Q8WUM9 (S20A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium-dependent phosphate transporter 1

Alternative name(s):
Gibbon ape leukemia virus receptor 1
Short name=GLVR-1
Leukemia virus receptor 1 homolog
Phosphate transporter 1
Short name=PiT-1
Solute carrier family 20 member 1

Gene names

Name:	SLC20A1
Synonyms:	GLVR1, PIT1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	679 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor as it confers human cells susceptibility to infection to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B) as well as 10A1 murine leukemia virus (10A1 MLV). Ref.1 Ref.5 Ref.6 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitously expressed. Ref.1
Induction	By phosphate deprivation as well as by IL8/interleukin-8 in hypertrophic chondrocytes. Ref.10 Ref.13
Domain	Region A confers human cells susceptibility to infection by Gibbon Ape Leukemia Virus (GaLV) and Feline leukemia virus subgroup B (FeLV-B). Substitution of Human SLC20A1 region A by region A of murine SLC20A1 prevents viral infection.
Sequence similarities	Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. [View classification]
Biophysicochemical properties	Kinetic parameters: With an increase in pH, a decrease in phosphate uptake is observed. KM=24.1 µM for phosphate Ref.8 Ref.10 Vmax=1.9 nmol/min/mg enzyme (in the presence of 0.05-2 mM phosphate) pH dependence: Optimum pH is 6.5 and 7.5.

Ontologies

Keywords
Biological process	Phosphate transport Symport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphate-containing compound metabolic process Traceable author statement Ref.10. Source: ProtInc positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from mutant phenotype PubMed 12761501. Source: UniProtKB
Cellular_component	integral to plasma membrane Traceable author statement Ref.10. Source: ProtInc
Molecular_function	high affinity inorganic phosphate:sodium symporter activity Inferred from electronic annotation. Source: Compara inorganic phosphate transmembrane transporter activity Inferred from electronic annotation. Source: InterPro receptor activity Traceable author statement Ref.10. Source: ProtInc signal transducer activity Inferred from mutant phenotype PubMed 12761501. Source: UniProtKB sodium:phosphate symporter activity Traceable author statement Ref.10. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 679	679	Sodium-dependent phosphate transporter 1		PRO_0000080771
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Helical; Potential
	Topological domain	42 – 61	20	Extracellular Potential
	Transmembrane	62 – 82	21	Helical; Potential
	Topological domain	83 – 99	17	Cytoplasmic Potential
	Transmembrane	100 – 120	21	Helical; Potential
	Topological domain	121 – 157	37	Extracellular Potential
	Transmembrane	158 – 178	21	Helical; Potential
	Topological domain	179 – 202	24	Cytoplasmic Potential
	Transmembrane	203 – 223	21	Helical; Potential
	Topological domain	224 – 229	6	Extracellular Potential
	Transmembrane	230 – 250	21	Helical; Potential
	Topological domain	251 – 510	260	Cytoplasmic Potential
	Transmembrane	511 – 531	21	Helical; Potential
	Topological domain	532 – 557	26	Extracellular Potential
	Transmembrane	558 – 578	21	Helical; Potential
	Topological domain	579 – 599	21	Cytoplasmic Potential
	Transmembrane	600 – 620	21	Helical; Potential
	Topological domain	621 – 649	29	Extracellular Potential
	Transmembrane	650 – 670	21	Helical; Potential
	Topological domain	671 – 679	9	Cytoplasmic Potential
	Region	550 – 558	9	A
Amino acid modifications
	Modified residue	265	1	Phosphoserine By similarity
	Modified residue	269	1	Phosphoserine Ref.14
Experimental info
	Mutagenesis	550 – 558	9	DTGDVSSKV → KQEASTKA: Loss of virus infectibility. Ref.7 Ref.12
	Mutagenesis	550	1	D → K: Drastic reduction of virus infectibility, but conserved virus binding ability. Ref.7 Ref.12
	Mutagenesis	550	1	Missing: Loss of virus infectibility. Ref.7 Ref.12
	Sequence conflict	376	1	Y → S in AAA52572. Ref.1
	Sequence conflict	443	1	E → R in AAD20286. Ref.4
	Sequence conflict	487	1	E → A in AAA52572. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8WUM9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 5545F6E6DC8F5EA8	FASTA	679	73,700
	10 20 30 40 50 60 MATLITSTTA ATAASGPLVD YLWMLILGFI IAFVLAFSVG ANDVANSFGT AVGSGVVTLK 70 80 90 100 110 120 QACILASIFE TVGSVLLGAK VSETIRKGLI DVEMYNSTQG LLMAGSVSAM FGSAVWQLVA 130 140 150 160 170 180 SFLKLPISGT HCIVGATIGF SLVAKGQEGV KWSELIKIVM SWFVSPLLSG IMSGILFFLV 190 200 210 220 230 240 RAFILHKADP VPNGLRALPV FYACTVGINL FSIMYTGAPL LGFDKLPLWG TILISVGCAV 250 260 270 280 290 300 FCALIVWFFV CPRMKRKIER EIKCSPSESP LMEKKNSLKE DHEETKLSVG DIENKHPVSE 310 320 330 340 350 360 VGPATVPLQA VVEERTVSFK LGDLEEAPER ERLPSVDLKE ETSIDSTVNG AVQLPNGNLV 370 380 390 400 410 420 QFSQAVSNQI NSSGHYQYHT VHKDSGLYKE LLHKLHLAKV GDCMGDSGDK PLRRNNSYTS 430 440 450 460 470 480 YTMAICGMPL DSFRAKEGEQ KGEEMEKLTW PNADSKKRIR MDSYTSYCNA VSDLHSASEI 490 500 510 520 530 540 DMSVKAEMGL GDRKGSNGSL EEWYDQDKPE VSLLFQFLQI LTACFGSFAH GGNDVSNAIG 550 560 570 580 590 600 PLVALYLVYD TGDVSSKVAT PIWLLLYGGV GICVGLWVWG RRVIQTMGKD LTPITPSSGF 610 620 630 640 650 660 SIELASALTV VIASNIGLPI STTHCKVGSV VSVGWLRSKK AVDWRLFRNI FMAWFVTVPI 670 SGVISAAIMA IFRYVILRM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus." O'Hara B., Johann S.V., Klinger H.P., Blair D.G., Rubinson H., Dunn K.J., Sass P., Vitek S.M., Robins T. Cell Growth Differ. 1:119-127(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
[2]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney and Skin.
[4]	"Characterization of the human Glvr-1 phosphate transporter/retrovirus receptor gene and promoter region." Palmer G., Manen D., Bonjour J.-P., Caverzasio J. Gene 226:25-33(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-646.
[5]	"Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus." Takeuchi Y., Vile R.G., Simpson G., O'Hara B., Collins M.K., Weiss R.A. J. Virol. 66:1219-1222(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"GLVR1, a receptor for gibbon ape leukemia virus, is homologous to a phosphate permease of Neurospora crassa and is expressed at high levels in the brain and thymus." Johann S.V., Gibbons J.J., O'Hara B. J. Virol. 66:1635-1640(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TOPOLOGY.
[7]	"Definition of a domain of GLVR1 which is necessary for infection by gibbon ape leukemia virus and which is highly polymorphic between species." Johann S.V., van Zeijl M., Cekleniak J., O'Hara B. J. Virol. 67:6733-6736(1993) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF 550-ASP--VAL-558 AND ASP-550, REGION.
[8]	"The cellular receptor for gibbon ape leukemia virus is a novel high affinity sodium-dependent phosphate transporter." Olah Z., Lehel C., Anderson W.B., Eiden M.V., Wilson C.A. J. Biol. Chem. 269:25426-25431(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[9]	"A family of retroviruses that utilize related phosphate transporters for cell entry." Miller D.G., Miller A.D. J. Virol. 68:8270-8276(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters." Kavanaugh M.P., Miller D.G., Zhang W., Law W., Kozak S.L., Kabat D., Miller A.D. Proc. Natl. Acad. Sci. U.S.A. 91:7071-7075(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
[11]	"Phosphate regulation of vascular smooth muscle cell calcification." Jono S., McKee M.D., Murry C.E., Shioi A., Nishizawa Y., Mori K., Morii H., Giachelli C.M. Circ. Res. 87:E10-E17(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Reassessing the role of region A in Pit1-mediated viral entry." Farrell K.B., Russ J.L., Murthy R.K., Eiden M.V. J. Virol. 76:7683-7693(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-550, REGION.
[13]	"Role of interleukin-8 in PiT-1 expression and CXCR1-mediated inorganic phosphate uptake in chondrocytes." Cecil D.L., Rose D.M., Terkeltaub R., Liu-Bryan R. Arthritis Rheum. 52:144-154(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[14]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L20859 mRNA. Translation: AAA52572.1. AC079922 Genomic DNA. Translation: AAY14922.1. BC019944 mRNA. Translation: AAH19944.1. BC075818 mRNA. Translation: AAH75818.1. AH007490 Genomic DNA. Translation: AAD20286.1.
IPI	IPI00023035.
PIR	I52822.
RefSeq	NP_005406.3. NM_005415.4.
UniGene	Hs.187946. Hs.732750.
3D structure databases
ProteinModelPortal	Q8WUM9.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8WUM9. 3 interactions.
STRING	9606.ENSP00000272542.
PTM databases
PhosphoSite	Q8WUM9.
Polymorphism databases
DMDM	74730735.
Proteomic databases
PaxDb	Q8WUM9.
PRIDE	Q8WUM9.
Protocols and materials databases
DNASU	6574.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000272542; ENSP00000272542; ENSG00000144136.
GeneID	6574.
KEGG	hsa:6574.
UCSC	uc002tib.3. human.
Organism-specific databases
CTD	6574.
GeneCards	GC02P113403.
HGNC	HGNC:10946. SLC20A1.
HPA	CAB019279.
MIM	137570. gene.
neXtProt	NX_Q8WUM9.
PharmGKB	PA35833.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0306.
HOGENOM	HOG000231892.
HOVERGEN	HBG053358.
InParanoid	Q8WUM9.
KO	K14640.
OMA	SIDSAMN.
OrthoDB	EOG428212.
PhylomeDB	Q8WUM9.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	Q8WUM9.
Bgee	Q8WUM9.
CleanEx	HS_SLC20A1.
Genevestigator	Q8WUM9.
GermOnline	ENSG00000144136. Homo sapiens.
Family and domain databases
InterPro	IPR001204. Phos_transporter. [Graphical view]
PANTHER	PTHR11101. PTHR11101. 1 hit.
Pfam	PF01384. PHO4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC20A1. human.
GenomeRNAi	6574.
NextBio	25579.
SOURCE	Search...

Entry information

Entry name

S20A1_HUMAN

Accession

Primary (citable) accession number: Q8WUM9
Secondary accession number(s): Q08344, Q6DHX8, Q9UQ82

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.