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Protein

Rhodopsin kinase

Gene

GRK7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).1 Publication

Catalytic activityi

ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.1 Publication

Enzyme regulationi

Inhibited by phosphorylation of Ser-36.By similarity

Kineticsi

  1. KM=1.9 µM for rhodopsin1 Publication
  2. KM=21.4 µM for ATP1 Publication
  1. Vmax=915 nmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei220ATPPROSITE-ProRule annotation1
Active sitei316Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 205ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • G-protein coupled receptor kinase activity Source: InterPro
  • rhodopsin kinase activity Source: UniProtKB

GO - Biological processi

  • protein autophosphorylation Source: UniProtKB
  • regulation of rhodopsin mediated signaling pathway Source: Reactome
  • signal transduction Source: InterPro
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS03742-MONOMER.
BRENDAi2.7.11.14. 2681.
ReactomeiR-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
SignaLinkiQ8WTQ7.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhodopsin kinase (EC:2.7.11.141 Publication)
Alternative name(s):
G protein-coupled receptor kinase 7
G protein-coupled receptor kinase GRK7
Gene namesi
Name:GRK7
Synonyms:GPRK7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:17031. GRK7.

Subcellular locationi

  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23S → A: No effect on kinase activity. Increase kinase activity; when associated with A-36. 1 Publication1
Mutagenesisi36S → A: No effect on kinase activity. Increase kinase activity; when associated with A-23. 1 Publication1

Organism-specific databases

DisGeNETi131890.
OpenTargetsiENSG00000114124.
PharmGKBiPA38433.

Chemistry databases

ChEMBLiCHEMBL1075133.
GuidetoPHARMACOLOGYi1471.

Polymorphism and mutation databases

BioMutaiGRK7.
DMDMi21263659.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000243321 – 550Rhodopsin kinaseAdd BLAST550
PropeptideiPRO_0000024333551 – 553Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36Phosphoserine; by PKA1 Publication1
Modified residuei550Cysteine methyl esterSequence analysis1
Lipidationi550S-geranylgeranyl cysteineSequence analysis1

Post-translational modificationi

Autophosphorylated in vitro at Ser-490. Phosphorylation at Ser-36 is regulated by light and activated by cAMP.1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiQ8WTQ7.
PeptideAtlasiQ8WTQ7.
PRIDEiQ8WTQ7.

PTM databases

iPTMnetiQ8WTQ7.
PhosphoSitePlusiQ8WTQ7.

Expressioni

Tissue specificityi

Retinal cones, outer and inner segments.2 Publications

Gene expression databases

BgeeiENSG00000114124.
CleanExiHS_GRK7.
GenevisibleiQ8WTQ7. HS.

Interactioni

Subunit structurei

Interacts (when prenylated) with PDE6D; this promotes release from membranes.By similarity

Protein-protein interaction databases

BioGridi126297. 2 interactors.
IntActiQ8WTQ7. 1 interactor.
STRINGi9606.ENSP00000264952.

Chemistry databases

BindingDBiQ8WTQ7.

Structurei

3D structure databases

ProteinModelPortaliQ8WTQ7.
SMRiQ8WTQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 176RGSPROSITE-ProRule annotationAdd BLAST121
Domaini191 – 454Protein kinasePROSITE-ProRule annotationAdd BLAST264
Domaini455 – 520AGC-kinase C-terminalAdd BLAST66

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0986. Eukaryota.
ENOG410YRQZ. LUCA.
GeneTreeiENSGT00860000133699.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ8WTQ7.
KOiK00909.
OMAiESKSHLC.
OrthoDBiEOG091G062G.
PhylomeDBiQ8WTQ7.
TreeFamiTF313940.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8WTQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDMGALDNL IANTAYLQAR KPSDCDSKEL QRRRRSLALP GLQGCAELRQ
60 70 80 90 100
KLSLNFHSLC EQQPIGRRLF RDFLATVPTF RKAATFLEDV QNWELAEEGP
110 120 130 140 150
TKDSALQGLV ATCASAPAPG NPQPFLSQAV ATKCQAATTE EERVAAVTLA
160 170 180 190 200
KAEAMAFLQE QPFKDFVTSA FYDKFLQWKL FEMQPVSDKY FTEFRVLGKG
210 220 230 240 250
GFGEVCAVQV KNTGKMYACK KLDKKRLKKK GGEKMALLEK EILEKVSSPF
260 270 280 290 300
IVSLAYAFES KTHLCLVMSL MNGGDLKFHI YNVGTRGLDM SRVIFYSAQI
310 320 330 340 350
ACGMLHLHEL GIVYRDMKPE NVLLDDLGNC RLSDLGLAVE MKGGKPITQR
360 370 380 390 400
AGTNGYMAPE ILMEKVSYSY PVDWFAMGCS IYEMVAGRTP FKDYKEKVSK
410 420 430 440 450
EDLKQRTLQD EVKFQHDNFT EEAKDICRLF LAKKPEQRLG SREKSDDPRK
460 470 480 490 500
HHFFKTINFP RLEAGLIEPP FVPDPSVVYA KDIAEIDDFS EVRGVEFDDK
510 520 530 540 550
DKQFFKNFAT GAVPIAWQEE IIETGLFEEL NDPNRPTGCE EGNSSKSGVC

LLL
Length:553
Mass (Da):62,212
Last modified:March 1, 2002 - v1
Checksum:iC502E301CF8EB688
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04052781R → H.1 PublicationCorresponds to variant rs34429284dbSNPEnsembl.1
Natural variantiVAR_040528113C → W.1 PublicationCorresponds to variant rs56070798dbSNPEnsembl.1
Natural variantiVAR_040529115S → C.1 PublicationCorresponds to variant rs34769632dbSNPEnsembl.1
Natural variantiVAR_040530127S → T.1 PublicationCorresponds to variant rs35318124dbSNPEnsembl.1
Natural variantiVAR_040531196V → G.1 PublicationCorresponds to variant rs55707760dbSNPEnsembl.1
Natural variantiVAR_040532196V → M.1 PublicationCorresponds to variant rs56019094dbSNPEnsembl.1
Natural variantiVAR_040533226R → W.1 PublicationCorresponds to variant rs35566288dbSNPEnsembl.1
Natural variantiVAR_040534253S → F in a metastatic melanoma sample; somatic mutation. 1 Publication1
Natural variantiVAR_040535309E → Q.1 PublicationCorresponds to variant rs55824414dbSNPEnsembl.1
Natural variantiVAR_040536313V → I.1 PublicationCorresponds to variant rs56076641dbSNPEnsembl.1
Natural variantiVAR_040537443E → G.1 PublicationCorresponds to variant rs36009541dbSNPEnsembl.1
Natural variantiVAR_040538460P → T.1 PublicationCorresponds to variant rs33928105dbSNPEnsembl.1
Natural variantiVAR_051624461R → C.Corresponds to variant rs36004830dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF439409 mRNA. Translation: AAL48216.1.
AF282269 mRNA. Translation: AAL33880.1.
AC112504 Genomic DNA. No translation available.
CCDSiCCDS3120.1.
RefSeqiNP_631948.1. NM_139209.2.
XP_016861206.1. XM_017005717.1.
UniGeneiHs.680654.

Genome annotation databases

EnsembliENST00000264952; ENSP00000264952; ENSG00000114124.
GeneIDi131890.
KEGGihsa:131890.
UCSCiuc011bnd.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF439409 mRNA. Translation: AAL48216.1.
AF282269 mRNA. Translation: AAL33880.1.
AC112504 Genomic DNA. No translation available.
CCDSiCCDS3120.1.
RefSeqiNP_631948.1. NM_139209.2.
XP_016861206.1. XM_017005717.1.
UniGeneiHs.680654.

3D structure databases

ProteinModelPortaliQ8WTQ7.
SMRiQ8WTQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi126297. 2 interactors.
IntActiQ8WTQ7. 1 interactor.
STRINGi9606.ENSP00000264952.

Chemistry databases

BindingDBiQ8WTQ7.
ChEMBLiCHEMBL1075133.
GuidetoPHARMACOLOGYi1471.

PTM databases

iPTMnetiQ8WTQ7.
PhosphoSitePlusiQ8WTQ7.

Polymorphism and mutation databases

BioMutaiGRK7.
DMDMi21263659.

Proteomic databases

PaxDbiQ8WTQ7.
PeptideAtlasiQ8WTQ7.
PRIDEiQ8WTQ7.

Protocols and materials databases

DNASUi131890.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264952; ENSP00000264952; ENSG00000114124.
GeneIDi131890.
KEGGihsa:131890.
UCSCiuc011bnd.3. human.

Organism-specific databases

CTDi131890.
DisGeNETi131890.
GeneCardsiGRK7.
HGNCiHGNC:17031. GRK7.
MIMi606987. gene.
neXtProtiNX_Q8WTQ7.
OpenTargetsiENSG00000114124.
PharmGKBiPA38433.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0986. Eukaryota.
ENOG410YRQZ. LUCA.
GeneTreeiENSGT00860000133699.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ8WTQ7.
KOiK00909.
OMAiESKSHLC.
OrthoDBiEOG091G062G.
PhylomeDBiQ8WTQ7.
TreeFamiTF313940.

Enzyme and pathway databases

BioCyciZFISH:HS03742-MONOMER.
BRENDAi2.7.11.14. 2681.
ReactomeiR-HSA-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
SignaLinkiQ8WTQ7.

Miscellaneous databases

GenomeRNAii131890.
PROiQ8WTQ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114124.
CleanExiHS_GRK7.
GenevisibleiQ8WTQ7. HS.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRK7_HUMAN
AccessioniPrimary (citable) accession number: Q8WTQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.