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Q8WSR7 (CTNB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Countin-2
Gene names
Name:ctnB
ORF Names:DDB_G0279797
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cell-counting factor that limits the minimum size of the multicellular structure. May up-regulate the expression of both gp24 and gp80, which mediate cell adhesion. Ref.1

Subcellular location

Secreted Ref.1.

Developmental stage

Expressed in the vegetative cells. Increases during aggregation to mound stages (6-12 hours) and decreases at late developmental stages (18-24 hours). Ref.1

Disruption phenotype

Cells form a higher number of smaller aggregates. Ref.1

Sequence similarities

Belongs to the countin family.

Contains 1 saposin B-type domain.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaggregation involved in sorocarp development

Inferred from mutant phenotype Ref.1. Source: dictyBase

cell-cell adhesion

Inferred from mutant phenotype Ref.1. Source: dictyBase

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 254235Countin-2
PRO_0000327909

Regions

Domain22 – 10786Saposin B-type

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) Potential
Glycosylation2191N-linked (GlcNAc...) Potential
Disulfide bond26 ↔ 103 By similarity
Disulfide bond29 ↔ 97 By similarity
Disulfide bond56 ↔ 68 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8WSR7 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 359482BE25C6D3AF

FASTA25426,347
        10         20         30         40         50         60 
MMIKYITIAI LFIASLVKAD LQFSLCPTCV DFINNDMGDL EKIISGGIAT SCGAVCSLLP 

        70         80         90        100        110        120 
NNIEQGACNL LCDIVGIDEF LKVFNNIGED ADPVWICEEL TVCPKNQNSN ATVLTSDVSP 

       130        140        150        160        170        180 
ASGPHGTTFT IGVAYKVEST LGTGEVAVMV TDPTGSNGFG DAQLIVNTQP GQYSTSFSFA 

       190        200        210        220        230        240 
ATPSEDEQFP AGVYQVQLMI CEGSCGAKHS VTFNSVYANF TVTSGPSVTG QMTGTGSGSG 

       250 
SGSGSSSGAA YLRY 

« Hide

References

« Hide 'large scale' references
[1]"Two cell-counting factors regulate the aggregate size of the cellular slime mold Dictyostelium discoideum."
Okuwa T., Katayama T., Takano A., Kodaira K., Yasukawa H.
Dev. Growth Differ. 43:735-744(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[2]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB067573 mRNA. Translation: BAB84187.1.
AAFI02000033 Genomic DNA. Translation: EAL67512.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0214993.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0214993; DDB0214993; DDB_G0279797.
KEGGddi:DDB_G0279797.

Organism-specific databases

dictyBaseDDB_G0279797. ctnB.

Phylogenomic databases

eggNOGNOG12793.
OMASGPHGTT.
PhylomeDBQ8WSR7.
ProtClustDBCLSZ2430664.

Family and domain databases

InterProIPR008139. SaposinB.
[Graphical view]
SMARTSM00741. SapB. 1 hit.
[Graphical view]
PROSITEPS50015. SAP_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCTNB_DICDI
AccessionPrimary (citable) accession number: Q8WSR7
Secondary accession number(s): Q54W80
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase