Soluble acetylcholine receptor - Aplysia californica (California sea hare)

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Q8WSF8 (Q8WSF8_APLCA) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 57. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names	Submitted name: Soluble acetylcholine receptor EMBL AAL37251.1
Organism	Aplysia californica (California sea hare) EMBL AAL37251.1
Taxonomic identifier	6500 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Heterobranchia › Euthyneura › Euopisthobranchia › Aplysiomorpha › Aplysioidea › Aplysiidae › Aplysia

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Receptor EMBL AAL37251.1
Technical term	3D-structure PDB 2PGZ PDB 2BYN PDB 2BYP PDB 2WN9 PDB 2WNJ PDB 2WNL PDB 2W8F PDB 2W8G PDB 2PH9 PDB 2BR7 PDB 2BR8 PDB 3C84 PDB 3C79 PDB 2C9T PDB 2WZY PDB 2X00 PDB 3GUA PDB 2BYS PDB 2BYR PDB 2BYQ PDB 2UZ6 PDB 2WNC PDB 2XZ6 PDB 2XZ5 PDB 2XNT PDB 2Y7Y PDB 2XNU PDB 2XYT PDB 2XYS PDB 2XNV PDB 2Y54 PDB 2Y56 PDB 2Y57 PDB 2Y58 PDB 3PEO PDB 3PMZ PDB 3SH1 PDB 3SIO PDB 3T4M PDB 4AFO PDB 4AFT PDB 4DBM PDB 2YMD PDB 2YME
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	extracellular ligand-gated ion channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Binding site

110

Cocaine

Binding site

164

Cocaine

Binding site

205

Cocaine

Binding site

212

Cocaine

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) PDB 2PGZ PDB 2BYN PDB 2BYP PDB 2WN9 PDB 2WNJ PDB 2WNL

Sequences

Sequence

Length

Mass (Da)

Tools

Q8WSF8 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 6D0DE487C46707BE
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FASTA

236

26,568

        10         20         30         40         50         60 
MLVSVYLALL VACVGQAHSQ ANLMRLKSDL FNRSPMYPGP TKDDPLTVTL GFTLQDIVKA 

        70         80         90        100        110        120 
DSSTNEVDLV YYEQQRWKLN SLMWDPNEYG NITDFRTSAA DIWTPDITAY SSTRPVQVLS 

       130        140        150        160        170        180 
PQIAVVTHDG SVMFIPAQRL SFMCDPTGVD SEEGATCAVK FGSWVYSGFE IDLKTDTDQV 

       190        200        210        220        230 
DLSSYYASSK YEILSATQTR QVQHYSCCPE PYIDVNLVVK FRERRAGNGF FRNLFD

« Hide

References

[1]	"Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive binding interfaces and conformations." Hansen S.B., Sulzenbacher G., Huxford T., Marchot P., Taylor P., Bourne Y. EMBO J. 24:3635-3646(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 18-236, GLYCOSYLATION AT ASN-91.
[2]	"Crystal structure of nicotinic acetylcholine receptor homolog AChBP in complex with an alpha-conotoxin PnIA variant." Celie P.H., Kasheverov I.E., Mordvintsev D.Y., Hogg R.C., van Nierop P., van Elk R., van Rossum-Fikkert S.E., Zhmak M.N., Bertrand D., Tsetlin V., Sixma T.K., Smit A.B. Nat. Struct. Mol. Biol. 12:582-588(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236.
[3]	"Neuronal transcriptome of Aplysia: neuronal compartments and circuitry." Moroz L.L., Edwards J.R., Puthanveettil S.V., Kohn A.B., Ha T., Heyland A., Knudsen B., Sahni A., Yu F., Liu L., Jezzini S., Lovell P., Iannucculli W., Chen M., Nguyen T., Sheng H., Shaw R., Kalachikov S. Kandel E.R. Cell 127:1453-1467(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Structural determinants of selective alpha-conotoxin binding to a nicotinic acetylcholine receptor homolog AChBP." Ulens C., Hogg R.C., Celie P.H., Bertrand D., Tsetlin V., Smit A.B., Sixma T.K. Proc. Natl. Acad. Sci. U.S.A. 103:3615-3620(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 20-236.
[5]	"AChBP-targeted alpha-conotoxin correlates distinct binding orientations with nAChR subtype selectivity." Dutertre S., Ulens C., Buttner R., Fish A., van Elk R., Kendel Y., Hopping G., Alewood P.F., Schroeder C., Nicke A., Smit A.B., Sixma T.K., Lewis R.J. EMBO J. 26:3858-3867(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 20-236.
[6]	"Galanthamine and non-competitive inhibitor binding to ACh-binding protein: evidence for a binding site on non-alpha-subunit interfaces of heteromeric neuronal nicotinic receptors." Hansen S.B., Taylor P. J. Mol. Biol. 369:895-901(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 18-236 IN COMPLEX WITH COCAINE, GLYCOSYLATION AT ASN-91.
[7]	"An ion selectivity filter in the extracellular domain of Cys-loop receptors reveals determinants for ion conductance." Hansen S.B., Wang H.L., Taylor P., Sine S.M. J. Biol. Chem. 283:36066-36070(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 18-225.
[8]	"Atomic interactions of neonicotinoid agonists with AChBP: molecular recognition of the distinctive electronegative pharmacophore." Talley T.T., Harel M., Hibbs R.E., Radic Z., Tomizawa M., Casida J.E., Taylor P. Proc. Natl. Acad. Sci. U.S.A. 105:7606-7611(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.94 ANGSTROMS) OF 18-236.
[9]	"Structural determinants for interaction of partial agonists with acetylcholine binding protein and neuronal alpha7 nicotinic acetylcholine receptor." Hibbs R.E., Sulzenbacher G., Shi J., Talley T.T., Conrod S., Kem W.R., Taylor P., Marchot P., Bourne Y. EMBO J. 28:3040-3051(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 18-236, GLYCOSYLATION AT ASN-91.
[10]	"Use of acetylcholine binding protein in the search for novel alpha7 nicotinic receptor ligands. In silico docking, pharmacological screening, and X-ray analysis." Ulens C., Akdemir A., Jongejan A., van Elk R., Bertrand S., Perrakis A., Leurs R., Smit A.B., Sixma T.K., Bertrand D., de Esch I.J. J. Med. Chem. 52:2372-2383(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 20-236.
[11]	"Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism." Bourne Y., Radic Z., Araoz R., Talley T.T., Benoit E., Servent D., Taylor P., Molgo J., Marchot P. Proc. Natl. Acad. Sci. U.S.A. 107:6076-6081(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) OF 18-236.
[12]	"The curare alkaloids: analyzing the poses of complexes with the acetylcholine binding protein in relation to structure and binding energetics." Talley T.T., Harel M., Yamauchi G.J., Radic Z., Hansen S., Huxford T., Taylor P.W. Submitted (OCT-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 18-236.
[13]	"The Curare Alkaloids: Analyzing the Poses of Complexes with the Acetylcholine Binding Protein in Relation to Structure and Binding Energies." Talley T.T., Harel M., Yamauchi J.G., Radic Z., Hansen S., Huxford T., Taylor P.W. Submitted (NOV-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS) OF 18-236.
[14]	"Acetylcholine binding protein (AChBP) as template for hierarchical in silico screening procedures to identify structurally novel ligands for the nicotinic receptors." Akdemir A., Rucktooa P., Jongejan A., Elk R.v., Bertrand S., Sixma T.K., Bertrand D., Smit A.B., Leurs R., de Graaf C., de Esch I.J. Bioorg. Med. Chem. 19:6107-6119(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS).
[15]	"Fragment growing induces conformational changes in acetylcholine-binding protein: a structural and thermodynamic analysis." Edink E., Rucktooa P., Retra K., Akdemir A., Nahar T., Zuiderveld O., van Elk R., Janssen E., van Nierop P., van Muijlwijk-Koezen J., Smit A.B., Sixma T.K., Leurs R., de Esch I.J. J. Am. Chem. Soc. 133:5363-5371(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF 20-236.
[16]	"Crystal structures of a cysteine-modified mutant in loop D of acetylcholine-binding protein." Brams M., Gay E.A., Saez J.C., Guskov A., van Elk R., van der Schors R.C., Peigneur S., Tytgat J., Strelkov S.V., Smit A.B., Yakel J.L., Ulens C. J. Biol. Chem. 286:4420-4428(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 20-236.
[17]	"A structural and mutagenic blueprint for molecular recognition of strychnine and d-tubocurarine by different cys-loop receptors." Brams M., Pandya A., Kuzmin D., van Elk R., Krijnen L., Yakel J.L., Tsetlin V., Smit A.B., Ulens C. PLoS Biol. 9:e1001034-e1001034(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS) OF 20-236.
[18]	"Generation of candidate ligands for nicotinic acetylcholine receptors via in situ click chemistry with a soluble acetylcholine binding protein template." Grimster N.P., Stump B., Fotsing J.R., Weide T., Talley T.T., Yamauchi J.G., Nemecz A., Kim C., Ho K.Y., Sharpless K.B., Taylor P., Fokin V.V. J. Am. Chem. Soc. 134:6732-6740(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 18-236.
[19]	"Structural characterization of binding mode of smoking cessation drugs to nicotinic acetylcholine receptors through study of ligand complexes with acetylcholine-binding protein." Rucktooa P., Haseler C.A., van Elk R., Smit A.B., Gallagher T., Sixma T.K. J. Biol. Chem. 287:23283-23293(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.88 ANGSTROMS) OF 20-236.
[20]	"Structural basis of ligand recognition in 5-HT3 receptors." Kesters D., Thompson A.J., Brams M., van Elk R., Spurny R., Geitmann M., Villalgordo J.M., Guskov A., Danielson U.H., Lummis S.C., Smit A.B., Ulens C. EMBO Rep. 14:49-56(2013) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) OF 20-231.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF322877 mRNA. Translation: AAL37251.1.

RefSeq

NP_001191488.1. NM_001204559.1.

UniGene

Acl.89.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BR7	X-ray	3.00	A/B/C/D/E	20-236	[»]
2BR8	X-ray	2.40	A/B/C/D/E	20-236	[»]
2BYN	X-ray	2.02	A/B/C/D/E	18-236	[»]
2BYP	X-ray	2.07	A/B/C/D/E	18-225	[»]
2BYQ	X-ray	3.40	A/B/C/D/E	18-236	[»]
2BYR	X-ray	2.45	A/B/C/D/E/F/G/H/I/J	18-236	[»]
2BYS	X-ray	2.05	A/B/C/D/E/F/G/H/I/J	18-236	[»]
2C9T	X-ray	2.25	A/B/C/D/E/F/G/H/I/J	20-236	[»]
2PGZ	X-ray	1.76	A/B/C/D/E	18-236	[»]
2PH9	X-ray	2.88	A/B/C/D/E	18-236	[»]
2UZ6	X-ray	2.40	A/B/C/D/E/F/G/H/I/J	20-236	[»]
2W8F	X-ray	2.70	A/B/C/D/E/F/G/H/I/J	20-236	[»]
2W8G	X-ray	2.60	A/B/C/D/E	20-236	[»]
2WN9	X-ray	1.75	A/B/C/D/E	18-236	[»]
2WNC	X-ray	2.20	A/B/C/D/E	18-236	[»]
2WNJ	X-ray	1.80	A/B/C/D/E	18-236	[»]
2WNL	X-ray	2.70	A/B/C/D/E/F/G/H/I/J	18-236	[»]
2WZY	X-ray	2.51	A/B/C/D/E/F/G/H/I/J	18-236	[»]
2X00	X-ray	2.40	A/B/C/D/E	18-236	[»]
2XNT	X-ray	3.21	A/B/C/D/E/F/G/H/I/J	1-236	[»]
2XNU	X-ray	2.55	A/B/C/D/E	1-236	[»]
2XNV	X-ray	2.44	A/B/C/D/E/F/G/H/I/J	1-236	[»]
2XYS	X-ray	1.91	A/B/C/D/E	20-236	[»]
2XYT	X-ray	2.05	A/B/C/D/E/F/G/H/I/J	20-236	[»]
2XZ5	X-ray	2.80	A/B/C/D/E	20-236	[»]
2XZ6	X-ray	3.14	A/B/C/D/E/F/G/H/I/J	20-236	[»]
2Y54	X-ray	3.65	A/B/C/D/E	20-236	[»]
2Y56	X-ray	3.59	A/B/C/D/E	20-236	[»]
2Y57	X-ray	3.30	A/B/C/D/E	20-236	[»]
2Y58	X-ray	3.25	A/B/C/D/E	20-236	[»]
2Y7Y	X-ray	1.90	A/B/C/D/E	20-236	[»]
2YMD	X-ray	1.96	A/B/C/D/E/F/G/H/I/J	20-231	[»]
2YME	X-ray	2.40	A/B/C/D/E/F/G/H/I/J	20-224	[»]
3C79	X-ray	2.48	A/B/C/D/E	18-236	[»]
3C84	X-ray	1.94	A/B/C/D/E	18-236	[»]
3GUA	X-ray	3.10	A/B/C/D/E/F/G/H/I/J	18-225	[»]
3PEO	X-ray	2.10	A/B/C/D/E/F/G/H/I/J	18-236	[»]
3PMZ	X-ray	2.44	A/B/C/D/E/F/G/H/I/J	18-236	[»]
3SH1	X-ray	2.90	A/B/C/D/E/F/G/H/I/J	18-236	[»]
3SIO	X-ray	2.32	A/B/C/D/E/F/G/H/I/J	18-236	[»]
3T4M	X-ray	3.00	A/B/C/D/E/F/G/H/I/J	18-236	[»]
4AFO	X-ray	2.88	A/B/C/D/E	20-236	[»]
4AFT	X-ray	3.20	A/B/C/D/E	20-236	[»]
4DBM	X-ray	2.30	A/B/C/D/E	18-236	[»]

ProteinModelPortal

Q8WSF8.

SMR

Q8WSF8. Positions 18-226.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46103N.

MINT

MINT-7300724.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

100533247.

Family and domain databases

Gene3D

2.70.170.10. 1 hit.

InterPro

IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
[Graphical view]

PANTHER

PTHR18945. PTHR18945. 1 hit.

Pfam

PF02931. Neur_chan_LBD. 1 hit.
[Graphical view]

SUPFAM

SSF63712. Neur_chan_LBD. 1 hit.

ProtoNet

Search...

Other

ChEMBL

CHEMBL1944497.

EvolutionaryTrace

Q8WSF8.

Entry information

Entry name

Q8WSF8_APLCA

Accession

Primary (citable) accession number: Q8WSF8

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2002
Last sequence update:	March 1, 2002
Last modified:	May 29, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Preferred order of sections

Names and origin

Protein attributes

Ontologies

Sequence annotation (Features)

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Family and domain databases

Other

Entry information