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Protein

Sodium-dependent phosphate transporter 1

Gene

SLC20A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor as it confers human cells susceptibility to infection to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B) as well as 10A1 murine leukemia virus (10A1 MLV).8 Publications

Kineticsi

With an increase in pH, a decrease in phosphate uptake is observed.

  1. KM=24.1 µM for phosphate2 Publications

Vmax=1.9 nmol/min/mg enzyme (in the presence of 0.05-2 mM phosphate)2 Publications

pH dependencei

Optimum pH is 6.5 and 7.5.2 Publications

GO - Molecular functioni

  1. high-affinity inorganic phosphate:sodium symporter activity Source: Ensembl
  2. inorganic phosphate transmembrane transporter activity Source: InterPro
  3. receptor activity Source: ProtInc
  4. signal transducer activity Source: UniProtKB
  5. sodium:phosphate symporter activity Source: ProtInc

GO - Biological processi

  1. ion transport Source: Reactome
  2. phosphate-containing compound metabolic process Source: ProtInc
  3. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  4. signal transduction Source: GOC
  5. transmembrane transport Source: Reactome
  6. transport Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Phosphate transport, Symport, Transport

Enzyme and pathway databases

BRENDAi3.6.3.27. 2681.
ReactomeiREACT_19275. Sodium-coupled phosphate cotransporters.

Protein family/group databases

TCDBi2.A.20.2.7. the inorganic phosphate transporter (pit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent phosphate transporter 1
Alternative name(s):
Gibbon ape leukemia virus receptor 1
Short name:
GLVR-1
Leukemia virus receptor 1 homolog
Phosphate transporter 1
Short name:
PiT-1
Solute carrier family 20 member 1
Gene namesi
Name:SLC20A1
Synonyms:GLVR1, PIT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:10946. SLC20A1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei21 – 4121HelicalSequence AnalysisAdd
BLAST
Topological domaini42 – 6120ExtracellularSequence AnalysisAdd
BLAST
Transmembranei62 – 8221HelicalSequence AnalysisAdd
BLAST
Topological domaini83 – 9917CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence AnalysisAdd
BLAST
Topological domaini121 – 15737ExtracellularSequence AnalysisAdd
BLAST
Transmembranei158 – 17821HelicalSequence AnalysisAdd
BLAST
Topological domaini179 – 20224CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei203 – 22321HelicalSequence AnalysisAdd
BLAST
Topological domaini224 – 2296ExtracellularSequence Analysis
Transmembranei230 – 25021HelicalSequence AnalysisAdd
BLAST
Topological domaini251 – 510260CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei511 – 53121HelicalSequence AnalysisAdd
BLAST
Topological domaini532 – 55726ExtracellularSequence AnalysisAdd
BLAST
Transmembranei558 – 57821HelicalSequence AnalysisAdd
BLAST
Topological domaini579 – 59921CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei600 – 62021HelicalSequence AnalysisAdd
BLAST
Topological domaini621 – 64929ExtracellularSequence AnalysisAdd
BLAST
Transmembranei650 – 67021HelicalSequence AnalysisAdd
BLAST
Topological domaini671 – 6799CytoplasmicSequence Analysis

GO - Cellular componenti

  1. integral component of plasma membrane Source: ProtInc
  2. membrane Source: ProtInc
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi550 – 5589DTGDVSSKV → KQEASTKA: Loss of virus infectibility. 1 Publication
Mutagenesisi550 – 5501D → K: Drastic reduction of virus infectibility, but conserved virus binding ability. 2 Publications
Mutagenesisi550 – 5501Missing : Loss of virus infectibility. 2 Publications

Organism-specific databases

PharmGKBiPA35833.

Polymorphism and mutation databases

BioMutaiSLC20A1.
DMDMi74730735.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 679679Sodium-dependent phosphate transporter 1PRO_0000080771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei265 – 2651PhosphoserineBy similarity
Modified residuei269 – 2691Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8WUM9.
PaxDbiQ8WUM9.
PRIDEiQ8WUM9.

PTM databases

PhosphoSiteiQ8WUM9.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Inductioni

By phosphate deprivation as well as by IL8/interleukin-8 in hypertrophic chondrocytes.2 Publications

Gene expression databases

BgeeiQ8WUM9.
CleanExiHS_SLC20A1.
ExpressionAtlasiQ8WUM9. baseline and differential.
GenevestigatoriQ8WUM9.

Organism-specific databases

HPAiCAB019279.
HPA035834.

Interactioni

Protein-protein interaction databases

BioGridi112462. 33 interactions.
IntActiQ8WUM9. 4 interactions.
STRINGi9606.ENSP00000272542.

Structurei

3D structure databases

ProteinModelPortaliQ8WUM9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni550 – 5589A

Domaini

Region A confers human cells susceptibility to infection by Gibbon Ape Leukemia Virus (GaLV) and Feline leukemia virus subgroup B (FeLV-B). Substitution of Human SLC20A1 region A by region A of murine SLC20A1 prevents viral infection.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0306.
GeneTreeiENSGT00390000014879.
HOGENOMiHOG000231892.
HOVERGENiHBG053358.
InParanoidiQ8WUM9.
KOiK14640.
OMAiWINLATW.
OrthoDBiEOG79KPDT.
PhylomeDBiQ8WUM9.
TreeFamiTF314426.

Family and domain databases

InterProiIPR001204. Phos_transporter.
[Graphical view]
PANTHERiPTHR11101. PTHR11101. 1 hit.
PfamiPF01384. PHO4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8WUM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLITSTTA ATAASGPLVD YLWMLILGFI IAFVLAFSVG ANDVANSFGT
60 70 80 90 100
AVGSGVVTLK QACILASIFE TVGSVLLGAK VSETIRKGLI DVEMYNSTQG
110 120 130 140 150
LLMAGSVSAM FGSAVWQLVA SFLKLPISGT HCIVGATIGF SLVAKGQEGV
160 170 180 190 200
KWSELIKIVM SWFVSPLLSG IMSGILFFLV RAFILHKADP VPNGLRALPV
210 220 230 240 250
FYACTVGINL FSIMYTGAPL LGFDKLPLWG TILISVGCAV FCALIVWFFV
260 270 280 290 300
CPRMKRKIER EIKCSPSESP LMEKKNSLKE DHEETKLSVG DIENKHPVSE
310 320 330 340 350
VGPATVPLQA VVEERTVSFK LGDLEEAPER ERLPSVDLKE ETSIDSTVNG
360 370 380 390 400
AVQLPNGNLV QFSQAVSNQI NSSGHYQYHT VHKDSGLYKE LLHKLHLAKV
410 420 430 440 450
GDCMGDSGDK PLRRNNSYTS YTMAICGMPL DSFRAKEGEQ KGEEMEKLTW
460 470 480 490 500
PNADSKKRIR MDSYTSYCNA VSDLHSASEI DMSVKAEMGL GDRKGSNGSL
510 520 530 540 550
EEWYDQDKPE VSLLFQFLQI LTACFGSFAH GGNDVSNAIG PLVALYLVYD
560 570 580 590 600
TGDVSSKVAT PIWLLLYGGV GICVGLWVWG RRVIQTMGKD LTPITPSSGF
610 620 630 640 650
SIELASALTV VIASNIGLPI STTHCKVGSV VSVGWLRSKK AVDWRLFRNI
660 670
FMAWFVTVPI SGVISAAIMA IFRYVILRM
Length:679
Mass (Da):73,700
Last modified:March 1, 2002 - v1
Checksum:i5545F6E6DC8F5EA8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti376 – 3761Y → S in AAA52572 (PubMed:2078500).Curated
Sequence conflicti443 – 4431E → R in AAD20286 (PubMed:9889306).Curated
Sequence conflicti487 – 4871E → A in AAA52572 (PubMed:2078500).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20859 mRNA. Translation: AAA52572.1.
AC079922 Genomic DNA. Translation: AAY14922.1.
BC019944 mRNA. Translation: AAH19944.1.
BC075818 mRNA. Translation: AAH75818.1.
AH007490 Genomic DNA. Translation: AAD20286.1.
CCDSiCCDS2099.1.
PIRiI52822.
RefSeqiNP_005406.3. NM_005415.4.
UniGeneiHs.187946.
Hs.732750.

Genome annotation databases

EnsembliENST00000272542; ENSP00000272542; ENSG00000144136.
GeneIDi6574.
KEGGihsa:6574.
UCSCiuc002tib.3. human.

Polymorphism and mutation databases

BioMutaiSLC20A1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20859 mRNA. Translation: AAA52572.1.
AC079922 Genomic DNA. Translation: AAY14922.1.
BC019944 mRNA. Translation: AAH19944.1.
BC075818 mRNA. Translation: AAH75818.1.
AH007490 Genomic DNA. Translation: AAD20286.1.
CCDSiCCDS2099.1.
PIRiI52822.
RefSeqiNP_005406.3. NM_005415.4.
UniGeneiHs.187946.
Hs.732750.

3D structure databases

ProteinModelPortaliQ8WUM9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112462. 33 interactions.
IntActiQ8WUM9. 4 interactions.
STRINGi9606.ENSP00000272542.

Protein family/group databases

TCDBi2.A.20.2.7. the inorganic phosphate transporter (pit) family.

PTM databases

PhosphoSiteiQ8WUM9.

Polymorphism and mutation databases

BioMutaiSLC20A1.
DMDMi74730735.

Proteomic databases

MaxQBiQ8WUM9.
PaxDbiQ8WUM9.
PRIDEiQ8WUM9.

Protocols and materials databases

DNASUi6574.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000272542; ENSP00000272542; ENSG00000144136.
GeneIDi6574.
KEGGihsa:6574.
UCSCiuc002tib.3. human.

Organism-specific databases

CTDi6574.
GeneCardsiGC02P113403.
HGNCiHGNC:10946. SLC20A1.
HPAiCAB019279.
HPA035834.
MIMi137570. gene.
neXtProtiNX_Q8WUM9.
PharmGKBiPA35833.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0306.
GeneTreeiENSGT00390000014879.
HOGENOMiHOG000231892.
HOVERGENiHBG053358.
InParanoidiQ8WUM9.
KOiK14640.
OMAiWINLATW.
OrthoDBiEOG79KPDT.
PhylomeDBiQ8WUM9.
TreeFamiTF314426.

Enzyme and pathway databases

BRENDAi3.6.3.27. 2681.
ReactomeiREACT_19275. Sodium-coupled phosphate cotransporters.

Miscellaneous databases

ChiTaRSiSLC20A1. human.
GeneWikiiSLC20A1.
GenomeRNAii6574.
NextBioi25579.
PROiQ8WUM9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8WUM9.
CleanExiHS_SLC20A1.
ExpressionAtlasiQ8WUM9. baseline and differential.
GenevestigatoriQ8WUM9.

Family and domain databases

InterProiIPR001204. Phos_transporter.
[Graphical view]
PANTHERiPTHR11101. PTHR11101. 1 hit.
PfamiPF01384. PHO4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus."
    O'Hara B., Johann S.V., Klinger H.P., Blair D.G., Rubinson H., Dunn K.J., Sass P., Vitek S.M., Robins T.
    Cell Growth Differ. 1:119-127(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney and Skin.
  4. "Characterization of the human Glvr-1 phosphate transporter/retrovirus receptor gene and promoter region."
    Palmer G., Manen D., Bonjour J.-P., Caverzasio J.
    Gene 226:25-33(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-646.
  5. "Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus."
    Takeuchi Y., Vile R.G., Simpson G., O'Hara B., Collins M.K., Weiss R.A.
    J. Virol. 66:1219-1222(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "GLVR1, a receptor for gibbon ape leukemia virus, is homologous to a phosphate permease of Neurospora crassa and is expressed at high levels in the brain and thymus."
    Johann S.V., Gibbons J.J., O'Hara B.
    J. Virol. 66:1635-1640(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TOPOLOGY.
  7. "Definition of a domain of GLVR1 which is necessary for infection by gibbon ape leukemia virus and which is highly polymorphic between species."
    Johann S.V., van Zeijl M., Cekleniak J., O'Hara B.
    J. Virol. 67:6733-6736(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF 550-ASP--VAL-558 AND ASP-550, REGION.
  8. "The cellular receptor for gibbon ape leukemia virus is a novel high affinity sodium-dependent phosphate transporter."
    Olah Z., Lehel C., Anderson W.B., Eiden M.V., Wilson C.A.
    J. Biol. Chem. 269:25426-25431(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  9. "A family of retroviruses that utilize related phosphate transporters for cell entry."
    Miller D.G., Miller A.D.
    J. Virol. 68:8270-8276(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters."
    Kavanaugh M.P., Miller D.G., Zhang W., Law W., Kozak S.L., Kabat D., Miller A.D.
    Proc. Natl. Acad. Sci. U.S.A. 91:7071-7075(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
  11. "Phosphate regulation of vascular smooth muscle cell calcification."
    Jono S., McKee M.D., Murry C.E., Shioi A., Nishizawa Y., Mori K., Morii H., Giachelli C.M.
    Circ. Res. 87:E10-E17(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "Reassessing the role of region A in Pit1-mediated viral entry."
    Farrell K.B., Russ J.L., Murthy R.K., Eiden M.V.
    J. Virol. 76:7683-7693(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-550, REGION.
  13. "Role of interleukin-8 in PiT-1 expression and CXCR1-mediated inorganic phosphate uptake in chondrocytes."
    Cecil D.L., Rose D.M., Terkeltaub R., Liu-Bryan R.
    Arthritis Rheum. 52:144-154(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  14. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  15. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiS20A1_HUMAN
AccessioniPrimary (citable) accession number: Q8WUM9
Secondary accession number(s): Q08344, Q6DHX8, Q9UQ82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: April 29, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.