Reviewed, UniProtKB/Swiss-Prot Q8WTQ7 (GRK7_HUMAN)

Names and origin

Protein names

Recommended name:
    G protein-coupled receptor kinase 7
    EC=2.7.11.16
Alternative name(s):
    G protein-coupled receptor kinase GRK7

Gene names

Name:	GRK7
Synonyms:	GPRK7

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	553 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates cone opsins thereby initiating their deactivation.
Catalytic activity	ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
Subcellular location	Membrane; Lipid-anchor.
Tissue specificity	Retina. Cones and rod.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	G protein-coupled receptor kinase 7		PRO_0000024332
	Propeptide	551 – 553	3	Removed in mature form By similarity		PRO_0000024333
Regions
	Domain	56 – 176	121	RGS
	Domain	191 – 454	264	Protein kinase
	Domain	455 – 520	66	AGC-kinase C-terminal
	Nucleotide binding	197 – 205	9	ATP By similarity
Sites
	Active site	316	1	Proton acceptor By similarity
	Binding site	220	1	ATP By similarity
Amino acid modifications
	Modified residue	550	1	Cysteine methyl ester Potential
	Lipidation	550	1	S-geranylgeranyl cysteine Potential
Natural variations
	Natural variant	81	1	R → H: dbSNP rs34429284. Ref.3		VAR_040527
	Natural variant	113	1	C → W: dbSNP rs56070798. Ref.3		VAR_040528
	Natural variant	115	1	S → C: dbSNP rs34769632. Ref.3		VAR_040529
	Natural variant	127	1	S → T: dbSNP rs35318124. Ref.3		VAR_040530
	Natural variant	196	1	V → G: dbSNP rs55707760. Ref.3		VAR_040531
	Natural variant	196	1	V → M: dbSNP rs56019094. Ref.3		VAR_040532
	Natural variant	226	1	R → W: dbSNP rs35566288. Ref.3		VAR_040533
	Natural variant	253	1	S → F in a metastatic melanoma sample; somatic mutation. Ref.3		VAR_040534
	Natural variant	309	1	E → Q: dbSNP rs55824414. Ref.3		VAR_040535
	Natural variant	313	1	V → I: dbSNP rs56076641. Ref.3		VAR_040536
	Natural variant	443	1	E → G: dbSNP rs36009541. Ref.3		VAR_040537
	Natural variant	460	1	P → T: dbSNP rs33928105. Ref.3		VAR_040538
	Natural variant	461	1	R → C: dbSNP rs36004830.		VAR_051624

Sequences

	Sequence		Length	Mass (Da)
	Q8WTQ7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C502E301CF8EB688	FASTA	553	62,212
	10 20 30 40 50 60 MVDMGALDNL IANTAYLQAR KPSDCDSKEL QRRRRSLALP GLQGCAELRQ KLSLNFHSLC 70 80 90 100 110 120 EQQPIGRRLF RDFLATVPTF RKAATFLEDV QNWELAEEGP TKDSALQGLV ATCASAPAPG 130 140 150 160 170 180 NPQPFLSQAV ATKCQAATTE EERVAAVTLA KAEAMAFLQE QPFKDFVTSA FYDKFLQWKL 190 200 210 220 230 240 FEMQPVSDKY FTEFRVLGKG GFGEVCAVQV KNTGKMYACK KLDKKRLKKK GGEKMALLEK 250 260 270 280 290 300 EILEKVSSPF IVSLAYAFES KTHLCLVMSL MNGGDLKFHI YNVGTRGLDM SRVIFYSAQI 310 320 330 340 350 360 ACGMLHLHEL GIVYRDMKPE NVLLDDLGNC RLSDLGLAVE MKGGKPITQR AGTNGYMAPE 370 380 390 400 410 420 ILMEKVSYSY PVDWFAMGCS IYEMVAGRTP FKDYKEKVSK EDLKQRTLQD EVKFQHDNFT 430 440 450 460 470 480 EEAKDICRLF LAKKPEQRLG SREKSDDPRK HHFFKTINFP RLEAGLIEPP FVPDPSVVYA 490 500 510 520 530 540 KDIAEIDDFS EVRGVEFDDK DKQFFKNFAT GAVPIAWQEE IIETGLFEEL NDPNRPTGCE 550 EGNSSKSGVC LLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of human GRK7 as a potential cone opsin kinase." Chen C.-K., Zhang K., Church-Kopish J., Huang W., Zhang H., Chen Y.-J., Frederick J.M., Baehr W. Mol. Vis. 7:305-313(2001) [PubMed: 11754336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	"Species-specific differences in expression of G-protein-coupled receptor kinase (GRK) 7 and GRK1 in mammalian cone photoreceptor cells: implications for cone cell phototransduction." Weiss E.R., Ducceschi M.H., Horner T.J., Li A., Craft C.M., Osawa S. J. Neurosci. 21:9175-9184(2001) [PubMed: 11717351] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[3]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] HIS-81; TRP-113; CYS-115; THR-127; GLY-196; MET-196; TRP-226; PHE-253; GLN-309; ILE-313; GLY-443 AND THR-460.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF439409 mRNA. Translation: AAL48216.1. AF282269 mRNA. Translation: AAL33880.1.
IPI	IPI00102804.
RefSeq	NP_631948.1.
UniGene	Hs.680654
3D structure databases
SMR	Q8WTQ7. Positions 31-538.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8WTQ7.
PTM databases
PhosphoSite	Q8WTQ7.
Proteomic databases
PRIDE	Q8WTQ7.
Genome annotation databases
Ensembl	ENST00000264952; ENSP00000264952; ENSG00000114124; Homo sapiens. [Genome view]
GeneID	131890.
KEGG	hsa:131890.
UCSC	uc003euf.1. human.
Organism-specific databases
CTD	131890.
GeneCards	GC03P142979.
H-InvDB	HIX0030738.
HGNC	HGNC:17031. GRK7.
MIM	606987. gene.
PharmGKB	PA38433.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG18375.
HOGENOM	HBG716704.
HOVERGEN	Q8WTQ7.
InParanoid	Q8WTQ7.
OMA	WELAEEG.
OrthoDB	EOG9X0QBZ.
PhylomeDB	Q8WTQ7.
Enzyme and pathway databases
BRENDA	2.7.11.14. 247. 2.7.11.16. 247.
Pathway_Interaction_DB	cone_pathway. Visual signal transduction: Cones.
Gene expression databases
ArrayExpress	Q8WTQ7.
Bgee	Q8WTQ7.
CleanEx	HS_GRK7.
Genevestigator	Q8WTQ7.
GermOnline	ENSG00000114124. Homo sapiens.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	82983.
SOURCE	Search...

Entry information

Entry name

GRK7_HUMAN

Accession

Primary (citable) accession number: Q8WTQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	January 19, 2010
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.