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Protein

Marginal zone B- and B1-cell-specific protein

Gene

MZB1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity (By similarity). Isoform 2 may be involved in regulation of apoptosis. Helps to diversify peripheral B-cell functions by regulating Ca2+ stores, antibody secretion and integrin activation.By similarity
Acts as a hormone-regulated adipokine/proinflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Marginal zone B- and B1-cell-specific protein
Alternative name(s):
Mesenteric estrogen-dependent adipose 7
Short name:
MEDA-7
Plasma cell-induced resident endoplasmic reticulum protein
Short name:
Plasma cell-induced resident ER protein
Short name:
pERp1
Proapoptotic caspase adapter protein
Gene namesi
Name:MZB1
Synonyms:MEDA7, PACAP
ORF Names:HSPC190
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:30125. MZB1.

Subcellular locationi

Isoform 1 :
Isoform 2 :
  • Cytoplasm

  • Note: Diffuse granular localization in the cytoplasm surrounding the nucleus.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • endoplasmic reticulum chaperone complex Source: UniProtKB
  • endoplasmic reticulum lumen Source: UniProtKB
  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi50 – 501C → S: Does not affect activity. 1 Publication
Mutagenesisi53 – 531C → S: No significant activity. 1 Publication
Mutagenesisi95 – 951C → S: No significant activity. 1 Publication
Mutagenesisi143 – 1431C → S: No significant activity. 1 Publication
Mutagenesisi171 – 1711C → S: No significant activity. 1 Publication
Mutagenesisi178 – 1781C → S: Does not affect activity. 1 Publication

Polymorphism and mutation databases

BioMutaiMZB1.
DMDMi74730663.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 189167Marginal zone B- and B1-cell-specific proteinPRO_0000318740Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 178By similarity
Disulfide bondi53 ↔ 171By similarity
Disulfide bondi95 ↔ 143By similarity

Post-translational modificationi

Forms an interchain disulfide bond with IgM monomers.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ8WU39.
PaxDbiQ8WU39.
PRIDEiQ8WU39.

PTM databases

PhosphoSiteiQ8WU39.

Expressioni

Tissue specificityi

Widely expressed with highest levels in adult brain, small intestine and lymphoid tissues such as thymus and spleen. Expression is frequently lower in intestinal-type gastric cancer. In obese patients, more abundant in omental than in subcutaneous fat.3 Publications

Inductioni

Down-regulated in primary B-cells early after ligand-stimulated activation. Up-regulated in bacterial lipopolysaccharides (LPS)-stimulated peritoneal macrophages.2 Publications

Gene expression databases

BgeeiQ8WU39.
ExpressionAtlasiQ8WU39. baseline and differential.
GenevisibleiQ8WU39. HS.

Organism-specific databases

HPAiHPA043745.

Interactioni

Subunit structurei

Part of the ER chaperone complex, a multi-protein complex in the endoplasmic reticulum containing a large number of molecular chaperones which associates with unassembled incompletely folded immunoglobulin heavy chains (By similarity). Isoform 2 interacts with CASP2 and CASP9. Interacts with HSP90B1 and PDIA3 in a calcium-dependent manner (By similarity).By similarity

Protein-protein interaction databases

BioGridi119399. 2 interactions.
IntActiQ8WU39. 1 interaction.
MINTiMINT-197959.
STRINGi9606.ENSP00000303920.

Structurei

3D structure databases

ProteinModelPortaliQ8WU39.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi186 – 1894Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the MZB1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG74024.
GeneTreeiENSGT00390000002716.
HOGENOMiHOG000068171.
HOVERGENiHBG108230.
InParanoidiQ8WU39.
OMAiEKYSAHM.
OrthoDBiEOG7D2FFZ.
PhylomeDBiQ8WU39.

Family and domain databases

InterProiIPR021852. DUF3456.
[Graphical view]
PfamiPF11938. DUF3456. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WU39-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLSLPLLLL LLGAWAIPGG LGDRAPLTAT APQLDDEEMY SAHMPAHLRC
60 70 80 90 100
DACRAVAYQM WQNLAKAETK LHTSNSGGRR ELSELVYTDV LDRSCSRNWQ
110 120 130 140 150
DYGVREVDQV KRLTGPGLSE GPEPSISVMV TGGPWPTRLS RTCLHYLGEF
160 170 180
GEDQIYEAHQ QGRGALEALL CGGPQGACSE KVSATREEL
Note: Major.
Length:189
Mass (Da):20,694
Last modified:March 1, 2002 - v1
Checksum:iC71AED212D3393D3
GO
Isoform 2 (identifier: Q8WU39-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-189: MWQNLAKAET...EKVSATREEL → SLLVPDVAKS...PSETSHRPRT

Show »
Length:123
Mass (Da):13,066
Checksum:iC21A354CE08A95CB
GO
Isoform 3 (identifier: Q8WU39-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.
     93-101: RSCSRNWQD → MPAELWLTS

Show »
Length:97
Mass (Da):10,488
Checksum:i8BA52E533824C02C
GO
Isoform 4 (identifier: Q8WU39-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-75: MWQNLAKAETKLHTSN → RLCSYPPHLPLTEQQS
     76-189: Missing.

Show »
Length:75
Mass (Da):8,227
Checksum:iC43C7E99CF029FF8
GO
Isoform 5 (identifier: Q8WU39-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     60-62: MWQ → AHP
     63-189: Missing.

Show »
Length:62
Mass (Da):6,681
Checksum:i7E1F097F7B9F33F4
GO

Sequence cautioni

The sequence AAF36110.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9292Missing in isoform 3. 1 PublicationVSP_031280Add
BLAST
Alternative sequencei60 – 189130MWQNL…TREEL → SLLVPDVAKSGKGRDQTSYL KLWGAAGAERVGLHGCPGPE LLPELAGLRSSRSGPSETSH RPRT in isoform 2. 1 PublicationVSP_031281Add
BLAST
Alternative sequencei60 – 7516MWQNL…LHTSN → RLCSYPPHLPLTEQQS in isoform 4. 1 PublicationVSP_038333Add
BLAST
Alternative sequencei60 – 623MWQ → AHP in isoform 5. 1 PublicationVSP_039073
Alternative sequencei63 – 189127Missing in isoform 5. 1 PublicationVSP_039074Add
BLAST
Alternative sequencei76 – 189114Missing in isoform 4. 1 PublicationVSP_038334Add
BLAST
Alternative sequencei93 – 1019RSCSRNWQD → MPAELWLTS in isoform 3. 1 PublicationVSP_038332

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF338109 mRNA. Translation: AAK84085.1.
AF151024 mRNA. Translation: AAF36110.1. Sequence problems.
BP366737 mRNA. No translation available.
GQ477350 mRNA. Translation: ACY74343.1.
AK292706 mRNA. Translation: BAF85395.1.
AL698690 mRNA. No translation available.
AC135457 Genomic DNA. No translation available.
BC009931 mRNA. Translation: AAH09931.2.
BC021275 mRNA. Translation: AAH21275.1.
CCDSiCCDS47273.1. [Q8WU39-1]
RefSeqiNP_057543.2. NM_016459.3. [Q8WU39-1]
UniGeneiHs.409563.

Genome annotation databases

EnsembliENST00000302125; ENSP00000303920; ENSG00000170476. [Q8WU39-1]
ENST00000417694; ENSP00000415420; ENSG00000170476. [Q8WU39-4]
ENST00000503481; ENSP00000423205; ENSG00000170476. [Q8WU39-2]
GeneIDi51237.
KEGGihsa:51237.
UCSCiuc003lei.3. human. [Q8WU39-1]
uc010jfd.3. human. [Q8WU39-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF338109 mRNA. Translation: AAK84085.1.
AF151024 mRNA. Translation: AAF36110.1. Sequence problems.
BP366737 mRNA. No translation available.
GQ477350 mRNA. Translation: ACY74343.1.
AK292706 mRNA. Translation: BAF85395.1.
AL698690 mRNA. No translation available.
AC135457 Genomic DNA. No translation available.
BC009931 mRNA. Translation: AAH09931.2.
BC021275 mRNA. Translation: AAH21275.1.
CCDSiCCDS47273.1. [Q8WU39-1]
RefSeqiNP_057543.2. NM_016459.3. [Q8WU39-1]
UniGeneiHs.409563.

3D structure databases

ProteinModelPortaliQ8WU39.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119399. 2 interactions.
IntActiQ8WU39. 1 interaction.
MINTiMINT-197959.
STRINGi9606.ENSP00000303920.

PTM databases

PhosphoSiteiQ8WU39.

Polymorphism and mutation databases

BioMutaiMZB1.
DMDMi74730663.

Proteomic databases

MaxQBiQ8WU39.
PaxDbiQ8WU39.
PRIDEiQ8WU39.

Protocols and materials databases

DNASUi51237.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000302125; ENSP00000303920; ENSG00000170476. [Q8WU39-1]
ENST00000417694; ENSP00000415420; ENSG00000170476. [Q8WU39-4]
ENST00000503481; ENSP00000423205; ENSG00000170476. [Q8WU39-2]
GeneIDi51237.
KEGGihsa:51237.
UCSCiuc003lei.3. human. [Q8WU39-1]
uc010jfd.3. human. [Q8WU39-3]

Organism-specific databases

CTDi51237.
GeneCardsiGC05M138723.
HGNCiHGNC:30125. MZB1.
HPAiHPA043745.
MIMi609447. gene.
neXtProtiNX_Q8WU39.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG74024.
GeneTreeiENSGT00390000002716.
HOGENOMiHOG000068171.
HOVERGENiHBG108230.
InParanoidiQ8WU39.
OMAiEKYSAHM.
OrthoDBiEOG7D2FFZ.
PhylomeDBiQ8WU39.

Miscellaneous databases

ChiTaRSiMZB1. human.
GenomeRNAii51237.
NextBioi54355.
PROiQ8WU39.
SOURCEiSearch...

Gene expression databases

BgeeiQ8WU39.
ExpressionAtlasiQ8WU39. baseline and differential.
GenevisibleiQ8WU39. HS.

Family and domain databases

InterProiIPR021852. DUF3456.
[Graphical view]
PfamiPF11938. DUF3456. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a novel proapoptotic caspase-2- and caspase-9-binding protein."
    Bonfoco E., Li E., Kolbinger F., Cooper N.R.
    J. Biol. Chem. 276:29242-29250(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, INTERACTION WITH CASP2 AND CASP9.
  2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
    Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X.
    , Gu J., Chen S.-J., Chen Z.
    Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
    Tissue: Umbilical cord blood.
  3. "pERp1 is significantly up-regulated during plasma cell differentiation and contributes to the oxidative folding of immunoglobulin."
    Shimizu Y., Meunier L., Hendershot L.M.
    Proc. Natl. Acad. Sci. U.S.A. 106:17013-17018(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF CYS-50; CYS-53; CYS-95; CYS-143; CYS-171 AND CYS-178.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Thymus.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
  6. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Tissue: B-cell and Lymph.
  8. "MGC29506 gene, frequently down-regulated in intestinal-type gastric cancer, encodes secreted-type protein with conserved cysteine residues."
    Katoh M., Katoh M.
    Int. J. Oncol. 23:235-241(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Novel hormone-regulated genes in visceral adipose tissue: cloning and identification of proinflammatory cytokine-like mouse and human MEDA-7: implications for obesity, insulin resistance and the metabolic syndrome."
    Zhang H., Chen X., Sairam M.R.
    Diabetologia 54:2368-2380(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INDUCTION, FUNCTION.

Entry informationi

Entry nameiMZB1_HUMAN
AccessioniPrimary (citable) accession number: Q8WU39
Secondary accession number(s): D2IYS0
, Q7Z6N2, Q96RL5, Q9P0T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2002
Last modified: June 24, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.