Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

C-type lectin domain family 4 member C

Gene

CLEC4C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lectin-type cell surface receptor which may play a role in antigen capturing by dendritic cells (PubMed:11748283, PubMed:21880719, PubMed:25995448). Specifically recognizes non-sialylated galactose-terminated biantennary glycans containing the trisaccharide epitope Gal(beta1-3/4)GlcNAc(beta1-2)Man (PubMed:21880719, PubMed:25995448). Binds to serum IgG (PubMed:25995448). Efficiently targets ligand into antigen-processing and peptide-loading compartments for presentation to T-cells (PubMed:11748283). May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells (PubMed:11748283, PubMed:21880719). May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium (PubMed:11748283).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei139CarbohydrateCombined sources1 Publication1
Metal bindingi172CalciumCombined sources1 Publication1
Metal bindingi174CalciumCombined sources1 Publication1
Metal bindingi178CalciumCombined sources1 Publication1
Binding sitei178CarbohydrateCombined sources1 Publication1
Metal bindingi194CalciumCombined sources1 Publication1
Binding sitei194CarbohydrateCombined sources1 Publication1
Metal bindingi195CalciumCombined sources1 Publication1
Binding sitei202CarbohydrateCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAdaptive immunity, Immunity, Innate immunity
LigandCalcium, Lectin, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-5621480. Dectin-2 family.
R-HSA-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiI63.002.

Names & Taxonomyi

Protein namesi
Recommended name:
C-type lectin domain family 4 member C
Alternative name(s):
Blood dendritic cell antigen 2
Short name:
BDCA-2
C-type lectin superfamily member 7
Dendritic lectin
CD_antigen: CD303
Gene namesi
Name:CLEC4C
Synonyms:BDCA2, CLECSF11, CLECSF7, DLEC, HECL
ORF Names:UNQ9361/PRO34150
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000198178.10.
HGNCiHGNC:13258. CLEC4C.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 44Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini45 – 213ExtracellularSequence analysisAdd BLAST169

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi139S → A: Significantly impairs carbohydrate binding for the trisaccharide Gal(beta1-3/4)GlcNAc(beta1-2)Man. 1 Publication1
Mutagenesisi184N → A: Abolishes carbohydrate binding for the trisaccharide Gal(beta1-3/4)GlcNAc(beta1-2)Man. 1 Publication1
Mutagenesisi186R → A: Significantly impairs carbohydrate binding for the trisaccharide Gal(beta1-3/4)GlcNAc(beta1-2)Man. 1 Publication1
Mutagenesisi200V → A: Significantly impairs carbohydrate binding for the trisaccharide Gal(beta1-3/4)GlcNAc(beta1-2)Man. 1 Publication1
Mutagenesisi202Q → A: Significantly impairs carbohydrate binding for the trisaccharide Gal(beta1-3/4)GlcNAc(beta1-2)Man. 1 Publication1

Organism-specific databases

DisGeNETi170482.
OpenTargetsiENSG00000198178.
PharmGKBiPA26585.

Chemistry databases

ChEMBLiCHEMBL2176855.

Polymorphism and mutation databases

BioMutaiCLEC4C.
DMDMi59797957.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000466151 – 213C-type lectin domain family 4 member CAdd BLAST213

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 82Combined sources1 Publication
Disulfide bondi83 ↔ 94PROSITE-ProRule annotationCombined sources2 Publications
Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi111 ↔ 206PROSITE-ProRule annotationCombined sources2 Publications
Glycosylationi137N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi164N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi180 ↔ 198PROSITE-ProRule annotationCombined sources2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8WTT0.
PRIDEiQ8WTT0.

Expressioni

Tissue specificityi

Expressed in plasmacytoid dendritic cells (PDCs). Constitutively expressed in immature monocyte-derived dendritic cells (iMDDC) and is significantly down-regulated upon maturation with LPS but not with TNF-alpha.3 Publications

Gene expression databases

BgeeiENSG00000198178.
CleanExiHS_CLEC4C.
ExpressionAtlasiQ8WTT0. baseline and differential.
GenevisibleiQ8WTT0. HS.

Organism-specific databases

HPAiHPA029432.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi128012. 10 interactors.
DIPiDIP-29513N.
IntActiQ8WTT0. 3 interactors.
STRINGi9606.ENSP00000353500.

Chemistry databases

BindingDBiQ8WTT0.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi68 – 73Combined sources6
Beta strandi80 – 84Combined sources5
Beta strandi87 – 90Combined sources4
Beta strandi93 – 102Combined sources10
Helixi104 – 113Combined sources10
Helixi124 – 130Combined sources7
Helixi131 – 133Combined sources3
Beta strandi140 – 145Combined sources6
Beta strandi149 – 151Combined sources3
Beta strandi154 – 157Combined sources4
Helixi163 – 165Combined sources3
Beta strandi180 – 186Combined sources7
Turni187 – 189Combined sources3
Beta strandi190 – 196Combined sources7
Beta strandi202 – 209Combined sources8
Beta strandi211 – 213Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WBPX-ray1.80A/B83-210[»]
3WBQX-ray2.30A/B83-210[»]
3WBRX-ray2.20A/B/C/D/E/F/G/H83-210[»]
4ZESX-ray1.65A/B67-213[»]
4ZETX-ray2.90A/B67-213[»]
ProteinModelPortaliQ8WTT0.
SMRiQ8WTT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 207C-type lectinPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 186Carbohydrate bindingCombined sources1 Publication3
Regioni194 – 195Carbohydrate bindingCombined sources1 Publication2

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IS2V. Eukaryota.
ENOG410ZBWX. LUCA.
GeneTreeiENSGT00760000118924.
HOGENOMiHOG000059556.
HOVERGENiHBG050992.
InParanoidiQ8WTT0.
KOiK06723.
OMAiCAIINFR.
OrthoDBiEOG091G0HG1.
PhylomeDBiQ8WTT0.
TreeFamiTF333341.

Family and domain databases

CDDicd03590. CLECT_DC-SIGN_like. 1 hit.
Gene3Di3.10.100.10. 1 hit.
InterProiView protein in InterPro
IPR001304. C-type_lectin-like.
IPR016186. C-type_lectin-like/link.
IPR018378. C-type_lectin_CS.
IPR033989. CD209-like_CTLD.
IPR016187. CTDL_fold.
PfamiView protein in Pfam
PF00059. Lectin_C. 1 hit.
SMARTiView protein in SMART
SM00034. CLECT. 1 hit.
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiView protein in PROSITE
PS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8WTT0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVPEEEPQDR EKGLWWFQLK VWSMAVVSIL LLSVCFTVSS VVPHNFMYSK
60 70 80 90 100
TVKRLSKLRE YQQYHPSLTC VMEGKDIEDW SCCPTPWTSF QSSCYFISTG
110 120 130 140 150
MQSWTKSQKN CSVMGADLVV INTREEQDFI IQNLKRNSSY FLGLSDPGGR
160 170 180 190 200
RHWQWVDQTP YNENVTFWHS GEPNNLDERC AIINFRSSEE WGWNDIHCHV
210
PQKSICKMKK IYI
Length:213
Mass (Da):25,038
Last modified:March 1, 2002 - v1
Checksum:i5DC82C95BE2378C1
GO
Isoform 2 (identifier: Q8WTT0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     11-41: Missing.

Show »
Length:182
Mass (Da):21,498
Checksum:i78EB4E4E0109E4E7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66P → S in AAQ88590 (PubMed:12975309).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01284511 – 41Missing in isoform 2. 1 PublicationAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF325459 mRNA. Translation: AAL37358.1.
AF325460 mRNA. Translation: AAL37359.1.
AF293615 mRNA. Translation: AAL37036.1.
AY358223 mRNA. Translation: AAQ88590.1.
CH471116 Genomic DNA. Translation: EAW88655.1.
CH471116 Genomic DNA. Translation: EAW88656.1.
BC074967 mRNA. Translation: AAH74967.1.
BC074968 mRNA. Translation: AAH74968.1.
BC102015 mRNA. Translation: AAI02016.1.
BC102016 mRNA. Translation: AAI02017.1.
BC102017 mRNA. Translation: AAI02018.1.
BC114338 mRNA. Translation: AAI14339.1.
CCDSiCCDS8583.1. [Q8WTT0-1]
CCDS8584.1. [Q8WTT0-2]
RefSeqiNP_569708.1. NM_130441.2. [Q8WTT0-1]
NP_987099.1. NM_203503.1. [Q8WTT0-2]
UniGeneiHs.351812.

Genome annotation databases

EnsembliENST00000354629; ENSP00000346648; ENSG00000198178. [Q8WTT0-2]
ENST00000360345; ENSP00000353500; ENSG00000198178. [Q8WTT0-1]
ENST00000540085; ENSP00000445338; ENSG00000198178. [Q8WTT0-2]
ENST00000542353; ENSP00000440428; ENSG00000198178. [Q8WTT0-1]
GeneIDi170482.
KEGGihsa:170482.
UCSCiuc001qtg.2. human. [Q8WTT0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCLC4C_HUMAN
AccessioniPrimary (citable) accession number: Q8WTT0
Secondary accession number(s): D3DUU3
, Q3T1C3, Q6UXS8, Q8WXX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: March 1, 2002
Last modified: October 25, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references