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Protein

DDB1- and CUL4-associated factor 4

Gene

DCAF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-8951664 Neddylation
SignaLinkiQ8WV16
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
DDB1- and CUL4-associated factor 4
Alternative name(s):
WD repeat-containing protein 21A
Gene namesi
Name:DCAF4
Synonyms:WDR21, WDR21A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000119599.16
HGNCiHGNC:20229 DCAF4
MIMi616372 gene
neXtProtiNX_Q8WV16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi26094
OpenTargetsiENSG00000119599
PharmGKBiPA165478762

Polymorphism and mutation databases

BioMutaiDCAF4
DMDMi116242844

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000513681 – 495DDB1- and CUL4-associated factor 4Add BLAST495

Proteomic databases

MaxQBiQ8WV16
PaxDbiQ8WV16
PeptideAtlasiQ8WV16
PRIDEiQ8WV16
ProteomicsDBi74734
74735 [Q8WV16-2]
74736 [Q8WV16-3]

PTM databases

iPTMnetiQ8WV16
PhosphoSitePlusiQ8WV16

Expressioni

Gene expression databases

BgeeiENSG00000119599
CleanExiHS_WDR21A
ExpressionAtlasiQ8WV16 baseline and differential
GenevisibleiQ8WV16 HS

Organism-specific databases

HPAiHPA047276

Interactioni

Subunit structurei

Interacts with DDB1 and CUL4A.1 Publication

Protein-protein interaction databases

BioGridi117545, 30 interactors
DIPiDIP-48761N
IntActiQ8WV16, 7 interactors
STRINGi9606.ENSP00000351147

Structurei

Secondary structure

1495
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi126 – 134Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3I8CX-ray2.80B124-136[»]
ProteinModelPortaliQ8WV16
SMRiQ8WV16
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WV16

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati368 – 407WD 1Add BLAST40
Repeati410 – 451WD 2Add BLAST42

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2695 Eukaryota
ENOG410XQIU LUCA
GeneTreeiENSGT00390000009546
HOGENOMiHOG000065756
HOVERGENiHBG054724
InParanoidiQ8WV16
KOiK11799
OMAiYLMASDM
OrthoDBiEOG091G06QG
PhylomeDBiQ8WV16
TreeFamiTF332050

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
SMARTiView protein in SMART
SM00320 WD40, 2 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 1 hit
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WV16-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNKSRWQSRR RHGRRSHQQN PWFRLRDSED RSDSRAAQPA HDSGHGDDES
60 70 80 90 100
PSTSSGTAGT SSVPELPGFY FDPEKKRYFR LLPGHNNCNP LTKESIRQKE
110 120 130 140 150
MESKRLRLLQ EEDRRKKIAR MGFNASSMLR KSQLGFLNVT NYCHLAHELR
160 170 180 190 200
LSCMERKKVQ IRSMDPSALA SDRFNLILAD TNSDRLFTVN DVKVGGSKYG
210 220 230 240 250
IINLQSLKTP TLKVFMHENL YFTNRKVNSV CWASLNHLDS HILLCLMGLA
260 270 280 290 300
ETPGCATLLP ASLFVNSHPG IDRPGMLCSF RIPGAWSCAW SLNIQANNCF
310 320 330 340 350
STGLSRRVLL TNVVTGHRQS FGTNSDVLAQ QFALMAPLLF NGCRSGEIFA
360 370 380 390 400
IDLRCGNQGK GWKATRLFHD SAVTSVRILQ DEQYLMASDM AGKIKLWDLR
410 420 430 440 450
TTKCVRQYEG HVNEYAYLPL HVHEEEGILV AVGQDCYTRI WSLHDARLLR
460 470 480 490
TIPSPYPASK ADIPSVAFSS RLGGSRGAPG LLMAVGQDLY CYSYS
Length:495
Mass (Da):55,694
Last modified:October 17, 2006 - v3
Checksum:iF104B7F1FC6B5C33
GO
Isoform 2 (identifier: Q8WV16-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     432-433: VG → GT
     434-495: Missing.

Note: No experimental confirmation available.
Show »
Length:433
Mass (Da):48,933
Checksum:i109E83E34CC003B2
GO
Isoform 3 (identifier: Q8WV16-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     118-178: Missing.
     269-269: P → PA

Note: No experimental confirmation available.
Show »
Length:435
Mass (Da):48,798
Checksum:iB1C5BAC49BDD9B60
GO
Isoform 4 (identifier: Q8WV16-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Show »
Length:395
Mass (Da):44,161
Checksum:i6BC9F63E4A6D85D3
GO
Isoform 5 (identifier: Q8WV16-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     269-269: P → PA
     329-335: Missing.

Note: No experimental confirmation available.
Show »
Length:489
Mass (Da):54,975
Checksum:i362DF3B15C3D7656
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4S → G in CAB45748 (PubMed:17974005).Curated1
Sequence conflicti35R → Q in BAB55337 (PubMed:14702039).Curated1
Sequence conflicti45H → Y in BAB55337 (PubMed:14702039).Curated1
Sequence conflicti65E → G in BAB55337 (PubMed:14702039).Curated1

Polymorphismi

An intronic G-to-A transition (rs2535913) has been associated with leukocyte telomere length. The minor A allele is associated with shorter telomeres and lower expression in lymphoblastoid cells and in sun-exposed skin (PubMed:25624462).1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02790822W → C. Corresponds to variant dbSNP:rs2302588Ensembl.1
Natural variantiVAR_027909193K → T2 PublicationsCorresponds to variant dbSNP:rs17856582Ensembl.1
Natural variantiVAR_027910266N → S. Corresponds to variant dbSNP:rs7155812Ensembl.1
Natural variantiVAR_027911334L → F2 PublicationsCorresponds to variant dbSNP:rs17856583Ensembl.1
Natural variantiVAR_027912345S → C. Corresponds to variant dbSNP:rs3815460Ensembl.1
Natural variantiVAR_027913439R → I1 PublicationCorresponds to variant dbSNP:rs17856584Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0454271 – 100Missing in isoform 4. 1 PublicationAdd BLAST100
Alternative sequenceiVSP_047171118 – 178Missing in isoform 3. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_047172269P → PA in isoform 3 and isoform 5. 1 Publication1
Alternative sequenceiVSP_047173329 – 335Missing in isoform 5. 1 Publication7
Alternative sequenceiVSP_020986432 – 433VG → GT in isoform 2. 1 Publication2
Alternative sequenceiVSP_020987434 – 495Missing in isoform 2. 1 PublicationAdd BLAST62

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX247962 mRNA Translation: CAD62301.1
AK027745 mRNA Translation: BAB55337.1
AK300786 mRNA Translation: BAG62448.1
AC007160 Genomic DNA No translation available.
AL442663 Genomic DNA No translation available.
BC018979 mRNA Translation: AAH18979.1
BC035901 mRNA Translation: AAH35901.1
AL080157 mRNA Translation: CAB45748.1
CCDSiCCDS41968.2 [Q8WV16-3]
CCDS55926.1 [Q8WV16-5]
CCDS9809.1 [Q8WV16-1]
CCDS9810.1 [Q8WV16-4]
PIRiT12541
RefSeqiNP_001156980.1, NM_001163508.1 [Q8WV16-5]
NP_001156981.1, NM_001163509.1
NP_056419.2, NM_015604.3 [Q8WV16-1]
NP_851937.1, NM_181340.2 [Q8WV16-4]
NP_851938.2, NM_181341.2 [Q8WV16-3]
XP_016876696.1, XM_017021207.1 [Q8WV16-1]
XP_016876697.1, XM_017021208.1 [Q8WV16-1]
UniGeneiHs.331491

Genome annotation databases

EnsembliENST00000358377; ENSP00000351147; ENSG00000119599 [Q8WV16-1]
ENST00000394234; ENSP00000377781; ENSG00000119599 [Q8WV16-4]
ENST00000509153; ENSP00000426178; ENSG00000119599 [Q8WV16-3]
ENST00000555042; ENSP00000452131; ENSG00000119599 [Q8WV16-5]
GeneIDi26094
KEGGihsa:26094
UCSCiuc001xng.4 human [Q8WV16-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDCAF4_HUMAN
AccessioniPrimary (citable) accession number: Q8WV16
Secondary accession number(s): B4DUT6
, G3V522, Q86U31, Q8IV10, Q96K22, Q9Y4P5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: October 17, 2006
Last modified: June 20, 2018
This is version 142 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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