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Q8WUM9

- S20A1_HUMAN

UniProt

Q8WUM9 - S20A1_HUMAN

Protein

Sodium-dependent phosphate transporter 1

Gene

SLC20A1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
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    Functioni

    Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification. May function as a retroviral receptor as it confers human cells susceptibility to infection to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B) as well as 10A1 murine leukemia virus (10A1 MLV).8 Publications

    Kineticsi

    With an increase in pH, a decrease in phosphate uptake is observed.

    1. KM=24.1 µM for phosphate2 Publications

    Vmax=1.9 nmol/min/mg enzyme (in the presence of 0.05-2 mM phosphate)2 Publications

    pH dependencei

    Optimum pH is 6.5 and 7.5.2 Publications

    GO - Molecular functioni

    1. high-affinity inorganic phosphate:sodium symporter activity Source: Ensembl
    2. inorganic phosphate transmembrane transporter activity Source: InterPro
    3. receptor activity Source: ProtInc
    4. signal transducer activity Source: UniProtKB
    5. sodium:phosphate symporter activity Source: ProtInc

    GO - Biological processi

    1. ion transport Source: Reactome
    2. phosphate-containing compound metabolic process Source: ProtInc
    3. phosphate ion transmembrane transport Source: GOC
    4. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
    5. signal transduction Source: GOC
    6. sodium ion transmembrane transport Source: GOC
    7. transmembrane transport Source: Reactome
    8. transport Source: ProtInc

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Phosphate transport, Symport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_19275. Sodium-coupled phosphate cotransporters.

    Protein family/group databases

    TCDBi2.A.20.2.7. the inorganic phosphate transporter (pit) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium-dependent phosphate transporter 1
    Alternative name(s):
    Gibbon ape leukemia virus receptor 1
    Short name:
    GLVR-1
    Leukemia virus receptor 1 homolog
    Phosphate transporter 1
    Short name:
    PiT-1
    Solute carrier family 20 member 1
    Gene namesi
    Name:SLC20A1
    Synonyms:GLVR1, PIT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:10946. SLC20A1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: ProtInc
    2. membrane Source: ProtInc
    3. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi550 – 5589DTGDVSSKV → KQEASTKA: Loss of virus infectibility. 2 Publications
    Mutagenesisi550 – 5501D → K: Drastic reduction of virus infectibility, but conserved virus binding ability. 2 Publications
    Mutagenesisi550 – 5501Missing: Loss of virus infectibility. 2 Publications

    Organism-specific databases

    PharmGKBiPA35833.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 679679Sodium-dependent phosphate transporter 1PRO_0000080771Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei265 – 2651PhosphoserineBy similarity
    Modified residuei269 – 2691Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8WUM9.
    PaxDbiQ8WUM9.
    PRIDEiQ8WUM9.

    PTM databases

    PhosphoSiteiQ8WUM9.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Inductioni

    By phosphate deprivation as well as by IL8/interleukin-8 in hypertrophic chondrocytes.2 Publications

    Gene expression databases

    ArrayExpressiQ8WUM9.
    BgeeiQ8WUM9.
    CleanExiHS_SLC20A1.
    GenevestigatoriQ8WUM9.

    Organism-specific databases

    HPAiCAB019279.

    Interactioni

    Protein-protein interaction databases

    BioGridi112462. 5 interactions.
    IntActiQ8WUM9. 4 interactions.
    STRINGi9606.ENSP00000272542.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8WUM9.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini42 – 6120ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini83 – 9917CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini121 – 15737ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini179 – 20224CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini224 – 2296ExtracellularSequence Analysis
    Topological domaini251 – 510260CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini532 – 55726ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini579 – 59921CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini621 – 64929ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini671 – 6799CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei62 – 8221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei100 – 12021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei158 – 17821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei203 – 22321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei230 – 25021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei511 – 53121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei558 – 57821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei600 – 62021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei650 – 67021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni550 – 5589A

    Domaini

    Region A confers human cells susceptibility to infection by Gibbon Ape Leukemia Virus (GaLV) and Feline leukemia virus subgroup B (FeLV-B). Substitution of Human SLC20A1 region A by region A of murine SLC20A1 prevents viral infection.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0306.
    HOGENOMiHOG000231892.
    HOVERGENiHBG053358.
    InParanoidiQ8WUM9.
    KOiK14640.
    OMAiSSLEEWC.
    OrthoDBiEOG79KPDT.
    PhylomeDBiQ8WUM9.
    TreeFamiTF314426.

    Family and domain databases

    InterProiIPR001204. Phos_transporter.
    [Graphical view]
    PANTHERiPTHR11101. PTHR11101. 1 hit.
    PfamiPF01384. PHO4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8WUM9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATLITSTTA ATAASGPLVD YLWMLILGFI IAFVLAFSVG ANDVANSFGT    50
    AVGSGVVTLK QACILASIFE TVGSVLLGAK VSETIRKGLI DVEMYNSTQG 100
    LLMAGSVSAM FGSAVWQLVA SFLKLPISGT HCIVGATIGF SLVAKGQEGV 150
    KWSELIKIVM SWFVSPLLSG IMSGILFFLV RAFILHKADP VPNGLRALPV 200
    FYACTVGINL FSIMYTGAPL LGFDKLPLWG TILISVGCAV FCALIVWFFV 250
    CPRMKRKIER EIKCSPSESP LMEKKNSLKE DHEETKLSVG DIENKHPVSE 300
    VGPATVPLQA VVEERTVSFK LGDLEEAPER ERLPSVDLKE ETSIDSTVNG 350
    AVQLPNGNLV QFSQAVSNQI NSSGHYQYHT VHKDSGLYKE LLHKLHLAKV 400
    GDCMGDSGDK PLRRNNSYTS YTMAICGMPL DSFRAKEGEQ KGEEMEKLTW 450
    PNADSKKRIR MDSYTSYCNA VSDLHSASEI DMSVKAEMGL GDRKGSNGSL 500
    EEWYDQDKPE VSLLFQFLQI LTACFGSFAH GGNDVSNAIG PLVALYLVYD 550
    TGDVSSKVAT PIWLLLYGGV GICVGLWVWG RRVIQTMGKD LTPITPSSGF 600
    SIELASALTV VIASNIGLPI STTHCKVGSV VSVGWLRSKK AVDWRLFRNI 650
    FMAWFVTVPI SGVISAAIMA IFRYVILRM 679
    Length:679
    Mass (Da):73,700
    Last modified:March 1, 2002 - v1
    Checksum:i5545F6E6DC8F5EA8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti376 – 3761Y → S in AAA52572. (PubMed:2078500)Curated
    Sequence conflicti443 – 4431E → R in AAD20286. (PubMed:9889306)Curated
    Sequence conflicti487 – 4871E → A in AAA52572. (PubMed:2078500)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20859 mRNA. Translation: AAA52572.1.
    AC079922 Genomic DNA. Translation: AAY14922.1.
    BC019944 mRNA. Translation: AAH19944.1.
    BC075818 mRNA. Translation: AAH75818.1.
    AH007490 Genomic DNA. Translation: AAD20286.1.
    CCDSiCCDS2099.1.
    PIRiI52822.
    RefSeqiNP_005406.3. NM_005415.4.
    UniGeneiHs.187946.
    Hs.732750.

    Genome annotation databases

    EnsembliENST00000272542; ENSP00000272542; ENSG00000144136.
    GeneIDi6574.
    KEGGihsa:6574.
    UCSCiuc002tib.3. human.

    Polymorphism databases

    DMDMi74730735.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L20859 mRNA. Translation: AAA52572.1 .
    AC079922 Genomic DNA. Translation: AAY14922.1 .
    BC019944 mRNA. Translation: AAH19944.1 .
    BC075818 mRNA. Translation: AAH75818.1 .
    AH007490 Genomic DNA. Translation: AAD20286.1 .
    CCDSi CCDS2099.1.
    PIRi I52822.
    RefSeqi NP_005406.3. NM_005415.4.
    UniGenei Hs.187946.
    Hs.732750.

    3D structure databases

    ProteinModelPortali Q8WUM9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112462. 5 interactions.
    IntActi Q8WUM9. 4 interactions.
    STRINGi 9606.ENSP00000272542.

    Protein family/group databases

    TCDBi 2.A.20.2.7. the inorganic phosphate transporter (pit) family.

    PTM databases

    PhosphoSitei Q8WUM9.

    Polymorphism databases

    DMDMi 74730735.

    Proteomic databases

    MaxQBi Q8WUM9.
    PaxDbi Q8WUM9.
    PRIDEi Q8WUM9.

    Protocols and materials databases

    DNASUi 6574.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000272542 ; ENSP00000272542 ; ENSG00000144136 .
    GeneIDi 6574.
    KEGGi hsa:6574.
    UCSCi uc002tib.3. human.

    Organism-specific databases

    CTDi 6574.
    GeneCardsi GC02P113403.
    HGNCi HGNC:10946. SLC20A1.
    HPAi CAB019279.
    MIMi 137570. gene.
    neXtProti NX_Q8WUM9.
    PharmGKBi PA35833.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0306.
    HOGENOMi HOG000231892.
    HOVERGENi HBG053358.
    InParanoidi Q8WUM9.
    KOi K14640.
    OMAi SSLEEWC.
    OrthoDBi EOG79KPDT.
    PhylomeDBi Q8WUM9.
    TreeFami TF314426.

    Enzyme and pathway databases

    Reactomei REACT_19275. Sodium-coupled phosphate cotransporters.

    Miscellaneous databases

    ChiTaRSi SLC20A1. human.
    GeneWikii SLC20A1.
    GenomeRNAii 6574.
    NextBioi 25579.
    PROi Q8WUM9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8WUM9.
    Bgeei Q8WUM9.
    CleanExi HS_SLC20A1.
    Genevestigatori Q8WUM9.

    Family and domain databases

    InterProi IPR001204. Phos_transporter.
    [Graphical view ]
    PANTHERi PTHR11101. PTHR11101. 1 hit.
    Pfami PF01384. PHO4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a human gene conferring sensitivity to infection by gibbon ape leukemia virus."
      O'Hara B., Johann S.V., Klinger H.P., Blair D.G., Rubinson H., Dunn K.J., Sass P., Vitek S.M., Robins T.
      Cell Growth Differ. 1:119-127(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
      Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
      , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
      Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney and Skin.
    4. "Characterization of the human Glvr-1 phosphate transporter/retrovirus receptor gene and promoter region."
      Palmer G., Manen D., Bonjour J.-P., Caverzasio J.
      Gene 226:25-33(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-646.
    5. "Feline leukemia virus subgroup B uses the same cell surface receptor as gibbon ape leukemia virus."
      Takeuchi Y., Vile R.G., Simpson G., O'Hara B., Collins M.K., Weiss R.A.
      J. Virol. 66:1219-1222(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "GLVR1, a receptor for gibbon ape leukemia virus, is homologous to a phosphate permease of Neurospora crassa and is expressed at high levels in the brain and thymus."
      Johann S.V., Gibbons J.J., O'Hara B.
      J. Virol. 66:1635-1640(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TOPOLOGY.
    7. "Definition of a domain of GLVR1 which is necessary for infection by gibbon ape leukemia virus and which is highly polymorphic between species."
      Johann S.V., van Zeijl M., Cekleniak J., O'Hara B.
      J. Virol. 67:6733-6736(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF 550-ASP--VAL-558 AND ASP-550, REGION.
    8. "The cellular receptor for gibbon ape leukemia virus is a novel high affinity sodium-dependent phosphate transporter."
      Olah Z., Lehel C., Anderson W.B., Eiden M.V., Wilson C.A.
      J. Biol. Chem. 269:25426-25431(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    9. "A family of retroviruses that utilize related phosphate transporters for cell entry."
      Miller D.G., Miller A.D.
      J. Virol. 68:8270-8276(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Cell-surface receptors for gibbon ape leukemia virus and amphotropic murine retrovirus are inducible sodium-dependent phosphate symporters."
      Kavanaugh M.P., Miller D.G., Zhang W., Law W., Kozak S.L., Kabat D., Miller A.D.
      Proc. Natl. Acad. Sci. U.S.A. 91:7071-7075(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION.
    11. "Phosphate regulation of vascular smooth muscle cell calcification."
      Jono S., McKee M.D., Murry C.E., Shioi A., Nishizawa Y., Mori K., Morii H., Giachelli C.M.
      Circ. Res. 87:E10-E17(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Reassessing the role of region A in Pit1-mediated viral entry."
      Farrell K.B., Russ J.L., Murthy R.K., Eiden M.V.
      J. Virol. 76:7683-7693(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASP-550, REGION.
    13. "Role of interleukin-8 in PiT-1 expression and CXCR1-mediated inorganic phosphate uptake in chondrocytes."
      Cecil D.L., Rose D.M., Terkeltaub R., Liu-Bryan R.
      Arthritis Rheum. 52:144-154(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    14. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    15. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiS20A1_HUMAN
    AccessioniPrimary (citable) accession number: Q8WUM9
    Secondary accession number(s): Q08344, Q6DHX8, Q9UQ82
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3