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Protein

Cholinephosphotransferase 1

Gene

CHPT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes phosphatidylcholine biosynthesis from CDP-choline. It thereby plays a central role in the formation and maintenance of vesicular membranes.

Catalytic activityi

CDP-choline + 1,2-diacyl-sn-glycerol = CMP + a phosphatidylcholine.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication

Pathwayi: phosphatidylcholine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylcholine from phosphocholine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Choline-phosphate cytidylyltransferase A (PCYT1A), Choline-phosphate cytidylyltransferase B (PCYT1B)
  2. Cholinephosphotransferase 1 (CHPT1), Choline/ethanolaminephosphotransferase 1 (CEPT1)
This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylcholine from phosphocholine, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • diacylglycerol binding Source: UniProtKB
  • diacylglycerol cholinephosphotransferase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • CDP-choline pathway Source: UniProtKB
  • lipid metabolic process Source: ProtInc
  • phosphatidylcholine biosynthetic process Source: UniProtKB
  • platelet activating factor biosynthetic process Source: UniProtKB
  • regulation of cell growth Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi2.7.8.2 2681
ReactomeiR-HSA-1483191 Synthesis of PC
UniPathwayiUPA00753; UER00740

Chemistry databases

SwissLipidsiSLP:000001750 [Q8WUD6-1]

Names & Taxonomyi

Protein namesi
Recommended name:
Cholinephosphotransferase 1 (EC:2.7.8.2)
Short name:
hCPT1
Alternative name(s):
AAPT1-like protein
Diacylglycerol cholinephosphotransferase 1
Gene namesi
Name:CHPT1
Synonyms:CPT1
ORF Names:MSTP022
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000111666.10
HGNCiHGNC:17852 CHPT1
MIMi616747 gene
neXtProtiNX_Q8WUD6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei66 – 86HelicalSequence analysisAdd BLAST21
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Transmembranei226 – 248HelicalSequence analysisAdd BLAST23
Transmembranei261 – 281HelicalSequence analysisAdd BLAST21
Transmembranei295 – 315HelicalSequence analysisAdd BLAST21
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi56994
OpenTargetsiENSG00000111666
PharmGKBiPA26477

Polymorphism and mutation databases

BioMutaiCHPT1
DMDMi74730698

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002892522 – 406Cholinephosphotransferase 1Add BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8WUD6
MaxQBiQ8WUD6
PaxDbiQ8WUD6
PeptideAtlasiQ8WUD6
PRIDEiQ8WUD6

PTM databases

iPTMnetiQ8WUD6
PhosphoSitePlusiQ8WUD6

Expressioni

Tissue specificityi

Highly expressed in testis, colon, small intestine, heart, prostate and spleen. Also detected in kidney, skeletal muscle, pancreas, leukocytes, ovary and thymus. Weakly expressed in the brain, placenta and lung. Overexpressed in cancerous breast epithelial cell lines.2 Publications

Gene expression databases

BgeeiENSG00000111666
CleanExiHS_CHPT1
ExpressionAtlasiQ8WUD6 baseline and differential
GenevisibleiQ8WUD6 HS

Organism-specific databases

HPAiHPA067643

Interactioni

Protein-protein interaction databases

BioGridi121309, 33 interactors
IntActiQ8WUD6, 3 interactors
MINTiQ8WUD6
STRINGi9606.ENSP00000229266

Structurei

3D structure databases

ProteinModelPortaliQ8WUD6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2877 Eukaryota
COG5050 LUCA
GeneTreeiENSGT00530000063048
HOVERGENiHBG107647
InParanoidiQ8WUD6
KOiK00994
OMAiMAPNSIT
OrthoDBiEOG091G07XY
PhylomeDBiQ8WUD6
TreeFamiTF313270

Family and domain databases

InterProiView protein in InterPro
IPR000462 CDP-OH_P_trans
IPR014472 CHOPT
PANTHERiPTHR10414 PTHR10414, 1 hit
PfamiView protein in Pfam
PF01066 CDP-OH_P_transf, 1 hit
PIRSFiPIRSF015665 CHOPT, 1 hit
PROSITEiView protein in PROSITE
PS00379 CDP_ALCOHOL_P_TRANSF, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WUD6-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGAGAGSA PRWLRALSEP LSAAQLRRLE EHRYSAAGVS LLEPPLQLYW
60 70 80 90 100
TWLLQWIPLW MAPNSITLLG LAVNVVTTLV LISYCPTATE EAPYWTYLLC
110 120 130 140 150
ALGLFIYQSL DAIDGKQARR TNSCSPLGEL FDHGCDSLST VFMAVGASIA
160 170 180 190 200
ARLGTYPDWF FFCSFIGMFV FYCAHWQTYV SGMLRFGKVD VTEIQIALVI
210 220 230 240 250
VFVLSAFGGA TMWDYTIPIL EIKLKILPVL GFLGGVIFSC SNYFHVILHG
260 270 280 290 300
GVGKNGSTIA GTSVLSPGLH IGLIIILAIM IYKKSATDVF EKHPCLYILM
310 320 330 340 350
FGCVFAKVSQ KLVVAHMTKS ELYLQDTVFL GPGLLFLDQY FNNFIDEYVV
360 370 380 390 400
LWMAMVISSF DMVIYFSALC LQISRHLHLN IFKTACHQAP EQVQVLSSKS

HQNNMD
Length:406
Mass (Da):45,097
Last modified:March 1, 2002 - v1
Checksum:iAE5357C02ED40E4C
GO
Isoform 2 (identifier: Q8WUD6-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     217-218: IP → FS
     219-406: Missing.

Show »
Length:218
Mass (Da):24,154
Checksum:i576CFC9B1E4F64F9
GO

Sequence cautioni

The sequence AAD44019 differs from that shown. Reason: Frameshift at position 310.Curated
The sequence AAL39005 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86P → L in AAD44019 (Ref. 5) Curated1
Sequence conflicti109S → P in AAD44019 (Ref. 5) Curated1
Sequence conflicti269L → P in AAD44019 (Ref. 5) Curated1
Sequence conflicti346D → G in AAD44019 (Ref. 5) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_032612162F → S1 PublicationCorresponds to variant dbSNP:rs3205421Ensembl.1
Natural variantiVAR_032613323Y → S in MCF-12A cell line. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_025989217 – 218IP → FS in isoform 2. 1 Publication2
Alternative sequenceiVSP_025990219 – 406Missing in isoform 2. 1 PublicationAdd BLAST188

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195623 mRNA Translation: AAF87947.1
AF195624 mRNA Translation: AAF87948.1
AK075211 mRNA Translation: BAG52084.1
CH471054 Genomic DNA Translation: EAW97675.1
BC020819 mRNA Translation: AAH20819.1
BC050429 mRNA Translation: AAH50429.1
AF047431 mRNA Translation: AAD44019.1 Frameshift.
AF111803 mRNA Translation: AAL39005.1 Different initiation.
AY280609 mRNA Translation: AAP34412.1
AY280610 mRNA Translation: AAP34413.1
AY294627 mRNA Translation: AAP37157.1
AY166717 mRNA Translation: AAN86122.1
AY166718 mRNA Translation: AAN86123.1
CCDSiCCDS9086.1 [Q8WUD6-1]
RefSeqiNP_064629.2, NM_020244.2 [Q8WUD6-1]
UniGeneiHs.293077

Genome annotation databases

EnsembliENST00000229266; ENSP00000229266; ENSG00000111666 [Q8WUD6-1]
ENST00000552351; ENSP00000447887; ENSG00000111666 [Q8WUD6-2]
GeneIDi56994
KEGGihsa:56994
UCSCiuc001tin.4 human [Q8WUD6-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCHPT1_HUMAN
AccessioniPrimary (citable) accession number: Q8WUD6
Secondary accession number(s): B3KQM2
, Q7Z7H0, Q7Z7H1, Q7Z7H2, Q8IWQ4, Q8IWQ5, Q8WYI4, Q9NRQ6, Q9NRQ7, Q9Y6M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: March 1, 2002
Last modified: April 25, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health