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Q8WTQ7 (GRK7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G protein-coupled receptor kinase 7

EC=2.7.11.14
EC=2.7.11.16
Alternative name(s):
G protein-coupled receptor kinase GRK7
Gene names
Name:GRK7
Synonyms:GPRK7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO). Ref.4

Catalytic activity

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate. Ref.4

ATP + [rhodopsin] = ADP + [rhodopsin] phosphate. Ref.4

Enzyme regulation

Inhibited by phosphorylation of Ser-36 By similarity. Ref.4

Subcellular location

Membrane; Lipid-anchor.

Tissue specificity

Retinal cones, outer and inner segments. Ref.1 Ref.5

Post-translational modification

Autophosphorylated in vitro at Ser-490. Phosphorylation at Ser-36 is regulated by light and activated by cAMP. Ref.4 Ref.5

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

Biophysicochemical properties

Kinetic parameters:

KM=1.9 µM for rhodopsin Ref.4

KM=21.4 µM for ATP

Vmax=915 nmol/min/mg enzyme

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 550550G protein-coupled receptor kinase 7
PRO_0000024332
Propeptide551 – 5533Removed in mature form By similarity
PRO_0000024333

Regions

Domain56 – 176121RGS
Domain191 – 454264Protein kinase
Domain455 – 52066AGC-kinase C-terminal
Nucleotide binding197 – 2059ATP By similarity

Sites

Active site3161Proton acceptor By similarity
Binding site2201ATP By similarity

Amino acid modifications

Modified residue361Phosphoserine; by PKA Ref.5
Modified residue5501Cysteine methyl ester Potential
Lipidation5501S-geranylgeranyl cysteine Potential

Natural variations

Natural variant811R → H. Ref.6
Corresponds to variant rs34429284 [ dbSNP | Ensembl ].
VAR_040527
Natural variant1131C → W. Ref.6
Corresponds to variant rs56070798 [ dbSNP | Ensembl ].
VAR_040528
Natural variant1151S → C. Ref.6
Corresponds to variant rs34769632 [ dbSNP | Ensembl ].
VAR_040529
Natural variant1271S → T. Ref.6
Corresponds to variant rs35318124 [ dbSNP | Ensembl ].
VAR_040530
Natural variant1961V → G. Ref.6
Corresponds to variant rs55707760 [ dbSNP | Ensembl ].
VAR_040531
Natural variant1961V → M. Ref.6
Corresponds to variant rs56019094 [ dbSNP | Ensembl ].
VAR_040532
Natural variant2261R → W. Ref.6
Corresponds to variant rs35566288 [ dbSNP | Ensembl ].
VAR_040533
Natural variant2531S → F in a metastatic melanoma sample; somatic mutation. Ref.6
VAR_040534
Natural variant3091E → Q. Ref.6
Corresponds to variant rs55824414 [ dbSNP | Ensembl ].
VAR_040535
Natural variant3131V → I. Ref.6
Corresponds to variant rs56076641 [ dbSNP | Ensembl ].
VAR_040536
Natural variant4431E → G. Ref.6
Corresponds to variant rs36009541 [ dbSNP | Ensembl ].
VAR_040537
Natural variant4601P → T. Ref.6
Corresponds to variant rs33928105 [ dbSNP | Ensembl ].
VAR_040538
Natural variant4611R → C.
Corresponds to variant rs36004830 [ dbSNP | Ensembl ].
VAR_051624

Experimental info

Mutagenesis231S → A: No effect on kinase activity. Increase kinase activity; when associated with A-36. Ref.4
Mutagenesis361S → A: No effect on kinase activity. Increase kinase activity; when associated with A-23. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8WTQ7 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C502E301CF8EB688

FASTA55362,212
        10         20         30         40         50         60 
MVDMGALDNL IANTAYLQAR KPSDCDSKEL QRRRRSLALP GLQGCAELRQ KLSLNFHSLC 

        70         80         90        100        110        120 
EQQPIGRRLF RDFLATVPTF RKAATFLEDV QNWELAEEGP TKDSALQGLV ATCASAPAPG 

       130        140        150        160        170        180 
NPQPFLSQAV ATKCQAATTE EERVAAVTLA KAEAMAFLQE QPFKDFVTSA FYDKFLQWKL 

       190        200        210        220        230        240 
FEMQPVSDKY FTEFRVLGKG GFGEVCAVQV KNTGKMYACK KLDKKRLKKK GGEKMALLEK 

       250        260        270        280        290        300 
EILEKVSSPF IVSLAYAFES KTHLCLVMSL MNGGDLKFHI YNVGTRGLDM SRVIFYSAQI 

       310        320        330        340        350        360 
ACGMLHLHEL GIVYRDMKPE NVLLDDLGNC RLSDLGLAVE MKGGKPITQR AGTNGYMAPE 

       370        380        390        400        410        420 
ILMEKVSYSY PVDWFAMGCS IYEMVAGRTP FKDYKEKVSK EDLKQRTLQD EVKFQHDNFT 

       430        440        450        460        470        480 
EEAKDICRLF LAKKPEQRLG SREKSDDPRK HHFFKTINFP RLEAGLIEPP FVPDPSVVYA 

       490        500        510        520        530        540 
KDIAEIDDFS EVRGVEFDDK DKQFFKNFAT GAVPIAWQEE IIETGLFEEL NDPNRPTGCE 

       550 
EGNSSKSGVC LLL 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of human GRK7 as a potential cone opsin kinase."
Chen C.-K., Zhang K., Church-Kopish J., Huang W., Zhang H., Chen Y.-J., Frederick J.M., Baehr W.
Mol. Vis. 7:305-313(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Retina.
[2]"Species-specific differences in expression of G-protein-coupled receptor kinase (GRK) 7 and GRK1 in mammalian cone photoreceptor cells: implications for cone cell phototransduction."
Weiss E.R., Ducceschi M.H., Horner T.J., Li A., Craft C.M., Osawa S.
J. Neurosci. 21:9175-9184(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Retina.
[3]"The DNA sequence, annotation and analysis of human chromosome 3."
Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. expand/collapse author list , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"Phosphorylation of GRK1 and GRK7 by cAMP-dependent protein kinase attenuates their enzymatic activities."
Horner T.J., Osawa S., Schaller M.D., Weiss E.R.
J. Biol. Chem. 280:28241-28250(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY, FUNCTION IN PHOSPHORYLATION OF RHO, ENZYME REGULATION, AUTOPHOSPHORYLATION, MUTAGENESIS OF SER-23 AND SER-36.
[5]"Phosphorylation of GRK7 by PKA in cone photoreceptor cells is regulated by light."
Osawa S., Jo R., Weiss E.R.
J. Neurochem. 107:1314-1324(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-36.
[6]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] HIS-81; TRP-113; CYS-115; THR-127; GLY-196; MET-196; TRP-226; PHE-253; GLN-309; ILE-313; GLY-443 AND THR-460.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF439409 mRNA. Translation: AAL48216.1.
AF282269 mRNA. Translation: AAL33880.1.
AC112504 Genomic DNA. No translation available.
CCDSCCDS3120.1.
RefSeqNP_631948.1. NM_139209.2.
UniGeneHs.680654.

3D structure databases

ProteinModelPortalQ8WTQ7.
SMRQ8WTQ7. Positions 26-531.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid126297. 2 interactions.
IntActQ8WTQ7. 1 interaction.
STRING9606.ENSP00000264952.

Chemistry

BindingDBQ8WTQ7.
ChEMBLCHEMBL1075133.
GuidetoPHARMACOLOGY1471.

PTM databases

PhosphoSiteQ8WTQ7.

Polymorphism databases

DMDM21263659.

Proteomic databases

PaxDbQ8WTQ7.
PRIDEQ8WTQ7.

Protocols and materials databases

DNASU131890.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000264952; ENSP00000264952; ENSG00000114124.
GeneID131890.
KEGGhsa:131890.
UCSCuc011bnd.2. human.

Organism-specific databases

CTD131890.
GeneCardsGC03P141496.
HGNCHGNC:17031. GRK7.
HPAHPA040138.
MIM606987. gene.
neXtProtNX_Q8WTQ7.
PharmGKBPA38433.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000006742.
HOVERGENHBG004532.
InParanoidQ8WTQ7.
KOK00909.
OMAAYAFESK.
OrthoDBEOG7V1FQK.
PhylomeDBQ8WTQ7.
TreeFamTF313940.

Enzyme and pathway databases

BRENDA2.7.11.14. 2681.
SignaLinkQ8WTQ7.

Gene expression databases

BgeeQ8WTQ7.
CleanExHS_GRK7.
GenevestigatorQ8WTQ7.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR00717. GPCRKINASE.
SMARTSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi131890.
NextBio82983.
PROQ8WTQ7.
SOURCESearch...

Entry information

Entry nameGRK7_HUMAN
AccessionPrimary (citable) accession number: Q8WTQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM