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Protein

GDP-fucose protein O-fucosyltransferase 2

Gene

pad-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombosporin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13 (By similarity).By similarity1 Publication

Catalytic activityi

Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei52 – 521Proton donor/acceptorBy similarity
Binding sitei290 – 2901SubstrateBy similarity
Sitei391 – 3911Essential for catalytic activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Fucose metabolism

Enzyme and pathway databases

ReactomeiR-CEL-5173214. O-glycosylation of TSR domain-containing proteins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT68. Glycosyltransferase Family 68.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-fucose protein O-fucosyltransferase 2 (EC:2.4.1.221)
Alternative name(s):
Patterning defective protein 2
Peptide-O-fucosyltransferase 2
Short name:
O-FucT-2
Gene namesi
Name:pad-2
ORF Names:K10G9.3
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiK10G9.3a; CE31715; WBGene00010757; pad-2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 424404GDP-fucose protein O-fucosyltransferase 2PRO_0000012157Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi154 ↔ 187By similarity
Glycosylationi205 – 2051N-linked (GlcNAc...)1 Publication
Disulfide bondi407 ↔ 414By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8WR51.
PaxDbiQ8WR51.

Expressioni

Tissue specificityi

Expressed in the anterior part of embryos, in the hypodermal and neuronal cells of the head. Expressed at different levels in a variety of cell types after hatching, including neuronal, hypodermal, muscle, intestinal, and somatic gonadal cells. Expressed in the nerve ring around the pharynx, in dorsal and ventral nerve cords, intestine, and a variety of hypodermal cells of L1-L3 larvae. Expressed in gonadal sheath cells, spermatheca, and tissues surrounding the vulva of adult hermaphrodites, and in the body wall muscle and hypodermal cells of adults of both sexes.1 Publication

Developmental stagei

Expressed starts at morphogenetic embryonic stages and continues throughout development.1 Publication

Gene expression databases

ExpressionAtlasiQ8WR51. baseline.

Interactioni

Protein-protein interaction databases

STRINGi6239.K10G9.3b.

Structurei

Secondary structure

1
424
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi41 – 466Combined sources
Helixi54 – 7320Combined sources
Beta strandi77 – 826Combined sources
Helixi100 – 1023Combined sources
Helixi106 – 1127Combined sources
Beta strandi115 – 1173Combined sources
Helixi118 – 1236Combined sources
Beta strandi130 – 1323Combined sources
Beta strandi148 – 1514Combined sources
Helixi158 – 1614Combined sources
Beta strandi163 – 1664Combined sources
Beta strandi169 – 1713Combined sources
Helixi174 – 1763Combined sources
Beta strandi188 – 1914Combined sources
Helixi194 – 2029Combined sources
Helixi204 – 2063Combined sources
Beta strandi210 – 2167Combined sources
Helixi217 – 2193Combined sources
Helixi228 – 2358Combined sources
Helixi241 – 25515Combined sources
Helixi259 – 2624Combined sources
Helixi270 – 2723Combined sources
Beta strandi282 – 2898Combined sources
Helixi292 – 2965Combined sources
Helixi299 – 3013Combined sources
Helixi305 – 31915Combined sources
Beta strandi323 – 3286Combined sources
Helixi332 – 3409Combined sources
Beta strandi347 – 3493Combined sources
Helixi359 – 37113Combined sources
Beta strandi373 – 3786Combined sources
Helixi383 – 39513Combined sources
Helixi399 – 4013Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5FOEX-ray1.98A/B1-424[»]
ProteinModelPortaliQ8WR51.
SMRiQ8WR51. Positions 39-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 544Substrate bindingBy similarity
Regioni383 – 3842Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 68 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFPR. Eukaryota.
ENOG410Y78N. LUCA.
GeneTreeiENSGT00390000007989.
HOGENOMiHOG000015874.
InParanoidiQ8WR51.
PhylomeDBiQ8WR51.

Family and domain databases

InterProiIPR019378. GDP-Fuc_O-FucTrfase.
[Graphical view]
PfamiPF10250. O-FucT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8WR51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFFPIQLLV LFFAEKIAFA ENSDQTVSRV DSNRYSVAAE KKFLLYDVNF
60 70 80 90 100
GEGFNLRRDV YMRVANTVRS LRDSGENYIL VLPPWGRLHH WKRMEVALSW
110 120 130 140 150
RLFFDLESLN RFIPVIEFED FLDENRPIDQ VIYLQHYAEG WGTEYVRKFE
160 170 180 190 200
KRSCLPPAES HYKQVEEFKW KGWFYSYEDV YSRNFQCVSI QGDSGTLKDL
210 220 230 240 250
LKHSNFSEST SIMVDRAETI LHEHYGEVDY WKARRSMRYS NDLVDVADAF
260 270 280 290 300
RKKYLDSDDK RDKTKLVDDW TKEKPRRTAI GGPYLGIHWR RRDFLYARRA
310 320 330 340 350
QLPTIPGTAK ILQDLCKKLD LQKIYLATDA PDQEVDELKA LLNGELEVYR
360 370 380 390 400
FTDTQKLNDG QIAIIDQYLC AHAAYFIGSY ESTFTFRIQE DREIIGFPIS
410 420
TTFNRLCPDT EPTCEQPAKW KIVY
Length:424
Mass (Da):50,106
Last modified:March 1, 2002 - v1
Checksum:i2A35F5FC04EF70B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF455271 mRNA. Translation: AAL65193.1.
Z36282 Genomic DNA. Translation: CAD45602.1.
RefSeqiNP_001255070.1. NM_001268141.1.
UniGeneiCel.9168.

Genome annotation databases

EnsemblMetazoaiK10G9.3a; K10G9.3a; WBGene00010757.
GeneIDi259529.
UCSCiK10G9.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF455271 mRNA. Translation: AAL65193.1.
Z36282 Genomic DNA. Translation: CAD45602.1.
RefSeqiNP_001255070.1. NM_001268141.1.
UniGeneiCel.9168.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5FOEX-ray1.98A/B1-424[»]
ProteinModelPortaliQ8WR51.
SMRiQ8WR51. Positions 39-424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.K10G9.3b.

Protein family/group databases

CAZyiGT68. Glycosyltransferase Family 68.

Proteomic databases

EPDiQ8WR51.
PaxDbiQ8WR51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK10G9.3a; K10G9.3a; WBGene00010757.
GeneIDi259529.
UCSCiK10G9.3. c. elegans.

Organism-specific databases

CTDi259529.
WormBaseiK10G9.3a; CE31715; WBGene00010757; pad-2.

Phylogenomic databases

eggNOGiENOG410IFPR. Eukaryota.
ENOG410Y78N. LUCA.
GeneTreeiENSGT00390000007989.
HOGENOMiHOG000015874.
InParanoidiQ8WR51.
PhylomeDBiQ8WR51.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-CEL-5173214. O-glycosylation of TSR domain-containing proteins.

Miscellaneous databases

NextBioi952052.
PROiQ8WR51.

Gene expression databases

ExpressionAtlasiQ8WR51. baseline.

Family and domain databases

InterProiIPR019378. GDP-Fuc_O-FucTrfase.
[Graphical view]
PfamiPF10250. O-FucT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Caenorhabditis elegans ortholog of C21orf80, a potential new protein O-fucosyltransferase, is required for normal development."
    Menzel O., Vellai T., Takacs-Vellai K., Reymond A., Mueller F., Antonarakis S.E., Guipponi M.
    Genomics 84:320-330(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-205, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiOFUT2_CAEEL
AccessioniPrimary (citable) accession number: Q8WR51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2002
Last modified: April 13, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.