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Q8WUM9 (S20A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium-dependent phosphate transporter 1
Alternative name(s):
Gibbon ape leukemia virus receptor 1
Short name=GLVR-1
Leukemia virus receptor 1 homolog
Phosphate transporter 1
Short name=PiT-1
Solute carrier family 20 member 1
Gene names
Name:SLC20A1
Synonyms:GLVR1, PIT1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length679 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor as it confers human cells susceptibility to infection to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B) as well as 10A1 murine leukemia virus (10A1 MLV). Ref.1 Ref.5 Ref.6 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Ubiquitously expressed. Ref.1

Induction

By phosphate deprivation as well as by IL8/interleukin-8 in hypertrophic chondrocytes. Ref.10 Ref.13

Domain

Region A confers human cells susceptibility to infection by Gibbon Ape Leukemia Virus (GaLV) and Feline leukemia virus subgroup B (FeLV-B). Substitution of Human SLC20A1 region A by region A of murine SLC20A1 prevents viral infection.

Sequence similarities

Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

With an increase in pH, a decrease in phosphate uptake is observed.

KM=24.1 µM for phosphate Ref.8 Ref.10

Vmax=1.9 nmol/min/mg enzyme (in the presence of 0.05-2 mM phosphate)

pH dependence:

Optimum pH is 6.5 and 7.5.

Ontologies

Keywords
   Biological processPhosphate transport
Symport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_procession transport

Traceable author statement. Source: Reactome

phosphate ion transmembrane transport

Traceable author statement Ref.10. Source: GOC

phosphate-containing compound metabolic process

Traceable author statement Ref.10. Source: ProtInc

positive regulation of I-kappaB kinase/NF-kappaB signaling

Inferred from mutant phenotype PubMed 12761501. Source: UniProtKB

signal transduction

Inferred from mutant phenotype PubMed 12761501. Source: GOC

sodium ion transmembrane transport

Traceable author statement Ref.10. Source: GOC

transmembrane transport

Traceable author statement. Source: Reactome

transport

Traceable author statement Ref.10. Source: ProtInc

   Cellular_componentintegral component of plasma membrane

Traceable author statement Ref.10. Source: ProtInc

membrane

Traceable author statement Ref.10. Source: ProtInc

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionhigh affinity inorganic phosphate:sodium symporter activity

Inferred from electronic annotation. Source: Ensembl

inorganic phosphate transmembrane transporter activity

Inferred from electronic annotation. Source: InterPro

receptor activity

Traceable author statement Ref.10. Source: ProtInc

signal transducer activity

Inferred from mutant phenotype PubMed 12761501. Source: UniProtKB

sodium:phosphate symporter activity

Traceable author statement Ref.10. Source: ProtInc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 679679Sodium-dependent phosphate transporter 1
PRO_0000080771

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4121Helical; Potential
Topological domain42 – 6120Extracellular Potential
Transmembrane62 – 8221Helical; Potential
Topological domain83 – 9917Cytoplasmic Potential
Transmembrane100 – 12021Helical; Potential
Topological domain121 – 15737Extracellular Potential
Transmembrane158 – 17821Helical; Potential
Topological domain179 – 20224Cytoplasmic Potential
Transmembrane203 – 22321Helical; Potential
Topological domain224 – 2296Extracellular Potential
Transmembrane230 – 25021Helical; Potential
Topological domain251 – 510260Cytoplasmic Potential
Transmembrane511 – 53121Helical; Potential
Topological domain532 – 55726Extracellular Potential
Transmembrane558 – 57821Helical; Potential
Topological domain579 – 59921Cytoplasmic Potential
Transmembrane600 – 62021Helical; Potential
Topological domain621 – 64929Extracellular Potential
Transmembrane650 – 67021Helical; Potential
Topological domain671 – 6799Cytoplasmic Potential
Region550 – 5589A

Amino acid modifications

Modified residue2651Phosphoserine By similarity
Modified residue2691Phosphoserine Ref.14

Experimental info

Mutagenesis550 – 5589DTGDVSSKV → KQEASTKA: Loss of virus infectibility. Ref.7 Ref.12
Mutagenesis5501D → K: Drastic reduction of virus infectibility, but conserved virus binding ability. Ref.7 Ref.12
Mutagenesis5501Missing: Loss of virus infectibility. Ref.7 Ref.12
Sequence conflict3761Y → S in AAA52572. Ref.1
Sequence conflict4431E → R in AAD20286. Ref.4
Sequence conflict4871E → A in AAA52572. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8WUM9 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 5545F6E6DC8F5EA8

FASTA67973,700
        10         20         30         40         50         60 
MATLITSTTA ATAASGPLVD YLWMLILGFI IAFVLAFSVG ANDVANSFGT AVGSGVVTLK 

        70         80         90        100        110        120 
QACILASIFE TVGSVLLGAK VSETIRKGLI DVEMYNSTQG LLMAGSVSAM FGSAVWQLVA 

       130        140        150        160        170        180 
SFLKLPISGT HCIVGATIGF SLVAKGQEGV KWSELIKIVM SWFVSPLLSG IMSGILFFLV 

       190        200        210        220        230        240 
RAFILHKADP VPNGLRALPV FYACTVGINL FSIMYTGAPL LGFDKLPLWG TILISVGCAV 

       250        260        270        280        290        300 
FCALIVWFFV CPRMKRKIER EIKCSPSESP LMEKKNSLKE DHEETKLSVG DIENKHPVSE 

       310        320        330        340        350        360 
VGPATVPLQA VVEERTVSFK LGDLEEAPER ERLPSVDLKE ETSIDSTVNG AVQLPNGNLV 

       370        380        390        400        410        420 
QFSQAVSNQI NSSGHYQYHT VHKDSGLYKE LLHKLHLAKV GDCMGDSGDK PLRRNNSYTS 

       430        440        450        460        470        480 
YTMAICGMPL DSFRAKEGEQ KGEEMEKLTW PNADSKKRIR MDSYTSYCNA VSDLHSASEI 

       490        500        510        520        530        540 
DMSVKAEMGL GDRKGSNGSL EEWYDQDKPE VSLLFQFLQI LTACFGSFAH GGNDVSNAIG 

       550        560        570        580        590        600 
PLVALYLVYD TGDVSSKVAT PIWLLLYGGV GICVGLWVWG RRVIQTMGKD LTPITPSSGF 

       610        620        630        640        650        660 
SIELASALTV VIASNIGLPI STTHCKVGSV VSVGWLRSKK AVDWRLFRNI FMAWFVTVPI 

       670 
SGVISAAIMA IFRYVILRM 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus."
O'Hara B., Johann S.V., Klinger H.P., Blair D.G., Rubinson H., Dunn K.J., Sass P., Vitek S.M., Robins T.
Cell Growth Differ. 1:119-127(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney and Skin.
[4]"Characterization of the human Glvr-1 phosphate transporter/retrovirus receptor gene and promoter region."
Palmer G., Manen D., Bonjour J.-P., Caverzasio J.
Gene 226:25-33(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-646.
[5]"Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus."
Takeuchi Y., Vile R.G., Simpson G., O'Hara B., Collins M.K., Weiss R.A.
J. Virol. 66:1219-1222(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"GLVR1, a receptor for gibbon ape leukemia virus, is homologous to a phosphate permease of Neurospora crassa and is expressed at high levels in the brain and thymus."
Johann S.V., Gibbons J.J., O'Hara B.
J. Virol. 66:1635-1640(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TOPOLOGY.
[7]"Definition of a domain of GLVR1 which is necessary for infection by gibbon ape leukemia virus and which is highly polymorphic between species."
Johann S.V., van Zeijl M., Cekleniak J., O'Hara B.
J. Virol. 67:6733-6736(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF 550-ASP--VAL-558 AND ASP-550, REGION.
[8]"The cellular receptor for gibbon ape leukemia virus is a novel high affinity sodium-dependent phosphate transporter."
Olah Z., Lehel C., Anderson W.B., Eiden M.V., Wilson C.A.
J. Biol. Chem. 269:25426-25431(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[9]"A family of retroviruses that utilize related phosphate transporters for cell entry."
Miller D.G., Miller A.D.
J. Virol. 68:8270-8276(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters."
Kavanaugh M.P., Miller D.G., Zhang W., Law W., Kozak S.L., Kabat D., Miller A.D.
Proc. Natl. Acad. Sci. U.S.A. 91:7071-7075(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
[11]"Phosphate regulation of vascular smooth muscle cell calcification."
Jono S., McKee M.D., Murry C.E., Shioi A., Nishizawa Y., Mori K., Morii H., Giachelli C.M.
Circ. Res. 87:E10-E17(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Reassessing the role of region A in Pit1-mediated viral entry."
Farrell K.B., Russ J.L., Murthy R.K., Eiden M.V.
J. Virol. 76:7683-7693(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF ASP-550, REGION.
[13]"Role of interleukin-8 in PiT-1 expression and CXCR1-mediated inorganic phosphate uptake in chondrocytes."
Cecil D.L., Rose D.M., Terkeltaub R., Liu-Bryan R.
Arthritis Rheum. 52:144-154(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[14]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[15]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L20859 mRNA. Translation: AAA52572.1.
AC079922 Genomic DNA. Translation: AAY14922.1.
BC019944 mRNA. Translation: AAH19944.1.
BC075818 mRNA. Translation: AAH75818.1.
AH007490 Genomic DNA. Translation: AAD20286.1.
CCDSCCDS2099.1.
PIRI52822.
RefSeqNP_005406.3. NM_005415.4.
UniGeneHs.187946.
Hs.732750.

3D structure databases

ProteinModelPortalQ8WUM9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112462. 5 interactions.
IntActQ8WUM9. 4 interactions.
STRING9606.ENSP00000272542.

Protein family/group databases

TCDB2.A.20.2.7. the inorganic phosphate transporter (pit) family.

PTM databases

PhosphoSiteQ8WUM9.

Polymorphism databases

DMDM74730735.

Proteomic databases

MaxQBQ8WUM9.
PaxDbQ8WUM9.
PRIDEQ8WUM9.

Protocols and materials databases

DNASU6574.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000272542; ENSP00000272542; ENSG00000144136.
GeneID6574.
KEGGhsa:6574.
UCSCuc002tib.3. human.

Organism-specific databases

CTD6574.
GeneCardsGC02P113403.
HGNCHGNC:10946. SLC20A1.
HPACAB019279.
MIM137570. gene.
neXtProtNX_Q8WUM9.
PharmGKBPA35833.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0306.
HOGENOMHOG000231892.
HOVERGENHBG053358.
InParanoidQ8WUM9.
KOK14640.
OMASSLEEWC.
OrthoDBEOG79KPDT.
PhylomeDBQ8WUM9.
TreeFamTF314426.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressQ8WUM9.
BgeeQ8WUM9.
CleanExHS_SLC20A1.
GenevestigatorQ8WUM9.

Family and domain databases

InterProIPR001204. Phos_transporter.
[Graphical view]
PANTHERPTHR11101. PTHR11101. 1 hit.
PfamPF01384. PHO4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC20A1. human.
GeneWikiSLC20A1.
GenomeRNAi6574.
NextBio25579.
PROQ8WUM9.
SOURCESearch...

Entry information

Entry nameS20A1_HUMAN
AccessionPrimary (citable) accession number: Q8WUM9
Secondary accession number(s): Q08344, Q6DHX8, Q9UQ82
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM