Q8WU90 (ZC3HF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger CCCH domain-containing protein 15

Alternative name(s):
DRG family-regulatory protein 1
Likely ortholog of mouse immediate early response erythropoietin 4

Gene names

Name:	ZC3H15
Synonyms:	DFRP1, LEREPO4
ORF Names:	HSPC303, HT010, MSTP012, PP730

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protects DRG1 from proteolytic degradation By similarity.
Subunit structure	Interacts with DRG1; the interaction forms a polysomal DRG1-DFRP1/ZC3H15 complex which provides protein stability to DRG1 possibly by blocking poly-ubiquitination. Ref.9 Ref.11
Subcellular location	Cytoplasm. Nucleus By similarity. Note: The DRG1-DFRP2/ZC3H15 complex associates with polysomes. Ref.9 Ref.11
Sequence similarities	Belongs to the ZC3H15/TMA46 family. Contains 2 C3H1-type zinc fingers.
Sequence caution	The sequence AAF28981.1 differs from that shown. Reason: Frameshift at position 17. The sequence AAF67649.1 differs from that shown. Reason: Frameshift at positions 392, 407 and 422. The sequence AAL55770.1 differs from that shown. Reason: Frameshift at position 92. The sequence AAQ13514.1 differs from that shown. Reason: Frameshift at positions 404 and 424.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Domain	Coiled coil Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay. Source: HPA nucleolus Inferred from direct assay. Source: HPA
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	Zinc finger CCCH domain-containing protein 15		PRO_0000324642
Regions
	Zinc finger	99 – 126	28	C3H1-type 1
	Zinc finger	174 – 212	39	C3H1-type 2
	Region	236 – 260	25	Required for interaction with DRG1 By similarity
	Coiled coil	61 – 86	26	Potential
	Coiled coil	218 – 285	68	Potential
Amino acid modifications
	Modified residue	351	1	Phosphoserine Ref.12
	Modified residue	381	1	Phosphoserine Ref.10
Natural variations
	Natural variant	342	1	V → E. Corresponds to variant rs11555006 [ dbSNP | Ensembl ].		VAR_039867
	Natural variant	408	1	T → P. Corresponds to variant rs1043497 [ dbSNP | Ensembl ].		VAR_052969
Experimental info
	Sequence conflict	10	1	G → R in AAL55770. Ref.5
	Sequence conflict	16 – 17	2	EQ → DE in AAF67649. Ref.3
	Sequence conflict	17	1	Q → T in AAF28981. Ref.2
	Sequence conflict	26	1	D → G in AAL55770. Ref.5
	Sequence conflict	37	1	A → T in AAL55770. Ref.5
	Sequence conflict	77	1	E → D in AAF28981. Ref.2
	Sequence conflict	77	1	E → D in AAF67649. Ref.3
	Sequence conflict	112	1	Q → R in BAA91968. Ref.4
	Sequence conflict	342	1	V → A in AAF28981. Ref.2
	Sequence conflict	365	1	N → T in AAF28981. Ref.2
	Sequence conflict	413	1	D → V in AAQ13514. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8WU90 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 4A80B7FDC3376181	FASTA	426	48,602
	10 20 30 40 50 60 MPPKKQAQAG GSKKAEQKKK EKIIEDKTFG LKNKKGAKQQ KFIKAVTHQV KFGQQNPRQV 70 80 90 100 110 120 AQSEAEKKLK KDDKKKELQE LNELFKPVVA AQKISKGADP KSVVCAFFKQ GQCTKGDKCK 130 140 150 160 170 180 FSHDLTLERK CEKRSVYIDA RDEELEKDTM DNWDEKKLEE VVNKKHGEAE KKKPKTQIVC 190 200 210 220 230 240 KHFLEAIENN KYGWFWVCPG GGDICMYRHA LPPGFVLKKD KKKEEKEDEI SLEDLIERER 250 260 270 280 290 300 SALGPNVTKI TLESFLAWKK RKRQEKIDKL EQDMERRKAD FKAGKALVIS GREVFEFRPE 310 320 330 340 350 360 LVNDDDEEAD DTRYTQGTGG DEVDDSVSVN DIDLSLYIPR DVDETGITVA SLERFSTYTS 370 380 390 400 410 420 DKDENKLSEA SGGRAENGER SDLEEDNERE GTENGAIDAV PVDENLFTGE DLDELEEELN TLDLEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hui R.T., Liu Y.Q., Liu B., Zhao B., Meng X.M., Sheng H., Xu Y.Y., Wang X.Y., Ye J., Song L., Gao Y., Wei Y.J., Zhang C.L., Zhang J., Chai M.Q., Chen J.Z., Sun Y.H., Zhou X.L. , Jiang Y.X., Zhao X.W., Liu S., Cao H.Q., Zhao Y., Liu D.Q., Ding J.F., Liu L.S., Gao R.L., Wu Q.Y., Qiang B.Q., Yuan J.G., Liew C.C., Zhao M.S. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Aorta.
[2]	"Human partial CDS from CD34+ stem cells." Ye M., Zhang Q.-H., Zhou J., Shen Y., Wu X.-Y., Guan Z.Q., Wang L., Fan H.-Y., Mao Y.-F., Dai M., Huang Q.-H., Chen S.-J., Chen Z. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Umbilical cord blood.
[3]	"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M. , Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L. Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed: 10931946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Hypothalamus.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[5]	"Large-scale cDNA transfection screening for genes related to cancer development and progression." Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X., Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X. , Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J. Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed: 15498874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[9]	"Identification of DRG family regulatory proteins (DFRPs): specific regulation of DRG1 and DRG2." Ishikawa K., Azuma S., Ikawa S., Semba K., Inoue J. Genes Cells 10:139-150(2005) [PubMed: 15676025] [Abstract] Cited for: INTERACTION WITH DRG1, SUBCELLULAR LOCATION.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Independent stabilizations of polysomal Drg1/Dfrp1 complex and non-polysomal Drg2/Dfrp2 complex in mammalian cells." Ishikawa K., Akiyama T., Ito K., Semba K., Inoue J. Biochem. Biophys. Res. Commun. 390:552-556(2009) [PubMed: 19819225] [Abstract] Cited for: INTERACTION WITH DRG1 IN THE DRG1-DFRP1/ZC3H15 COMPLEX, SUBCELLULAR LOCATION.
[12]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[13]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF109366 mRNA. Translation: AAQ13514.1. Frameshift. AF161421 mRNA. Translation: AAF28981.1. Frameshift. AF220184 mRNA. Translation: AAF67649.1. Frameshift. AK001901 mRNA. Translation: BAA91968.1. AF289586 mRNA. Translation: AAL55770.1. Frameshift. AC018867 Genomic DNA. Translation: AAX88885.1. CH471058 Genomic DNA. Translation: EAX10935.1. CH471058 Genomic DNA. Translation: EAX10936.1. BC021102 mRNA. Translation: AAH21102.1.
IPI	IPI00000279.
RefSeq	NP_060941.2. NM_018471.2.
UniGene	Hs.725998.
3D structure databases
ProteinModelPortal	Q8WU90.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8WU90.
PTM databases
PhosphoSite	Q8WU90.
Polymorphism databases
DMDM	74730681.
Proteomic databases
PRIDE	Q8WU90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000337859; ENSP00000338788; ENSG00000065548.
GeneID	55854.
KEGG	hsa:55854.
UCSC	uc002upo.1. human.
Organism-specific databases
CTD	55854.
GeneCards	GC02P187350.
H-InvDB	HIX0002656.
HGNC	HGNC:29528. ZC3H15.
HPA	HPA031099. HPA031100. HPA031101.
neXtProt	NX_Q8WU90.
PharmGKB	PA162409508.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19745.
GeneTree	ENSGT00390000015818.
HOGENOM	HBG737197.
HOVERGEN	HBG108761.
InParanoid	Q8WU90.
OMA	EKDTMEN.
OrthoDB	EOG49S672.
PhylomeDB	Q8WU90.
Gene expression databases
ArrayExpress	Q8WU90.
Bgee	Q8WU90.
CleanEx	HS_ZC3H15.
Genevestigator	Q8WU90.
Family and domain databases
InterPro	IPR000571. Znf_CCCH. [Graphical view]
Pfam	PF00642. zf-CCCH. 1 hit. [Graphical view]
SMART	SM00356. ZnF_C3H1. 2 hits. [Graphical view]
PROSITE	PS50103. ZF_C3H1. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	61127.

Entry information

Entry name

ZC3HF_HUMAN

Accession

Primary (citable) accession number: Q8WU90
Secondary accession number(s): D3DPG7 , Q5QTQ4, Q8WZ06, Q9NUZ3, Q9NZ37, Q9P079

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.