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Q8WUB8 (PHF10_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
PHD finger protein 10
Alternative name(s):
BRG1-associated factor 45a
Short name=BAF45a
XAP135
Gene names
Name:PHF10
Synonyms:BAF45A
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length498 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth By similarity.

Subunit structure

Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Interacts with ACTL6A/BAF53A, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A and PBRM1/BAF180 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the SAYP family.

Contains 2 PHD-type zinc fingers.

Caution

It is uncertain whether Met-1 or Met-89 is the initiator.

Sequence caution

The sequence AAH20954.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAI10324.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAK27451.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence BAA91934.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAD96332.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAG33554.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAI12316.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WUB8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WUB8-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     109-110: Missing.
Isoform 3 (identifier: Q8WUB8-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.12
Chain2 – 498497PHD finger protein 10
PRO_0000059297

Regions

Zinc finger379 – 43658PHD-type 1; degenerate
Zinc finger438 – 48144PHD-type 2; degenerate
Region89 – 295207SAY
Region89 – 18597Essential to induce neural progenitor proliferation By similarity
Region292 – 33443Essential to induce neural progenitor proliferation By similarity

Amino acid modifications

Modified residue21N-acetylalanine Ref.12
Modified residue121Phosphoserine Ref.12
Modified residue361Phosphoserine Ref.13
Modified residue501Phosphoserine Ref.13
Modified residue2701Phosphoserine Ref.9 Ref.12
Modified residue2971Phosphoserine Ref.9 Ref.11 Ref.12 Ref.13
Modified residue3011Phosphoserine Ref.9 Ref.11 Ref.12 Ref.13
Modified residue3271Phosphoserine Ref.11 Ref.13

Natural variations

Alternative sequence1 – 4747Missing in isoform 3.
VSP_039090
Alternative sequence109 – 1102Missing in isoform 2.
VSP_013440

Experimental info

Sequence conflict1261L → P in AAK27451. Ref.4
Sequence conflict2461E → G in AAK27451. Ref.4
Sequence conflict2751R → W in AAK27451. Ref.4
Sequence conflict286 – 2872PP → AT in AAK27451. Ref.4
Sequence conflict3251N → K in AAK27451. Ref.4
Sequence conflict3321P → S in AAK27451. Ref.4
Sequence conflict3571G → A in AAK27451. Ref.4
Sequence conflict3651K → N in AAK27451. Ref.4
Sequence conflict4221M → T in BAD96332. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 18, 2010. Version 3.
Checksum: 05EFDABD2000C2B7

FASTA49856,051
        10         20         30         40         50         60 
MAAAAGPGAA LSPRPCDSDP ATPGAQSPKD DNEDNSNDGT QPSKRRRMGS GDSSRSCETS 

        70         80         90        100        110        120 
SQDLGFSYYP AENLIEYKWP PDETGEYYML QEQVSEYLGV TSFKRKYPDL ERRDLSHKEK 

       130        140        150        160        170        180 
LYLRELNVIT ETQCTLGLTA LRSDEVIDLM IKEYPAKHAE YSVILQEKER QRITDHYKEY 

       190        200        210        220        230        240 
SQMQQQNTQK VEASKVPEYI KKAAKKAAEF NSNLNRERME ERRAYFDLQT HVIQVPQGKY 

       250        260        270        280        290        300 
KVLPTERTKV SSYPVALIPG QFQEYYKRYS PDELRYLPLN TALYEPPLDP ELPALDSDGD 

       310        320        330        340        350        360 
SDDGEDGRGD EKRKNKGTSD SSSGNVSEGE SPPDSQEDSF QGRQKSKDKA ATPRKDGPKR 

       370        380        390        400        410        420 
SVLSKSVPGY KPKVIPNAIC GICLKGKESN KKGKAESLIH CSQCENSGHP SCLDMTMELV 

       430        440        450        460        470        480 
SMIKTYPWQC MECKTCIICG QPHHEEEMMF CDMCDRGYHT FCVGLGAIPS GRWICDCCQR 

       490 
APPTPRKVGR RGKNSKEG 

« Hide

Isoform 2 (B) [UniParc].

Checksum: 6D4EAF7B86CA38C1
Show »

FASTA49655,823
Isoform 3 [UniParc].

Checksum: 8F3DE3D2A999E8A0
Show »

FASTA45151,290

References

« Hide 'large scale' references
[1]"PHF10 is required for cell proliferation in normal and SV40-immortalized human fibroblast cells."
Banga S.S., Peng L., Dasgupta T., Palejwala V., Ozer H.L.
Cytogenet. Genome Res. 126:227-242(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
[2]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Colon and Skin.
[4]"Physical and genetic characterization reveals a pseudogene, an evolutionary junction, and unstable loci in distal Xq28."
Aradhya S., Woffendin H., Bonnen P., Heiss N.S., Yamagata T., Esposito T., Bardaro T., Poustka A., D'Urso M., Kenwrick S., Nelson D.L.
Genomics 79:31-40(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 73-498 (ISOFORM 1), ALTERNATIVE SPLICING.
[5]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 87-498 (ISOFORM 1).
Tissue: Placenta.
[6]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 89-498 (ISOFORM 1).
[7]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 89-498 (ISOFORM 1).
Tissue: Artery smooth muscle.
[8]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270; SER-297 AND SER-301, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297; SER-301 AND SER-327, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-270; SER-297 AND SER-301, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36; SER-50; SER-297; SER-301 AND SER-327, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL513547 Genomic DNA. Translation: CAI12316.1. Sequence problems.
BC020954 mRNA. Translation: AAH20954.1. Different initiation.
BC110323 mRNA. Translation: AAI10324.1. Different initiation.
AF338735 Genomic DNA. Translation: AAK27451.1. Sequence problems.
AK001837 mRNA. Translation: BAA91934.1. Different initiation.
CR457273 mRNA. Translation: CAG33554.1. Different initiation.
AK222612 mRNA. Translation: BAD96332.1. Different initiation.
RefSeqNP_060758.2. NM_018288.3.
NP_579866.2. NM_133325.2.
UniGeneHs.435933.

3D structure databases

ProteinModelPortalQ8WUB8.
SMRQ8WUB8. Positions 371-479.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid120562. 21 interactions.
IntActQ8WUB8. 10 interactions.
MINTMINT-1373208.
STRING9606.ENSP00000341805.

PTM databases

PhosphoSiteQ8WUB8.

Polymorphism databases

DMDM296439276.

Proteomic databases

PaxDbQ8WUB8.
PRIDEQ8WUB8.

Protocols and materials databases

DNASU55274.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000339209; ENSP00000341805; ENSG00000130024. [Q8WUB8-1]
ENST00000366780; ENSP00000355743; ENSG00000130024. [Q8WUB8-2]
GeneID55274.
KEGGhsa:55274.
UCSCuc011egy.2. human. [Q8WUB8-1]
uc011egz.2. human. [Q8WUB8-2]

Organism-specific databases

CTD55274.
GeneCardsGC06M170104.
HGNCHGNC:18250. PHF10.
HPAHPA055649.
MIM613069. gene.
neXtProtNX_Q8WUB8.
PharmGKBPA134972675.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG279558.
HOGENOMHOG000286026.
HOVERGENHBG053581.
InParanoidQ8WUB8.
OMAEYKWPPD.
OrthoDBEOG72VH60.
PhylomeDBQ8WUB8.
TreeFamTF318971.

Gene expression databases

BgeeQ8WUB8.
CleanExHS_PHF10.
GenevestigatorQ8WUB8.

Family and domain databases

Gene3D3.30.40.10. 2 hits.
InterProIPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF00628. PHD. 1 hit.
[Graphical view]
SMARTSM00249. PHD. 2 hits.
[Graphical view]
SUPFAMSSF57903. SSF57903. 2 hits.
PROSITEPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPHF10.
GenomeRNAi55274.
NextBio59399.
PROQ8WUB8.
SOURCESearch...

Entry information

Entry namePHF10_HUMAN
AccessionPrimary (citable) accession number: Q8WUB8
Secondary accession number(s): Q2YDA3 expand/collapse secondary AC list , Q53HG8, Q9BXD2, Q9NV26
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 18, 2010
Last modified: April 16, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM