Q8WUW1 (BRK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein BRICK1
Short name=BRK1

Gene names

Name:	BRK1
Synonyms:	C3orf10
ORF Names:	HSPC300, MDS027

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	75 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in regulation of actin and microtubule organization. Part of a WAVE complex that activates the Arp2/3 complex. Ref.7
Subunit structure	Homotrimer when in free form. Directly interacts with WASF2. Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. Ref.6 Ref.7 Ref.11
Subcellular location	Cytoplasm › cytoskeleton By similarity.
Sequence similarities	Belongs to the BRK1 family.
Sequence caution	The sequence AAF28978.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WUW1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WUW1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     75-75: T → TRTVPCCCWEVALHNTGHMGKAPAAFSSFLSP

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 75

74

Protein BRICK1

PRO_0000283646

Regions

Coiled coil

41 – 72

32

Potential

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.8

Modified residue

63

1

Phosphotyrosine Ref.9

Natural variations

Alternative sequence

75

1

T → TRTVPCCCWEVALHNTGHMG KAPAAFSSFLSP in isoform 2.

VSP_024350

Secondary structure

1

.

.

.

75

Helix Strand Turn

Details...

Helix

11 – 68

58

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 637B5461A2D96587	FASTA	75	8,745
	10 20 30 40 50 60 MAGQEDPVQR EIHQDWANRE YIEIITSSIK KIADFLNSFD MSCRSRLATL NEKLTALERR 70 IEYIEARVTK GETLT
	Isoform 2 [UniParc]. Checksum: 908E7A141251DFE5	FASTA	106	12,046
	10 20 30 40 50 60 MAGQEDPVQR EIHQDWANRE YIEIITSSIK KIADFLNSFD MSCRSRLATL NEKLTALERR 70 80 90 100 IEYIEARVTK GETLTRTVPC CCWEVALHNT GHMGKAPAAF SSFLSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Xu Q., Duan R., Huo Y., Fan B., Zhang K., Wu D. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Fetal brain and Lung.
[2]	"Human partial CDS from CD34+ stem cells." Ye M., Zhang Q.-H., Zhou J., Shen Y., Wu X.-Y., Guan Z.Q., Wang L., Fan H.-Y., Mao Y.-F., Dai M., Huang Q.-H., Chen S.-J., Chen Z. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Umbilical cord blood.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Hippocampus.
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung and Lymph.
[6]	"Purification and architecture of the ubiquitous Wave complex." Gautreau A., Ho H.-Y., Li J., Steen H., Gygi S.P., Kirschner M.W. Proc. Natl. Acad. Sci. U.S.A. 101:4379-4383(2004) [PubMed: 15070726] [Abstract] Cited for: INTERACTION WITH WASF2.
[7]	"Free Brick1 is a trimeric precursor in the assembly of a functional wave complex." Derivery E., Fink J., Martin D., Houdusse A., Piel M., Stradal T.E., Louvard D., Gautreau A. PLoS ONE 3:E2462-E2462(2008) [PubMed: 18560548] [Abstract] Cited for: FUNCTION, SUBUNIT.
[8]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A., Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D., Wiley H.S., Qian W.-J. J. Proteome Res. 8:3852-3861(2009) [PubMed: 19534553] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-63, MASS SPECTROMETRY. Tissue: Mammary epithelium.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Structure and control of the actin regulatory WAVE complex." Chen Z., Borek D., Padrick S.B., Gomez T.S., Metlagel Z., Ismail A.M., Umetani J., Billadeau D.D., Otwinowski Z., Rosen M.K. Nature 468:533-538(2010) [PubMed: 21107423] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.29 ANGSTROMS) OF WAVE1 COMPLEX, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY148219 mRNA. Translation: AAN60161.1.
AY148220 mRNA. Translation: AAN60162.1.
AF161418 mRNA. Translation: AAF28978.1. Different initiation.
AK312155 mRNA. Translation: BAG35089.1.
CH471055 Genomic DNA. Translation: EAW64061.1.
BC001067 mRNA. Translation: AAH01067.1.
BC007929 mRNA. Translation: AAH07929.1.
BC019303 mRNA. Translation: AAH19303.1.

IPI

IPI00844425.
IPI01010967.

RefSeq

NP_060932.2. NM_018462.4.

UniGene

Hs.649307.
Hs.707283.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3P8C	X-ray	2.29	E	1-75	[»]

ProteinModelPortal

Q8WUW1.

SMR

Q8WUW1. Positions 10-73.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-41554N.

IntAct

Q8WUW1. 3 interactions.

MINT

MINT-244852.

PTM databases

PhosphoSite

Q8WUW1.

Polymorphism databases

DMDM

74730773.

Proteomic databases

PRIDE

Q8WUW1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

55845.

KEGG

hsa:55845.

UCSC

uc003bvb.1. human.

Organism-specific databases

CTD

55845.

GeneCards

GC03P010162.

HGNC

HGNC:23057. BRK1.

MIM

611183. gene.

neXtProt

NX_Q8WUW1.

PharmGKB

PA134866423.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG21709.

HOVERGEN

HBG107432.

InParanoid

Q8WUW1.

OrthoDB

EOG4CG09X.

PhylomeDB

Q8WUW1.

Gene expression databases

Bgee

Q8WUW1.

CleanEx

HS_C3orf10.

Genevestigator

Q8WUW1.

Family and domain databases

KO

K05752.

ProtoNet

Search...

Other

NextBio

61091.

SOURCE

Search...

Entry information

Entry name

BRK1_HUMAN

Accession

Primary (citable) accession number: Q8WUW1
Secondary accession number(s): B2R5E2, Q9P082

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.