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Protein

Cadmium metallothionein

Gene

MTT1

Organism
Tetrahymena thermophila
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The metallothioneins are involved in the cellular sequestration of toxic metal ions.

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Cadmium, Metal-binding, Metal-thiolate cluster

Names & Taxonomyi

Protein namesi
Recommended name:
Cadmium metallothionein
Alternative name(s):
Cd-MT
MT-Cd
Gene namesi
Name:MTT1
OrganismiTetrahymena thermophila
Taxonomic identifieri5911 [NCBI]
Taxonomic lineageiEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 221 PublicationPRO_0000018673
Chaini3 – 162160Cadmium metallothioneinPRO_0000018674Add
BLAST

Expressioni

Inductioni

By cadmium, and to a lesser extent by mercury, copper, heat, hydrogen peroxide and IL-6.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi5911.EAS04643.

Structurei

3D structure databases

ProteinModelPortaliQ8WSW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG85154.

Family and domain databases

InterProiIPR012484. Metalthion_7.
[Graphical view]
PfamiPF07846. Metallothio_Cad. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8WSW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKVNSCCCG VNAKPCCTDP NSGCCCVSKT DNCCKSDTKE CCTGTGEGCK
60 70 80 90 100
CVNCKCCKPQ ANCCCGVNAK PCCFDPNSGC CCVSKTNNCC KSDTKECCTG
110 120 130 140 150
TGEGCKCTSC QCCKPVQQGC CCGDKAKACC TDPNSGCCCS NKANKCCDAT
160
SKQECQTCQC CK
Length:162
Mass (Da):16,757
Last modified:March 1, 2002 - v1
Checksum:i9B2DB3C95981FFCD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61S → NN in AAP33500 (Ref. 4) Curated
Sequence conflicti156 – 1561Q → K in AAP33500 (Ref. 4) Curated
Sequence conflicti159 – 1591Q → E in AAP33500 (Ref. 4) Curated

Mass spectrometryi

Molecular mass is 16763 Da from positions 3 - 162. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061892 Genomic DNA. Translation: AAL31645.1.
AY740525 Genomic DNA. Translation: AAU87360.1.
DQ517937 mRNA. Translation: ABF68755.1.
EF195745 mRNA. Translation: ABN42648.1.
DQ022186 mRNA. Translation: AAY42865.1.
AY273793 mRNA. Translation: AAP33500.1.
AF537326 mRNA. Translation: AAN06822.1.
RefSeqiXP_001024888.1. XM_001024888.1.
UniGeneiTth.4002.

Genome annotation databases

GeneIDi7828336.
KEGGitet:TTHERM_00241640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY061892 Genomic DNA. Translation: AAL31645.1.
AY740525 Genomic DNA. Translation: AAU87360.1.
DQ517937 mRNA. Translation: ABF68755.1.
EF195745 mRNA. Translation: ABN42648.1.
DQ022186 mRNA. Translation: AAY42865.1.
AY273793 mRNA. Translation: AAP33500.1.
AF537326 mRNA. Translation: AAN06822.1.
RefSeqiXP_001024888.1. XM_001024888.1.
UniGeneiTth.4002.

3D structure databases

ProteinModelPortaliQ8WSW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5911.EAS04643.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi7828336.
KEGGitet:TTHERM_00241640.

Phylogenomic databases

eggNOGiNOG85154.

Family and domain databases

InterProiIPR012484. Metalthion_7.
[Graphical view]
PfamiPF07846. Metallothio_Cad. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila."
    Shang Y., Song X., Bowen J., Corstanje R., Gao Y., Gaertig J., Gorovsky M.A.
    Proc. Natl. Acad. Sci. U.S.A. 99:3734-3739(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: CU428.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Inbred strain B and SB1969.
  3. "Evolution of metallothioneins in Tetrahymena thermophila."
    Formigari A., Santovito G., Boldrin F., Irato P., Piccinni E.
    Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Inbred B.
  4. "Tetrahymena thermophila cadmium metallothionein gene."
    Miao W., Wan M., Song Y.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-162.
  5. "Biochemical characterization and quantitative gene expression analysis of the multi-stress inducible metallothionein from Tetrahymena thermophila."
    Dondero F., Cavaletto M., Ghezzi A.R., La Terza A., Banni M., Viarengo A.
    Protist 155:157-168(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-162, PROTEIN SEQUENCE OF 3-17, INDUCTION, MASS SPECTROMETRY.
    Strain: CU428.

Entry informationi

Entry nameiMTCD_TETTH
AccessioniPrimary (citable) accession number: Q8WSW3
Secondary accession number(s): Q5XQF6, Q86BU0, Q8IT78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: March 1, 2002
Last modified: June 24, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.