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Q8WSW3 (MTCD_TETTH) Reviewed, UniProtKB/Swiss-Prot

Last modified September 18, 2013. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cadmium metallothionein
Alternative name(s):
Cd-MT
MT-Cd
Gene names
Name:MTT1
OrganismTetrahymena thermophila
Taxonomic identifier5911 [NCBI]
Taxonomic lineageEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

Protein attributes

Sequence length162 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The metallothioneins are involved in the cellular sequestration of toxic metal ions.

Induction

By cadmium, and to a lesser extent by mercury, copper, heat, hydrogen peroxide and IL-6. Ref.1 Ref.5

Sequence similarities

Belongs to the metallothionein superfamily. Type 7 family.

Mass spectrometry

Molecular mass is 16763 Da from positions 3 - 162. Determined by MALDI. Ref.5

Ontologies

Keywords
   LigandCadmium
Metal-binding
Metal-thiolate cluster
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Molecular_functioncadmium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 22
PRO_0000018673
Chain3 – 162160Cadmium metallothionein
PRO_0000018674

Experimental info

Sequence conflict61S → NN in AAP33500. Ref.4
Sequence conflict1561Q → K in AAP33500. Ref.4
Sequence conflict1591Q → E in AAP33500. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8WSW3 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 9B2DB3C95981FFCD

FASTA16216,757
        10         20         30         40         50         60 
MDKVNSCCCG VNAKPCCTDP NSGCCCVSKT DNCCKSDTKE CCTGTGEGCK CVNCKCCKPQ 

        70         80         90        100        110        120 
ANCCCGVNAK PCCFDPNSGC CCVSKTNNCC KSDTKECCTG TGEGCKCTSC QCCKPVQQGC 

       130        140        150        160 
CCGDKAKACC TDPNSGCCCS NKANKCCDAT SKQECQTCQC CK 

« Hide

References

[1]"A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila."
Shang Y., Song X., Bowen J., Corstanje R., Gao Y., Gaertig J., Gorovsky M.A.
Proc. Natl. Acad. Sci. U.S.A. 99:3734-3739(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
Strain: CU428.
[2]"Tetrahymena metallothioneins fall into two discrete subfamilies."
Diaz S., Amaro F., Rico D., Campos V., Benitez L., Martin-Gonzalez A., Hamilton E.P., Orias E., Gutierrez J.C.
PLoS ONE 2:E291-E291(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: Inbred strain B and SB1969.
[3]"Evolution of metallothioneins in Tetrahymena thermophila."
Formigari A., Santovito G., Boldrin F., Irato P., Piccinni E.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Inbred B.
[4]"Tetrahymena thermophila cadmium metallothionein gene."
Miao W., Wan M., Song Y.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-162.
[5]"Biochemical characterization and quantitative gene expression analysis of the multi-stress inducible metallothionein from Tetrahymena thermophila."
Dondero F., Cavaletto M., Ghezzi A.R., La Terza A., Banni M., Viarengo A.
Protist 155:157-168(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-162, PROTEIN SEQUENCE OF 3-17, INDUCTION, MASS SPECTROMETRY.
Strain: CU428.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY061892 Genomic DNA. Translation: AAL31645.1.
AY740525 Genomic DNA. Translation: AAU87360.1.
DQ517937 mRNA. Translation: ABF68755.1.
EF195745 mRNA. Translation: ABN42648.1.
DQ022186 mRNA. Translation: AAY42865.1.
AY273793 mRNA. Translation: AAP33500.1.
AF537326 mRNA. Translation: AAN06822.1.
RefSeqXP_001024888.1. XM_001024888.1.
UniGeneTth.4002.

3D structure databases

ProteinModelPortalQ8WSW3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5911.Q8WSW3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7828336.
KEGGtet:TTHERM_00241640.

Phylogenomic databases

eggNOGNOG85154.
ProtClustDBCLSZ2499415.

Family and domain databases

InterProIPR012484. Metalthion_7.
[Graphical view]
PfamPF07846. Metallothio_Cad. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTCD_TETTH
AccessionPrimary (citable) accession number: Q8WSW3
Secondary accession number(s): Q5XQF6, Q86BU0, Q8IT78
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: March 1, 2002
Last modified: September 18, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Metallothioneins

Classification of metallothioneins and list of entries