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Protein

Cholinephosphotransferase 1

Gene

CHPT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes phosphatidylcholine biosynthesis from CDP-choline. It thereby plays a central role in the formation and maintenance of vesicular membranes.

Catalytic activityi

CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase A (PCYT1A), Choline-phosphate cytidylyltransferase B (PCYT1B)
  2. Cholinephosphotransferase 1 (CHPT1), Choline/ethanolaminephosphotransferase 1 (CEPT1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • diacylglycerol binding Source: UniProtKB
  • diacylglycerol cholinephosphotransferase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • CDP-choline pathway Source: UniProtKB
  • lipid metabolic process Source: ProtInc
  • phosphatidylcholine biosynthetic process Source: UniProtKB
  • platelet activating factor biosynthetic process Source: UniProtKB
  • regulation of cell growth Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.7.8.2. 2681.
ReactomeiR-HSA-1483191. Synthesis of PC.
UniPathwayiUPA00753; UER00740.

Chemistry databases

SwissLipidsiSLP:000001750. [Q8WUD6-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinephosphotransferase 1 (EC:2.7.8.2)
Short name:
hCPT1
Alternative name(s):
AAPT1-like protein
Diacylglycerol cholinephosphotransferase 1
Gene namesi
Name:CHPT1
Synonyms:CPT1
ORF Names:MSTP022
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111666.10.
HGNCiHGNC:17852. CHPT1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Transmembranei226 – 248HelicalSequence analysisAdd BLAST23
Transmembranei261 – 281HelicalSequence analysisAdd BLAST21
Transmembranei295 – 315HelicalSequence analysisAdd BLAST21
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi56994.
OpenTargetsiENSG00000111666.
PharmGKBiPA26477.

Polymorphism and mutation databases

BioMutaiCHPT1.
DMDMi74730698.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002892522 – 406Cholinephosphotransferase 1Add BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8WUD6.
MaxQBiQ8WUD6.
PaxDbiQ8WUD6.
PeptideAtlasiQ8WUD6.
PRIDEiQ8WUD6.

PTM databases

iPTMnetiQ8WUD6.
PhosphoSitePlusiQ8WUD6.

Expressioni

Tissue specificityi

Highly expressed in testis, colon, small intestine, heart, prostate and spleen. Also detected in kidney, skeletal muscle, pancreas, leukocytes, ovary and thymus. Weakly expressed in the brain, placenta and lung. Overexpressed in cancerous breast epithelial cell lines.2 Publications

Gene expression databases

BgeeiENSG00000111666.
CleanExiHS_CHPT1.
ExpressionAtlasiQ8WUD6. baseline and differential.
GenevisibleiQ8WUD6. HS.

Organism-specific databases

HPAiHPA067643.

Interactioni

Protein-protein interaction databases

BioGridi121309. 32 interactors.
IntActiQ8WUD6. 2 interactors.
STRINGi9606.ENSP00000229266.

Structurei

3D structure databases

ProteinModelPortaliQ8WUD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2877. Eukaryota.
COG5050. LUCA.
GeneTreeiENSGT00530000063048.
HOVERGENiHBG107647.
InParanoidiQ8WUD6.
KOiK00994.
OMAiMAPNSIT.
OrthoDBiEOG091G07XY.
PhylomeDBiQ8WUD6.
TreeFamiTF313270.

Family and domain databases

InterProiView protein in InterPro
IPR000462. CDP-OH_P_trans.
IPR014472. CHOPT.
PANTHERiPTHR10414. PTHR10414. 1 hit.
PfamiView protein in Pfam
PF01066. CDP-OH_P_transf. 1 hit.
PIRSFiPIRSF015665. CHOPT. 1 hit.
PROSITEiView protein in PROSITE
PS00379. CDP_ALCOHOL_P_TRANSF. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WUD6-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGAGAGSA PRWLRALSEP LSAAQLRRLE EHRYSAAGVS LLEPPLQLYW
60 70 80 90 100
TWLLQWIPLW MAPNSITLLG LAVNVVTTLV LISYCPTATE EAPYWTYLLC
110 120 130 140 150
ALGLFIYQSL DAIDGKQARR TNSCSPLGEL FDHGCDSLST VFMAVGASIA
160 170 180 190 200
ARLGTYPDWF FFCSFIGMFV FYCAHWQTYV SGMLRFGKVD VTEIQIALVI
210 220 230 240 250
VFVLSAFGGA TMWDYTIPIL EIKLKILPVL GFLGGVIFSC SNYFHVILHG
260 270 280 290 300
GVGKNGSTIA GTSVLSPGLH IGLIIILAIM IYKKSATDVF EKHPCLYILM
310 320 330 340 350
FGCVFAKVSQ KLVVAHMTKS ELYLQDTVFL GPGLLFLDQY FNNFIDEYVV
360 370 380 390 400
LWMAMVISSF DMVIYFSALC LQISRHLHLN IFKTACHQAP EQVQVLSSKS

HQNNMD
Length:406
Mass (Da):45,097
Last modified:March 1, 2002 - v1
Checksum:iAE5357C02ED40E4C
GO
Isoform 2 (identifier: Q8WUD6-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     217-218: IP → FS
     219-406: Missing.

Show »
Length:218
Mass (Da):24,154
Checksum:i576CFC9B1E4F64F9
GO

Sequence cautioni

The sequence AAD44019 differs from that shown. Reason: Frameshift at position 310.Curated
The sequence AAL39005 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86P → L in AAD44019 (Ref. 5) Curated1
Sequence conflicti109S → P in AAD44019 (Ref. 5) Curated1
Sequence conflicti269L → P in AAD44019 (Ref. 5) Curated1
Sequence conflicti346D → G in AAD44019 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_032612162F → S1 PublicationCorresponds to variant dbSNP:rs3205421Ensembl.1
Natural variantiVAR_032613323Y → S in MCF-12A cell line. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025989217 – 218IP → FS in isoform 2. 1 Publication2
Alternative sequenceiVSP_025990219 – 406Missing in isoform 2. 1 PublicationAdd BLAST188

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195623 mRNA. Translation: AAF87947.1.
AF195624 mRNA. Translation: AAF87948.1.
AK075211 mRNA. Translation: BAG52084.1.
CH471054 Genomic DNA. Translation: EAW97675.1.
BC020819 mRNA. Translation: AAH20819.1.
BC050429 mRNA. Translation: AAH50429.1.
AF047431 mRNA. Translation: AAD44019.1. Frameshift.
AF111803 mRNA. Translation: AAL39005.1. Different initiation.
AY280609 mRNA. Translation: AAP34412.1.
AY280610 mRNA. Translation: AAP34413.1.
AY294627 mRNA. Translation: AAP37157.1.
AY166717 mRNA. Translation: AAN86122.1.
AY166718 mRNA. Translation: AAN86123.1.
CCDSiCCDS9086.1. [Q8WUD6-1]
RefSeqiNP_064629.2. NM_020244.2. [Q8WUD6-1]
UniGeneiHs.293077.

Genome annotation databases

EnsembliENST00000229266; ENSP00000229266; ENSG00000111666. [Q8WUD6-1]
ENST00000552351; ENSP00000447887; ENSG00000111666. [Q8WUD6-2]
GeneIDi56994.
KEGGihsa:56994.
UCSCiuc001tin.4. human. [Q8WUD6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCHPT1_HUMAN
AccessioniPrimary (citable) accession number: Q8WUD6
Secondary accession number(s): B3KQM2
, Q7Z7H0, Q7Z7H1, Q7Z7H2, Q8IWQ4, Q8IWQ5, Q8WYI4, Q9NRQ6, Q9NRQ7, Q9Y6M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2002
Last modified: October 25, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families