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Protein

PHD finger protein 10

Gene

PHF10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri379 – 436PHD-type 1; degeneratePROSITE-ProRule annotationAdd BLAST58
Zinc fingeri438 – 481PHD-type 2; degeneratePROSITE-ProRule annotationAdd BLAST44

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
PHD finger protein 10
Alternative name(s):
BRG1-associated factor 45a
Short name:
BAF45a
XAP135
Gene namesi
Name:PHF10
Synonyms:BAF45A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:18250. PHF10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55274.
OpenTargetsiENSG00000130024.
PharmGKBiPA134972675.

Polymorphism and mutation databases

BioMutaiPHF10.
DMDMi296439276.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000592972 – 498PHD finger protein 10Add BLAST497

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei12PhosphoserineCombined sources1
Modified residuei36PhosphoserineCombined sources1
Modified residuei50PhosphoserineCombined sources1
Cross-linki241Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei270PhosphoserineCombined sources1
Modified residuei297PhosphoserineCombined sources1
Modified residuei301PhosphoserineCombined sources1
Modified residuei327PhosphoserineCombined sources1
Modified residuei331PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ8WUB8.
MaxQBiQ8WUB8.
PaxDbiQ8WUB8.
PeptideAtlasiQ8WUB8.
PRIDEiQ8WUB8.

PTM databases

iPTMnetiQ8WUB8.
PhosphoSitePlusiQ8WUB8.

Expressioni

Gene expression databases

BgeeiENSG00000130024.
CleanExiHS_PHF10.
ExpressionAtlasiQ8WUB8. baseline and differential.
GenevisibleiQ8WUB8. HS.

Organism-specific databases

HPAiHPA055649.

Interactioni

Subunit structurei

Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Interacts with ACTL6A/BAF53A, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A and PBRM1/BAF180 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
L3MBTL2Q969R55EBI-10276329,EBI-739909

Protein-protein interaction databases

BioGridi120562. 33 interactors.
IntActiQ8WUB8. 11 interactors.
MINTiMINT-1373208.
STRINGi9606.ENSP00000341805.

Structurei

3D structure databases

ProteinModelPortaliQ8WUB8.
SMRiQ8WUB8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni89 – 295SAYAdd BLAST207
Regioni89 – 185Essential to induce neural progenitor proliferationBy similarityAdd BLAST97
Regioni292 – 334Essential to induce neural progenitor proliferationBy similarityAdd BLAST43

Sequence similaritiesi

Belongs to the SAYP family.Curated
Contains 2 PHD-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri379 – 436PHD-type 1; degeneratePROSITE-ProRule annotationAdd BLAST58
Zinc fingeri438 – 481PHD-type 2; degeneratePROSITE-ProRule annotationAdd BLAST44

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1512. Eukaryota.
ENOG410Y0WP. LUCA.
GeneTreeiENSGT00530000063194.
HOGENOMiHOG000286026.
HOVERGENiHBG053581.
InParanoidiQ8WUB8.
OMAiDSSRSCD.
OrthoDBiEOG091G0XBM.
PhylomeDBiQ8WUB8.
TreeFamiTF318971.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00628. PHD. 1 hit.
[Graphical view]
SMARTiSM00249. PHD. 2 hits.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 2 hits.
PROSITEiPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 2 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WUB8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAAGPGAA LSPRPCDSDP ATPGAQSPKD DNEDNSNDGT QPSKRRRMGS
60 70 80 90 100
GDSSRSCETS SQDLGFSYYP AENLIEYKWP PDETGEYYML QEQVSEYLGV
110 120 130 140 150
TSFKRKYPDL ERRDLSHKEK LYLRELNVIT ETQCTLGLTA LRSDEVIDLM
160 170 180 190 200
IKEYPAKHAE YSVILQEKER QRITDHYKEY SQMQQQNTQK VEASKVPEYI
210 220 230 240 250
KKAAKKAAEF NSNLNRERME ERRAYFDLQT HVIQVPQGKY KVLPTERTKV
260 270 280 290 300
SSYPVALIPG QFQEYYKRYS PDELRYLPLN TALYEPPLDP ELPALDSDGD
310 320 330 340 350
SDDGEDGRGD EKRKNKGTSD SSSGNVSEGE SPPDSQEDSF QGRQKSKDKA
360 370 380 390 400
ATPRKDGPKR SVLSKSVPGY KPKVIPNAIC GICLKGKESN KKGKAESLIH
410 420 430 440 450
CSQCENSGHP SCLDMTMELV SMIKTYPWQC MECKTCIICG QPHHEEEMMF
460 470 480 490
CDMCDRGYHT FCVGLGAIPS GRWICDCCQR APPTPRKVGR RGKNSKEG
Length:498
Mass (Da):56,051
Last modified:May 18, 2010 - v3
Checksum:i05EFDABD2000C2B7
GO
Isoform 2 (identifier: Q8WUB8-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     109-110: Missing.

Show »
Length:496
Mass (Da):55,823
Checksum:i6D4EAF7B86CA38C1
GO
Isoform 3 (identifier: Q8WUB8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.

Show »
Length:451
Mass (Da):51,290
Checksum:i8F3DE3D2A999E8A0
GO

Sequence cautioni

The sequence AAH20954 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAI10324 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAK27451 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAA91934 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAD96332 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAG33554 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence CAI12316 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126L → P in AAK27451 (PubMed:11827455).Curated1
Sequence conflicti246E → G in AAK27451 (PubMed:11827455).Curated1
Sequence conflicti275R → W in AAK27451 (PubMed:11827455).Curated1
Sequence conflicti286 – 287PP → AT in AAK27451 (PubMed:11827455).Curated2
Sequence conflicti325N → K in AAK27451 (PubMed:11827455).Curated1
Sequence conflicti332P → S in AAK27451 (PubMed:11827455).Curated1
Sequence conflicti357G → A in AAK27451 (PubMed:11827455).Curated1
Sequence conflicti365K → N in AAK27451 (PubMed:11827455).Curated1
Sequence conflicti422M → T in BAD96332 (Ref. 7) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0390901 – 47Missing in isoform 3. 1 PublicationAdd BLAST47
Alternative sequenceiVSP_013440109 – 110Missing in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513547 Genomic DNA. Translation: CAI12316.1. Sequence problems.
BC020954 mRNA. Translation: AAH20954.1. Different initiation.
BC110323 mRNA. Translation: AAI10324.1. Different initiation.
AF338735 Genomic DNA. Translation: AAK27451.1. Sequence problems.
AK001837 mRNA. Translation: BAA91934.1. Different initiation.
CR457273 mRNA. Translation: CAG33554.1. Different initiation.
AK222612 mRNA. Translation: BAD96332.1. Different initiation.
CCDSiCCDS5308.2. [Q8WUB8-1]
CCDS5309.2. [Q8WUB8-2]
RefSeqiNP_060758.2. NM_018288.3. [Q8WUB8-1]
NP_579866.2. NM_133325.2. [Q8WUB8-2]
UniGeneiHs.435933.

Genome annotation databases

EnsembliENST00000339209; ENSP00000341805; ENSG00000130024. [Q8WUB8-1]
ENST00000366780; ENSP00000355743; ENSG00000130024. [Q8WUB8-2]
ENST00000621772; ENSP00000484117; ENSG00000130024. [Q8WUB8-3]
GeneIDi55274.
KEGGihsa:55274.
UCSCiuc011egy.3. human. [Q8WUB8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL513547 Genomic DNA. Translation: CAI12316.1. Sequence problems.
BC020954 mRNA. Translation: AAH20954.1. Different initiation.
BC110323 mRNA. Translation: AAI10324.1. Different initiation.
AF338735 Genomic DNA. Translation: AAK27451.1. Sequence problems.
AK001837 mRNA. Translation: BAA91934.1. Different initiation.
CR457273 mRNA. Translation: CAG33554.1. Different initiation.
AK222612 mRNA. Translation: BAD96332.1. Different initiation.
CCDSiCCDS5308.2. [Q8WUB8-1]
CCDS5309.2. [Q8WUB8-2]
RefSeqiNP_060758.2. NM_018288.3. [Q8WUB8-1]
NP_579866.2. NM_133325.2. [Q8WUB8-2]
UniGeneiHs.435933.

3D structure databases

ProteinModelPortaliQ8WUB8.
SMRiQ8WUB8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120562. 33 interactors.
IntActiQ8WUB8. 11 interactors.
MINTiMINT-1373208.
STRINGi9606.ENSP00000341805.

PTM databases

iPTMnetiQ8WUB8.
PhosphoSitePlusiQ8WUB8.

Polymorphism and mutation databases

BioMutaiPHF10.
DMDMi296439276.

Proteomic databases

EPDiQ8WUB8.
MaxQBiQ8WUB8.
PaxDbiQ8WUB8.
PeptideAtlasiQ8WUB8.
PRIDEiQ8WUB8.

Protocols and materials databases

DNASUi55274.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000339209; ENSP00000341805; ENSG00000130024. [Q8WUB8-1]
ENST00000366780; ENSP00000355743; ENSG00000130024. [Q8WUB8-2]
ENST00000621772; ENSP00000484117; ENSG00000130024. [Q8WUB8-3]
GeneIDi55274.
KEGGihsa:55274.
UCSCiuc011egy.3. human. [Q8WUB8-1]

Organism-specific databases

CTDi55274.
DisGeNETi55274.
GeneCardsiPHF10.
HGNCiHGNC:18250. PHF10.
HPAiHPA055649.
MIMi613069. gene.
neXtProtiNX_Q8WUB8.
OpenTargetsiENSG00000130024.
PharmGKBiPA134972675.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1512. Eukaryota.
ENOG410Y0WP. LUCA.
GeneTreeiENSGT00530000063194.
HOGENOMiHOG000286026.
HOVERGENiHBG053581.
InParanoidiQ8WUB8.
OMAiDSSRSCD.
OrthoDBiEOG091G0XBM.
PhylomeDBiQ8WUB8.
TreeFamiTF318971.

Miscellaneous databases

GeneWikiiPHF10.
GenomeRNAii55274.
PROiQ8WUB8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000130024.
CleanExiHS_PHF10.
ExpressionAtlasiQ8WUB8. baseline and differential.
GenevisibleiQ8WUB8. HS.

Family and domain databases

Gene3Di3.30.40.10. 2 hits.
InterProiIPR011011. Znf_FYVE_PHD.
IPR001965. Znf_PHD.
IPR019787. Znf_PHD-finger.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF00628. PHD. 1 hit.
[Graphical view]
SMARTiSM00249. PHD. 2 hits.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 2 hits.
PROSITEiPS01359. ZF_PHD_1. 1 hit.
PS50016. ZF_PHD_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHF10_HUMAN
AccessioniPrimary (citable) accession number: Q8WUB8
Secondary accession number(s): Q2YDA3
, Q53HG8, Q9BXD2, Q9NV26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 18, 2010
Last modified: November 30, 2016
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-89 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.