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Protein

Sodium-dependent phosphate transporter 1

Gene

SLC20A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-phosphate symporter which plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. May play a role in extracellular matrix and cartilage calcification as well as in vascular calcification.4 Publications
(Microbial infection) May function as a retroviral receptor as it confers human cells susceptibility to infection to Gibbon Ape Leukemia Virus (GaLV), Simian sarcoma-associated virus (SSAV) and Feline leukemia virus subgroup B (FeLV-B) as well as 10A1 murine leukemia virus (10A1 MLV).3 Publications

Kineticsi

With an increase in pH, a decrease in phosphate uptake is observed.
  1. KM=24.1 µM for phosphate2 Publications
  1. Vmax=1.9 nmol/min/mg enzyme (in the presence of 0.05-2 mM phosphate)2 Publications

pH dependencei

Optimum pH is 6.5 and 7.5.2 Publications

GO - Molecular functioni

  • high-affinity inorganic phosphate:sodium symporter activity Source: Ensembl
  • inorganic phosphate transmembrane transporter activity Source: GO_Central
  • receptor activity Source: ProtInc
  • signal transducer activity Source: UniProtKB
  • sodium:inorganic phosphate symporter activity Source: GO_Central
  • sodium:phosphate symporter activity Source: ProtInc
  • sodium-dependent phosphate transmembrane transporter activity Source: Reactome

GO - Biological processi

  • ion transport Source: Reactome
  • phosphate-containing compound metabolic process Source: ProtInc
  • positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  • transport Source: ProtInc

Keywordsi

Molecular functionReceptor
Biological processPhosphate transport, Symport, Transport

Enzyme and pathway databases

BRENDAi3.6.3.27. 2681.
ReactomeiR-HSA-427652. Sodium-coupled phosphate cotransporters.
SIGNORiQ8WUM9.

Protein family/group databases

TCDBi2.A.20.2.7. the inorganic phosphate transporter (pit) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent phosphate transporter 1
Alternative name(s):
Gibbon ape leukemia virus receptor 1
Short name:
GLVR-1
Leukemia virus receptor 1 homolog
Phosphate transporter 1
Short name:
PiT-1
Solute carrier family 20 member 1
Gene namesi
Name:SLC20A1
Synonyms:GLVR1, PIT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000144136.10.
HGNCiHGNC:10946. SLC20A1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21
Transmembranei62 – 82HelicalSequence analysisAdd BLAST21
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Transmembranei203 – 223HelicalSequence analysisAdd BLAST21
Transmembranei230 – 250HelicalSequence analysisAdd BLAST21
Transmembranei511 – 531HelicalSequence analysisAdd BLAST21
Transmembranei558 – 578HelicalSequence analysisAdd BLAST21
Transmembranei600 – 620HelicalSequence analysisAdd BLAST21
Transmembranei650 – 670HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi550 – 558DTGDVSSKV → KQEASTKA: Loss of virus infectibility. 1 Publication9
Mutagenesisi550D → K: Drastic reduction of virus infectibility, but conserved virus binding ability. 2 Publications1
Mutagenesisi550Missing : Loss of virus infectibility. 2 Publications1

Organism-specific databases

DisGeNETi6574.
OpenTargetsiENSG00000144136.
PharmGKBiPA35833.

Polymorphism and mutation databases

BioMutaiSLC20A1.
DMDMi74730735.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000807711 – 679Sodium-dependent phosphate transporter 1Add BLAST679

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei265PhosphoserineCombined sources1
Modified residuei269PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8WUM9.
MaxQBiQ8WUM9.
PaxDbiQ8WUM9.
PeptideAtlasiQ8WUM9.
PRIDEiQ8WUM9.

PTM databases

iPTMnetiQ8WUM9.
PhosphoSitePlusiQ8WUM9.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Inductioni

By phosphate deprivation as well as by IL8/interleukin-8 in hypertrophic chondrocytes.2 Publications

Gene expression databases

BgeeiENSG00000144136.
CleanExiHS_SLC20A1.
ExpressionAtlasiQ8WUM9. baseline and differential.
GenevisibleiQ8WUM9. HS.

Organism-specific databases

HPAiCAB019279.
HPA035834.

Interactioni

Protein-protein interaction databases

BioGridi112462. 27 interactors.
IntActiQ8WUM9. 6 interactors.
STRINGi9606.ENSP00000272542.

Structurei

3D structure databases

ProteinModelPortaliQ8WUM9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni550 – 558A9

Domaini

Region A confers human cells susceptibility to infection by Gibbon Ape Leukemia Virus (GaLV) and Feline leukemia virus subgroup B (FeLV-B). Substitution of Human SLC20A1 region A by region A of murine SLC20A1 prevents viral infection.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2493. Eukaryota.
COG0306. LUCA.
GeneTreeiENSGT00390000014879.
HOGENOMiHOG000231892.
HOVERGENiHBG053358.
InParanoidiQ8WUM9.
KOiK14640.
OMAiKKIVTAW.
OrthoDBiEOG091G07J5.
PhylomeDBiQ8WUM9.
TreeFamiTF314426.

Family and domain databases

InterProiView protein in InterPro
IPR001204. Phos_transporter.
PANTHERiPTHR11101. PTHR11101. 1 hit.
PfamiView protein in Pfam
PF01384. PHO4. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8WUM9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLITSTTA ATAASGPLVD YLWMLILGFI IAFVLAFSVG ANDVANSFGT
60 70 80 90 100
AVGSGVVTLK QACILASIFE TVGSVLLGAK VSETIRKGLI DVEMYNSTQG
110 120 130 140 150
LLMAGSVSAM FGSAVWQLVA SFLKLPISGT HCIVGATIGF SLVAKGQEGV
160 170 180 190 200
KWSELIKIVM SWFVSPLLSG IMSGILFFLV RAFILHKADP VPNGLRALPV
210 220 230 240 250
FYACTVGINL FSIMYTGAPL LGFDKLPLWG TILISVGCAV FCALIVWFFV
260 270 280 290 300
CPRMKRKIER EIKCSPSESP LMEKKNSLKE DHEETKLSVG DIENKHPVSE
310 320 330 340 350
VGPATVPLQA VVEERTVSFK LGDLEEAPER ERLPSVDLKE ETSIDSTVNG
360 370 380 390 400
AVQLPNGNLV QFSQAVSNQI NSSGHYQYHT VHKDSGLYKE LLHKLHLAKV
410 420 430 440 450
GDCMGDSGDK PLRRNNSYTS YTMAICGMPL DSFRAKEGEQ KGEEMEKLTW
460 470 480 490 500
PNADSKKRIR MDSYTSYCNA VSDLHSASEI DMSVKAEMGL GDRKGSNGSL
510 520 530 540 550
EEWYDQDKPE VSLLFQFLQI LTACFGSFAH GGNDVSNAIG PLVALYLVYD
560 570 580 590 600
TGDVSSKVAT PIWLLLYGGV GICVGLWVWG RRVIQTMGKD LTPITPSSGF
610 620 630 640 650
SIELASALTV VIASNIGLPI STTHCKVGSV VSVGWLRSKK AVDWRLFRNI
660 670
FMAWFVTVPI SGVISAAIMA IFRYVILRM
Length:679
Mass (Da):73,700
Last modified:March 1, 2002 - v1
Checksum:i5545F6E6DC8F5EA8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti376Y → S in AAA52572 (PubMed:2078500).Curated1
Sequence conflicti443E → R in AAD20286 (PubMed:9889306).Curated1
Sequence conflicti487E → A in AAA52572 (PubMed:2078500).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20859 mRNA. Translation: AAA52572.1.
AC079922 Genomic DNA. Translation: AAY14922.1.
BC019944 mRNA. Translation: AAH19944.1.
BC075818 mRNA. Translation: AAH75818.1.
AH007490 Genomic DNA. Translation: AAD20286.1.
CCDSiCCDS2099.1.
PIRiI52822.
RefSeqiNP_005406.3. NM_005415.4.
UniGeneiHs.187946.
Hs.732750.

Genome annotation databases

EnsembliENST00000272542; ENSP00000272542; ENSG00000144136.
GeneIDi6574.
KEGGihsa:6574.
UCSCiuc002tib.4. human.

Similar proteinsi

Entry informationi

Entry nameiS20A1_HUMAN
AccessioniPrimary (citable) accession number: Q8WUM9
Secondary accession number(s): Q08344, Q6DHX8, Q9UQ82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: September 27, 2017
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families