds to variant rs17856583 [ dbSNP | Ensembl ].

VAR_027911

Natural variant

345

1

S → C.
Corresponds to variant rs3815460 [ dbSNP | Ensembl ].

VAR_027912

Natural variant

439

1

R → I. Ref.3
Corresponds to variant rs17856584 [ dbSNP | Ensembl ].

VAR_027913

Experimental info

Sequence conflict

4

1

S → G in CAB45748. Ref.4

Sequence conflict

35

1

R → Q in BAB55337. Ref.1

Sequence conflict

45

1

H → Y in BAB55337. Ref.1

Sequence conflict

65

1

E → G in BAB55337. Ref.1

Sequence conflict

269

1

P → PA in BAB55337. Ref.1

Sequence conflict

328 – 334

7

Missing in BAB55337. Ref.1

Secondary structure

1

.

.

.

495

Helix Strand Turn

Details...

Helix

126 – 133

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: F104B7F1FC6B5C33	FASTA	495	55,694
	10 20 30 40 50 60 MNKSRWQSRR RHGRRSHQQN PWFRLRDSED RSDSRAAQPA HDSGHGDDES PSTSSGTAGT 70 80 90 100 110 120 SSVPELPGFY FDPEKKRYFR LLPGHNNCNP LTKESIRQKE MESKRLRLLQ EEDRRKKIAR 130 140 150 160 170 180 MGFNASSMLR KSQLGFLNVT NYCHLAHELR LSCMERKKVQ IRSMDPSALA SDRFNLILAD 190 200 210 220 230 240 TNSDRLFTVN DVKVGGSKYG IINLQSLKTP TLKVFMHENL YFTNRKVNSV CWASLNHLDS 250 260 270 280 290 300 HILLCLMGLA ETPGCATLLP ASLFVNSHPG IDRPGMLCSF RIPGAWSCAW SLNIQANNCF 310 320 330 340 350 360 STGLSRRVLL TNVVTGHRQS FGTNSDVLAQ QFALMAPLLF NGCRSGEIFA IDLRCGNQGK 370 380 390 400 410 420 GWKATRLFHD SAVTSVRILQ DEQYLMASDM AGKIKLWDLR TTKCVRQYEG HVNEYAYLPL 430 440 450 460 470 480 HVHEEEGILV AVGQDCYTRI WSLHDARLLR TIPSPYPASK ADIPSVAFSS RLGGSRGAPG 490 LLMAVGQDLY CYSYS
	Isoform 2 [UniParc]. Checksum: 109E83E34CC003B2	FASTA	433	48,933
	10 20 30 40 50 60 MNKSRWQSRR RHGRRSHQQN PWFRLRDSED RSDSRAAQPA HDSGHGDDES PSTSSGTAGT 70 80 90 100 110 120 SSVPELPGFY FDPEKKRYFR LLPGHNNCNP LTKESIRQKE MESKRLRLLQ EEDRRKKIAR 130 140 150 160 170 180 MGFNASSMLR KSQLGFLNVT NYCHLAHELR LSCMERKKVQ IRSMDPSALA SDRFNLILAD 190 200 210 220 230 240 TNSDRLFTVN DVKVGGSKYG IINLQSLKTP TLKVFMHENL YFTNRKVNSV CWASLNHLDS 250 260 270 280 290 300 HILLCLMGLA ETPGCATLLP ASLFVNSHPG IDRPGMLCSF RIPGAWSCAW SLNIQANNCF 310 320 330 340 350 360 STGLSRRVLL TNVVTGHRQS FGTNSDVLAQ QFALMAPLLF NGCRSGEIFA IDLRCGNQGK 370 380 390 400 410 420 GWKATRLFHD SAVTSVRILQ DEQYLMASDM AGKIKLWDLR TTKCVRQYEG HVNEYAYLPL 430 HVHEEEGILV AGT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovarian carcinoma.
[2]	"Full-length cDNA libraries and normalization." Li W.B., Gruber C., Jessee J., Polayes D. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Neuroblastoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS THR-193; PHE-334 AND ILE-439. Tissue: Uterus.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-495 (ISOFORM 2), VARIANTS THR-193 AND PHE-334. Tissue: Testis.
[5]	"A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1." Jin J., Arias E.E., Chen J., Harper J.W., Walter J.C. Mol. Cell 23:709-721(2006) [PubMed: 16949367] [Abstract] Cited for: FUNCTION, INTERACTION WITH DDB1 AND CUL4A, IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AK027745 mRNA. Translation: BAB55337.1.
BX247962 mRNA. Translation: CAD62301.1.
BC018979 mRNA. Translation: AAH18979.1.
AL080157 mRNA. Translation: CAB45748.1.

IPI

IPI00385324.
IPI00790322.

PIR

T12541.

RefSeq

NP_001156980.1. NM_001163508.1.
NP_001156981.1. NM_001163509.1.
NP_056419.2. NM_015604.3.
NP_851937.1. NM_181340.2.
NP_851938.2. NM_181341.2.

UniGene

Hs.331491.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3I8C	X-ray	2.80	B	124-136	[»]

ProteinModelPortal

Q8WV16.

SMR

Q8WV16. Positions 282-470.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48761N.

IntAct

Q8WV16. 2 interactions.

STRING

Q8WV16.

PTM databases

PhosphoSite

Q8WV16.

Polymorphism databases

DMDM

116242844.

Proteomic databases

PRIDE

Q8WV16.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000358377; ENSP00000351147; ENSG00000119599.

GeneID

26094.

KEGG

hsa:26094.

UCSC

uc001xng.1. human.
uc001xnk.2. human.

Organism-specific databases

CTD

26094.

GeneCards

GC14P073393.

H-InvDB

HIX0011786.

HGNC

HGNC:20229. DCAF4.

HPA

HPA047276.

neXtProt

NX_Q8WV16.

PharmGKB

PA134944342.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00390000009546.

HOGENOM

HBG445161.

HOVERGEN

HBG054724.

InParanoid

Q8WV16.

OMA

NIQANNC.

OrthoDB

EOG4N8R4Q.

PhylomeDB

Q8WV16.

Gene expression databases

ArrayExpress

Q8WV16.

Bgee

Q8WV16.

CleanEx

HS_WDR21A.

Genevestigator

Q8WV16.

GermOnline

ENSG00000119599. Homo sapiens.

Family and domain databases

InterPro

IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR011046. WD40_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.

KO

K11799.

Pfam

PF00400. WD40. 1 hit.
[Graphical view]

SMART

SM00320. WD40. 2 hits.
[Graphical view]

SUPFAM

SSF50978. WD40_like. 1 hit.

PROSITE

PS00678. WD_REPEATS_1. False negative.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

48041.

Entry information

Entry name

DCAF4_HUMAN

Accession

Primary (citable) accession number: Q8WV16
Secondary accession number(s): Q86U31, Q96K22, Q9Y4P5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.