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Q8WUD6 (CHPT1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholinephosphotransferase 1
Short name=hCPT1
EC=2.7.8.2
Alternative name(s):
AAPT1-like protein
Diacylglycerol cholinephosphotransferase 1
Gene names
Name:CHPT1
Synonyms:CPT1
ORF Names:MSTP022
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes phosphatidylcholine biosynthesis from CDP-choline. It thereby plays a central role in the formation and maintenance of vesicular membranes.

Catalytic activity

CDP-choline + 1,2-diacylglycerol = CMP + a phosphatidylcholine. Ref.1

Cofactor

Magnesium or manganese. Ref.1

Pathway

Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 2/2.

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein Ref.9.

Tissue specificity

Highly expressed in testis, colon, small intestine, heart, prostate and spleen. Also detected in kidney, skeletal muscle, pancreas, leukocytes, ovary and thymus. Weakly expressed in the brain, placenta and lung. Overexpressed in cancerous breast epithelial cell lines. Ref.1 Ref.8

Sequence similarities

Belongs to the CDP-alcohol phosphatidyltransferase class-I family.

Sequence caution

The sequence AAD44019.1 differs from that shown. Reason: Frameshift at position 310.

The sequence AAL39005.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WUD6-1)

Also known as: Alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WUD6-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     217-218: IP → FS
     219-406: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 406405Cholinephosphotransferase 1
PRO_0000289252

Regions

Transmembrane66 – 8621Helical; Potential
Transmembrane93 – 11321Helical; Potential
Transmembrane153 – 17321Helical; Potential
Transmembrane193 – 21321Helical; Potential
Transmembrane226 – 24823Helical; Potential
Transmembrane261 – 28121Helical; Potential
Transmembrane295 – 31521Helical; Potential
Transmembrane349 – 36921Helical; Potential

Amino acid modifications

Modified residue21N-acetylalanine Ref.10

Natural variations

Alternative sequence217 – 2182IP → FS in isoform 2.
VSP_025989
Alternative sequence219 – 406188Missing in isoform 2.
VSP_025990
Natural variant1621F → S. Ref.1
Corresponds to variant rs3205421 [ dbSNP | Ensembl ].
VAR_032612
Natural variant3231Y → S in MCF-12A cell line. Ref.8
VAR_032613

Experimental info

Sequence conflict861P → L in AAD44019. Ref.5
Sequence conflict1091S → P in AAD44019. Ref.5
Sequence conflict2691L → P in AAD44019. Ref.5
Sequence conflict3461D → G in AAD44019. Ref.5

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Alpha) [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: AE5357C02ED40E4C

FASTA40645,097
        10         20         30         40         50         60 
MAAGAGAGSA PRWLRALSEP LSAAQLRRLE EHRYSAAGVS LLEPPLQLYW TWLLQWIPLW 

        70         80         90        100        110        120 
MAPNSITLLG LAVNVVTTLV LISYCPTATE EAPYWTYLLC ALGLFIYQSL DAIDGKQARR 

       130        140        150        160        170        180 
TNSCSPLGEL FDHGCDSLST VFMAVGASIA ARLGTYPDWF FFCSFIGMFV FYCAHWQTYV 

       190        200        210        220        230        240 
SGMLRFGKVD VTEIQIALVI VFVLSAFGGA TMWDYTIPIL EIKLKILPVL GFLGGVIFSC 

       250        260        270        280        290        300 
SNYFHVILHG GVGKNGSTIA GTSVLSPGLH IGLIIILAIM IYKKSATDVF EKHPCLYILM 

       310        320        330        340        350        360 
FGCVFAKVSQ KLVVAHMTKS ELYLQDTVFL GPGLLFLDQY FNNFIDEYVV LWMAMVISSF 

       370        380        390        400 
DMVIYFSALC LQISRHLHLN IFKTACHQAP EQVQVLSSKS HQNNMD

« Hide

Isoform 2 (Beta) [UniParc].

Checksum: 576CFC9B1E4F64F9
Show »

FASTA21824,154

References

« Hide 'large scale' references
[1]"Cloning, genomic organization, and characterization of a human cholinephosphotransferase."
Henneberry A.L., Wistow G., McMaster C.R.
J. Biol. Chem. 275:29808-29815(2000) [PubMed: 10893425] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ENZYME ACTIVITY, COFACTOR, TISSUE SPECIFICITY, VARIANT SER-162.
Tissue: Brain.
[2]"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. expand/collapse author list , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T.
DNA Res. 12:117-126(2005) [PubMed: 16303743] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Placenta.
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Testis and Uterus.
[5]"Human AAPT1-like gene."
Zhang Q., Mao M., Fu G., Huang Q., Zhou J., Yu Y., Xu S., Shen Y., Huang Q., Wang Y., Chen Z.
Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 70-406.
[6]Xu Y.Y., Sun L.Z., Wu Q.Y., Liu Y.Q., Liu B., Zhao B., Wang X.Y., Song L., Ye J., Sheng H., Gao Y., Zhang C.L., Zhang J., Wei Y.J., Sun Y.H., Jiang Y.X., Zhao X.W., Liu S. expand/collapse author list , Liu L.S., Ding J.F., Gao R.L., Qiang B.Q., Yuan J.G., Liew C.C., Zhao M.S., Hui R.T.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 115-406.
Tissue: Heart.
[7]"Inhibition of cholinephosphotransferase activity in lung injury induced by 2-chloroethyl ethyl sulfide, a mustard analog."
Sinha Roy S., Mukherjee S., Kabir S., Rajaratnam V., Smith M., Das S.K.
J. Biochem. Mol. Toxicol. 19:289-297(2005) [PubMed: 16292752] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 174-406.
[8]"Differential expression of cholinephosphotransferase in normal and cancerous human mammary epithelial cells."
Ghosh A., Akech J., Mukherjee S., Das S.K.
Biochem. Biophys. Res. Commun. 297:1043-1048(2002) [PubMed: 12359261] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 235-371, TISSUE SPECIFICITY, VARIANT SER-323.
[9]"The major sites of cellular phospholipid synthesis and molecular determinants of fatty acid and lipid head group specificity."
Henneberry A.L., Wright M.M., McMaster C.R.
Mol. Biol. Cell 13:3148-3161(2002) [PubMed: 12221122] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY.
Tissue: Embryonic kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF195623 mRNA. Translation: AAF87947.1.
AF195624 mRNA. Translation: AAF87948.1.
AK075211 mRNA. Translation: BAG52084.1.
CH471054 Genomic DNA. Translation: EAW97675.1.
BC020819 mRNA. Translation: AAH20819.1.
BC050429 mRNA. Translation: AAH50429.1.
AF047431 mRNA. Translation: AAD44019.1. Frameshift.
AF111803 mRNA. Translation: AAL39005.1. Different initiation.
AY280609 mRNA. Translation: AAP34412.1.
AY280610 mRNA. Translation: AAP34413.1.
AY294627 mRNA. Translation: AAP37157.1.
AY166717 mRNA. Translation: AAN86122.1.
AY166718 mRNA. Translation: AAN86123.1.
IPIIPI00329548.
IPI00792004.
RefSeqNP_064629.2. NM_020244.2.
UniGeneHs.293077.

3D structure databases

ProteinModelPortalQ8WUD6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8WUD6.

PTM databases

PhosphoSiteQ8WUD6.

Polymorphism databases

DMDM74730698.

Proteomic databases

PRIDEQ8WUD6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000229266; ENSP00000229266; ENSG00000111666.
GeneID56994.
KEGGhsa:56994.
UCSCuc001tin.1. human.
uc001tip.1. human.

Organism-specific databases

CTD56994.
GeneCardsGC12P102074.
H-InvDBHIX0010924.
HGNCHGNC:17852. CHPT1.
neXtProtNX_Q8WUD6.
PharmGKBPA26477.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG16251.
GeneTreeENSGT00530000063048.
HOVERGENHBG107647.
InParanoidQ8WUD6.
OMAPILEIKW.
OrthoDBEOG4V6ZGJ.
PhylomeDBQ8WUD6.

Enzyme and pathway databases

BRENDA2.7.8.2. 2681.

Gene expression databases

ArrayExpressQ8WUD6.
BgeeQ8WUD6.
CleanExHS_CHPT1.
GenevestigatorQ8WUD6.

Family and domain databases

InterProIPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
[Graphical view]
KOK00994.
PfamPF01066. CDP-OH_P_transf. 1 hit.
[Graphical view]
PIRSFPIRSF015665. CHOPT. 1 hit.
PROSITEPS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio62697.

Entry information

Entry nameCHPT1_HUMAN
AccessionPrimary (citable) accession number: Q8WUD6
Secondary accession number(s): B3KQM2 expand/collapse secondary AC list , Q7Z7H0, Q7Z7H1, Q7Z7H2, Q8IWQ4, Q8IWQ5, Q8WYI4, Q9NRQ6, Q9NRQ7, Q9Y6M6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2002
Last modified: January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents