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Protein

TBC1 domain family member 22A

Gene

TBC1D22A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a GTPase-activating protein for Rab family protein(s).By similarity

GO - Molecular functioni

  • 14-3-3 protein binding Source: UniProtKB
  • GTPase activator activity Source: GO_Central
  • protein homodimerization activity Source: UniProtKB
  • Rab GTPase binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
TBC1 domain family member 22A
Gene namesi
Name:TBC1D22A
Synonyms:C22orf4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:1309. TBC1D22A.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi90 – 923NSE → AAA: No effect on ACBD3-binding. 1 Publication
Mutagenesisi92 – 932EV → AA: No effect on interaction with ACBD3, nor with 14-3-3 proteins. 1 Publication
Mutagenesisi93 – 964VVME → AAAA: Decreased ACBD3-binding. 1 Publication
Mutagenesisi96 – 972ET → AA: No effect on ACBD3-binding. 1 Publication
Mutagenesisi99 – 1002NR → AA: No effect on ACBD3-binding. 1 Publication
Mutagenesisi101 – 1022VL → AA: Drastically decreased ACBD3-binding. No effect on binding to 14-3-3 protein. 1 Publication
Mutagenesisi103 – 1053RNH → AAA: No effect on ACBD3-binding. 1 Publication
Mutagenesisi132 – 1321S → A: No effect on 14-3-3 protein-binding. 1 Publication
Mutagenesisi149 – 1502TS → EE: Decreased 14-3-3 protein-binding. 1 Publication
Mutagenesisi165 – 1673SQS → AQA: Complete loss of 14-3-3 protein-binding. 1 Publication
Mutagenesisi165 – 1673SQS → EQE: Complete loss of 14-3-3 protein-binding. 1 Publication
Mutagenesisi165 – 1651S → A: No effect on interaction with ACBD3. Decreased 14-3-3 protein-binding. 1 Publication
Mutagenesisi165 – 1651S → E: Complete loss of 14-3-3 protein-binding. 1 Publication
Mutagenesisi167 – 1671S → E: Complete loss of 14-3-3 protein-binding. 1 Publication
Mutagenesisi320 – 3201Q → A: Decreased 14-3-3 protein-binding. 1 Publication

Organism-specific databases

PharmGKBiPA25888.

Polymorphism and mutation databases

BioMutaiTBC1D22A.
DMDMi25008319.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 517516TBC1 domain family member 22APRO_0000208051Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei132 – 1321PhosphoserineCombined sources
Modified residuei145 – 1451PhosphoserineBy similarity
Modified residuei167 – 1671PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8WUA7.
MaxQBiQ8WUA7.
PaxDbiQ8WUA7.
PeptideAtlasiQ8WUA7.
PRIDEiQ8WUA7.

PTM databases

iPTMnetiQ8WUA7.
PhosphoSiteiQ8WUA7.

Expressioni

Gene expression databases

BgeeiENSG00000054611.
CleanExiHS_TBC1D22A.
ExpressionAtlasiQ8WUA7. baseline and differential.
GenevisibleiQ8WUA7. HS.

Organism-specific databases

HPAiHPA001173.

Interactioni

Subunit structurei

Homodimer (PubMed:18186464). Interacts with ACBD3 and ARFGEF1. Interacts with YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ and YWHAZ (PubMed:23572552).2 Publications

GO - Molecular functioni

  • 14-3-3 protein binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • Rab GTPase binding Source: GO_Central

Protein-protein interaction databases

BioGridi117308. 23 interactions.
IntActiQ8WUA7. 3 interactions.
STRINGi9606.ENSP00000336724.

Structurei

Secondary structure

1
517
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi197 – 20610Combined sources
Helixi212 – 2198Combined sources
Helixi225 – 2273Combined sources
Helixi228 – 2358Combined sources
Helixi243 – 2453Combined sources
Helixi246 – 26318Combined sources
Helixi274 – 28310Combined sources
Helixi284 – 2863Combined sources
Helixi290 – 2934Combined sources
Helixi296 – 31217Combined sources
Turni314 – 3163Combined sources
Helixi322 – 33413Combined sources
Helixi335 – 3373Combined sources
Helixi353 – 37220Combined sources
Helixi375 – 3773Combined sources
Helixi383 – 39917Combined sources
Helixi401 – 4099Combined sources
Helixi414 – 42310Combined sources
Turni424 – 4296Combined sources
Helixi432 – 44211Combined sources
Turni446 – 4516Combined sources
Helixi452 – 46312Combined sources
Helixi465 – 4706Combined sources
Helixi474 – 4818Combined sources
Helixi491 – 50717Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QFZX-ray2.10A/B191-517[»]
ProteinModelPortaliQ8WUA7.
SMRiQ8WUA7. Positions 196-508.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WUA7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini222 – 446225Rab-GAP TBCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1092. Eukaryota.
ENOG410XQ68. LUCA.
GeneTreeiENSGT00840000129806.
HOGENOMiHOG000162526.
HOVERGENiHBG057030.
InParanoidiQ8WUA7.
OMAiFICEYIE.
OrthoDBiEOG091G055X.
PhylomeDBiQ8WUA7.
TreeFamiTF314211.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WUA7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASDGARKQF WKRSNSKLPG SIQHVYGAQH PPFDPLLHGT LLRSTAKMPT
60 70 80 90 100
TPVKAKRVST FQEFESNTSD AWDAGEDDDE LLAMAAESLN SEVVMETANR
110 120 130 140 150
VLRNHSQRQG RPTLQEGPGL QQKPRPEAEP PSPPSGDLRL VKSVSESHTS
160 170 180 190 200
CPAESASDAA PLQRSQSLPH SATVTLGGTS DPSTLSSSAL SEREASRLDK
210 220 230 240 250
FKQLLAGPNT DLEELRRLSW SGIPKPVRPM TWKLLSGYLP ANVDRRPATL
260 270 280 290 300
QRKQKEYFAF IEHYYDSRND EVHQDTYRQI HIDIPRMSPE ALILQPKVTE
310 320 330 340 350
IFERILFIWA IRHPASGYVQ GINDLVTPFF VVFICEYIEA EEVDTVDVSG
360 370 380 390 400
VPAEVLCNIE ADTYWCMSKL LDGIQDNYTF AQPGIQMKVK MLEELVSRID
410 420 430 440 450
EQVHRHLDQH EVRYLQFAFR WMNNLLMREV PLRCTIRLWD TYQSEPDGFS
460 470 480 490 500
HFHLYVCAAF LVRWRKEILE EKDFQELLLF LQNLPTAHWD DEDISLLLAE
510
AYRLKFAFAD APNHYKK
Length:517
Mass (Da):59,121
Last modified:November 8, 2002 - v2
Checksum:i865A3CDCDA0C5543
GO
Isoform 2 (identifier: Q8WUA7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     22-40: Missing.
     154-212: Missing.

Note: No experimental confirmation available.
Show »
Length:439
Mass (Da):50,931
Checksum:i6B0974FFA893D264
GO
Isoform 3 (identifier: Q8WUA7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.
     339-371: EAEEVDTVDVSGVPAEVLCNIEADTYWCMSKLL → AFPGCGRPQIPILAVIWRDEPYPRTDEQIILRR
     372-517: Missing.

Note: No experimental confirmation available.
Show »
Length:324
Mass (Da):36,514
Checksum:i3FB78F85F1CDEDF3
GO
Isoform 4 (identifier: Q8WUA7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-47: Missing.

Note: No experimental confirmation available.
Show »
Length:470
Mass (Da):53,908
Checksum:iE6E52DB6BB248975
GO

Sequence cautioni

The sequence CAB46628 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti92 – 921E → K in AAH01292 (PubMed:15489334).Curated
Sequence conflicti339 – 3457EAEEVDT → GKISCKH in AAD12228 (PubMed:10591208).Curated
Sequence conflicti350 – 3501G → C in AAH01292 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4747Missing in isoform 3 and isoform 4. 1 PublicationVSP_015142Add
BLAST
Alternative sequencei22 – 4019Missing in isoform 2. 1 PublicationVSP_010382Add
BLAST
Alternative sequencei154 – 21259Missing in isoform 2. 1 PublicationVSP_010383Add
BLAST
Alternative sequencei339 – 37133EAEEV…MSKLL → AFPGCGRPQIPILAVIWRDE PYPRTDEQIILRR in isoform 3. 1 PublicationVSP_015143Add
BLAST
Alternative sequencei372 – 517146Missing in isoform 3. 1 PublicationVSP_015144Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB449908 mRNA. Translation: BAH16651.1.
CR456412 mRNA. Translation: CAG30298.1.
AK125705 mRNA. Translation: BAC86253.1.
AK127653 mRNA. Translation: BAG54543.1.
U51561 Genomic DNA. Translation: AAD12228.1.
AL118516
, AL023733, FP325331, U51561, Z79999, Z80896, Z82187 Genomic DNA. Translation: CAI17954.1.
AL023733
, FP325331, AL118516, U51561, Z79999, Z80896, Z82187 Genomic DNA. Translation: CAI18761.1.
Z80896
, AL023733, FP325331, AL118516, U51561, Z79999, Z82187 Genomic DNA. Translation: CAI22357.1.
Z79999
, AL023733, FP325331, AL118516, U51561, Z80896, Z82187 Genomic DNA. Translation: CAI23583.1.
Z82187
, AL023733, FP325331, AL118516, U51561, Z80896, Z79999 Genomic DNA. Translation: CAI23588.1.
Z79999
, AL023733, FP325331, AL118516, U51561, Z80896, Z82187 Genomic DNA. Translation: CAQ07922.1.
Z80896
, AL023733, FP325331, AL118516, U51561, Z79999, Z82187 Genomic DNA. Translation: CAQ09326.1.
Z82187
, AL023733, FP325331, AL118516, U51561, Z79999, Z80896 Genomic DNA. Translation: CAQ09531.1.
AL118516
, AL023733, FP325331, U51561, Z79999, Z80896, Z82187 Genomic DNA. Translation: CAQ10969.1.
AL023733
, FP325331, AL118516, U51561, Z79999, Z80896, Z82187 Genomic DNA. Translation: CAQ11024.1.
CH471138 Genomic DNA. Translation: EAW73443.1.
CH471138 Genomic DNA. Translation: EAW73445.1.
BC001292 mRNA. Translation: AAH01292.2.
BC002743 mRNA. Translation: AAH02743.2.
BC020976 mRNA. Translation: AAH20976.2.
BC029897 mRNA. Translation: AAH29897.1.
AL096779 mRNA. Translation: CAB46628.1. Different initiation.
CCDSiCCDS14078.1. [Q8WUA7-1]
CCDS63511.1. [Q8WUA7-2]
CCDS63512.1. [Q8WUA7-4]
RefSeqiNP_001271232.1. NM_001284303.1. [Q8WUA7-2]
NP_001271234.1. NM_001284305.1. [Q8WUA7-4]
NP_055161.1. NM_014346.3. [Q8WUA7-1]
UniGeneiHs.435044.

Genome annotation databases

EnsembliENST00000337137; ENSP00000336724; ENSG00000054611. [Q8WUA7-1]
ENST00000355704; ENSP00000347932; ENSG00000054611. [Q8WUA7-2]
ENST00000406733; ENSP00000385634; ENSG00000054611. [Q8WUA7-4]
ENST00000441162; ENSP00000406214; ENSG00000054611. [Q8WUA7-3]
GeneIDi25771.
KEGGihsa:25771.
UCSCiuc003bib.5. human. [Q8WUA7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB449908 mRNA. Translation: BAH16651.1.
CR456412 mRNA. Translation: CAG30298.1.
AK125705 mRNA. Translation: BAC86253.1.
AK127653 mRNA. Translation: BAG54543.1.
U51561 Genomic DNA. Translation: AAD12228.1.
AL118516
, AL023733, FP325331, U51561, Z79999, Z80896, Z82187 Genomic DNA. Translation: CAI17954.1.
AL023733
, FP325331, AL118516, U51561, Z79999, Z80896, Z82187 Genomic DNA. Translation: CAI18761.1.
Z80896
, AL023733, FP325331, AL118516, U51561, Z79999, Z82187 Genomic DNA. Translation: CAI22357.1.
Z79999
, AL023733, FP325331, AL118516, U51561, Z80896, Z82187 Genomic DNA. Translation: CAI23583.1.
Z82187
, AL023733, FP325331, AL118516, U51561, Z80896, Z79999 Genomic DNA. Translation: CAI23588.1.
Z79999
, AL023733, FP325331, AL118516, U51561, Z80896, Z82187 Genomic DNA. Translation: CAQ07922.1.
Z80896
, AL023733, FP325331, AL118516, U51561, Z79999, Z82187 Genomic DNA. Translation: CAQ09326.1.
Z82187
, AL023733, FP325331, AL118516, U51561, Z79999, Z80896 Genomic DNA. Translation: CAQ09531.1.
AL118516
, AL023733, FP325331, U51561, Z79999, Z80896, Z82187 Genomic DNA. Translation: CAQ10969.1.
AL023733
, FP325331, AL118516, U51561, Z79999, Z80896, Z82187 Genomic DNA. Translation: CAQ11024.1.
CH471138 Genomic DNA. Translation: EAW73443.1.
CH471138 Genomic DNA. Translation: EAW73445.1.
BC001292 mRNA. Translation: AAH01292.2.
BC002743 mRNA. Translation: AAH02743.2.
BC020976 mRNA. Translation: AAH20976.2.
BC029897 mRNA. Translation: AAH29897.1.
AL096779 mRNA. Translation: CAB46628.1. Different initiation.
CCDSiCCDS14078.1. [Q8WUA7-1]
CCDS63511.1. [Q8WUA7-2]
CCDS63512.1. [Q8WUA7-4]
RefSeqiNP_001271232.1. NM_001284303.1. [Q8WUA7-2]
NP_001271234.1. NM_001284305.1. [Q8WUA7-4]
NP_055161.1. NM_014346.3. [Q8WUA7-1]
UniGeneiHs.435044.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QFZX-ray2.10A/B191-517[»]
ProteinModelPortaliQ8WUA7.
SMRiQ8WUA7. Positions 196-508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117308. 23 interactions.
IntActiQ8WUA7. 3 interactions.
STRINGi9606.ENSP00000336724.

PTM databases

iPTMnetiQ8WUA7.
PhosphoSiteiQ8WUA7.

Polymorphism and mutation databases

BioMutaiTBC1D22A.
DMDMi25008319.

Proteomic databases

EPDiQ8WUA7.
MaxQBiQ8WUA7.
PaxDbiQ8WUA7.
PeptideAtlasiQ8WUA7.
PRIDEiQ8WUA7.

Protocols and materials databases

DNASUi25771.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337137; ENSP00000336724; ENSG00000054611. [Q8WUA7-1]
ENST00000355704; ENSP00000347932; ENSG00000054611. [Q8WUA7-2]
ENST00000406733; ENSP00000385634; ENSG00000054611. [Q8WUA7-4]
ENST00000441162; ENSP00000406214; ENSG00000054611. [Q8WUA7-3]
GeneIDi25771.
KEGGihsa:25771.
UCSCiuc003bib.5. human. [Q8WUA7-1]

Organism-specific databases

CTDi25771.
GeneCardsiTBC1D22A.
H-InvDBHIX0022662.
HGNCiHGNC:1309. TBC1D22A.
HPAiHPA001173.
MIMi616879. gene.
neXtProtiNX_Q8WUA7.
PharmGKBiPA25888.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1092. Eukaryota.
ENOG410XQ68. LUCA.
GeneTreeiENSGT00840000129806.
HOGENOMiHOG000162526.
HOVERGENiHBG057030.
InParanoidiQ8WUA7.
OMAiFICEYIE.
OrthoDBiEOG091G055X.
PhylomeDBiQ8WUA7.
TreeFamiTF314211.

Miscellaneous databases

ChiTaRSiTBC1D22A. human.
EvolutionaryTraceiQ8WUA7.
GenomeRNAii25771.
PROiQ8WUA7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000054611.
CleanExiHS_TBC1D22A.
ExpressionAtlasiQ8WUA7. baseline and differential.
GenevisibleiQ8WUA7. HS.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTB22A_HUMAN
AccessioniPrimary (citable) accession number: Q8WUA7
Secondary accession number(s): B0QYI2
, B0QYI3, B9A6M3, Q5TE47, Q6ZUH2, Q92680, Q9BVD6, Q9UGG0, Q9UGT2, Q9UGU6, Q9UH25, Q9Y4W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: September 7, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.