Reviewed, UniProtKB/Swiss-Prot Q8WSR7 (CTNB_DICDI)

Names and origin

Protein names

Recommended name:
    Countin-2

Gene names

Name:	ctnB
ORF Names:	DDB_G0279797

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cell-counting factor that limits the minimum size of the multicellular structure. May up-regulate the expression of both gp24 and gp80, which mediate cell adhesion. Ref.1
Subcellular location	Secreted Ref.1.
Developmental stage	Expressed in the vegetative cells. Increases during aggregation to mound stages (6-12 hours) and decreases at late developmental stages (18-24 hours). Ref.1
Disruption phenotype	Cells form a higher number of smaller aggregates. Ref.1
Sequence similarities	Belongs to the countin family. Contains 1 saposin B-type domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aggregation involved in sorocarp development Ref.1 Inferred from mutant phenotype. Source: dictyBase cell-cell adhesion Ref.1 Inferred from mutant phenotype. Source: dictyBase
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 254	235	Countin-2		PRO_0000327909
Regions
	Domain	22 – 107	86	Saposin B-type
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) Potential
	Glycosylation	219	1	N-linked (GlcNAc...) Potential
	Disulfide bond	26 ↔ 103		By similarity
	Disulfide bond	29 ↔ 97		By similarity
	Disulfide bond	56 ↔ 68		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8WSR7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 359482BE25C6D3AF	FASTA	254	26,347
	10 20 30 40 50 60 MMIKYITIAI LFIASLVKAD LQFSLCPTCV DFINNDMGDL EKIISGGIAT SCGAVCSLLP 70 80 90 100 110 120 NNIEQGACNL LCDIVGIDEF LKVFNNIGED ADPVWICEEL TVCPKNQNSN ATVLTSDVSP 130 140 150 160 170 180 ASGPHGTTFT IGVAYKVEST LGTGEVAVMV TDPTGSNGFG DAQLIVNTQP GQYSTSFSFA 190 200 210 220 230 240 ATPSEDEQFP AGVYQVQLMI CEGSCGAKHS VTFNSVYANF TVTSGPSVTG QMTGTGSGSG 250 SGSGSSSGAA YLRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two cell-counting factors regulate the aggregate size of the cellular slime mold Dictyostelium discoideum." Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H. Dev. Growth Differ. 43:735-744(2001) [PubMed: 11737154] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB067573 mRNA. Translation: BAB84187.1. AAFI02000033 Genomic DNA. Translation: EAL67512.1.
RefSeq	XP_641512.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	8622253.
KEGG	ddi:DDB_0214993.
Organism-specific databases
dictyBase	DDB_G0279797. ctnB.
Phylogenomic databases
HOGENOM	HBG077942.
Family and domain databases
InterPro	IPR008139. SaposinB. [Graphical view]
SMART	SM00741. SapB. 1 hit. [Graphical view]
PROSITE	PS50015. SAP_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTNB_DICDI

Accession

Primary (citable) accession number: Q8WSR7
Secondary accession number(s): Q54W80

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)