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Q8WSF3 (FDL_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable beta-hexosaminidase fdl

EC=3.2.1.52
Alternative name(s):
Protein fused lobes
Gene names
Name:fdl
ORF Names:CG8824
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length660 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in brain restructurization via hormonal control during metamorphosis. Implicated in N-glycan processing. Ref.1 Ref.5

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. UniProtKB P49010

Tissue specificity

In third instar larval and early pupal brains, expressed in cells sending projections across the interhemispheric junction. In adult brain, expressed in mushroom body, ellipsoid body and pars intercerebralis. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 20 family.

Sequence caution

The sequence AAL28585.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform C (identifier: Q8WSF3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q8WSF3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MRYVYESLRYLYKM
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3636 Potential
Chain37 – 660624Probable beta-hexosaminidase fdl
PRO_0000012017

Amino acid modifications

Glycosylation2101N-linked (GlcNAc...) Potential
Glycosylation4121N-linked (GlcNAc...) Potential
Glycosylation4521N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence11M → MRYVYESLRYLYKM in isoform B.
VSP_011911

Experimental info

Sequence conflict1541V → G in AAM29423. Ref.4
Sequence conflict4951C → R in AAM29423. Ref.4
Sequence conflict5511P → Q in AAM29423. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform C [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 0F14B47C9A55CD74

FASTA66075,486
        10         20         30         40         50         60 
MSLAVSLRRA LLVLLTGAIF ILTVLYWNQG VTKAQAYNEA LERPHSHHDA SGFPIPVEKS 

        70         80         90        100        110        120 
WTYKCENDRC MRVGHHGKSA KRVSFISCSM TCGDVNIWPH PTQKFLLSSQ THSFSVEDVQ 

       130        140        150        160        170        180 
LHVDTAHREV RKQLQLAFDW FLKDLRLIQR LDYVGSSSEP TVSESSSKSR HHADLEPAAT 

       190        200        210        220        230        240 
LFGATFGVKK AGDLTSVQVK ISVLKSGDLN FSLDNDETYQ LSTQTEGHRL QVEIIANSYF 

       250        260        270        280        290        300 
GARHGLSTLQ QLIWFDDEDH LLHTYANSKV KDAPKFRYRG LMLDTSRHFF SVESIKRTIV 

       310        320        330        340        350        360 
GMGLAKMNRF HWHLTDAQSF PYISRYYPEL AVHGAYSESE TYSEQDVREV AEFAKIYGVQ 

       370        380        390        400        410        420 
VIPEIDAPAH AGNGWDWGPK RGMGELAMCI NQQPWSFYCG EPPCGQLNPK NNYTYLILQR 

       430        440        450        460        470        480 
IYEELLQHTG PTDFFHLGGD EVNLDCWAQY FNDTDLRGLW CDFMLQAMAR LKLANNGVAP 

       490        500        510        520        530        540 
KHVAVWSSAL TNTKCLPNSQ FTVQVWGGST WQENYDLLDN GYNVIFSHVD AWYLDCGFGS 

       550        560        570        580        590        600 
WRATGDAACA PYRTWQNVYK HRPWERMRLD KKRKKQVLGG EVCMWTEQVD ENQLDNRLWP 

       610        620        630        640        650        660 
RTAALAERLW TDPSDDHDMD IVPPDVFRRI SLFRNRLVEL GIRAEALFPK YCAQNPGECI 

« Hide

Isoform B [UniParc].

Checksum: 7A73275BB491AE7D
Show »

FASTA67377,252

References

« Hide 'large scale' references
[1]"Central brain postembryonic development in Drosophila: implication of genes expressed at the interhemispheric junction."
Boquet I., Hitier R., Dumas M., Chaminade M., Preat T.
J. Neurobiol. 42:33-48(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM C), FUNCTION, TISSUE SPECIFICITY.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
Strain: Berkeley.
Tissue: Embryo and Head.
[5]Leonard R., Altmann F.
Submitted (APR-2003) to UniProtKB
Cited for: FUNCTION IN N-GLYCAN PROCESSING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF323977 mRNA. Translation: AAL55992.1.
AE013599 Genomic DNA. Translation: AAM68691.2.
AE013599 Genomic DNA. Translation: AAM68692.1.
AY113418 mRNA. Translation: AAM29423.1.
AY061037 mRNA. Translation: AAL28585.1. Different initiation.
RefSeqNP_725178.2. NM_165908.1. [Q8WSF3-2]
NP_725179.1. NM_165909.2. [Q8WSF3-1]
UniGeneDm.3735.

3D structure databases

ProteinModelPortalQ8WSF3.
SMRQ8WSF3. Positions 177-652.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid75345. 4 interactions.
DIPDIP-21467N.
MINTMINT-1665363.
STRING7227.FBpp0087058.

Protein family/group databases

CAZyGH20. Glycoside Hydrolase Family 20.

Proteomic databases

PaxDbQ8WSF3.
PRIDEQ8WSF3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0087946; FBpp0087057; FBgn0045063. [Q8WSF3-1]
GeneID250735.
KEGGdme:Dmel_CG8824.

Organism-specific databases

CTD250735.
FlyBaseFBgn0045063. fdl.

Phylogenomic databases

eggNOGCOG3525.
GeneTreeENSGT00390000008107.
InParanoidQ8WSF3.
KOK12373.
OMAVYKHRPW.
OrthoDBEOG79W94H.
PhylomeDBQ8WSF3.

Gene expression databases

BgeeQ8WSF3.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProIPR025705. Beta_hexosaminidase_sua/sub.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR015883. Glyco_hydro_20_cat-core.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR029019. HEX_eukaryotic_N.
[Graphical view]
PfamPF00728. Glyco_hydro_20. 1 hit.
PF14845. Glycohydro_20b2. 1 hit.
[Graphical view]
PIRSFPIRSF001093. B-hxosamndse_ab_euk_. 1 hit.
PRINTSPR00738. GLHYDRLASE20.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 2 hits.
ProtoNetSearch...

Other

GenomeRNAi250735.
NextBio843498.

Entry information

Entry nameFDL_DROME
AccessionPrimary (citable) accession number: Q8WSF3
Secondary accession number(s): Q8MZ16, Q95RY8, Q9V6C8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase