Q8WUH1 (CHUR_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Churchill

Gene names

Name:	CHURC1
Synonyms:	C14orf52, CHCH
ORF Names:	My015

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator that mediates FGF signaling during neural development. Plays a role in the regulation of cell movement By similarity. Does not bind DNA by itself.
Sequence similarities	Belongs to the Churchill family.
Caution	It is uncertain whether Met-1 or Met-28 is the initiator. Some orthologous sequences cannot be extended. The protein analyzed in Ref.6 is derived from a construct starting at Met-28.
Mass spectrometry	Molecular mass is 13033 Da from positions 28 - 139. Determined by MALDI. The mass difference may be explained by bound zinc ions. Ref.6
Sequence caution	The sequence AAG43128.1 differs from that shown. Reason: Frameshift at positions=92 and translation N-terminally extended. The sequence AAH20550.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence BAG51943.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Coding sequence diversity	Alternative splicing
Ligand	Metal-binding Zinc
Molecular function	Activator Developmental protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8WUH1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8WUH1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     86-86: H → PD

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8WUH1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     86-139: HLCKNCHHVIARHEYTFSIMDEFQEYTMLCLLCGKAEDTISILPDDPRQMTLLF → RVYHAVSVMRQSRRYYQYSP

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 139

139

Protein Churchill

PRO_0000089665

Sites

Metal binding

29

1

Zinc 1

Metal binding

32

1

Zinc 1

Metal binding

57

1

Zinc 1

Metal binding

57

1

Zinc 2

Metal binding

60

1

Zinc 2

Metal binding

86

1

Zinc 3

Metal binding

88

1

Zinc 2

Metal binding

91

1

Zinc 2

Metal binding

93

1

Zinc 1

Metal binding

98

1

Zinc 3

Metal binding

115

1

Zinc 3

Metal binding

118

1

Zinc 3

Natural variations

Alternative sequence

86 – 139

54

HLCKN…MTLLF → RVYHAVSVMRQSRRYYQYSP in isoform 3.

VSP_046509

Alternative sequence

86

1

H → PD in isoform 2.

VSP_021021

Experimental info

Sequence conflict

1 – 3

3

Missing in AAH20550. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

139

Helix Strand Turn

Details...

Beta strand

33 – 37

5

Turn

51 – 53

3

Beta strand

58 – 60

3

Beta strand

66 – 76

11

Beta strand

79 – 88

10

Turn

89 – 91

3

Beta strand

94 – 105

12

Beta strand

108 – 115

8

Turn

116 – 118

3

Beta strand

119 – 126

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 29, 2013. Version 2. Checksum: 16B578B7DBC53CF9	FASTA	139	16,111
	10 20 30 40 50 60 MRQPYLSSRE VSSSRKRWRT FPVDCVAMCG DCVEKEYPNR GNTCLENGSF LLNFTGCAVC 70 80 90 100 110 120 SKRDFMLITN KSLKEEDGEE IVTYDHLCKN CHHVIARHEY TFSIMDEFQE YTMLCLLCGK 130 AEDTISILPD DPRQMTLLF
	Isoform 2 [UniParc]. Checksum: 01C9EA7F745A3954	FASTA	140	16,187
	10 20 30 40 50 60 MRQPYLSSRE VSSSRKRWRT FPVDCVAMCG DCVEKEYPNR GNTCLENGSF LLNFTGCAVC 70 80 90 100 110 120 SKRDFMLITN KSLKEEDGEE IVTYDPDLCK NCHHVIARHE YTFSIMDEFQ EYTMLCLLCG 130 140 KAEDTISILP DDPRQMTLLF
	Isoform 3 [UniParc]. Checksum: F97F156E104D76D6	FASTA	105	12,286
	10 20 30 40 50 60 MRQPYLSSRE VSSSRKRWRT FPVDCVAMCG DCVEKEYPNR GNTCLENGSF LLNFTGCAVC 70 80 90 100 SKRDFMLITN KSLKEEDGEE IVTYDRVYHA VSVMRQSRRY YQYSP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The DNA sequence and analysis of human chromosome 14." Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H. , Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J. Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	Mao Y.M., Xie Y., Lin Q., Li Y., Dai J.L., Ying K. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-139 (ISOFORM 2). Tissue: Fetal brain.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 13-139 (ISOFORM 1). Tissue: Trachea.
[6]	"Embryonic neural inducing factor Churchill is not a DNA-binding zinc finger protein: solution structure reveals a solvent-exposed beta-sheet and zinc binuclear cluster." Lee B.M., Buck-Koehntop B.A., Martinez-Yamout M.A., Dyson H.J., Wright P.E. J. Mol. Biol. 371:1274-1289(2007) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 29-136 IN COMPLEX WITH ZINC IONS, LACK OF DNA-BINDING, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AL135745 Genomic DNA. No translation available.
AL139022 Genomic DNA. No translation available.
CH471061 Genomic DNA. Translation: EAW80884.1.
CH471061 Genomic DNA. Translation: EAW80887.1.
BC020550 mRNA. Translation: AAH20550.1. Different initiation.
AF060510 mRNA. Translation: AAG43128.1. Sequence problems.
AK057626 mRNA. Translation: BAG51943.1. Different initiation.

IPI

IPI00102920.
IPI00797096.

RefSeq

NP_001190992.1. NM_001204063.1.
NP_660148.3. NM_145165.3.

UniGene

Hs.731537.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JOX	NMR	-	A	2-107	[»]

ProteinModelPortal

Q8WUH1.

ModBase

Search...

Polymorphism databases

DMDM

31340022.

Proteomic databases

PaxDb

Q8WUH1.

PeptideAtlas

Q8WUH1.

PRIDE

Q8WUH1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000359118; ENSP00000352026; ENSG00000258289.
ENST00000549115; ENSP00000448050; ENSG00000258289.
ENST00000552002; ENSP00000450144; ENSG00000258289.

GeneID

91612.

KEGG

hsa:91612.

UCSC

uc001xhv.2. human.
uc001xhw.2. human.

Organism-specific databases

CTD

91612.

GeneCards

GC14P065383.
GC14P065384.

HGNC

HGNC:20099. CHURC1.

HPA

CAB009260.
HPA040072.

MIM

608577. gene.

neXtProt

NX_Q8WUH1.

PharmGKB

PA134982480.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5029.

HOGENOM

HOG000246424.

HOVERGEN

HBG039391.

InParanoid

Q8WUH1.

OrthoDB

EOG470TJQ.

PhylomeDB

Q8WUH1.

Gene expression databases

ArrayExpress

Q8WUH1.

Bgee

Q8WUH1.

CleanEx

HS_CHURC1.

Genevestigator

Q8WUH1.

GermOnline

ENSG00000197195. Homo sapiens.

Family and domain databases

InterPro

IPR009508. Transcrpt_activator_Churchill.
[Graphical view]

Pfam

PF06573. Churchill. 1 hit.
[Graphical view]

ProDom

PD355092. Churchill. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

ChiTaRS

CHURC1. human.

EvolutionaryTrace

Q8WUH1.

GenomeRNAi

91612.

NextBio

77335.

SOURCE

Search...

Entry information

Entry name

CHUR_HUMAN

Accession

Primary (citable) accession number: Q8WUH1
Secondary accession number(s): B3KQ81 , G3V1X3, G3V214, Q9H3K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2003
Last sequence update:	May 29, 2013
Last modified:	May 29, 2013
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.