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Q8WUA4 (TF3C2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
General transcription factor 3C polypeptide 2
Alternative name(s):
TF3C-beta
Transcription factor IIIC 110 kDa subunit
Short name=TFIIIC 110 kDa subunit
Short name=TFIIIC110
Transcription factor IIIC subunit beta
Gene names
Name:GTF3C2
Synonyms:KIAA0011
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length911 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.

Subunit structure

Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Ref.1

Subcellular location

Nucleus.

Sequence similarities

Contains 4 WD repeats.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WUA4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8WUA4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     579-585: MVVFWNL → KKNQNKT
     586-911: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 911911General transcription factor 3C polypeptide 2
PRO_0000050985

Regions

Repeat461 – 51757WD 1
Repeat548 – 58942WD 2
Repeat607 – 64741WD 3
Repeat828 – 87043WD 4
Compositional bias151 – 1566Poly-Leu

Amino acid modifications

Modified residue631Phosphoserine Ref.10
Modified residue1321Phosphoserine Ref.9
Modified residue1651Phosphoserine Ref.11
Modified residue1671Phosphoserine Ref.9 Ref.11 Ref.13
Modified residue2201Phosphoserine Ref.13
Modified residue8711Phosphoserine Ref.11
Modified residue8921Phosphoserine Ref.11 Ref.13
Modified residue8931Phosphoserine Ref.11
Modified residue8951Phosphothreonine Ref.10

Natural variations

Alternative sequence579 – 5857MVVFWNL → KKNQNKT in isoform 2.
VSP_010566
Alternative sequence586 – 911326Missing in isoform 2.
VSP_010567

Experimental info

Sequence conflict3011N → D in AAP88801. Ref.3
Sequence conflict3011N → D in AAH20981. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 7, 2004. Version 2.
Checksum: B59D91DE74555192

FASTA911100,680
        10         20         30         40         50         60 
MDTCGVGYVA LGEAGPVGNM TVVDSPGQEV LNQLDVKTSS EMTSAEASVE MSLPTPLPGF 

        70         80         90        100        110        120 
EDSPDQRRLP PEQESLSRLE QPDLSSEMSK VSKPRASKPG RKRGGRTRKG PKRPQQPNPP 

       130        140        150        160        170        180 
SAPLVPGLLD QSNPLSTPMP KKRGRKSKAE LLLLKLSKDL DRPESQSPKR PPEDFETPSG 

       190        200        210        220        230        240 
ERPRRRAAQV ALLYLQELAE ELSTALPAPV SCPEGPKVSS PTKPKKIRQP AACPGGEEVD 

       250        260        270        280        290        300 
GAPRDEDFFL QVEAEDVEES EGPSESSSEP EPVVPRSTPR GSTSGKQKPH CRGMAPNGLP 

       310        320        330        340        350        360 
NHIMAPVWKC LHLTKDFREQ KHSYWEFAEW IPLAWKWHLL SELEAAPYLP QEEKSPLFSV 

       370        380        390        400        410        420 
QREGLPEDGT LYRINRFSSI TAHPERWDVS FFTGGPLWAL DWCPVPEGAG ASQYVALFSS 

       430        440        450        460        470        480 
PDMNETHPLS QLHSGPGLLQ LWGLGTLQQE SCPGNRAHFV YGIACDNGCI WDLKFCPSGA 

       490        500        510        520        530        540 
WELPGTPRKA PLLPRLGLLA LACSDGKVLL FSLPHPEALL AQQPPDAVKP AIYKVQCVAT 

       550        560        570        580        590        600 
LQVGSMQATD PSECGQCLSL AWMPTRPHQH LAAGYYNGMV VFWNLPTNSP LQRIRLSDGS 

       610        620        630        640        650        660 
LKLYPFQCFL AHDQAVRTLQ WCKANSHFLV SAGSDRKIKF WDLRRPYEPI NSIKRFLSTE 

       670        680        690        700        710        720 
LAWLLPYNGV TVAQDNCYAS YGLCGIHYID AGYLGFKAYF TAPRKGTVWS LSGSDWLGTI 

       730        740        750        760        770        780 
AAGDISGELI AAILPDMALN PINVKRPVER RFPIYKADLI PYQDSPEGPD HSSASSGVPN 

       790        800        810        820        830        840 
PPKARTYTET VNHHYLLFQD TDLGSFHDLL RREPMLRMQE GEGHSQLCLD RLQLEAIHKV 

       850        860        870        880        890        900 
RFSPNLDSYG WLVSGGQSGL VRIHFVRGLA SPLGHRMQLE SRAHFNAMFQ PSSPTRRPGF 

       910 
SPTSHRLLPT P 

« Hide

Isoform 2 [UniParc].

Checksum: 0CBDAE93F3E08D6B
Show »

FASTA58564,003

References

« Hide 'large scale' references
[1]"Cloning and characterization of a TFIIIC2 subunit (TFIIIC beta) whose presence correlates with activation of RNA polymerase III-mediated transcription by adenovirus E1A expression and serum factors."
Sinn E., Wang Z., Kovelman R., Roeder R.G.
Genes Dev. 9:675-685(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 41-62; 338-354; 642-654 AND 757-774, IDENTIFICATION OF SUBUNITS OF TFIIIC2 COMPLEX.
[2]"Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1."
Nomura N., Miyajima N., Sazuka T., Tanaka A., Kawarabayasi Y., Sato S., Nagase T., Seki N., Ishikawa K., Tabata S.
DNA Res. 1:27-35(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Bone marrow.
[3]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain and Colon.
[6]Yu W., Gibbs R.A.
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 620-911.
Tissue: Brain.
[7]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[8]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132 AND SER-167, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63 AND THR-895, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[11]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-165; SER-167; SER-871; SER-892 AND SER-893, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[12]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167; SER-220 AND SER-892, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13636 mRNA. Translation: BAA02800.1.
BT009799 mRNA. Translation: AAP88801.1.
CH471053 Genomic DNA. Translation: EAX00595.1.
CH471053 Genomic DNA. Translation: EAX00596.1.
BC000212 mRNA. Translation: AAH00212.1.
BC020981 mRNA. Translation: AAH20981.1.
AF054988 mRNA. Translation: AAC09349.1.
CCDSCCDS1749.1. [Q8WUA4-1]
PIRA56465.
RefSeqNP_001030598.1. NM_001035521.2. [Q8WUA4-1]
NP_001512.1. NM_001521.3. [Q8WUA4-1]
UniGeneHs.745168.
Hs.75782.

3D structure databases

ProteinModelPortalQ8WUA4.
SMRQ8WUA4. Positions 834-869.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109231. 18 interactions.
DIPDIP-38213N.
IntActQ8WUA4. 8 interactions.
MINTMINT-4535724.
STRING9606.ENSP00000264720.

PTM databases

PhosphoSiteQ8WUA4.

Polymorphism databases

DMDM48428661.

Proteomic databases

MaxQBQ8WUA4.
PaxDbQ8WUA4.
PRIDEQ8WUA4.

Protocols and materials databases

DNASU2976.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264720; ENSP00000264720; ENSG00000115207. [Q8WUA4-1]
ENST00000359541; ENSP00000352536; ENSG00000115207. [Q8WUA4-1]
GeneID2976.
KEGGhsa:2976.
UCSCuc002rju.2. human. [Q8WUA4-1]
uc010eyz.3. human. [Q8WUA4-2]

Organism-specific databases

CTD2976.
GeneCardsGC02M027548.
HGNCHGNC:4665. GTF3C2.
HPAHPA035836.
MIM604883. gene.
neXtProtNX_Q8WUA4.
PharmGKBPA29053.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG44594.
HOGENOMHOG000133051.
HOVERGENHBG056791.
InParanoidQ8WUA4.
KOK15200.
OMARRFPIYK.
OrthoDBEOG7CNZF7.
PhylomeDBQ8WUA4.
TreeFamTF314779.

Enzyme and pathway databases

ReactomeREACT_1788. Transcription.
REACT_71. Gene Expression.
SignaLinkQ8WUA4.

Gene expression databases

ArrayExpressQ8WUA4.
BgeeQ8WUA4.
CleanExHS_GTF3C2.
GenevestigatorQ8WUA4.

Family and domain databases

Gene3D2.130.10.10. 2 hits.
InterProIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF00400. WD40. 1 hit.
[Graphical view]
SMARTSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 3 hits.
PROSITEPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGTF3C2. human.
GeneWikiGTF3C2.
GenomeRNAi2976.
NextBio11802.
PROQ8WUA4.
SOURCESearch...

Entry information

Entry nameTF3C2_HUMAN
AccessionPrimary (citable) accession number: Q8WUA4
Secondary accession number(s): D6W557, Q16632, Q9BWI7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: July 9, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM