Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8WTQ7

- GRK7_HUMAN

UniProt

Q8WTQ7 - GRK7_HUMAN

Protein

G protein-coupled receptor kinase 7

Gene

GRK7

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).1 Publication

    Catalytic activityi

    ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.1 Publication
    ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.1 Publication

    Enzyme regulationi

    Inhibited by phosphorylation of Ser-36.By similarity

    Kineticsi

    1. KM=1.9 µM for rhodopsin1 Publication
    2. KM=21.4 µM for ATP1 Publication

    Vmax=915 nmol/min/mg enzyme1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei220 – 2201ATPPROSITE-ProRule annotation
    Active sitei316 – 3161Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi197 – 2059ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
    3. rhodopsin kinase activity Source: UniProtKB

    GO - Biological processi

    1. protein autophosphorylation Source: UniProtKB
    2. signal transduction Source: InterPro
    3. termination of G-protein coupled receptor signaling pathway Source: InterPro
    4. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.14. 2681.
    SignaLinkiQ8WTQ7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-coupled receptor kinase 7 (EC:2.7.11.14, EC:2.7.11.16)
    Alternative name(s):
    G protein-coupled receptor kinase GRK7
    Gene namesi
    Name:GRK7
    Synonyms:GPRK7
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 3

    Organism-specific databases

    HGNCiHGNC:17031. GRK7.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi23 – 231S → A: No effect on kinase activity. Increase kinase activity; when associated with A-36. 1 Publication
    Mutagenesisi36 – 361S → A: No effect on kinase activity. Increase kinase activity; when associated with A-23. 1 Publication

    Organism-specific databases

    PharmGKBiPA38433.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 550550G protein-coupled receptor kinase 7PRO_0000024332Add
    BLAST
    Propeptidei551 – 5533Removed in mature formBy similarityPRO_0000024333

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361Phosphoserine; by PKA1 Publication
    Modified residuei550 – 5501Cysteine methyl esterSequence Analysis
    Lipidationi550 – 5501S-geranylgeranyl cysteineSequence Analysis

    Post-translational modificationi

    Autophosphorylated in vitro at Ser-490. Phosphorylation at Ser-36 is regulated by light and activated by cAMP.1 Publication

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Proteomic databases

    PaxDbiQ8WTQ7.
    PRIDEiQ8WTQ7.

    PTM databases

    PhosphoSiteiQ8WTQ7.

    Expressioni

    Tissue specificityi

    Retinal cones, outer and inner segments.2 Publications

    Gene expression databases

    BgeeiQ8WTQ7.
    CleanExiHS_GRK7.
    GenevestigatoriQ8WTQ7.

    Organism-specific databases

    HPAiHPA040138.

    Interactioni

    Protein-protein interaction databases

    BioGridi126297. 2 interactions.
    IntActiQ8WTQ7. 1 interaction.
    STRINGi9606.ENSP00000264952.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8WTQ7.
    SMRiQ8WTQ7. Positions 26-531.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 176121RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini191 – 454264Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini455 – 52066AGC-kinase C-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000006742.
    HOVERGENiHBG004532.
    InParanoidiQ8WTQ7.
    KOiK00909.
    OMAiAYAFESK.
    OrthoDBiEOG7V1FQK.
    PhylomeDBiQ8WTQ7.
    TreeFamiTF313940.

    Family and domain databases

    InterProiIPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR00717. GPCRKINASE.
    SMARTiSM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8WTQ7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVDMGALDNL IANTAYLQAR KPSDCDSKEL QRRRRSLALP GLQGCAELRQ    50
    KLSLNFHSLC EQQPIGRRLF RDFLATVPTF RKAATFLEDV QNWELAEEGP 100
    TKDSALQGLV ATCASAPAPG NPQPFLSQAV ATKCQAATTE EERVAAVTLA 150
    KAEAMAFLQE QPFKDFVTSA FYDKFLQWKL FEMQPVSDKY FTEFRVLGKG 200
    GFGEVCAVQV KNTGKMYACK KLDKKRLKKK GGEKMALLEK EILEKVSSPF 250
    IVSLAYAFES KTHLCLVMSL MNGGDLKFHI YNVGTRGLDM SRVIFYSAQI 300
    ACGMLHLHEL GIVYRDMKPE NVLLDDLGNC RLSDLGLAVE MKGGKPITQR 350
    AGTNGYMAPE ILMEKVSYSY PVDWFAMGCS IYEMVAGRTP FKDYKEKVSK 400
    EDLKQRTLQD EVKFQHDNFT EEAKDICRLF LAKKPEQRLG SREKSDDPRK 450
    HHFFKTINFP RLEAGLIEPP FVPDPSVVYA KDIAEIDDFS EVRGVEFDDK 500
    DKQFFKNFAT GAVPIAWQEE IIETGLFEEL NDPNRPTGCE EGNSSKSGVC 550
    LLL 553
    Length:553
    Mass (Da):62,212
    Last modified:March 1, 2002 - v1
    Checksum:iC502E301CF8EB688
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti81 – 811R → H.1 Publication
    Corresponds to variant rs34429284 [ dbSNP | Ensembl ].
    VAR_040527
    Natural varianti113 – 1131C → W.1 Publication
    Corresponds to variant rs56070798 [ dbSNP | Ensembl ].
    VAR_040528
    Natural varianti115 – 1151S → C.1 Publication
    Corresponds to variant rs34769632 [ dbSNP | Ensembl ].
    VAR_040529
    Natural varianti127 – 1271S → T.1 Publication
    Corresponds to variant rs35318124 [ dbSNP | Ensembl ].
    VAR_040530
    Natural varianti196 – 1961V → G.1 Publication
    Corresponds to variant rs55707760 [ dbSNP | Ensembl ].
    VAR_040531
    Natural varianti196 – 1961V → M.1 Publication
    Corresponds to variant rs56019094 [ dbSNP | Ensembl ].
    VAR_040532
    Natural varianti226 – 2261R → W.1 Publication
    Corresponds to variant rs35566288 [ dbSNP | Ensembl ].
    VAR_040533
    Natural varianti253 – 2531S → F in a metastatic melanoma sample; somatic mutation. 1 Publication
    VAR_040534
    Natural varianti309 – 3091E → Q.1 Publication
    Corresponds to variant rs55824414 [ dbSNP | Ensembl ].
    VAR_040535
    Natural varianti313 – 3131V → I.1 Publication
    Corresponds to variant rs56076641 [ dbSNP | Ensembl ].
    VAR_040536
    Natural varianti443 – 4431E → G.1 Publication
    Corresponds to variant rs36009541 [ dbSNP | Ensembl ].
    VAR_040537
    Natural varianti460 – 4601P → T.1 Publication
    Corresponds to variant rs33928105 [ dbSNP | Ensembl ].
    VAR_040538
    Natural varianti461 – 4611R → C.
    Corresponds to variant rs36004830 [ dbSNP | Ensembl ].
    VAR_051624

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF439409 mRNA. Translation: AAL48216.1.
    AF282269 mRNA. Translation: AAL33880.1.
    AC112504 Genomic DNA. No translation available.
    CCDSiCCDS3120.1.
    RefSeqiNP_631948.1. NM_139209.2.
    UniGeneiHs.680654.

    Genome annotation databases

    EnsembliENST00000264952; ENSP00000264952; ENSG00000114124.
    GeneIDi131890.
    KEGGihsa:131890.
    UCSCiuc011bnd.2. human.

    Polymorphism databases

    DMDMi21263659.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF439409 mRNA. Translation: AAL48216.1 .
    AF282269 mRNA. Translation: AAL33880.1 .
    AC112504 Genomic DNA. No translation available.
    CCDSi CCDS3120.1.
    RefSeqi NP_631948.1. NM_139209.2.
    UniGenei Hs.680654.

    3D structure databases

    ProteinModelPortali Q8WTQ7.
    SMRi Q8WTQ7. Positions 26-531.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 126297. 2 interactions.
    IntActi Q8WTQ7. 1 interaction.
    STRINGi 9606.ENSP00000264952.

    Chemistry

    BindingDBi Q8WTQ7.
    ChEMBLi CHEMBL1075133.
    GuidetoPHARMACOLOGYi 1471.

    PTM databases

    PhosphoSitei Q8WTQ7.

    Polymorphism databases

    DMDMi 21263659.

    Proteomic databases

    PaxDbi Q8WTQ7.
    PRIDEi Q8WTQ7.

    Protocols and materials databases

    DNASUi 131890.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000264952 ; ENSP00000264952 ; ENSG00000114124 .
    GeneIDi 131890.
    KEGGi hsa:131890.
    UCSCi uc011bnd.2. human.

    Organism-specific databases

    CTDi 131890.
    GeneCardsi GC03P141496.
    HGNCi HGNC:17031. GRK7.
    HPAi HPA040138.
    MIMi 606987. gene.
    neXtProti NX_Q8WTQ7.
    PharmGKBi PA38433.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000006742.
    HOVERGENi HBG004532.
    InParanoidi Q8WTQ7.
    KOi K00909.
    OMAi AYAFESK.
    OrthoDBi EOG7V1FQK.
    PhylomeDBi Q8WTQ7.
    TreeFami TF313940.

    Enzyme and pathway databases

    BRENDAi 2.7.11.14. 2681.
    SignaLinki Q8WTQ7.

    Miscellaneous databases

    GenomeRNAii 131890.
    NextBioi 82983.
    PROi Q8WTQ7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8WTQ7.
    CleanExi HS_GRK7.
    Genevestigatori Q8WTQ7.

    Family and domain databases

    InterProi IPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00717. GPCRKINASE.
    SMARTi SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of human GRK7 as a potential cone opsin kinase."
      Chen C.-K., Zhang K., Church-Kopish J., Huang W., Zhang H., Chen Y.-J., Frederick J.M., Baehr W.
      Mol. Vis. 7:305-313(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Retina.
    2. "Species-specific differences in expression of G-protein-coupled receptor kinase (GRK) 7 and GRK1 in mammalian cone photoreceptor cells: implications for cone cell phototransduction."
      Weiss E.R., Ducceschi M.H., Horner T.J., Li A., Craft C.M., Osawa S.
      J. Neurosci. 21:9175-9184(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    3. "The DNA sequence, annotation and analysis of human chromosome 3."
      Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J.
      , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.
      Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "Phosphorylation of GRK1 and GRK7 by cAMP-dependent protein kinase attenuates their enzymatic activities."
      Horner T.J., Osawa S., Schaller M.D., Weiss E.R.
      J. Biol. Chem. 280:28241-28250(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, CATALYTIC ACTIVITY, FUNCTION IN PHOSPHORYLATION OF RHO, ENZYME REGULATION, AUTOPHOSPHORYLATION, MUTAGENESIS OF SER-23 AND SER-36.
    5. "Phosphorylation of GRK7 by PKA in cone photoreceptor cells is regulated by light."
      Osawa S., Jo R., Weiss E.R.
      J. Neurochem. 107:1314-1324(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-36.
    6. "Patterns of somatic mutation in human cancer genomes."
      Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
      , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
      Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS [LARGE SCALE ANALYSIS] HIS-81; TRP-113; CYS-115; THR-127; GLY-196; MET-196; TRP-226; PHE-253; GLN-309; ILE-313; GLY-443 AND THR-460.

    Entry informationi

    Entry nameiGRK7_HUMAN
    AccessioniPrimary (citable) accession number: Q8WTQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 27, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3