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Q8WUQ7 (CATIN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cactin
Alternative name(s):
Renal carcinoma antigen NY-REN-24
Gene names
Name:CACTIN
Synonyms:C19orf29
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length758 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the regulation of innate immune response. Acts as negative regulator of Toll-like receptor and interferon-regulatory factor (IRF) signaling pathways. Contributes to the regulation of transcriptional activation of NF-kappa-B target genes in response to endogenous proinflammatory stimuli. May play a role during early embryonic development. Probably involved in pre-mRNA splicing. Ref.7

Subunit structure

Interacts (via N-terminal domain) with NFKBIL1; the interaction occurs in a proinflammatory-independent manner. Does not interact with RELA NF-kappa-B subunit. Identified in the spliceosome C complex. Ref.6 Ref.7

Subcellular location

Nucleus. Note: Nuclear localization with a speckled expression pattern in some cells. Colocalizes with NFKBIL1 in the nucleus. Ref.7

Miscellaneous

Antigen recognized by autologous antibody in patients with renal-cell carcinoma.

Sequence similarities

Belongs to the CACTIN family.

Caution

An ORF (C19orf029 OS) has been described in the opposite strand of the C-terminus of this gene.

Sequence caution

The sequence AAC24305.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAC32903.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAD42868.1 differs from that shown. Reason: Frameshift at position 711.

The sequence AAH04262.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence EAW69298.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processImmunity
Innate immunity
mRNA processing
mRNA splicing
   Cellular componentNucleus
Spliceosome
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to interleukin-1

Inferred from direct assay Ref.7. Source: UniProtKB

cellular response to lipopolysaccharide

Inferred from direct assay Ref.7. Source: UniProtKB

cellular response to tumor necrosis factor

Inferred from direct assay Ref.7. Source: UniProtKB

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

mRNA splicing, via spliceosome

Inferred by curator Ref.6. Source: UniProtKB

multicellular organismal development

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of NF-kappaB transcription factor activity

Inferred from direct assay Ref.7. Source: UniProtKB

negative regulation of interferon-beta production

Inferred from mutant phenotype Ref.7. Source: UniProtKB

negative regulation of interleukin-8 production

Inferred from direct assay Ref.7. Source: UniProtKB

negative regulation of lipopolysaccharide-mediated signaling pathway

Inferred from mutant phenotype Ref.7. Source: UniProtKB

negative regulation of protein phosphorylation

Inferred from mutant phenotype Ref.7. Source: UniProtKB

negative regulation of toll-like receptor signaling pathway

Inferred from direct assay Ref.7. Source: UniProtKB

negative regulation of tumor necrosis factor production

Inferred from direct assay Ref.7. Source: UniProtKB

negative regulation of type I interferon-mediated signaling pathway

Inferred from mutant phenotype Ref.7. Source: UniProtKB

   Cellular_componentcatalytic step 2 spliceosome

Inferred from direct assay Ref.6. Source: UniProtKB

cytoplasm

Inferred from direct assay. Source: HPA

nucleus

Inferred from direct assay PubMed 15302935Ref.7. Source: UniProtKB

   Molecular_functionpoly(A) RNA binding

Inferred from direct assay PubMed 22681889. Source: UniProtKB

protein binding

Inferred from physical interaction Ref.7. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WUQ7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Isoform 2 (identifier: Q8WUQ7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     758-758: R → RPGPCWRPIR...LAYWQDRMFF
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 758758Cactin
PRO_0000231617

Regions

Coiled coil135 – 18551 Potential
Coiled coil225 – 27753 Potential
Compositional bias101 – 1077Poly-Arg
Compositional bias129 – 1335Poly-Ala

Natural variations

Alternative sequence7581R → RPGPCWRPIRHCRRDPLWTP TLCRDWPPTHPVLAGGVHFP AAGIPPPGLLTGPWSMRPVT PSFAHIRTVAPSHSPFSGQE GRGPHGCHSPGRSGPAGRLV LQHPTGTSPTEAKRKVPPGP PEGHPTSPVTSPRPPTAPPR HPASSGNSSVCFSKKTCRWE KKSFVLMELAYWQDRMFF in isoform 2.
VSP_017856

Experimental info

Sequence conflict4991A → V in AAH19848. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 8, 2011. Version 3.
Checksum: F540F151EB96BEFB

FASTA75888,702
        10         20         30         40         50         60 
MGRDTRSRSR SAGRRGRRRQ SQSGSRSRSR SHGRRNRRRR EDEGRRRRRR RSRERRSDSE 

        70         80         90        100        110        120 
EERWQRSGMR SRSPPRPKWH SRDGSSQSDS GEEQSRGQWA RRRRRARSWS PSSSASSSAS 

       130        140        150        160        170        180 
PGRSQSPRAA AAALSQQQSL QERLRLREER KQQEELMKAF ETPEEKRARR LAKKEAKERK 

       190        200        210        220        230        240 
KREKMGWGEE YMGYTNTDNP FGDNNLLGTF IWNKALEKKG ISHLEEKELK ERNKRIQEDN 

       250        260        270        280        290        300 
RLELQKVKQL RLEREREKAM REQELEMLQR EKEAEHFKTW EEQEDNFHLQ QAKLRSKIRI 

       310        320        330        340        350        360 
RDGRAKPIDL LAKYISAEDD DLAVEMHEPY TFLNGLTVAD MEDLLEDIQV YMELEQGKNA 

       370        380        390        400        410        420 
DFWRDMTTIT EDEISKLRKL EASGKGPGER REGVNASVSS DVQSVFKGKT YNQLQVIFQG 

       430        440        450        460        470        480 
IEGKIRAGGP NLDMGYWESL LQQLRAHMAR ARLRERHQDV LRQKLYKLKQ EQGVESEPLF 

       490        500        510        520        530        540 
PILKQEPQSP SRSLEPEDAA PTPPGPSSEG GPAEAEVDGA TPTEGDGDGD GEGEGEGEAV 

       550        560        570        580        590        600 
LMEEDLIQQS LDDYDAGRYS PRLLTAHELP LDAHVLEPDE DLQRLQLSRQ QLQVTGDASE 

       610        620        630        640        650        660 
SAEDIFFRRA KEGMGQDEAQ FSVEMPLTGK AYLWADKYRP RKPRFFNRVH TGFEWNKYNQ 

       670        680        690        700        710        720 
THYDFDNPPP KIVQGYKFNI FYPDLIDKRS TPEYFLEACA DNKDFAILRF HAGPPYEDIA 

       730        740        750 
FKIVNREWEY SHRHGFRCQF ANGIFQLWFH FKRYRYRR 

« Hide

Isoform 2 [UniParc].

Checksum: E7238F52D6384E50
Show »

FASTA935107,938

References

« Hide 'large scale' references
[1]Cai Y., Yan Y.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 123-758.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 69-758.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 469-758 (ISOFORM 1).
Tissue: Cervix and Uterus.
[5]"Antigens recognized by autologous antibody in patients with renal-cell carcinoma."
Scanlan M.J., Gordan J.D., Williamson B., Stockert E., Bander N.H., Jongeneel C.V., Gure A.O., Jaeger D., Jaeger E., Knuth A., Chen Y.-T., Old L.J.
Int. J. Cancer 83:456-464(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 534-758 (ISOFORM 1), IDENTIFICATION AS A RENAL CANCER ANTIGEN.
Tissue: Renal cell carcinoma.
[6]"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.
RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE SPLICEOSOMAL C COMPLEX.
[7]"Cactin targets the MHC class III protein IkappaB-like (IkappaBL) and inhibits NF-kappaB and interferon-regulatory factor signaling pathways."
Atzei P., Gargan S., Curran N., Moynagh P.N.
J. Biol. Chem. 285:36804-36817(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH NFKBIL1, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY917150 mRNA. Translation: AAX84551.1.
AC005175 Genomic DNA. Translation: AAC24305.1. Sequence problems.
AC005542 Genomic DNA. Translation: AAC32903.1. Sequence problems.
AF155102 mRNA. Translation: AAD42868.1. Frameshift.
CH471139 Genomic DNA. Translation: EAW69298.1. Frameshift.
BC004262 mRNA. Translation: AAH04262.1. Different initiation.
BC019848 mRNA. Translation: AAH19848.1.
CCDSCCDS45920.1. [Q8WUQ7-1]
PIRT02672.
RefSeqNP_001074012.1. NM_001080543.1. [Q8WUQ7-1]
NP_067054.1. NM_021231.1. [Q8WUQ7-1]
UniGeneHs.128425.

3D structure databases

ProteinModelPortalQ8WUQ7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid121836. 3 interactions.
IntActQ8WUQ7. 2 interactions.
MINTMINT-4654969.
STRING9606.ENSP00000248420.

PTM databases

PhosphoSiteQ8WUQ7.

Polymorphism databases

DMDM322510133.

Proteomic databases

MaxQBQ8WUQ7.
PaxDbQ8WUQ7.
PRIDEQ8WUQ7.

Protocols and materials databases

DNASU58509.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000248420; ENSP00000248420; ENSG00000105298. [Q8WUQ7-1]
ENST00000429344; ENSP00000415078; ENSG00000105298. [Q8WUQ7-1]
ENST00000585942; ENSP00000465751; ENSG00000105298. [Q8WUQ7-1]
GeneID58509.
KEGGhsa:58509.
UCSCuc002lyh.3. human. [Q8WUQ7-1]

Organism-specific databases

CTD58509.
GeneCardsGC19M003610.
H-InvDBHIX0014643.
HIX0027556.
HGNCHGNC:29938. CACTIN.
HPAHPA042504.
HPA042548.
neXtProtNX_Q8WUQ7.
PharmGKBPA134932683.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG290728.
HOVERGENHBG079755.
InParanoidQ8WUQ7.
OrthoDBEOG7XM2Z3.
PhylomeDBQ8WUQ7.
TreeFamTF300906.

Gene expression databases

ArrayExpressQ8WUQ7.
BgeeQ8WUQ7.
CleanExHS_C19orf29.
GenevestigatorQ8WUQ7.

Family and domain databases

InterProIPR019134. Cactin_C.
IPR018816. Cactin_dom.
[Graphical view]
PfamPF10312. Cactin_mid. 1 hit.
PF09732. CactinC_cactus. 1 hit.
[Graphical view]
SMARTSM01050. CactinC_cactus. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiC19orf29.
GenomeRNAi58509.
NextBio65027.
PROQ8WUQ7.

Entry information

Entry nameCATIN_HUMAN
AccessionPrimary (citable) accession number: Q8WUQ7
Secondary accession number(s): A6NNA9 expand/collapse secondary AC list , A9UL12, O75229, Q7LE08, Q9BTA6, Q9Y5A4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 8, 2011
Last modified: July 9, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM