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Protein

Serine/threonine-protein kinase 32A

Gene

STK32A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521ATPPROSITE-ProRule annotationBy similarity
Active sitei146 – 1461Proton acceptorPROSITE-ProRule annotationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi29 – 379ATPPROSITE-ProRule annotationBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. regulation of mitochondrion degradation Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiQ8WU08.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 32A (EC:2.7.11.1)
Alternative name(s):
Yet another novel kinase 1
Gene namesi
Name:STK32A
Synonyms:YANK1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:28317. STK32A.

Subcellular locationi

  1. Cell membrane; Lipid-anchor 1 Publication

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21G → A: Localizes to cytoplasm. 1 Publication

Organism-specific databases

PharmGKBiPA134932545.

Polymorphism and mutation databases

BioMutaiSTK32A.
DMDMi93140693.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 396395Serine/threonine-protein kinase 32APRO_0000232411Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiQ8WU08.
PRIDEiQ8WU08.

PTM databases

PhosphoSiteiQ8WU08.

Expressioni

Gene expression databases

BgeeiQ8WU08.
CleanExiHS_STK32A.
ExpressionAtlasiQ8WU08. baseline and differential.
GenevestigatoriQ8WU08.

Organism-specific databases

HPAiHPA040236.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000381030.

Structurei

Secondary structure

1
396
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 223Combined sources
Beta strandi23 – 308Combined sources
Beta strandi37 – 426Combined sources
Turni43 – 453Combined sources
Beta strandi48 – 558Combined sources
Helixi56 – 616Combined sources
Helixi65 – 7612Combined sources
Beta strandi86 – 916Combined sources
Beta strandi93 – 1008Combined sources
Helixi108 – 1136Combined sources
Helixi120 – 13920Combined sources
Beta strandi151 – 1544Combined sources
Beta strandi160 – 1623Combined sources
Helixi184 – 1863Combined sources
Helixi189 – 1913Combined sources
Helixi204 – 21815Combined sources
Helixi231 – 24010Combined sources
Helixi251 – 26010Combined sources
Helixi265 – 2673Combined sources
Helixi272 – 2765Combined sources
Helixi279 – 2813Combined sources
Helixi286 – 2905Combined sources
Helixi343 – 35816Combined sources
Helixi364 – 3696Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FR4X-ray2.29A/B/C/D/E/F9-390[»]
ProteinModelPortaliQ8WU08.
SMRiQ8WU08. Positions 14-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 281259Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00780000121918.
HOGENOMiHOG000021314.
HOVERGENiHBG106458.
InParanoidiQ8WU08.
KOiK08793.
OrthoDBiEOG7RZ5Q0.
PhylomeDBiQ8WU08.
TreeFamiTF313395.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1Curated (identifier: Q8WU08-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGANTSRKPP VFDENEDVNF DHFEILRAIG KGSFGKVCIV QKNDTKKMYA
60 70 80 90 100
MKYMNKQKCV ERNEVRNVFK ELQIMQGLEH PFLVNLWYSF QDEEDMFMVV
110 120 130 140 150
DLLLGGDLRY HLQQNVHFKE ETVKLFICEL VMALDYLQNQ RIIHRDMKPD
160 170 180 190 200
NILLDEHGHV HITDFNIAAM LPRETQITTM AGTKPYMAPE MFSSRKGAGY
210 220 230 240 250
SFAVDWWSLG VTAYELLRGR RPYHIRSSTS SKEIVHTFET TVVTYPSAWS
260 270 280 290 300
QEMVSLLKKL LEPNPDQRFS QLSDVQNFPY MNDINWDAVF QKRLIPGFIP
310 320 330 340 350
NKGRLNCDPT FELEEMILES KPLHKKKKRL AKKEKDMRKC DSSQTCLLQE
360 370 380 390
HLDSVQKEFI IFNREKVNRD FNKRQPNLAL EQTKDPQGED GQNNNL
Length:396
Mass (Da):46,369
Last modified:April 18, 2006 - v2
Checksum:i5ED718F0D3A7A48C
GO
Isoform 21 Publication (identifier: Q8WU08-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     158-166: GHVHITDFN → DTWLSYKSH
     167-396: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:166
Mass (Da):19,792
Checksum:iCDCF94E54C793BA4
GO
Isoform 3Curated (identifier: Q8WU08-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     345-358: TCLLQEHLDSVQKE → VSRSPPNSGSWVSP
     359-396: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:358
Mass (Da):41,699
Checksum:i65A66B1C7627265A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti58 – 581K → M.1 Publication
Corresponds to variant rs35852718 [ dbSNP | Ensembl ].
VAR_041163
Natural varianti89 – 891S → F in a metastatic melanoma sample; somatic mutation. 1 Publication
VAR_041164
Natural varianti316 – 3161M → I in a lung neuroendocrine carcinoma sample; somatic mutation. 1 Publication
VAR_041165

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei158 – 1669GHVHITDFN → DTWLSYKSH in isoform 2. 1 PublicationVSP_051992
Alternative sequencei167 – 396230Missing in isoform 2. 1 PublicationVSP_051993Add
BLAST
Alternative sequencei345 – 35814TCLLQ…SVQKE → VSRSPPNSGSWVSP in isoform 3. 1 PublicationVSP_051994Add
BLAST
Alternative sequencei359 – 39638Missing in isoform 3. 1 PublicationVSP_051995Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK094580 mRNA. Translation: BAG52892.1.
AC008740 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61827.1.
CCDSiCCDS47299.1. [Q8WU08-1]
RefSeqiNP_001106195.1. NM_001112724.1. [Q8WU08-1]
NP_659438.1. NM_145001.3. [Q8WU08-2]
UniGeneiHs.585069.

Genome annotation databases

EnsembliENST00000397936; ENSP00000381030; ENSG00000169302. [Q8WU08-1]
GeneIDi202374.
KEGGihsa:202374.
UCSCiuc003lol.4. human. [Q8WU08-2]
uc003lom.2. human. [Q8WU08-3]
uc010jgn.1. human. [Q8WU08-1]

Polymorphism and mutation databases

BioMutaiSTK32A.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK094580 mRNA. Translation: BAG52892.1.
AC008740 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61827.1.
CCDSiCCDS47299.1. [Q8WU08-1]
RefSeqiNP_001106195.1. NM_001112724.1. [Q8WU08-1]
NP_659438.1. NM_145001.3. [Q8WU08-2]
UniGeneiHs.585069.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4FR4X-ray2.29A/B/C/D/E/F9-390[»]
ProteinModelPortaliQ8WU08.
SMRiQ8WU08. Positions 14-373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000381030.

Chemistry

BindingDBiQ8WU08.
ChEMBLiCHEMBL6150.
GuidetoPHARMACOLOGYi1537.

PTM databases

PhosphoSiteiQ8WU08.

Polymorphism and mutation databases

BioMutaiSTK32A.
DMDMi93140693.

Proteomic databases

PaxDbiQ8WU08.
PRIDEiQ8WU08.

Protocols and materials databases

DNASUi202374.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000397936; ENSP00000381030; ENSG00000169302. [Q8WU08-1]
GeneIDi202374.
KEGGihsa:202374.
UCSCiuc003lol.4. human. [Q8WU08-2]
uc003lom.2. human. [Q8WU08-3]
uc010jgn.1. human. [Q8WU08-1]

Organism-specific databases

CTDi202374.
GeneCardsiGC05P146594.
H-InvDBHIX0005283.
HGNCiHGNC:28317. STK32A.
HPAiHPA040236.
neXtProtiNX_Q8WU08.
PharmGKBiPA134932545.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00780000121918.
HOGENOMiHOG000021314.
HOVERGENiHBG106458.
InParanoidiQ8WU08.
KOiK08793.
OrthoDBiEOG7RZ5Q0.
PhylomeDBiQ8WU08.
TreeFamiTF313395.

Enzyme and pathway databases

SignaLinkiQ8WU08.

Miscellaneous databases

GenomeRNAii202374.
NextBioi90285.
PROiQ8WU08.

Gene expression databases

BgeeiQ8WU08.
CleanExiHS_STK32A.
ExpressionAtlasiQ8WU08. baseline and differential.
GenevestigatoriQ8WU08.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NOMENCLATURE.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Amygdala1 Publication.
  3. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Urinary bladder.
  5. "Cell-free identification of novel N-myristoylated proteins from complementary DNA resources using bioorthogonal myristic acid analogues."
    Takamitsu E., Fukunaga K., Iio Y., Moriya K., Utsumi T.
    Anal. Biochem. 464:83-93(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: MIRISTOYLATION AT GLY-2, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-2.
  6. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-58; PHE-89 AND ILE-316.

Entry informationi

Entry nameiST32A_HUMAN
AccessioniPrimary (citable) accession number: Q8WU08
Secondary accession number(s): B3KSY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: April 18, 2006
Last modified: April 29, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.