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Protein

Cactin

Gene

CACTIN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of innate immune response. Acts as negative regulator of Toll-like receptor and interferon-regulatory factor (IRF) signaling pathways. Contributes to the regulation of transcriptional activation of NF-kappa-B target genes in response to endogenous proinflammatory stimuli. May play a role during early embryonic development. Probably involved in pre-mRNA splicing.1 Publication

GO - Molecular functioni

  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

  • cellular response to interleukin-1 Source: UniProtKB
  • cellular response to lipopolysaccharide Source: UniProtKB
  • cellular response to tumor necrosis factor Source: UniProtKB
  • innate immune response Source: UniProtKB-KW
  • mRNA splicing, via spliceosome Source: UniProtKB
  • multicellular organismal development Source: UniProtKB-KW
  • negative regulation of interferon-beta production Source: UniProtKB
  • negative regulation of interleukin-8 production Source: UniProtKB
  • negative regulation of lipopolysaccharide-mediated signaling pathway Source: UniProtKB
  • negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • negative regulation of protein phosphorylation Source: UniProtKB
  • negative regulation of toll-like receptor signaling pathway Source: UniProtKB
  • negative regulation of tumor necrosis factor production Source: UniProtKB
  • negative regulation of type I interferon-mediated signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Immunity, Innate immunity, mRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Cactin
Alternative name(s):
Renal carcinoma antigen NY-REN-24
Gene namesi
Name:CACTIN
Synonyms:C19orf29
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:29938. CACTIN.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Nuclear localization with a speckled expression pattern in some cells. Colocalizes with NFKBIL1 in the nucleus.

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • cytoplasm Source: HPA
  • extracellular exosome Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134932683.

Polymorphism and mutation databases

BioMutaiCACTIN.
DMDMi322510133.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 758758CactinPRO_0000231617Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei559 – 5591Phosphotyrosine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8WUQ7.
PaxDbiQ8WUQ7.
PRIDEiQ8WUQ7.

PTM databases

PhosphoSiteiQ8WUQ7.

Expressioni

Gene expression databases

BgeeiQ8WUQ7.
CleanExiHS_C19orf29.
ExpressionAtlasiQ8WUQ7. baseline and differential.
GenevisibleiQ8WUQ7. HS.

Organism-specific databases

HPAiHPA042504.
HPA042548.

Interactioni

Subunit structurei

Interacts (via N-terminal domain) with NFKBIL1; the interaction occurs in a proinflammatory-independent manner. Does not interact with RELA NF-kappa-B subunit. Identified in the spliceosome C complex.2 Publications

Protein-protein interaction databases

BioGridi121836. 7 interactions.
IntActiQ8WUQ7. 2 interactions.
MINTiMINT-4654969.
STRINGi9606.ENSP00000248420.

Structurei

3D structure databases

ProteinModelPortaliQ8WUQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili135 – 18551Sequence AnalysisAdd
BLAST
Coiled coili225 – 27753Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi101 – 1077Poly-Arg
Compositional biasi129 – 1335Poly-Ala

Sequence similaritiesi

Belongs to the CACTIN family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG290728.
GeneTreeiENSGT00390000000097.
HOVERGENiHBG079755.
InParanoidiQ8WUQ7.
OrthoDBiEOG7XM2Z3.
PhylomeDBiQ8WUQ7.
TreeFamiTF300906.

Family and domain databases

InterProiIPR019134. Cactin_C.
IPR018816. Cactin_central.
[Graphical view]
PfamiPF10312. Cactin_mid. 1 hit.
PF09732. CactinC_cactus. 1 hit.
[Graphical view]
SMARTiSM01050. CactinC_cactus. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WUQ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRDTRSRSR SAGRRGRRRQ SQSGSRSRSR SHGRRNRRRR EDEGRRRRRR
60 70 80 90 100
RSRERRSDSE EERWQRSGMR SRSPPRPKWH SRDGSSQSDS GEEQSRGQWA
110 120 130 140 150
RRRRRARSWS PSSSASSSAS PGRSQSPRAA AAALSQQQSL QERLRLREER
160 170 180 190 200
KQQEELMKAF ETPEEKRARR LAKKEAKERK KREKMGWGEE YMGYTNTDNP
210 220 230 240 250
FGDNNLLGTF IWNKALEKKG ISHLEEKELK ERNKRIQEDN RLELQKVKQL
260 270 280 290 300
RLEREREKAM REQELEMLQR EKEAEHFKTW EEQEDNFHLQ QAKLRSKIRI
310 320 330 340 350
RDGRAKPIDL LAKYISAEDD DLAVEMHEPY TFLNGLTVAD MEDLLEDIQV
360 370 380 390 400
YMELEQGKNA DFWRDMTTIT EDEISKLRKL EASGKGPGER REGVNASVSS
410 420 430 440 450
DVQSVFKGKT YNQLQVIFQG IEGKIRAGGP NLDMGYWESL LQQLRAHMAR
460 470 480 490 500
ARLRERHQDV LRQKLYKLKQ EQGVESEPLF PILKQEPQSP SRSLEPEDAA
510 520 530 540 550
PTPPGPSSEG GPAEAEVDGA TPTEGDGDGD GEGEGEGEAV LMEEDLIQQS
560 570 580 590 600
LDDYDAGRYS PRLLTAHELP LDAHVLEPDE DLQRLQLSRQ QLQVTGDASE
610 620 630 640 650
SAEDIFFRRA KEGMGQDEAQ FSVEMPLTGK AYLWADKYRP RKPRFFNRVH
660 670 680 690 700
TGFEWNKYNQ THYDFDNPPP KIVQGYKFNI FYPDLIDKRS TPEYFLEACA
710 720 730 740 750
DNKDFAILRF HAGPPYEDIA FKIVNREWEY SHRHGFRCQF ANGIFQLWFH

FKRYRYRR
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Length:758
Mass (Da):88,702
Last modified:February 8, 2011 - v3
Checksum:iF540F151EB96BEFB
GO
Isoform 2 (identifier: Q8WUQ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     758-758: R → RPGPCWRPIR...LAYWQDRMFF

Note: No experimental confirmation available.
Show »
Length:935
Mass (Da):107,938
Checksum:iE7238F52D6384E50
GO

Sequence cautioni

The sequence AAC24305.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAC32903.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAD42868.1 differs from that shown. Reason: Frameshift at position 711. Curated
The sequence AAH04262.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence EAW69298.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti499 – 4991A → V in AAH19848 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei758 – 7581R → RPGPCWRPIRHCRRDPLWTP TLCRDWPPTHPVLAGGVHFP AAGIPPPGLLTGPWSMRPVT PSFAHIRTVAPSHSPFSGQE GRGPHGCHSPGRSGPAGRLV LQHPTGTSPTEAKRKVPPGP PEGHPTSPVTSPRPPTAPPR HPASSGNSSVCFSKKTCRWE KKSFVLMELAYWQDRMFF in isoform 2. CuratedVSP_017856

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY917150 mRNA. Translation: AAX84551.1.
AC005175 Genomic DNA. Translation: AAC24305.1. Sequence problems.
AC005542 Genomic DNA. Translation: AAC32903.1. Sequence problems.
AF155102 mRNA. Translation: AAD42868.1. Frameshift.
CH471139 Genomic DNA. Translation: EAW69298.1. Frameshift.
BC004262 mRNA. Translation: AAH04262.1. Different initiation.
BC019848 mRNA. Translation: AAH19848.1.
CCDSiCCDS45920.1. [Q8WUQ7-1]
PIRiT02672.
RefSeqiNP_001074012.1. NM_001080543.1. [Q8WUQ7-1]
NP_067054.1. NM_021231.1. [Q8WUQ7-1]
UniGeneiHs.128425.

Genome annotation databases

EnsembliENST00000221899; ENSP00000221899; ENSG00000105298. [Q8WUQ7-1]
ENST00000248420; ENSP00000248420; ENSG00000105298. [Q8WUQ7-1]
ENST00000429344; ENSP00000415078; ENSG00000105298. [Q8WUQ7-1]
ENST00000585942; ENSP00000465751; ENSG00000105298. [Q8WUQ7-1]
GeneIDi58509.
KEGGihsa:58509.
UCSCiuc002lyh.3. human. [Q8WUQ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY917150 mRNA. Translation: AAX84551.1.
AC005175 Genomic DNA. Translation: AAC24305.1. Sequence problems.
AC005542 Genomic DNA. Translation: AAC32903.1. Sequence problems.
AF155102 mRNA. Translation: AAD42868.1. Frameshift.
CH471139 Genomic DNA. Translation: EAW69298.1. Frameshift.
BC004262 mRNA. Translation: AAH04262.1. Different initiation.
BC019848 mRNA. Translation: AAH19848.1.
CCDSiCCDS45920.1. [Q8WUQ7-1]
PIRiT02672.
RefSeqiNP_001074012.1. NM_001080543.1. [Q8WUQ7-1]
NP_067054.1. NM_021231.1. [Q8WUQ7-1]
UniGeneiHs.128425.

3D structure databases

ProteinModelPortaliQ8WUQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121836. 7 interactions.
IntActiQ8WUQ7. 2 interactions.
MINTiMINT-4654969.
STRINGi9606.ENSP00000248420.

PTM databases

PhosphoSiteiQ8WUQ7.

Polymorphism and mutation databases

BioMutaiCACTIN.
DMDMi322510133.

Proteomic databases

MaxQBiQ8WUQ7.
PaxDbiQ8WUQ7.
PRIDEiQ8WUQ7.

Protocols and materials databases

DNASUi58509.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000221899; ENSP00000221899; ENSG00000105298. [Q8WUQ7-1]
ENST00000248420; ENSP00000248420; ENSG00000105298. [Q8WUQ7-1]
ENST00000429344; ENSP00000415078; ENSG00000105298. [Q8WUQ7-1]
ENST00000585942; ENSP00000465751; ENSG00000105298. [Q8WUQ7-1]
GeneIDi58509.
KEGGihsa:58509.
UCSCiuc002lyh.3. human. [Q8WUQ7-1]

Organism-specific databases

CTDi58509.
GeneCardsiGC19M003610.
H-InvDBHIX0014643.
HIX0027556.
HGNCiHGNC:29938. CACTIN.
HPAiHPA042504.
HPA042548.
neXtProtiNX_Q8WUQ7.
PharmGKBiPA134932683.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG290728.
GeneTreeiENSGT00390000000097.
HOVERGENiHBG079755.
InParanoidiQ8WUQ7.
OrthoDBiEOG7XM2Z3.
PhylomeDBiQ8WUQ7.
TreeFamiTF300906.

Miscellaneous databases

GeneWikiiC19orf29.
GenomeRNAii58509.
NextBioi65027.
PROiQ8WUQ7.

Gene expression databases

BgeeiQ8WUQ7.
CleanExiHS_C19orf29.
ExpressionAtlasiQ8WUQ7. baseline and differential.
GenevisibleiQ8WUQ7. HS.

Family and domain databases

InterProiIPR019134. Cactin_C.
IPR018816. Cactin_central.
[Graphical view]
PfamiPF10312. Cactin_mid. 1 hit.
PF09732. CactinC_cactus. 1 hit.
[Graphical view]
SMARTiSM01050. CactinC_cactus. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cai Y., Yan Y.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 123-758.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 69-758.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 469-758 (ISOFORM 1).
    Tissue: Cervix and Uterus.
  5. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 534-758 (ISOFORM 1), IDENTIFICATION AS A RENAL CANCER ANTIGEN.
    Tissue: Renal cell carcinoma.
  6. "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
    Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
    RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
  7. "Cactin targets the MHC class III protein IkappaB-like (IkappaBL) and inhibits NF-kappaB and interferon-regulatory factor signaling pathways."
    Atzei P., Gargan S., Curran N., Moynagh P.N.
    J. Biol. Chem. 285:36804-36817(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NFKBIL1, SUBCELLULAR LOCATION.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-559, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiCATIN_HUMAN
AccessioniPrimary (citable) accession number: Q8WUQ7
Secondary accession number(s): A6NNA9
, A9UL12, O75229, Q7LE08, Q9BTA6, Q9Y5A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 8, 2011
Last modified: June 24, 2015
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Antigen recognized by autologous antibody in patients with renal-cell carcinoma.

Caution

An ORF (C19orf029 OS) has been described in the opposite strand of the C-terminus of this gene.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.