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Q8WU08 (ST32A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase 32A

EC=2.7.11.1
Alternative name(s):
Yet another novel kinase 1
Gene names
Name:STK32A
Synonyms:YANK1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB Q60592

Cofactor

Magnesium By similarity. UniProtKB Q60592

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8WU08-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.4 (identifier: Q8WU08-2)

The sequence of this isoform differs from the canonical sequence as follows:
     158-166: GHVHITDFN → DTWLSYKSH
     167-396: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8WU08-3)

The sequence of this isoform differs from the canonical sequence as follows:
     345-358: TCLLQEHLDSVQKE → VSRSPPNSGSWVSP
     359-396: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396Serine/threonine-protein kinase 32A
PRO_0000232411

Regions

Domain23 – 281259Protein kinase
Nucleotide binding29 – 379ATP By similarity UniProtKB Q60592

Sites

Active site1461Proton acceptor By similarity UniProtKB Q60592
Binding site521ATP By similarity UniProtKB Q60592

Natural variations

Alternative sequence158 – 1669GHVHITDFN → DTWLSYKSH in isoform 2.
VSP_051992
Alternative sequence167 – 396230Missing in isoform 2. Ref.4
VSP_051993
Alternative sequence345 – 35814TCLLQ…SVQKE → VSRSPPNSGSWVSP in isoform 3.
VSP_051994
Alternative sequence359 – 39638Missing in isoform 3.
VSP_051995
Natural variant581K → M. Ref.5
Corresponds to variant rs35852718 [ dbSNP | Ensembl ].
VAR_041163
Natural variant891S → F in a metastatic melanoma sample; somatic mutation. Ref.5
VAR_041164
Natural variant3161M → I in a lung neuroendocrine carcinoma sample; somatic mutation. Ref.5
VAR_041165

Secondary structure

.............................................. 396
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 18, 2006. Version 2.
Checksum: 5ED718F0D3A7A48C

FASTA39646,369
        10         20         30         40         50         60 
MGANTSRKPP VFDENEDVNF DHFEILRAIG KGSFGKVCIV QKNDTKKMYA MKYMNKQKCV 

        70         80         90        100        110        120 
ERNEVRNVFK ELQIMQGLEH PFLVNLWYSF QDEEDMFMVV DLLLGGDLRY HLQQNVHFKE 

       130        140        150        160        170        180 
ETVKLFICEL VMALDYLQNQ RIIHRDMKPD NILLDEHGHV HITDFNIAAM LPRETQITTM 

       190        200        210        220        230        240 
AGTKPYMAPE MFSSRKGAGY SFAVDWWSLG VTAYELLRGR RPYHIRSSTS SKEIVHTFET 

       250        260        270        280        290        300 
TVVTYPSAWS QEMVSLLKKL LEPNPDQRFS QLSDVQNFPY MNDINWDAVF QKRLIPGFIP 

       310        320        330        340        350        360 
NKGRLNCDPT FELEEMILES KPLHKKKKRL AKKEKDMRKC DSSQTCLLQE HLDSVQKEFI 

       370        380        390 
IFNREKVNRD FNKRQPNLAL EQTKDPQGED GQNNNL 

« Hide

Isoform 2 [UniParc].

Checksum: CDCF94E54C793BA4
Show »

FASTA16619,792
Isoform 3 [UniParc].

Checksum: 65A66B1C7627265A
Show »

FASTA35841,699

References

« Hide 'large scale' references
[1]"The protein kinase complement of the human genome."
Manning G., Whyte D.B., Martinez R., Hunter T., Sudarsanam S.
Science 298:1912-1934(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NOMENCLATURE.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Amygdala.
[3]"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. expand/collapse author list , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Urinary bladder.
[5]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-58; PHE-89 AND ILE-316.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK094580 mRNA. Translation: BAG52892.1.
AC008740 Genomic DNA. No translation available.
CH471062 Genomic DNA. Translation: EAW61827.1.
CCDSCCDS47299.1. [Q8WU08-1]
CCDS54931.1. [Q8WU08-2]
RefSeqNP_001106195.1. NM_001112724.1. [Q8WU08-1]
NP_659438.1. NM_145001.3. [Q8WU08-2]
UniGeneHs.585069.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4FR4X-ray2.29A/B/C/D/E/F9-390[»]
ProteinModelPortalQ8WU08.
SMRQ8WU08. Positions 14-373.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000381030.

Chemistry

BindingDBQ8WU08.
ChEMBLCHEMBL6150.
GuidetoPHARMACOLOGY1537.

PTM databases

PhosphoSiteQ8WU08.

Polymorphism databases

DMDM93140693.

Proteomic databases

PaxDbQ8WU08.
PRIDEQ8WU08.

Protocols and materials databases

DNASU202374.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000397936; ENSP00000381030; ENSG00000169302. [Q8WU08-1]
ENST00000541094; ENSP00000443156; ENSG00000169302. [Q8WU08-2]
GeneID202374.
KEGGhsa:202374.
UCSCuc003lol.4. human. [Q8WU08-2]
uc003lom.2. human. [Q8WU08-3]
uc010jgn.1. human. [Q8WU08-1]

Organism-specific databases

CTD202374.
GeneCardsGC05P146594.
H-InvDBHIX0005283.
HGNCHGNC:28317. STK32A.
HPAHPA040236.
neXtProtNX_Q8WU08.
PharmGKBPA134932545.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000021314.
HOVERGENHBG106458.
InParanoidQ8WU08.
KOK08793.
OrthoDBEOG7RZ5Q0.
PhylomeDBQ8WU08.
TreeFamTF313395.

Enzyme and pathway databases

SignaLinkQ8WU08.

Gene expression databases

ArrayExpressQ8WU08.
BgeeQ8WU08.
CleanExHS_STK32A.
GenevestigatorQ8WU08.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi202374.
NextBio90285.
PROQ8WU08.

Entry information

Entry nameST32A_HUMAN
AccessionPrimary (citable) accession number: Q8WU08
Secondary accession number(s): B3KSY0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: April 18, 2006
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM