Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RelA-associated inhibitor

Gene

PPP1R13L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.4 Publications

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • identical protein binding Source: IntAct
  • transcription corepressor activity Source: ProtInc
  • transcription factor binding Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Apoptosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-6804759. Regulation of TP53 Activity through Association with Co-factors.

Names & Taxonomyi

Protein namesi
Recommended name:
RelA-associated inhibitor
Alternative name(s):
Inhibitor of ASPP protein
Short name:
Protein iASPP
NFkB-interacting protein 1
PPP1R13B-like protein
Gene namesi
Name:PPP1R13L
Synonyms:IASPP, NKIP1, PPP1R13BL, RAI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:18838. PPP1R13L.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cell junction Source: HPA
  • cytoplasm Source: HPA
  • intercellular bridge Source: HPA
  • nucleoplasm Source: Reactome
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi10848.
OpenTargetsiENSG00000104881.
PharmGKBiPA34195.

Polymorphism and mutation databases

BioMutaiPPP1R13L.
DMDMi92090607.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000669661 – 828RelA-associated inhibitorAdd BLAST828

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei84PhosphoserineCombined sources1
Modified residuei100PhosphoserineCombined sources1
Modified residuei102PhosphoserineCombined sources1
Modified residuei110PhosphoserineCombined sources1
Modified residuei113PhosphoserineCombined sources1
Modified residuei119PhosphoserineCombined sources1
Modified residuei120PhosphoserineCombined sources1
Modified residuei123PhosphothreonineCombined sources1
Modified residuei134PhosphoserineCombined sources1
Modified residuei137Omega-N-methylarginineBy similarity1
Modified residuei142Omega-N-methylarginineBy similarity1
Modified residuei144Omega-N-methylarginineBy similarity1
Modified residuei160Omega-N-methylarginineBy similarity1
Modified residuei167Omega-N-methylarginineCombined sources1
Modified residuei180Omega-N-methylarginineCombined sources1
Modified residuei183PhosphoserineCombined sources1
Modified residuei187PhosphoserineCombined sources1
Modified residuei203PhosphoserineCombined sources1
Modified residuei205Omega-N-methylarginineBy similarity1
Modified residuei280PhosphoserineCombined sources1
Modified residuei308PhosphothreonineCombined sources1
Modified residuei316PhosphoserineCombined sources1
Modified residuei332PhosphoserineCombined sources1
Modified residuei339PhosphoserineCombined sources1
Modified residuei341PhosphothreonineCombined sources1
Modified residuei526PhosphoserineCombined sources1
Modified residuei567PhosphoserineCombined sources1
Modified residuei597PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ8WUF5.
MaxQBiQ8WUF5.
PaxDbiQ8WUF5.
PeptideAtlasiQ8WUF5.
PRIDEiQ8WUF5.

PTM databases

iPTMnetiQ8WUF5.
PhosphoSitePlusiQ8WUF5.

Miscellaneous databases

PMAP-CutDBQ8WUF5.

Expressioni

Tissue specificityi

Highly expressed in heart, placenta and prostate. Weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte.1 Publication

Gene expression databases

BgeeiENSG00000104881.
CleanExiHS_PPP1R13L.
ExpressionAtlasiQ8WUF5. baseline and differential.
GenevisibleiQ8WUF5. HS.

Organism-specific databases

HPAiCAB005016.
HPA041231.
HPA059883.

Interactioni

Subunit structurei

Interacts with RELA NF-kappa-B subunit and with SP1 via its C-terminus part. Interacts with p53/TP53, TP63 and TP73.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-5550163,EBI-5550163
Q9WMX22EBI-5550163,EBI-6863748From a different organism.
EP300Q094722EBI-5550163,EBI-447295
PPP1CAP621365EBI-5550163,EBI-357253
PPP1CBP621403EBI-5550163,EBI-352350
RANP628269EBI-5550163,EBI-286642
TP53P0463711EBI-5550163,EBI-366083

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • identical protein binding Source: IntAct
  • transcription factor binding Source: ProtInc

Protein-protein interaction databases

BioGridi116059. 26 interactors.
DIPiDIP-29586N.
IntActiQ8WUF5. 23 interactors.
MINTiMINT-7034564.
STRINGi9606.ENSP00000354218.

Structurei

Secondary structure

1828
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi627 – 637Combined sources11
Helixi640 – 649Combined sources10
Helixi663 – 669Combined sources7
Helixi673 – 681Combined sources9
Helixi696 – 702Combined sources7
Helixi706 – 713Combined sources8
Turni714 – 716Combined sources3
Helixi731 – 733Combined sources3
Helixi741 – 754Combined sources14
Turni755 – 757Combined sources3
Helixi759 – 761Combined sources3
Beta strandi762 – 767Combined sources6
Beta strandi784 – 791Combined sources8
Beta strandi796 – 803Combined sources8
Beta strandi806 – 811Combined sources6
Helixi812 – 814Combined sources3
Beta strandi815 – 818Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VGEX-ray2.10A607-828[»]
ProteinModelPortaliQ8WUF5.
SMRiQ8WUF5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WUF5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati659 – 691ANK 1Add BLAST33
Repeati692 – 724ANK 2Add BLAST33
Domaini758 – 820SH3PROSITE-ProRule annotationAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi54 – 602Pro-richAdd BLAST549
Compositional biasi407 – 444Gln-richAdd BLAST38

Domaini

The N-terminal region is required for cytoplasmic localization.1 Publication

Sequence similaritiesi

Belongs to the ASPP family.Curated
Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0515. Eukaryota.
ENOG410Y17W. LUCA.
GeneTreeiENSGT00760000119141.
HOVERGENiHBG055210.
InParanoidiQ8WUF5.
OMAiMGLMHNG.
OrthoDBiEOG091G0R1R.
PhylomeDBiQ8WUF5.
TreeFamiTF105545.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR028320. iASPP.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR24164. PTHR24164. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00248. ANK. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8WUF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSEAFQSAR DFLDMNFQSL AMKHMDLKQM ELDTAAAKVD ELTKQLESLW
60 70 80 90 100
SDSPAPPGPQ AGPPSRPPRY SSSSIPEPFG SRGSPRKAAT DGADTPFGRS
110 120 130 140 150
ESAPTLHPYS PLSPKGRPSS PRTPLYLQPD AYGSLDRATS PRPRAFDGAG
160 170 180 190 200
SSLGRAPSPR PGPGPLRQQG PPTPFDFLGR AGSPRGSPLA EGPQAFFPER
210 220 230 240 250
GPSPRPPATA YDAPASAFGS SLLGSGGSAF APPLRAQDDL TLRRRPPKAW
260 270 280 290 300
NESDLDVAYE KKPSQTASYE RLDVFARPAS PSLQLLPWRE SSLDGLGGTG
310 320 330 340 350
KDNLTSATLP RNYKVSPLAS DRRSDAGSYR RSLGSAGPSG TLPRSWQPVS
360 370 380 390 400
RIPMPPSSPQ PRGAPRQRPI PLSMIFKLQN AFWEHGASRA MLPGSPLFTR
410 420 430 440 450
APPPKLQPQP QPQPQPQSQP QPQLPPQPQT QPQTPTPAPQ HPQQTWPPVN
460 470 480 490 500
EGPPKPPTEL EPEPEIEGLL TPVLEAGDVD EGPVARPLSP TRLQPALPPE
510 520 530 540 550
AQSVPELEEV ARVLAEIPRP LKRRGSMEQA PAVALPPTHK KQYQQIISRL
560 570 580 590 600
FHRHGGPGPG GPEPELSPIT EGSEARAGPP APAPPAPIPP PAPSQSSPPE
610 620 630 640 650
QPQSMEMRSV LRKAGSPRKA RRARLNPLVL LLDAALTGEL EVVQQAVKEM
660 670 680 690 700
NDPSQPNEEG ITALHNAICG ANYSIVDFLI TAGANVNSPD SHGWTPLHCA
710 720 730 740 750
ASCNDTVICM ALVQHGAAIF ATTLSDGATA FEKCDPYREG YADCATYLAD
760 770 780 790 800
VEQSMGLMNS GAVYALWDYS AEFGDELSFR EGESVTVLRR DGPEETDWWW
810 820
AALHGQEGYV PRNYFGLFPR VKPQRSKV
Length:828
Mass (Da):89,091
Last modified:April 4, 2006 - v4
Checksum:i7BEEF239B7CA9C70
GO

Sequence cautioni

The sequence AAD27004 differs from that shown. Reason: Frameshift at positions 439, 483, 562 and 591.Curated
The sequence AAD27005 differs from that shown. Probable cloning artifact.Curated
The sequence AAD27005 differs from that shown. Reason: Frameshift at positions 483, 562 and 591.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198P → S in AAH64913 (PubMed:15489334).Curated1
Sequence conflicti223L → I in AAW51146 (Ref. 2) Curated1
Sequence conflicti317 – 318PL → AA in AAD27004 (PubMed:10336463).Curated2
Sequence conflicti321D → T in AAD27004 (PubMed:10336463).Curated1
Sequence conflicti369P → S in AAW51146 (Ref. 2) Curated1
Sequence conflicti372Missing in AAW51146 (Ref. 2) Curated1
Sequence conflicti426P → Q in AAD27004 (PubMed:10336463).Curated1
Sequence conflicti439 – 440PQ → HP in AAD27004 (PubMed:10336463).Curated2
Sequence conflicti747Y → S in AAW51146 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ888472 mRNA. Translation: CAI60219.1.
AY869712 mRNA. Translation: AAW51146.1.
DQ314886 Genomic DNA. Translation: ABC40745.1.
BC001475 mRNA. Translation: AAH01475.1.
BC020589 mRNA. Translation: AAH20589.1.
BC032298 mRNA. Translation: AAH32298.1.
BC064913 mRNA. Translation: AAH64913.1.
AF078036 mRNA. Translation: AAD27004.1. Frameshift.
AF078037 mRNA. Translation: AAD27005.1. Sequence problems.
CCDSiCCDS33050.1.
RefSeqiNP_001135974.1. NM_001142502.1.
NP_006654.2. NM_006663.3.
XP_016881666.1. XM_017026177.1.
XP_016881667.1. XM_017026178.1.
UniGeneiHs.466937.

Genome annotation databases

EnsembliENST00000360957; ENSP00000354218; ENSG00000104881.
ENST00000418234; ENSP00000403902; ENSG00000104881.
GeneIDi10848.
KEGGihsa:10848.
UCSCiuc002pbn.4. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ888472 mRNA. Translation: CAI60219.1.
AY869712 mRNA. Translation: AAW51146.1.
DQ314886 Genomic DNA. Translation: ABC40745.1.
BC001475 mRNA. Translation: AAH01475.1.
BC020589 mRNA. Translation: AAH20589.1.
BC032298 mRNA. Translation: AAH32298.1.
BC064913 mRNA. Translation: AAH64913.1.
AF078036 mRNA. Translation: AAD27004.1. Frameshift.
AF078037 mRNA. Translation: AAD27005.1. Sequence problems.
CCDSiCCDS33050.1.
RefSeqiNP_001135974.1. NM_001142502.1.
NP_006654.2. NM_006663.3.
XP_016881666.1. XM_017026177.1.
XP_016881667.1. XM_017026178.1.
UniGeneiHs.466937.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VGEX-ray2.10A607-828[»]
ProteinModelPortaliQ8WUF5.
SMRiQ8WUF5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116059. 26 interactors.
DIPiDIP-29586N.
IntActiQ8WUF5. 23 interactors.
MINTiMINT-7034564.
STRINGi9606.ENSP00000354218.

PTM databases

iPTMnetiQ8WUF5.
PhosphoSitePlusiQ8WUF5.

Polymorphism and mutation databases

BioMutaiPPP1R13L.
DMDMi92090607.

Proteomic databases

EPDiQ8WUF5.
MaxQBiQ8WUF5.
PaxDbiQ8WUF5.
PeptideAtlasiQ8WUF5.
PRIDEiQ8WUF5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000360957; ENSP00000354218; ENSG00000104881.
ENST00000418234; ENSP00000403902; ENSG00000104881.
GeneIDi10848.
KEGGihsa:10848.
UCSCiuc002pbn.4. human.

Organism-specific databases

CTDi10848.
DisGeNETi10848.
GeneCardsiPPP1R13L.
HGNCiHGNC:18838. PPP1R13L.
HPAiCAB005016.
HPA041231.
HPA059883.
MIMi607463. gene.
neXtProtiNX_Q8WUF5.
OpenTargetsiENSG00000104881.
PharmGKBiPA34195.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0515. Eukaryota.
ENOG410Y17W. LUCA.
GeneTreeiENSGT00760000119141.
HOVERGENiHBG055210.
InParanoidiQ8WUF5.
OMAiMGLMHNG.
OrthoDBiEOG091G0R1R.
PhylomeDBiQ8WUF5.
TreeFamiTF105545.

Enzyme and pathway databases

ReactomeiR-HSA-6804759. Regulation of TP53 Activity through Association with Co-factors.

Miscellaneous databases

ChiTaRSiPPP1R13L. human.
EvolutionaryTraceiQ8WUF5.
GeneWikiiPPP1R13L.
GenomeRNAii10848.
PMAP-CutDBQ8WUF5.
PROiQ8WUF5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000104881.
CleanExiHS_PPP1R13L.
ExpressionAtlasiQ8WUF5. baseline and differential.
GenevisibleiQ8WUF5. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR028320. iASPP.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR24164. PTHR24164. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00248. ANK. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 2 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIASPP_HUMAN
AccessioniPrimary (citable) accession number: Q8WUF5
Secondary accession number(s): Q2PNZ9
, Q5DU71, Q5I1X4, Q6P1R7, Q6PKF8, Q9Y290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: April 4, 2006
Last modified: November 30, 2016
This is version 142 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

An alternative product iASPP(RAI) has been described (PubMed:15489900, PubMed:10336463). However, it is not detected in vivo and is most probably a cloning artifact (PubMed:15489900).2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.