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Q8WU39 (MZB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Marginal zone B- and B1-cell-specific protein
Alternative name(s):
Mesenteric estrogen-dependent adipose 7
Short name=MEDA-7
Plasma cell-induced resident endoplasmic reticulum protein
Short name=Plasma cell-induced resident ER protein
Short name=pERp1
Proapoptotic caspase adapter protein
Gene names
Name:MZB1
Synonyms:MEDA7, PACAP
ORF Names:HSPC190
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. May exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity By similarity. Isoform 2 may be involved in regulation of apoptosis. Helps to diversify peripheral B-cell functions by regulating Ca2+ stores, antibody secretion and integrin activation. Ref.1 Ref.10

Acts as a hormone-regulated adipokine/proinflammatory cytokine that is implicated in causing chronic inflammation, affecting cellular expansion and blunting insulin response in adipocytes. May have a role in the onset of insulin resistance. Ref.1 Ref.10

Subunit structure

Part of the ER chaperone complex, a multi-protein complex in the endoplasmic reticulum containing a large number of molecular chaperones which associates with unassembled incompletely folded immunoglobulin heavy chains By similarity. Isoform 2 interacts with CASP2 and CASP9. Interacts with HSP90B1 and PDIA3 in a calcium-dependent manner By similarity. Ref.1

Subcellular location

Isoform 1: Endoplasmic reticulum lumen By similarity. Secreted Ref.1 Ref.3 Ref.10.

Isoform 2: Cytoplasm. Note: Diffuse granular localization in the cytoplasm surrounding the nucleus. Ref.1 Ref.3 Ref.10

Tissue specificity

Widely expressed with highest levels in adult brain, small intestine and lymphoid tissues such as thymus and spleen. Expression is frequently lower in intestinal-type gastric cancer. In obese patients, more abundant in omental than in subcutaneous fat. Ref.1 Ref.3 Ref.8

Induction

Down-regulated in primary B-cells early after ligand-stimulated activation. Up-regulated in bacterial lipopolysaccharides (LPS)-stimulated peritoneal macrophages. Ref.1 Ref.10

Post-translational modification

Forms an interchain disulfide bond with IgM monomers By similarity.

Sequence similarities

Belongs to the MZB1 family.

Sequence caution

The sequence AAF36110.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WU39-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Major.
Isoform 2 (identifier: Q8WU39-2)

The sequence of this isoform differs from the canonical sequence as follows:
     60-189: MWQNLAKAET...EKVSATREEL → SLLVPDVAKS...PSETSHRPRT
Isoform 3 (identifier: Q8WU39-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.
     93-101: RSCSRNWQD → MPAELWLTS
Isoform 4 (identifier: Q8WU39-4)

The sequence of this isoform differs from the canonical sequence as follows:
     60-75: MWQNLAKAETKLHTSN → RLCSYPPHLPLTEQQS
     76-189: Missing.
Isoform 5 (identifier: Q8WU39-5)

The sequence of this isoform differs from the canonical sequence as follows:
     60-62: MWQ → AHP
     63-189: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 189167Marginal zone B- and B1-cell-specific protein
PRO_0000318740

Regions

Motif186 – 1894Prevents secretion from ER Potential

Amino acid modifications

Disulfide bond50 ↔ 178 By similarity
Disulfide bond53 ↔ 171 By similarity
Disulfide bond95 ↔ 143 By similarity

Natural variations

Alternative sequence1 – 9292Missing in isoform 3.
VSP_031280
Alternative sequence60 – 189130MWQNL…TREEL → SLLVPDVAKSGKGRDQTSYL KLWGAAGAERVGLHGCPGPE LLPELAGLRSSRSGPSETSH RPRT in isoform 2.
VSP_031281
Alternative sequence60 – 7516MWQNL…LHTSN → RLCSYPPHLPLTEQQS in isoform 4.
VSP_038333
Alternative sequence60 – 623MWQ → AHP in isoform 5.
VSP_039073
Alternative sequence63 – 189127Missing in isoform 5.
VSP_039074
Alternative sequence76 – 189114Missing in isoform 4.
VSP_038334
Alternative sequence93 – 1019RSCSRNWQD → MPAELWLTS in isoform 3.
VSP_038332

Experimental info

Mutagenesis501C → S: Does not affect activity. Ref.3
Mutagenesis531C → S: No significant activity. Ref.3
Mutagenesis951C → S: No significant activity. Ref.3
Mutagenesis1431C → S: No significant activity. Ref.3
Mutagenesis1711C → S: No significant activity. Ref.3
Mutagenesis1781C → S: Does not affect activity. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C71AED212D3393D3

FASTA18920,694
        10         20         30         40         50         60 
MRLSLPLLLL LLGAWAIPGG LGDRAPLTAT APQLDDEEMY SAHMPAHLRC DACRAVAYQM 

        70         80         90        100        110        120 
WQNLAKAETK LHTSNSGGRR ELSELVYTDV LDRSCSRNWQ DYGVREVDQV KRLTGPGLSE 

       130        140        150        160        170        180 
GPEPSISVMV TGGPWPTRLS RTCLHYLGEF GEDQIYEAHQ QGRGALEALL CGGPQGACSE 


KVSATREEL 

« Hide

Isoform 2 [UniParc].

Checksum: C21A354CE08A95CB
Show »

FASTA12313,066
Isoform 3 [UniParc].

Checksum: 8BA52E533824C02C
Show »

FASTA9710,488
Isoform 4 [UniParc].

Checksum: C43C7E99CF029FF8
Show »

FASTA758,227
Isoform 5 [UniParc].

Checksum: 7E1F097F7B9F33F4
Show »

FASTA626,681

References

« Hide 'large scale' references
[1]"Characterization of a novel proapoptotic caspase-2- and caspase-9-binding protein."
Bonfoco E., Li E., Kolbinger F., Cooper N.R.
J. Biol. Chem. 276:29242-29250(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, INTERACTION WITH CASP2 AND CASP9.
[2]"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X. expand/collapse author list , Gu J., Chen S.-J., Chen Z.
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Umbilical cord blood.
[3]"pERp1 is significantly up-regulated during plasma cell differentiation and contributes to the oxidative folding of immunoglobulin."
Shimizu Y., Meunier L., Hendershot L.M.
Proc. Natl. Acad. Sci. U.S.A. 106:17013-17018(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MUTAGENESIS OF CYS-50; CYS-53; CYS-95; CYS-143; CYS-171 AND CYS-178.
[4]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Thymus.
[5]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
[6]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Tissue: B-cell and Lymph.
[8]"MGC29506 gene, frequently down-regulated in intestinal-type gastric cancer, encodes secreted-type protein with conserved cysteine residues."
Katoh M., Katoh M.
Int. J. Oncol. 23:235-241(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Novel hormone-regulated genes in visceral adipose tissue: cloning and identification of proinflammatory cytokine-like mouse and human MEDA-7: implications for obesity, insulin resistance and the metabolic syndrome."
Zhang H., Chen X., Sairam M.R.
Diabetologia 54:2368-2380(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INDUCTION, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF338109 mRNA. Translation: AAK84085.1.
AF151024 mRNA. Translation: AAF36110.1. Sequence problems.
BP366737 mRNA. No translation available.
GQ477350 mRNA. Translation: ACY74343.1.
AK292706 mRNA. Translation: BAF85395.1.
AL698690 mRNA. No translation available.
AC135457 Genomic DNA. No translation available.
BC009931 mRNA. Translation: AAH09931.2.
BC021275 mRNA. Translation: AAH21275.1.
CCDSCCDS47273.1. [Q8WU39-1]
RefSeqNP_057543.2. NM_016459.3. [Q8WU39-1]
UniGeneHs.409563.

3D structure databases

ProteinModelPortalQ8WU39.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119399. 2 interactions.
IntActQ8WU39. 1 interaction.
MINTMINT-197959.

PTM databases

PhosphoSiteQ8WU39.

Polymorphism databases

DMDM74730663.

Proteomic databases

MaxQBQ8WU39.
PaxDbQ8WU39.
PRIDEQ8WU39.

Protocols and materials databases

DNASU51237.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000302125; ENSP00000303920; ENSG00000170476. [Q8WU39-1]
ENST00000412103; ENSP00000414565; ENSG00000170476. [Q8WU39-3]
ENST00000417694; ENSP00000415420; ENSG00000170476. [Q8WU39-4]
ENST00000457570; ENSP00000406529; ENSG00000170476. [Q8WU39-4]
ENST00000503481; ENSP00000423205; ENSG00000170476. [Q8WU39-2]
GeneID51237.
KEGGhsa:51237.
UCSCuc003lei.3. human. [Q8WU39-1]
uc010jfd.3. human. [Q8WU39-3]

Organism-specific databases

CTD51237.
GeneCardsGC05M138723.
HGNCHGNC:30125. MZB1.
HPAHPA043745.
MIM609447. gene.
neXtProtNX_Q8WU39.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG74024.
HOGENOMHOG000068171.
HOVERGENHBG108230.
InParanoidQ8WU39.
OMAYQMWQHL.
OrthoDBEOG7D2FFZ.
PhylomeDBQ8WU39.

Gene expression databases

ArrayExpressQ8WU39.
BgeeQ8WU39.
GenevestigatorQ8WU39.

Family and domain databases

InterProIPR021852. DUF3456.
[Graphical view]
PfamPF11938. DUF3456. 1 hit.
[Graphical view]
PROSITEPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMZB1. human.
GenomeRNAi51237.
NextBio54355.
PROQ8WU39.
SOURCESearch...

Entry information

Entry nameMZB1_HUMAN
AccessionPrimary (citable) accession number: Q8WU39
Secondary accession number(s): D2IYS0 expand/collapse secondary AC list , Q7Z6N2, Q96RL5, Q9P0T3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM