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Protein

Cholinephosphotransferase 1

Gene

CHPT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes phosphatidylcholine biosynthesis from CDP-choline. It thereby plays a central role in the formation and maintenance of vesicular membranes.

Catalytic activityi

CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase A (PCYT1A), Choline-phosphate cytidylyltransferase B (PCYT1B)
  2. Cholinephosphotransferase 1 (CHPT1), Choline/ethanolaminephosphotransferase 1 (CEPT1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • diacylglycerol binding Source: UniProtKB
  • diacylglycerol cholinephosphotransferase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • CDP-choline pathway Source: UniProtKB
  • lipid metabolic process Source: ProtInc
  • phosphatidylcholine biosynthetic process Source: UniProtKB
  • platelet activating factor biosynthetic process Source: UniProtKB
  • regulation of cell growth Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.7.8.2. 2681.
ReactomeiR-HSA-1483191. Synthesis of PC.
UniPathwayiUPA00753; UER00740.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinephosphotransferase 1 (EC:2.7.8.2)
Short name:
hCPT1
Alternative name(s):
AAPT1-like protein
Diacylglycerol cholinephosphotransferase 1
Gene namesi
Name:CHPT1
Synonyms:CPT1
ORF Names:MSTP022
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:17852. CHPT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei66 – 8621HelicalSequence analysisAdd
BLAST
Transmembranei93 – 11321HelicalSequence analysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence analysisAdd
BLAST
Transmembranei193 – 21321HelicalSequence analysisAdd
BLAST
Transmembranei226 – 24823HelicalSequence analysisAdd
BLAST
Transmembranei261 – 28121HelicalSequence analysisAdd
BLAST
Transmembranei295 – 31521HelicalSequence analysisAdd
BLAST
Transmembranei349 – 36921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26477.

Polymorphism and mutation databases

BioMutaiCHPT1.
DMDMi74730698.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 406405Cholinephosphotransferase 1PRO_0000289252Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8WUD6.
MaxQBiQ8WUD6.
PaxDbiQ8WUD6.
PRIDEiQ8WUD6.

PTM databases

iPTMnetiQ8WUD6.
PhosphoSiteiQ8WUD6.

Expressioni

Tissue specificityi

Highly expressed in testis, colon, small intestine, heart, prostate and spleen. Also detected in kidney, skeletal muscle, pancreas, leukocytes, ovary and thymus. Weakly expressed in the brain, placenta and lung. Overexpressed in cancerous breast epithelial cell lines.2 Publications

Gene expression databases

BgeeiQ8WUD6.
CleanExiHS_CHPT1.
ExpressionAtlasiQ8WUD6. baseline and differential.
GenevisibleiQ8WUD6. HS.

Organism-specific databases

HPAiHPA067643.

Interactioni

Protein-protein interaction databases

BioGridi121309. 30 interactions.
IntActiQ8WUD6. 1 interaction.
STRINGi9606.ENSP00000229266.

Structurei

3D structure databases

ProteinModelPortaliQ8WUD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2877. Eukaryota.
COG5050. LUCA.
GeneTreeiENSGT00530000063048.
HOVERGENiHBG107647.
InParanoidiQ8WUD6.
KOiK00994.
OMAiMAPNSIT.
OrthoDBiEOG7SR4MH.
PhylomeDBiQ8WUD6.
TreeFamiTF313270.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
[Graphical view]
PANTHERiPTHR10414. PTHR10414. 1 hit.
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF015665. CHOPT. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WUD6-1) [UniParc]FASTAAdd to basket

Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGAGAGSA PRWLRALSEP LSAAQLRRLE EHRYSAAGVS LLEPPLQLYW
60 70 80 90 100
TWLLQWIPLW MAPNSITLLG LAVNVVTTLV LISYCPTATE EAPYWTYLLC
110 120 130 140 150
ALGLFIYQSL DAIDGKQARR TNSCSPLGEL FDHGCDSLST VFMAVGASIA
160 170 180 190 200
ARLGTYPDWF FFCSFIGMFV FYCAHWQTYV SGMLRFGKVD VTEIQIALVI
210 220 230 240 250
VFVLSAFGGA TMWDYTIPIL EIKLKILPVL GFLGGVIFSC SNYFHVILHG
260 270 280 290 300
GVGKNGSTIA GTSVLSPGLH IGLIIILAIM IYKKSATDVF EKHPCLYILM
310 320 330 340 350
FGCVFAKVSQ KLVVAHMTKS ELYLQDTVFL GPGLLFLDQY FNNFIDEYVV
360 370 380 390 400
LWMAMVISSF DMVIYFSALC LQISRHLHLN IFKTACHQAP EQVQVLSSKS

HQNNMD
Length:406
Mass (Da):45,097
Last modified:March 1, 2002 - v1
Checksum:iAE5357C02ED40E4C
GO
Isoform 2 (identifier: Q8WUD6-2) [UniParc]FASTAAdd to basket

Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     217-218: IP → FS
     219-406: Missing.

Show »
Length:218
Mass (Da):24,154
Checksum:i576CFC9B1E4F64F9
GO

Sequence cautioni

The sequence AAD44019.1 differs from that shown. Reason: Frameshift at position 310. Curated
The sequence AAL39005.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861P → L in AAD44019 (Ref. 5) Curated
Sequence conflicti109 – 1091S → P in AAD44019 (Ref. 5) Curated
Sequence conflicti269 – 2691L → P in AAD44019 (Ref. 5) Curated
Sequence conflicti346 – 3461D → G in AAD44019 (Ref. 5) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti162 – 1621F → S.1 Publication
Corresponds to variant rs3205421 [ dbSNP | Ensembl ].
VAR_032612
Natural varianti323 – 3231Y → S in MCF-12A cell line. 1 Publication
VAR_032613

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei217 – 2182IP → FS in isoform 2. 1 PublicationVSP_025989
Alternative sequencei219 – 406188Missing in isoform 2. 1 PublicationVSP_025990Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195623 mRNA. Translation: AAF87947.1.
AF195624 mRNA. Translation: AAF87948.1.
AK075211 mRNA. Translation: BAG52084.1.
CH471054 Genomic DNA. Translation: EAW97675.1.
BC020819 mRNA. Translation: AAH20819.1.
BC050429 mRNA. Translation: AAH50429.1.
AF047431 mRNA. Translation: AAD44019.1. Frameshift.
AF111803 mRNA. Translation: AAL39005.1. Different initiation.
AY280609 mRNA. Translation: AAP34412.1.
AY280610 mRNA. Translation: AAP34413.1.
AY294627 mRNA. Translation: AAP37157.1.
AY166717 mRNA. Translation: AAN86122.1.
AY166718 mRNA. Translation: AAN86123.1.
CCDSiCCDS9086.1. [Q8WUD6-1]
RefSeqiNP_064629.2. NM_020244.2. [Q8WUD6-1]
UniGeneiHs.293077.

Genome annotation databases

EnsembliENST00000229266; ENSP00000229266; ENSG00000111666. [Q8WUD6-1]
ENST00000552351; ENSP00000447887; ENSG00000111666. [Q8WUD6-2]
GeneIDi56994.
KEGGihsa:56994.
UCSCiuc001tin.4. human. [Q8WUD6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195623 mRNA. Translation: AAF87947.1.
AF195624 mRNA. Translation: AAF87948.1.
AK075211 mRNA. Translation: BAG52084.1.
CH471054 Genomic DNA. Translation: EAW97675.1.
BC020819 mRNA. Translation: AAH20819.1.
BC050429 mRNA. Translation: AAH50429.1.
AF047431 mRNA. Translation: AAD44019.1. Frameshift.
AF111803 mRNA. Translation: AAL39005.1. Different initiation.
AY280609 mRNA. Translation: AAP34412.1.
AY280610 mRNA. Translation: AAP34413.1.
AY294627 mRNA. Translation: AAP37157.1.
AY166717 mRNA. Translation: AAN86122.1.
AY166718 mRNA. Translation: AAN86123.1.
CCDSiCCDS9086.1. [Q8WUD6-1]
RefSeqiNP_064629.2. NM_020244.2. [Q8WUD6-1]
UniGeneiHs.293077.

3D structure databases

ProteinModelPortaliQ8WUD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121309. 30 interactions.
IntActiQ8WUD6. 1 interaction.
STRINGi9606.ENSP00000229266.

PTM databases

iPTMnetiQ8WUD6.
PhosphoSiteiQ8WUD6.

Polymorphism and mutation databases

BioMutaiCHPT1.
DMDMi74730698.

Proteomic databases

EPDiQ8WUD6.
MaxQBiQ8WUD6.
PaxDbiQ8WUD6.
PRIDEiQ8WUD6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000229266; ENSP00000229266; ENSG00000111666. [Q8WUD6-1]
ENST00000552351; ENSP00000447887; ENSG00000111666. [Q8WUD6-2]
GeneIDi56994.
KEGGihsa:56994.
UCSCiuc001tin.4. human. [Q8WUD6-1]

Organism-specific databases

CTDi56994.
GeneCardsiCHPT1.
H-InvDBHIX0010924.
HGNCiHGNC:17852. CHPT1.
HPAiHPA067643.
MIMi616747. gene.
neXtProtiNX_Q8WUD6.
PharmGKBiPA26477.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2877. Eukaryota.
COG5050. LUCA.
GeneTreeiENSGT00530000063048.
HOVERGENiHBG107647.
InParanoidiQ8WUD6.
KOiK00994.
OMAiMAPNSIT.
OrthoDBiEOG7SR4MH.
PhylomeDBiQ8WUD6.
TreeFamiTF313270.

Enzyme and pathway databases

UniPathwayiUPA00753; UER00740.
BRENDAi2.7.8.2. 2681.
ReactomeiR-HSA-1483191. Synthesis of PC.

Miscellaneous databases

ChiTaRSiCHPT1. human.
GenomeRNAii56994.
PROiQ8WUD6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8WUD6.
CleanExiHS_CHPT1.
ExpressionAtlasiQ8WUD6. baseline and differential.
GenevisibleiQ8WUD6. HS.

Family and domain databases

InterProiIPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
[Graphical view]
PANTHERiPTHR10414. PTHR10414. 1 hit.
PfamiPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFiPIRSF015665. CHOPT. 1 hit.
PROSITEiPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, genomic organization, and characterization of a human cholinephosphotransferase."
    Henneberry A.L., Wistow G., McMaster C.R.
    J. Biol. Chem. 275:29808-29815(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ENZYME ACTIVITY, COFACTOR, TISSUE SPECIFICITY, VARIANT SER-162.
    Tissue: Brain.
  2. "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
    Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.
    , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
    DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis and Uterus.
  5. "Human AAPT1-like gene."
    Zhang Q., Mao M., Fu G., Huang Q., Zhou J., Yu Y., Xu S., Shen Y., Huang Q., Wang Y., Chen Z.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 70-406.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 115-406.
    Tissue: Heart.
  7. "Inhibition of cholinephosphotransferase activity in lung injury induced by 2-chloroethyl ethyl sulfide, a mustard analog."
    Sinha Roy S., Mukherjee S., Kabir S., Rajaratnam V., Smith M., Das S.K.
    J. Biochem. Mol. Toxicol. 19:289-297(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 174-406.
  8. "Differential expression of cholinephosphotransferase in normal and cancerous human mammary epithelial cells."
    Ghosh A., Akech J., Mukherjee S., Das S.K.
    Biochem. Biophys. Res. Commun. 297:1043-1048(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 235-371, TISSUE SPECIFICITY, VARIANT SER-323.
  9. "The major sites of cellular phospholipid synthesis and molecular determinants of fatty acid and lipid head group specificity."
    Henneberry A.L., Wright M.M., McMaster C.R.
    Mol. Biol. Cell 13:3148-3161(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCHPT1_HUMAN
AccessioniPrimary (citable) accession number: Q8WUD6
Secondary accession number(s): B3KQM2
, Q7Z7H0, Q7Z7H1, Q7Z7H2, Q8IWQ4, Q8IWQ5, Q8WYI4, Q9NRQ6, Q9NRQ7, Q9Y6M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2002
Last modified: June 8, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.