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Protein

Anamorsin

Gene

Ciapin1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the scaffold complex NUBP1-NUBP2. Electrons are transferred to CIAPIN1 from NADPH via the FAD- and FMN-containing protein NDOR1. NDOR1-CIAPIN1 are also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit (By similarity). Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells (PubMed:14970183).UniRule annotation1 Publication

Miscellaneous

'Ana-mors-in' means 'anti-death molecule' in Latin.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotation
  • [4Fe-4S] clusterUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi235Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi244Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi247Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi249Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi271Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi274Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi282Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi285Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • hemopoiesis Source: MGI
  • iron-sulfur cluster assembly Source: GO_Central
  • negative regulation of apoptotic process Source: MGI

Keywordsi

Biological processApoptosis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
AnamorsinUniRule annotation
Alternative name(s):
Cytokine-induced apoptosis inhibitor 1UniRule annotation
Fe-S cluster assembly protein DRE2 homologUniRule annotation
Gene namesi
Name:Ciapin1UniRule annotation
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1922083 Ciapin1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death in late gestation due to defective definitive hematopoiesis in the fetal liver, possibly due to initiated apoptosis in erythroid cells during terminal maturation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000792891 – 309AnamorsinAdd BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei182PhosphoserineBy similarity1
Modified residuei183PhosphoserineBy similarity1
Modified residuei213PhosphoserineBy similarity1
Modified residuei269PhosphoserineCombined sources1
Modified residuei302PhosphoserineBy similarity1
Modified residuei304PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8WTY4
MaxQBiQ8WTY4
PaxDbiQ8WTY4
PeptideAtlasiQ8WTY4
PRIDEiQ8WTY4

PTM databases

iPTMnetiQ8WTY4
PhosphoSitePlusiQ8WTY4

Expressioni

Developmental stagei

Expressed from early embryogenesis.

Inductioni

By cytokines such as IL3 and THPO.1 Publication

Gene expression databases

BgeeiENSMUSG00000031781
CleanExiMM_CIAPIN1
ExpressionAtlasiQ8WTY4 baseline and differential
GenevisibleiQ8WTY4 MM

Interactioni

Subunit structurei

Monomer. Interacts with NDOR1. Interacts with CHCHD4.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
Glrx3Q9CQM94EBI-2943068,EBI-4319195

Protein-protein interaction databases

IntActiQ8WTY4, 4 interactors
MINTiQ8WTY4
STRINGi10090.ENSMUSP00000125451

Structurei

3D structure databases

ProteinModelPortaliQ8WTY4
SMRiQ8WTY4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 172N-terminal SAM-like domainUniRule annotationAdd BLAST167
Regioni173 – 222LinkerUniRule annotationAdd BLAST50
Regioni223 – 309Intrinsically disorderedUniRule annotationAdd BLAST87
Regioni235 – 249Fe-S binding site AUniRule annotationAdd BLAST15
Regioni271 – 285Fe-S binding site BUniRule annotationAdd BLAST15

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi271 – 274Cx2C motif 1UniRule annotation4
Motifi282 – 285Cx2C motif 2UniRule annotation4

Domaini

The twin Cx2C motifs are involved in the recognition by the mitochondrial CHCHD4/MIA40-GFER/ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.UniRule annotation
The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.UniRule annotation
The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.UniRule annotation

Sequence similaritiesi

Belongs to the anamorsin family.UniRule annotation

Phylogenomic databases

eggNOGiKOG4020 Eukaryota
COG5636 LUCA
GeneTreeiENSGT00390000011417
HOVERGENiHBG051104
InParanoidiQ8WTY4
OMAiQFVAVVW
OrthoDBiEOG091G0OTI
PhylomeDBiQ8WTY4
TreeFamiTF314449

Family and domain databases

HAMAPiMF_03115 Anamorsin, 1 hit
InterProiView protein in InterPro
IPR007785 Anamorsin
IPR013216 Methyltransf_11
IPR029063 SAM-dependent_MTases
PANTHERiPTHR13273 PTHR13273, 1 hit
PfamiView protein in Pfam
PF05093 CIAPIN1, 2 hits
PF08241 Methyltransf_11, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Q8WTY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEFGISPGQ LVAVFWDKSS PEEALKKLVA RLQELTGSEG QVFMENVTQL
60 70 80 90 100
LQSSHKESSF DVILSGVVPG STSLHSAEVL AEMARILRPG GCLFLKEPVE
110 120 130 140 150
TAEVNNDKMK TASKLCSALT LSGLVEIKEL QREALSPEEV QSVQEHLGYH
160 170 180 190 200
SDSLRSVRVT GKKPNFEVGS SSQLKLPNKK SSSVKPVVDP AAAKLWTLSA
210 220 230 240 250
NDMEDDSVDL IDSDELLDPE DLKRPDPASL KAPSCGEGKK RKACKNCTCG
260 270 280 290 300
LAEELEREQS KAQSSQPKSA CGNCYLGDAF RCANCPYLGM PAFKPGEQVL

LSNSNLQDA
Length:309
Mass (Da):33,429
Last modified:March 1, 2002 - v1
Checksum:i2F1FCB614767C915
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY523555 mRNA Translation: AAS09959.1
AK076116 mRNA Translation: BAC36196.1
AK079615 mRNA Translation: BAC37702.1
AK088757 mRNA Translation: BAC40550.1
AK136821 mRNA Translation: BAE23137.1
AK146282 mRNA Translation: BAE27040.1
AK163705 mRNA Translation: BAE37466.1
AK168252 mRNA Translation: BAE40202.1
BC016261 mRNA Translation: AAH16261.1
BC021864 mRNA Translation: AAH21864.1
BC021949 mRNA Translation: AAH21949.1
CCDSiCCDS22549.1
RefSeqiNP_598902.1, NM_134141.4
XP_006530623.1, XM_006530560.1
UniGeneiMm.27582
Mm.472546

Genome annotation databases

EnsembliENSMUST00000034233; ENSMUSP00000034233; ENSMUSG00000031781
ENSMUST00000162538; ENSMUSP00000125451; ENSMUSG00000031781
GeneIDi109006
KEGGimmu:109006
UCSCiuc009mxa.2 mouse

Similar proteinsi

Entry informationi

Entry nameiCPIN1_MOUSE
AccessioniPrimary (citable) accession number: Q8WTY4
Secondary accession number(s): Q3UJW5, Q8VC24, Q91W83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: March 1, 2002
Last modified: April 25, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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