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Q8WSF8 (Q8WSF8_APLCA) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names
OrganismAplysia californica (California sea hare) EMBL AAL37251.1
Taxonomic identifier6500 [NCBI]
Taxonomic lineageEukaryotaMetazoaLophotrochozoaMolluscaGastropodaHeterobranchiaEuthyneuraEuopisthobranchiaAplysiomorphaAplysioideaAplysiidaeAplysia

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region20 – 223Sulfate 1 binding PDB 2Y54 PDB 2Y56
Region78 – 803Sulfate 4 binding PDB 3GUA PDB 2Y56
Region94 – 963Sulfate 2 binding PDB 3GUA
Region94 – 963Sulfate 8 binding PDB 3GUA
Region167 – 1704Phosphate 1 binding
Region167 – 1704Phosphate 2 binding
Region167 – 1704Sulfate 3 binding PDB 3GUA
Region186 – 1883Sulfate 17 binding PDB 2Y57
Region186 – 1883Sulfate 18 binding PDB 2Y54 PDB 2Y56 PDB 2Y57 PDB 2Y58
Region201 – 2033Sulfate 13 binding PDB 2Y56 PDB 2Y57 PDB 2Y58

Sites

Metal binding1671Magnesium 1 PDB 3PMZ
Metal binding1671Magnesium 2 PDB 3PMZ
Metal binding1701Magnesium 2 PDB 3PMZ
Metal binding2101Magnesium 1 PDB 3PMZ
Binding site201Chloride 1; via amide nitrogen PDB 4BQT
Binding site421Phosphate 1
Binding site421Phosphate 2
Binding site421Sulfate 3 PDB 3GUA
Binding site551Sulfate 5 PDB 2Y56
Binding site591Phosphate 3 PDB 2XZ5
Binding site591Phosphate 4 PDB 2XZ5
Binding site591Phosphate 5 PDB 2XZ5
Binding site591Sulfate 6 PDB 3GUA
Binding site591Sulfate 7 PDB 3GUA
Binding site681Phosphate 3 PDB 2XZ5
Binding site761Phosphate 6 PDB 2YME
Binding site961Phosphate 1
Binding site961Phosphate 7
Binding site1031Sulfate 9 PDB 3GUA
Binding site1101Cocaine PDB 2PGZ
Binding site1141Chloride 3 PDB 4BQT
Binding site1141Phosphate 3 PDB 2XZ5
Binding site1141Phosphate 4 PDB 2XZ5
Binding site1141Phosphate 8 PDB 2XZ5
Binding site1141Sulfate 6 PDB 3GUA
Binding site1221Chloride 2; via amide nitrogen
Binding site1221Chloride 4; via amide nitrogen PDB 2Y54
Binding site1221Sulfate 10; via amide nitrogen PDB 2Y57 PDB 2Y58
Binding site1221Sulfate 11; via amide nitrogen PDB 2Y57
Binding site1411Phosphate 3 PDB 2XZ5
Binding site1451Sulfate 11; via amide nitrogen PDB 2Y57
Binding site1451Sulfate 12; via amide nitrogen PDB 2Y54
Binding site1451Sulfate 13; via amide nitrogen PDB 2Y56 PDB 2Y58
Binding site1451Sulfate 14; via amide nitrogen PDB 2Y56
Binding site1451Sulfate 15; via amide nitrogen PDB 2Y56 PDB 2Y58
Binding site1451Sulfate 16; via amide nitrogen PDB 2Y57
Binding site1451Sulfate 17; via amide nitrogen PDB 2Y57
Binding site1451Sulfate 1; via amide nitrogen PDB 2Y56
Binding site1601Sulfate 13 PDB 2Y56 PDB 2Y57
Binding site1601Sulfate 14 PDB 2Y56
Binding site1641Cocaine PDB 2PGZ
Binding site1661Sulfate 9 PDB 3GUA
Binding site1701Sulfate 2 PDB 3GUA
Binding site1841Sulfate 5; via carbonyl oxygen PDB 2Y56
Binding site2011Sulfate 14 PDB 2Y56
Binding site2031Sulfate 17 PDB 2Y57
Binding site2051Cocaine PDB 2PGZ
Binding site2071Sulfate 19; via amide nitrogen PDB 2BR8
Binding site2071Sulfate 20 PDB 2BR8
Binding site2101Phosphate 1
Binding site2101Phosphate 2
Binding site2121Cocaine PDB 2PGZ
Binding site2161Sulfate 17 PDB 2Y57

Amino acid modifications

Glycosylation911N-linked (GlcNAc...) PDB 2PGZ PDB 2BYN PDB 2BYP PDB 2WN9 PDB 2WNJ PDB 2WNL PDB 3SH1 PDB 3SIO PDB 4DBM PDB 2YME

Sequences

Sequence LengthMass (Da)Tools
Q8WSF8 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 6D0DE487C46707BE

FASTA23626,568
        10         20         30         40         50         60 
MLVSVYLALL VACVGQAHSQ ANLMRLKSDL FNRSPMYPGP TKDDPLTVTL GFTLQDIVKA 

        70         80         90        100        110        120 
DSSTNEVDLV YYEQQRWKLN SLMWDPNEYG NITDFRTSAA DIWTPDITAY SSTRPVQVLS 

       130        140        150        160        170        180 
PQIAVVTHDG SVMFIPAQRL SFMCDPTGVD SEEGATCAVK FGSWVYSGFE IDLKTDTDQV 

       190        200        210        220        230 
DLSSYYASSK YEILSATQTR QVQHYSCCPE PYIDVNLVVK FRERRAGNGF FRNLFD 

« Hide

References

[1]"Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations."
Hansen S.B., Sulzenbacher G., Huxford T., Marchot P., Taylor P., Bourne Y.
EMBO J. 24:3635-3646(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 18-236, GLYCOSYLATION AT ASN-91.
[2]"Crystal structure of nicotinic acetylcholine receptor homolog AChBP in complex with an alpha-conotoxin PnIA variant."
Celie P.H., Kasheverov I.E., Mordvintsev D.Y., Hogg R.C., van Nierop P., van Elk R., van Rossum-Fikkert S.E., Zhmak M.N., Bertrand D., Tsetlin V., Sixma T.K., Smit A.B.
Nat. Struct. Mol. Biol. 12:582-588(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236 IN COMPLEX WITH SULFATE.
[3]"Neuronal transcriptome of Aplysia: neuronal compartments and circuitry."
Moroz L.L., Edwards J.R., Puthanveettil S.V., Kohn A.B., Ha T., Heyland A., Knudsen B., Sahni A., Yu F., Liu L., Jezzini S., Lovell P., Iannucculli W., Chen M., Nguyen T., Sheng H., Shaw R., Kalachikov S. expand/collapse author list , Panchin Y.V., Farmerie W., Russo J.J., Ju J., Kandel E.R.
Cell 127:1453-1467(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[4]"Structural determinants of selective alpha-conotoxin binding to a nicotinic acetylcholine receptor homolog AChBP."
Ulens C., Hogg R.C., Celie P.H., Bertrand D., Tsetlin V., Smit A.B., Sixma T.K.
Proc. Natl. Acad. Sci. U.S.A. 103:3615-3620(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 20-236.
[5]"AChBP-targeted alpha-conotoxin correlates distinct binding orientations with nAChR subtype selectivity."
Dutertre S., Ulens C., Buttner R., Fish A., van Elk R., Kendel Y., Hopping G., Alewood P.F., Schroeder C., Nicke A., Smit A.B., Sixma T.K., Lewis R.J.
EMBO J. 26:3858-3867(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236.
[6]"Galanthamine and non-competitive inhibitor binding to ACh-binding protein: evidence for a binding site on non-alpha-subunit interfaces of heteromeric neuronal nicotinic receptors."
Hansen S.B., Taylor P.
J. Mol. Biol. 369:895-901(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 18-236 IN COMPLEX WITH COCAINE, GLYCOSYLATION AT ASN-91.
[7]"An ion selectivity filter in the extracellular domain of Cys-loop receptors reveals determinants for ion conductance."
Hansen S.B., Wang H.L., Taylor P., Sine S.M.
J. Biol. Chem. 283:36066-36070(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 18-225 IN COMPLEX WITH SULFATE.
[8]"Atomic interactions of neonicotinoid agonists with AChBP: molecular recognition of the distinctive electronegative pharmacophore."
Talley T.T., Harel M., Hibbs R.E., Radic Z., Tomizawa M., Casida J.E., Taylor P.
Proc. Natl. Acad. Sci. U.S.A. 105:7606-7611(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 18-236.
[9]"Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal alpha7 nicotinic acetylcholine receptor."
Hibbs R.E., Sulzenbacher G., Shi J., Talley T.T., Conrod S., Kem W.R., Taylor P., Marchot P., Bourne Y.
EMBO J. 28:3040-3051(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 18-236, GLYCOSYLATION AT ASN-91.
[10]"Use of acetylcholine binding protein in the search for novel alpha7 nicotinic receptor ligands. In silico docking, pharmacological screening, and X-ray analysis."
Ulens C., Akdemir A., Jongejan A., van Elk R., Bertrand S., Perrakis A., Leurs R., Smit A.B., Sixma T.K., Bertrand D., de Esch I.J.
J. Med. Chem. 52:2372-2383(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 20-236.
[11]"Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism."
Bourne Y., Radic Z., Araoz R., Talley T.T., Benoit E., Servent D., Taylor P., Molgo J., Marchot P.
Proc. Natl. Acad. Sci. U.S.A. 107:6076-6081(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 18-236.
[12]"The curare alkaloids: analyzing the poses of complexes with the acetylcholine binding protein in relation to structure and binding energetics."
Talley T.T., Harel M., Yamauchi G.J., Radic Z., Hansen S., Huxford T., Taylor P.W.
Submitted (OCT-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 18-236.
[13]"The Curare Alkaloids: Analyzing the Poses of Complexes with the Acetylcholine Binding Protein in Relation to Structure and Binding Energies."
Talley T.T., Harel M., Yamauchi J.G., Radic Z., Hansen S., Huxford T., Taylor P.W.
Submitted (NOV-2010) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS) OF 18-236 IN COMPLEX WITH MAGNESIUM.
[14]"Acetylcholine binding protein (AChBP) as template for hierarchical in silico screening procedures to identify structurally novel ligands for the nicotinic receptors."
Akdemir A., Rucktooa P., Jongejan A., Elk R.v., Bertrand S., Sixma T.K., Bertrand D., Smit A.B., Leurs R., de Graaf C., de Esch I.J.
Bioorg. Med. Chem. 19:6107-6119(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS).
[15]"Fragment growing induces conformational changes in acetylcholine-binding protein: a structural and thermodynamic analysis."
Edink E., Rucktooa P., Retra K., Akdemir A., Nahar T., Zuiderveld O., van Elk R., Janssen E., van Nierop P., van Muijlwijk-Koezen J., Smit A.B., Sixma T.K., Leurs R., de Esch I.J.
J. Am. Chem. Soc. 133:5363-5371(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 20-236 IN COMPLEX WITH CHLORIDE AND SULFATE.
[16]"Crystal structures of a cysteine-modified mutant in loop D of acetylcholine-binding protein."
Brams M., Gay E.A., Saez J.C., Guskov A., van Elk R., van der Schors R.C., Peigneur S., Tytgat J., Strelkov S.V., Smit A.B., Yakel J.L., Ulens C.
J. Biol. Chem. 286:4420-4428(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 20-236 IN COMPLEX WITH PHOSPHATE.
[17]"Creating an ?7 nicotinic acetylcholine recognition domain from the acetylcholine-binding protein: crystallographic and ligand selectivity analyses."
Nemecz A., Taylor P.
J. Biol. Chem. 286:42555-42565(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.32 ANGSTROMS) OF 18-236, GLYCOSYLATION AT ASN-91.
[18]"A structural and mutagenic blueprint for molecular recognition of strychnine and d-tubocurarine by different cys-loop receptors."
Brams M., Pandya A., Kuzmin D., van Elk R., Krijnen L., Yakel J.L., Tsetlin V., Smit A.B., Ulens C.
PLoS Biol. 9:e1001034-e1001034(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS) OF 20-236.
[19]"Generation of candidate ligands for nicotinic acetylcholine receptors via in situ click chemistry with a soluble acetylcholine binding protein template."
Grimster N.P., Stump B., Fotsing J.R., Weide T., Talley T.T., Yamauchi J.G., Nemecz A., Kim C., Ho K.Y., Sharpless K.B., Taylor P., Fokin V.V.
J. Am. Chem. Soc. 134:6732-6740(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 18-236, GLYCOSYLATION AT ASN-91.
[20]"Structural characterization of binding mode of smoking cessation drugs to nicotinic acetylcholine receptors through study of ligand complexes with acetylcholine-binding protein."
Rucktooa P., Haseler C.A., van Elk R., Smit A.B., Gallagher T., Sixma T.K.
J. Biol. Chem. 287:23283-23293(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.88 ANGSTROMS) OF 20-236.
[21]"Pairwise interaction of alpha-conotoxin BuIA Pro6 with the beta subunit of nicotinic acetylcholine receptor."
Filchakova O.M., Talley T.T., Reger A.S., Kim C., Ho K., Han K., Taylor P., McIntosh J.M.
Submitted (MAY-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.49 ANGSTROMS) OF 18-236.
[22]"X-ray structure of Acetylcholine binding protein from Aplysia californica in presence of 3-((S)-azetidin-2-ylmethoxy)-5-((1S,2R)-2-(2-methoxyethyl)cyclopropyl)pyridine."
Mukhopadhyay S., Mesecar A.D.
Submitted (JUN-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 18-225.
[23]"Structural basis of ligand recognition in 5-HT3 receptors."
Kesters D., Thompson A.J., Brams M., van Elk R., Spurny R., Geitmann M., Villalgordo J.M., Guskov A., Danielson U.H., Lummis S.C., Smit A.B., Ulens C.
EMBO Rep. 14:49-56(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) OF 20-231 IN COMPLEX WITH PHOSPHATE, GLYCOSYLATION AT ASN-91.
[24]"Assembly of a ?-? stack of ligands in the binding site of an acetylcholine-binding protein."
Stornaiuolo M., De Kloe G.E., Rucktooa P., Fish A., van Elk R., Edink E.S., Bertrand D., Smit A.B., de Esch I.J., Sixma T.K.
Nat. Commun. 4:1875-1875(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF322877 mRNA. Translation: AAL37251.1.
RefSeqNP_001191488.1. NM_001204559.1.
UniGeneAcl.89.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2BR7X-ray3.00A/B/C/D/E20-236[»]
2BR8X-ray2.40A/B/C/D/E20-236[»]
2BYNX-ray2.02A/B/C/D/E18-236[»]
2BYPX-ray2.07A/B/C/D/E18-225[»]
2BYQX-ray3.40A/B/C/D/E18-236[»]
2BYRX-ray2.45A/B/C/D/E/F/G/H/I/J18-236[»]
2BYSX-ray2.05A/B/C/D/E/F/G/H/I/J18-236[»]
2C9TX-ray2.25A/B/C/D/E/F/G/H/I/J20-236[»]
2PGZX-ray1.76A/B/C/D/E18-236[»]
2PH9X-ray2.88A/B/C/D/E18-236[»]
2UZ6X-ray2.40A/B/C/D/E/F/G/H/I/J20-236[»]
2W8FX-ray2.70A/B/C/D/E/F/G/H/I/J20-236[»]
2W8GX-ray2.60A/B/C/D/E20-236[»]
2WN9X-ray1.75A/B/C/D/E18-236[»]
2WNCX-ray2.20A/B/C/D/E18-236[»]
2WNJX-ray1.80A/B/C/D/E18-236[»]
2WNLX-ray2.70A/B/C/D/E/F/G/H/I/J18-236[»]
2WZYX-ray2.51A/B/C/D/E/F/G/H/I/J18-236[»]
2X00X-ray2.40A/B/C/D/E18-236[»]
2XNTX-ray3.21A/B/C/D/E/F/G/H/I/J1-236[»]
2XNUX-ray2.55A/B/C/D/E1-236[»]
2XNVX-ray2.44A/B/C/D/E/F/G/H/I/J1-236[»]
2XYSX-ray1.91A/B/C/D/E20-236[»]
2XYTX-ray2.05A/B/C/D/E/F/G/H/I/J20-236[»]
2XZ5X-ray2.80A/B/C/D/E20-236[»]
2XZ6X-ray3.14A/B/C/D/E/F/G/H/I/J20-236[»]
2Y54X-ray3.65A/B/C/D/E20-236[»]
2Y56X-ray3.59A/B/C/D/E20-236[»]
2Y57X-ray3.30A/B/C/D/E20-236[»]
2Y58X-ray3.25A/B/C/D/E20-236[»]
2Y7YX-ray1.90A/B/C/D/E20-236[»]
2YMDX-ray1.96A/B/C/D/E/F/G/H/I/J20-231[»]
2YMEX-ray2.40A/B/C/D/E/F/G/H/I/J20-224[»]
3C79X-ray2.48A/B/C/D/E18-236[»]
3C84X-ray1.94A/B/C/D/E18-236[»]
3GUAX-ray3.10A/B/C/D/E/F/G/H/I/J18-225[»]
3PEOX-ray2.10A/B/C/D/E/F/G/H/I/J18-236[»]
3PMZX-ray2.44A/B/C/D/E/F/G/H/I/J18-236[»]
3SH1X-ray2.90A/B/C/D/E/F/G/H/I/J18-236[»]
3SIOX-ray2.32A/B/C/D/E/F/G/H/I/J18-236[»]
3T4MX-ray3.00A/B/C/D/E/F/G/H/I/J18-236[»]
4AFTX-ray3.20A/B/C/D/E20-236[»]
4BFQX-ray2.40A/B/C/D/E20-236[»]
4BQTX-ray2.88A/B/C/D/E20-236[»]
4DBMX-ray2.30A/B/C/D/E18-236[»]
4EZ1X-ray2.49A/B/C/D/E18-236[»]
4FRRX-ray2.20A/B/C/D/E/F/G/H/I/J18-225[»]
ProteinModelPortalQ8WSF8.
SMRQ8WSF8. Positions 18-226.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-46103N.
MINTMINT-7300724.

Chemistry

ChEMBLCHEMBL1944497.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100533247.

Family and domain databases

Gene3D2.70.170.10. 1 hit.
InterProIPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
[Graphical view]
SUPFAMSSF63712. SSF63712. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ8WSF8.

Entry information

Entry nameQ8WSF8_APLCA
AccessionPrimary (citable) accession number: Q8WSF8
Entry history
Integrated into UniProtKB/TrEMBL: March 1, 2002
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)