Reviewed, UniProtKB/Swiss-Prot Q8WTE7 (OR1_ANOGA)

Names and origin

Protein names

Recommended name:
    Odorant receptor Or1
Alternative name(s):
    AgOr1

Gene names

Name:	OR1
ORF Names:	AGAP009640

Organism

Anopheles gambiae (African malaria mosquito) [Complete proteome]

Taxonomic identifier

7165 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Nematocera › Culicoidea › Culicidae › Anophelinae › Anopheles

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a critical role in the anthropophilic host-seeking behavior; establishes the host preference to transmit malaria. May participate in the phenomenon of decreased host-seeking behavior in disease vector mosquitoes after blood feeding. Ref.1 Ref.4
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Tissue specificity	Female-specific antennas and maxillary palp expression. Ref.1 Ref.5
Induction	Strong response to the odorant 4-methylphenol, a component of human sweat, when expressed in odorant receptor deficient Drosophila. In vivo, decreased expression in antennas after a blood meal. Ref.1 Ref.4
Sequence similarities	Belongs to the odorant-gated ionic channel family.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW olfactory behavior Ref.4 Inferred from direct assay. Source: UniProtKB sensory perception of smell Ref.4 Inferred from direct assay. Source: UniProtKB
Cellular component	integral to membrane Ref.4 Non-traceable author statement. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	odorant binding Ref.4 Inferred from direct assay. Source: UniProtKB olfactory receptor activity Ref.4 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Odorant receptor Or1		PRO_0000174286
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	1 Potential
	Topological domain	24 – 45	22	Extracellular Potential
	Transmembrane	46 – 66	21	2 Potential
	Topological domain	67 – 73	7	Cytoplasmic Potential
	Transmembrane	74 – 94	21	3 Potential
	Topological domain	95 – 133	39	Extracellular Potential
	Transmembrane	134 – 154	21	4 Potential
	Topological domain	155 – 178	24	Cytoplasmic Potential
	Transmembrane	179 – 199	21	5 Potential
	Topological domain	200 – 284	85	Extracellular Potential
	Transmembrane	285 – 305	21	6 Potential
	Topological domain	306 – 317	12	Cytoplasmic Potential
	Transmembrane	318 – 338	21	7 Potential
	Topological domain	339 – 417	79	Extracellular Potential
Amino acid modifications
	Glycosylation	112	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	55	1	M → V in AAR01130. Ref.3
	Sequence conflict	55	1	M → V in AAR01131. Ref.3
	Sequence conflict	55	1	M → V in AAR01132. Ref.3
	Sequence conflict	129	1	Q → R in AAR01130. Ref.3
	Sequence conflict	129	1	Q → R in AAR01131. Ref.3
	Sequence conflict	129	1	Q → R in AAR01132. Ref.3
	Sequence conflict	176	1	H → R in AAR01130. Ref.3
	Sequence conflict	176	1	H → R in AAR01131. Ref.3
	Sequence conflict	176	1	H → R in AAR01132. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8WTE7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: F60D7D7BD93D37F2	FASTA	417	48,520
	10 20 30 40 50 60 MKLNKLNPRW DAYDRRDSFW LQLLCLKYLG LWPPEDTDQA TRNRYIAYGW ALRIMFLHLY 70 80 90 100 110 120 ALTQALYFKD VKDINDIANA LFVLMTQVTL IYKLEKFNYN IARIQACLRK LNCTLYHPKQ 130 140 150 160 170 180 REEFSPVLQS MSGVFWLMIF LMFVAIFTII MWVMSPAFDN ERRLPVPAWF PVDYHHSDIV 190 200 210 220 230 240 YGVLFLYQTI GIVMSATYNF STDTMFSGLM LHINGQIVRL GSMVKKLGHD VPPERQLVAT 250 260 270 280 290 300 DAEWKEMRKR IDHHSKVYGT MYAKVTECVL FHKDILSFGD EVQDIFQGSI FAQVCASVII 310 320 330 340 350 360 ICMTLLQATG DDVTMADLLG CGFYLLVMTS QVFIFCYVGN EISYTTDKFT EFVGFSNYFK 370 380 390 400 410 FDKRTSQAMI FFLQMTLKDV HIKVGSVLKV TLNLHTFLQI MKLSYSYLAV LQSMESE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Candidate odorant receptors from the malaria vector mosquito Anopheles gambiae and evidence of down-regulation in response to blood feeding." Fox A.N., Pitts R.J., Robertson H.M., Carlson J.R., Zwiebel L.J. Proc. Natl. Acad. Sci. U.S.A. 98:14693-14697(2001) [PubMed: 11724964] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY. Strain: G3.
[2]	"The genome sequence of the malaria mosquito Anopheles gambiae." Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R., Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R., Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z., Kraft C.L., Abril J.F. , Anthouard V., Arensburger P., Atkinson P.W., Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C., Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K., Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V., Dana A., Delcher A., Dew I., Evans C.A., Flanigan M., Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R., Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J., Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I., Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A., McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D., O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H., Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J., Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B., Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M., Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I., Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J., Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M., Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C., Collins F.H., Hoffman S.L. Science 298:129-149(2002) [PubMed: 12364791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PEST.
[3]	"Intraspecific nucleotide variation in Anopheles gambiae: new insights into the biology of malaria vectors." Morlais I., Poncon N., Simard F., Cohuet A., Fontenille D. Am. J. Trop. Med. Hyg. 71:795-802(2004) [PubMed: 15642974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 35-236. Strain: 4aRR, L3-5 and Yaounde.
[4]	"Olfaction: mosquito receptor for human-sweat odorant." Hallem E.A., Fox A.N., Zwiebel L.J., Carlson J.R. Nature 427:212-213(2004) [PubMed: 14724626] [Abstract] Cited for: FUNCTION, INDUCTION.
[5]	"G protein-coupled receptors in Anopheles gambiae." Hill C.A., Fox A.N., Pitts R.J., Kent L.B., Tan P.L., Chrystal M.A., Cravchik A., Collins F.H., Robertson H.M., Zwiebel L.J. Science 298:176-178(2002) [PubMed: 12364795] [Abstract] Cited for: IDENTIFICATION, TISSUE SPECIFICITY.

Web resources

Protein Spotlight

What mosquitoes sniff - Issue 44 of March 2004

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF364130 Genomic DNA. Translation: AAL35506.1. AAAB01008980 Genomic DNA. Translation: EAA13838.1. AY334005 Genomic DNA. Translation: AAR01130.1. AY334006 Genomic DNA. Translation: AAR01131.1. AY334007 Genomic DNA. Translation: AAR01132.1.
RefSeq	XP_318674.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1279017.
KEGG	aga:AgaP_AGAP009640.
VectorBase	AGAP009639. Anopheles gambiae.
Organism-specific databases
CTD	1279017.
Phylogenomic databases
InParanoid	Q8WTE7.
OrthoDB	EOG9CG0FJ.
PhylomeDB	Q8WTE7.
Family and domain databases
InterPro	IPR004117. 7tm6_olfct_rcpt. [Graphical view]
PANTHER	PTHR21137. 7tm6_olfct_rcpt. 1 hit.
Pfam	PF02949. 7tm_6. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OR1_ANOGA

Accession

Primary (citable) accession number: Q8WTE7
Secondary accession number(s): Q6VPM5, Q7Q1T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2002
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)