Q8WSW3 (MTCD_TETTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cadmium metallothionein

Alternative name(s):
Cd-MT
MT-Cd

Gene names

Name:

MTT1

Organism

Tetrahymena thermophila

Taxonomic identifier

5911 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Alveolata › Ciliophora › Intramacronucleata › Oligohymenophorea › Hymenostomatida › Tetrahymenina › Tetrahymenidae › Tetrahymena

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The metallothioneins are involved in the cellular sequestration of toxic metal ions.
Induction	By cadmium, and to a lesser extent by mercury, copper, heat, hydrogen peroxide and IL-6. Ref.1 Ref.5
Sequence similarities	Belongs to the metallothionein superfamily. Type 7 family.
Mass spectrometry	Molecular mass is 16763 Da from positions 3 - 162. Determined by MALDI. Ref.5

Ontologies

Keywords
Ligand	Cadmium Metal-binding Metal-thiolate cluster
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	cadmium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 2	2			PRO_0000018673
	Chain	3 – 162	160	Cadmium metallothionein		PRO_0000018674
Experimental info
	Sequence conflict	6	1	S → NN in AAP33500. Ref.4
	Sequence conflict	156	1	Q → K in AAP33500. Ref.4
	Sequence conflict	159	1	Q → E in AAP33500. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8WSW3 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 9B2DB3C95981FFCD	FASTA	162	16,757
	10 20 30 40 50 60 MDKVNSCCCG VNAKPCCTDP NSGCCCVSKT DNCCKSDTKE CCTGTGEGCK CVNCKCCKPQ 70 80 90 100 110 120 ANCCCGVNAK PCCFDPNSGC CCVSKTNNCC KSDTKECCTG TGEGCKCTSC QCCKPVQQGC 130 140 150 160 CCGDKAKACC TDPNSGCCCS NKANKCCDAT SKQECQTCQC CK

References

[1]	"A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila." Shang Y., Song X., Bowen J., Corstanje R., Gao Y., Gaertig J., Gorovsky M.A. Proc. Natl. Acad. Sci. U.S.A. 99:3734-3739(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: CU428.
[2]	"Tetrahymena metallothioneins fall into two discrete subfamilies." Diaz S., Amaro F., Rico D., Campos V., Benitez L., Martin-Gonzalez A., Hamilton E.P., Orias E., Gutierrez J.C. PLoS ONE 2:E291-E291(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: Inbred strain B and SB1969.
[3]	"Evolution of metallothioneins in Tetrahymena thermophila." Formigari A., Santovito G., Boldrin F., Irato P., Piccinni E. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Inbred B.
[4]	"Tetrahymena thermophila cadmium metallothionein gene." Miao W., Wan M., Song Y. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-162.
[5]	"Biochemical characterization and quantitative gene expression analysis of the multi-stress inducible metallothionein from Tetrahymena thermophila." Dondero F., Cavaletto M., Ghezzi A.R., La Terza A., Banni M., Viarengo A. Protist 155:157-168(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-162, PROTEIN SEQUENCE OF 3-17, INDUCTION, MASS SPECTROMETRY. Strain: CU428.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY061892 Genomic DNA. Translation: AAL31645.1. AY740525 Genomic DNA. Translation: AAU87360.1. DQ517937 mRNA. Translation: ABF68755.1. EF195745 mRNA. Translation: ABN42648.1. DQ022186 mRNA. Translation: AAY42865.1. AY273793 mRNA. Translation: AAP33500.1. AF537326 mRNA. Translation: AAN06822.1.
RefSeq	XP_001024888.1. XM_001024888.1.
UniGene	Tth.4002.
3D structure databases
ProteinModelPortal	Q8WSW3.
ModBase	Search...
Protein-protein interaction databases
STRING	5911.Q8WSW3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7828336.
KEGG	tet:TTHERM_00241640.
Phylogenomic databases
eggNOG	NOG85154.
ProtClustDB	CLSZ2499415.
Family and domain databases
InterPro	IPR012484. Metalthion_7. [Graphical view]
Pfam	PF07846. Metallothio_7. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTCD_TETTH

Accession

Primary (citable) accession number: Q8WSW3
Secondary accession number(s): Q5XQF6, Q86BU0, Q8IT78

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)