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Protein

Phospholipase ABHD3

Gene

ABHD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phospholipase that may play a role in phospholipids remodeling. May selectively cleave myristate (C14)-containing phosphatidylcholines through its predominant phospholipase 1 activity, cleaving preferentially acyl groups in sn1 position. In parallel, may have a minor phospholipase 2 activity acting on acyl groups in position sn2. In addition to (C14)-containing phosphatidylcholines, may also act on other medium-chain-containing and oxidatively truncated phospholipids.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.1 Publication
Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei220 – 2201Charge relay systemBy similarity
Active sitei346 – 3461Charge relay systemBy similarity
Active sitei375 – 3751Charge relay systemBy similarity

GO - Molecular functioni

  1. 1-acyl-2-lysophosphatidylserine acylhydrolase activity Source: UniProtKB-EC
  2. phosphatidylcholine 1-acylhydrolase activity Source: UniProtKB
  3. phosphatidylserine 1-acylhydrolase activity Source: UniProtKB-EC
  4. phospholipase A2 activity Source: UniProtKB

GO - Biological processi

  1. phosphatidylcholine metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Lipid metabolism, Phospholipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase ABHD3Curated (EC:3.1.1.321 Publication, EC:3.1.1.41 Publication)
Alternative name(s):
Abhydrolase domain-containing protein 3Curated
Gene namesi
Name:ABHD3Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:18718. ABHD3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei26 – 4621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi220 – 2201S → A: Loss of phospholipase activity. 1 Publication

Organism-specific databases

PharmGKBiPA38659.

Polymorphism and mutation databases

BioMutaiABHD3.
DMDMi134035377.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 409409Phospholipase ABHD3PRO_0000280208Add
BLAST

Proteomic databases

MaxQBiQ8WU67.
PaxDbiQ8WU67.
PRIDEiQ8WU67.

PTM databases

PhosphoSiteiQ8WU67.

Expressioni

Gene expression databases

BgeeiQ8WU67.
CleanExiHS_ABHD3.
ExpressionAtlasiQ8WU67. baseline and differential.
GenevestigatoriQ8WU67.

Organism-specific databases

HPAiHPA010729.
HPA052862.

Interactioni

Protein-protein interaction databases

BioGridi128149. 5 interactions.
IntActiQ8WU67. 1 interaction.
STRINGi9606.ENSP00000289119.

Structurei

3D structure databases

ProteinModelPortaliQ8WU67.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0429.
GeneTreeiENSGT00530000062981.
HOGENOMiHOG000230596.
HOVERGENiHBG053994.
InParanoidiQ8WU67.
KOiK13696.
OMAiFITSKPQ.
OrthoDBiEOG70PBXF.
PhylomeDBiQ8WU67.
TreeFamiTF313195.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR012020. AB-Hydro_YheT.
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000952. UPF0017_hydro-like_CS.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF005211. Ab_hydro_YheT. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01133. UPF0017. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WU67-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQRLAMDLRM LSRELSLYLE HQVRVGFFGS GVGLSLILGF SVAYAFYYLS
60 70 80 90 100
SIAKKPQLVT GGESFSRFLQ DHCPVVTETY YPTVWCWEGR GQTLLRPFIT
110 120 130 140 150
SKPPVQYRNE LIKTADGGQI SLDWFDNDNS TCYMDASTRP TILLLPGLTG
160 170 180 190 200
TSKESYILHM IHLSEELGYR CVVFNNRGVA GENLLTPRTY CCANTEDLET
210 220 230 240 250
VIHHVHSLYP SAPFLAAGVS MGGMLLLNYL GKIGSKTPLM AAATFSVGWN
260 270 280 290 300
TFACSESLEK PLNWLLFNYY LTTCLQSSVN KHRHMFVKQV DMDHVMKAKS
310 320 330 340 350
IREFDKRFTS VMFGYQTIDD YYTDASPSPR LKSVGIPVLC LNSVDDVFSP
360 370 380 390 400
SHAIPIETAK QNPNVALVLT SYGGHIGFLE GIWPRQSTYM DRVFKQFVQA

MVEHGHELS
Length:409
Mass (Da):46,009
Last modified:March 20, 2007 - v2
Checksum:i9A3920EB7528273B
GO
Isoform 2 (identifier: Q8WU67-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     56-136: PQLVTGGESF...NDNSTCYMDA → RTTRRSGAGR...EQARSHISFI
     137-409: Missing.

Note: No experimental confirmation available.

Show »
Length:136
Mass (Da):15,525
Checksum:i61776799A97CA0A2
GO

Sequence cautioni

The sequence AAC19155.1 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti3 – 31R → C.1 Publication
Corresponds to variant rs17851878 [ dbSNP | Ensembl ].
VAR_031089

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei56 – 13681PQLVT…CYMDA → RTTRRSGAGRVEDRPCLDLS SLRSPRCSTGMNLLKLQMED RFHWTGLIMITVRVIWMPAP DLLSYCCLASREQARSHISF I in isoform 2. 1 PublicationVSP_056137Add
BLAST
Alternative sequencei137 – 409273Missing in isoform 2. 1 PublicationVSP_056138Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007152 mRNA. Translation: AAC19155.1. Different initiation.
AK298728 mRNA. Translation: BAH12858.1.
EF444943 Genomic DNA. Translation: ACA05928.1.
AC106037 Genomic DNA. No translation available.
CH471088 Genomic DNA. Translation: EAX01131.1.
BC021196 mRNA. Translation: AAH21196.1.
CCDSiCCDS32802.1. [Q8WU67-1]
RefSeqiNP_612213.2. NM_138340.4. [Q8WU67-1]
UniGeneiHs.397978.

Genome annotation databases

EnsembliENST00000289119; ENSP00000289119; ENSG00000158201. [Q8WU67-1]
ENST00000577891; ENSP00000463365; ENSG00000158201. [Q8WU67-2]
GeneIDi171586.
KEGGihsa:171586.
UCSCiuc002ktl.2. human. [Q8WU67-1]

Polymorphism and mutation databases

BioMutaiABHD3.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007152 mRNA. Translation: AAC19155.1. Different initiation.
AK298728 mRNA. Translation: BAH12858.1.
EF444943 Genomic DNA. Translation: ACA05928.1.
AC106037 Genomic DNA. No translation available.
CH471088 Genomic DNA. Translation: EAX01131.1.
BC021196 mRNA. Translation: AAH21196.1.
CCDSiCCDS32802.1. [Q8WU67-1]
RefSeqiNP_612213.2. NM_138340.4. [Q8WU67-1]
UniGeneiHs.397978.

3D structure databases

ProteinModelPortaliQ8WU67.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128149. 5 interactions.
IntActiQ8WU67. 1 interaction.
STRINGi9606.ENSP00000289119.

PTM databases

PhosphoSiteiQ8WU67.

Polymorphism and mutation databases

BioMutaiABHD3.
DMDMi134035377.

Proteomic databases

MaxQBiQ8WU67.
PaxDbiQ8WU67.
PRIDEiQ8WU67.

Protocols and materials databases

DNASUi171586.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000289119; ENSP00000289119; ENSG00000158201. [Q8WU67-1]
ENST00000577891; ENSP00000463365; ENSG00000158201. [Q8WU67-2]
GeneIDi171586.
KEGGihsa:171586.
UCSCiuc002ktl.2. human. [Q8WU67-1]

Organism-specific databases

CTDi171586.
GeneCardsiGC18M019230.
HGNCiHGNC:18718. ABHD3.
HPAiHPA010729.
HPA052862.
MIMi612197. gene.
neXtProtiNX_Q8WU67.
PharmGKBiPA38659.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0429.
GeneTreeiENSGT00530000062981.
HOGENOMiHOG000230596.
HOVERGENiHBG053994.
InParanoidiQ8WU67.
KOiK13696.
OMAiFITSKPQ.
OrthoDBiEOG70PBXF.
PhylomeDBiQ8WU67.
TreeFamiTF313195.

Miscellaneous databases

ChiTaRSiABHD3. human.
GenomeRNAii171586.
NextBioi35479691.
PROiQ8WU67.
SOURCEiSearch...

Gene expression databases

BgeeiQ8WU67.
CleanExiHS_ABHD3.
ExpressionAtlasiQ8WU67. baseline and differential.
GenevestigatoriQ8WU67.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR012020. AB-Hydro_YheT.
IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000952. UPF0017_hydro-like_CS.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF005211. Ab_hydro_YheT. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01133. UPF0017. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Yu W., Sarginson J., Gibbs R.A.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. NHLBI resequencing and genotyping service (RS&G)
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT CYS-3.
    Tissue: Lung.
  7. "Metabolomics annotates ABHD3 as a physiologic regulator of medium-chain phospholipids."
    Long J.Z., Cisar J.S., Milliken D., Niessen S., Wang C., Trauger S.A., Siuzdak G., Cravatt B.F.
    Nat. Chem. Biol. 7:763-765(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF SER-220.

Entry informationi

Entry nameiABHD3_HUMAN
AccessioniPrimary (citable) accession number: Q8WU67
Secondary accession number(s): B0YIV0, B7Z5C2, O43411
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: March 20, 2007
Last modified: April 29, 2015
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.