Q8WUY9 (DEP1B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DEP domain-containing protein 1B

Alternative name(s):
HBV X-transactivated gene 8 protein
HBV XAg-transactivated protein 8

Gene names

Name:	DEPDC1B
Synonyms:	XTP8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities	Contains 1 DEP domain. Contains 1 Rho-GAP domain.
Sequence caution	The sequence AAH10904.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA91529.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC regulation of small GTPase mediated signal transduction Traceable author statement. Source: Reactome small GTPase mediated signal transduction Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WUY9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WUY9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     414-475: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	DEP domain-containing protein 1B		PRO_0000284790
Regions
	Domain	24 – 108	85	DEP
	Domain	201 – 393	193	Rho-GAP
	Compositional bias	468 – 475	8	Poly-Lys
Amino acid modifications
	Modified residue	436	1	Phosphoserine Ref.8
Natural variations
	Alternative sequence	414 – 475	62	Missing in isoform 2.		VSP_042922
	Natural variant	332	1	R → M. Ref.2 Ref.6 Corresponds to variant rs17856590 [ dbSNP | Ensembl ].		VAR_031819
	Natural variant	395	1	V → L. Ref.2 Ref.6 Corresponds to variant rs17851707 [ dbSNP | Ensembl ].		VAR_031820
Experimental info
	Sequence conflict	272	1	F → C in AAN31126. Ref.1
	Sequence conflict	272	1	F → C in BAA92089. Ref.3
	Sequence conflict	380	1	R → K in AAN31126. Ref.1
	Sequence conflict	402	1	S → P in BAA92089. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 17, 2007. Version 2. Checksum: E640F8034BD308C6	FASTA	529	61,771
	10 20 30 40 50 60 MEHRIVGPGP YRATRLWNET VELFRAKMPL RKHRCRFKSY EHCFTAAEAV DWLHELLRCS 70 80 90 100 110 120 QNFGPEVTRK QTVQLLKKFL KNHVIEDIKG KWGEEDFEDN RHLYRFPPSS PLKPYPKKPP 130 140 150 160 170 180 NQKDVIKFPE WNDLPPGTSQ ENIPVRPVVM NSEMWYKRHS IAIGEVPACR LVHRRQLTEA 190 200 210 220 230 240 NVEEIWKSMT LSYLQKILGL DSLEEVLDVK LVNSKFIIHN VYSVSKQGVV ILDDKSKELP 250 260 270 280 290 300 HWVLSAMKCL ANWPNCSDLK QPMYLGFEKD VFKTIADYYG HLKEPLLTFH LFDAFVSVLG 310 320 330 340 350 360 LLQKEKVAVE AFQICCLLLP PENRRKLQLL MRMMARICLN KEMPPLCDGF GTRTLMVQTF 370 380 390 400 410 420 SRCILCSKDE VDLDELLAAR LVTFLMDNYQ EILKVPLALQ TSIEERVAHL RRVQIKYPGA 430 440 450 460 470 480 DMDITLSAPS FCRQISPEEF EYQRSYGSQE PLAALLEEVI TDAKLSNKEK KKKLKQFQKS 490 500 510 520 YPEVYQERFP TPESAALLFP EKPKPKPQLL MWALKKPFQP FQRTRSFRM
	Isoform 2 [UniParc]. Checksum: C549F29AF83121F8	FASTA	467	54,726
	10 20 30 40 50 60 MEHRIVGPGP YRATRLWNET VELFRAKMPL RKHRCRFKSY EHCFTAAEAV DWLHELLRCS 70 80 90 100 110 120 QNFGPEVTRK QTVQLLKKFL KNHVIEDIKG KWGEEDFEDN RHLYRFPPSS PLKPYPKKPP 130 140 150 160 170 180 NQKDVIKFPE WNDLPPGTSQ ENIPVRPVVM NSEMWYKRHS IAIGEVPACR LVHRRQLTEA 190 200 210 220 230 240 NVEEIWKSMT LSYLQKILGL DSLEEVLDVK LVNSKFIIHN VYSVSKQGVV ILDDKSKELP 250 260 270 280 290 300 HWVLSAMKCL ANWPNCSDLK QPMYLGFEKD VFKTIADYYG HLKEPLLTFH LFDAFVSVLG 310 320 330 340 350 360 LLQKEKVAVE AFQICCLLLP PENRRKLQLL MRMMARICLN KEMPPLCDGF GTRTLMVQTF 370 380 390 400 410 420 SRCILCSKDE VDLDELLAAR LVTFLMDNYQ EILKVPLALQ TSIEERVAHL RRVQFQKSYP 430 440 450 460 EVYQERFPTP ESAALLFPEK PKPKPQLLMW ALKKPFQPFQ RTRSFRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel transcript in human breast cancer cells." Gokhale P.C., Kumar D., Monia B.P., Rahman A., Dritschilo A., Kasid U. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Mammary cancer.
[2]	"Cloning and identification of human gene 8 transactivated by hepatitis B virus X antigen." Liu Y., Cheng J., Lu Y., Wang G., Zhang L., Chen J., Li L. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS MET-332 AND LEU-395.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Placenta.
[4]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS MET-332 AND LEU-395. Tissue: Pancreas.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[8]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-436, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF303178 mRNA. Translation: AAN31126.1. AF488828 mRNA. Translation: AAO49477.1. AF490257 mRNA. Translation: AAO85465.1. AK001166 mRNA. Translation: BAA91529.1. Different initiation. AK002114 mRNA. Translation: BAA92089.1. AK290684 mRNA. Translation: BAF83373.1. AK300782 mRNA. Translation: BAG62446.1. AC016591 Genomic DNA. No translation available. AC109133 Genomic DNA. No translation available. CH471123 Genomic DNA. Translation: EAW54998.1. BC010904 mRNA. Translation: AAH10904.1. Different initiation. BC019075 mRNA. Translation: AAH19075.1.
IPI	IPI00293897. IPI00909567.
RefSeq	NP_001138680.1. NM_001145208.1. NP_060839.2. NM_018369.2.
UniGene	Hs.741331.
3D structure databases
ProteinModelPortal	Q8WUY9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8WUY9. 1 interaction.
STRING	9606.ENSP00000265036.
PTM databases
PhosphoSite	Q8WUY9.
Polymorphism databases
DMDM	145558895.
Proteomic databases
PaxDb	Q8WUY9.
PRIDE	Q8WUY9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265036; ENSP00000265036; ENSG00000035499. ENST00000453022; ENSP00000389101; ENSG00000035499.
GeneID	55789.
KEGG	hsa:55789.
UCSC	uc003jsh.3. human.
Organism-specific databases
CTD	55789.
GeneCards	GC05M059928.
HGNC	HGNC:24902. DEPDC1B.
HPA	HPA038255.
neXtProt	NX_Q8WUY9.
PharmGKB	PA134941087.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG148822.
HOGENOM	HOG000013064.
HOVERGEN	HBG059792.
InParanoid	Q8WUY9.
OMA	FSHCILC.
OrthoDB	EOG4DV5M2.
PhylomeDB	Q8WUY9.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	Q8WUY9.
Bgee	Q8WUY9.
CleanEx	HS_DEPDC1B.
Genevestigator	Q8WUY9.
Family and domain databases
Gene3D	1.10.10.10. 1 hit. 1.10.555.10. 1 hit.
InterPro	IPR000591. DEP_dom. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00610. DEP. 1 hit. PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00049. DEP. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50186. DEP. 1 hit. PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	55789.
NextBio	60896.

Entry information

Entry name

DEP1B_HUMAN

Accession

Primary (citable) accession number: Q8WUY9
Secondary accession number(s): A8K3R9 , B4DUT4, Q86WJ3, Q8IZY6, Q9NUN3, Q9NW57

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
Last sequence update:	April 17, 2007
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.