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Q8WR51

- OFUT2_CAEEL

UniProt

Q8WR51 - OFUT2_CAEEL

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Protein

GDP-fucose protein O-fucosyltransferase 2

Gene
pad-2, K10G9.3
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombosporin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13 By similarity.1 Publication

Catalytic activityi

Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei52 – 521Proton donor/acceptor By similarity
Binding sitei290 – 2901Substrate By similarity
Sitei391 – 3911Essential for catalytic activity By similarity

GO - Molecular functioni

  1. peptide-O-fucosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. fucose metabolic process Source: UniProtKB-KW
  2. protein O-linked fucosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Fucose metabolism

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT68. Glycosyltransferase Family 68.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-fucose protein O-fucosyltransferase 2 (EC:2.4.1.221)
Alternative name(s):
Patterning defective protein 2
Peptide-O-fucosyltransferase 2
Short name:
O-FucT-2
Gene namesi
Name:pad-2
ORF Names:K10G9.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiK10G9.3a; CE31715; WBGene00010757; pad-2.

Subcellular locationi

Endoplasmic reticulum By similarity. Golgi apparatus
Note: Mainly located in the endoplasmic reticulum.1 Publication

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-SubCell
  2. Golgi apparatus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Chaini21 – 424404GDP-fucose protein O-fucosyltransferase 2PRO_0000012157Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi154 ↔ 187 By similarity
Glycosylationi205 – 2051N-linked (GlcNAc...)1 Publication
Disulfide bondi407 ↔ 414 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8WR51.

Expressioni

Tissue specificityi

Expressed in the anterior part of embryos, in the hypodermal and neuronal cells of the head. Expressed at different levels in a variety of cell types after hatching, including neuronal, hypodermal, muscle, intestinal, and somatic gonadal cells. Expressed in the nerve ring around the pharynx, in dorsal and ventral nerve cords, intestine, and a variety of hypodermal cells of L1-L3 larvae. Expressed in gonadal sheath cells, spermatheca, and tissues surrounding the vulva of adult hermaphrodites, and in the body wall muscle and hypodermal cells of adults of both sexes.1 Publication

Developmental stagei

Expressed starts at morphogenetic embryonic stages and continues throughout development.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi6239.K10G9.3.

Structurei

3D structure databases

ProteinModelPortaliQ8WR51.
SMRiQ8WR51. Positions 39-424.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 544Substrate binding By similarity
Regioni383 – 3842Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG77810.
GeneTreeiENSGT00390000007989.
HOGENOMiHOG000015874.
InParanoidiQ8WR51.
KOiK03691.
PhylomeDBiQ8WR51.

Family and domain databases

InterProiIPR019378. GDP-Fuc_O-FucTrfase.
[Graphical view]
PfamiPF10250. O-FucT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8WR51-1 [UniParc]FASTAAdd to Basket

« Hide

MHFFPIQLLV LFFAEKIAFA ENSDQTVSRV DSNRYSVAAE KKFLLYDVNF    50
GEGFNLRRDV YMRVANTVRS LRDSGENYIL VLPPWGRLHH WKRMEVALSW 100
RLFFDLESLN RFIPVIEFED FLDENRPIDQ VIYLQHYAEG WGTEYVRKFE 150
KRSCLPPAES HYKQVEEFKW KGWFYSYEDV YSRNFQCVSI QGDSGTLKDL 200
LKHSNFSEST SIMVDRAETI LHEHYGEVDY WKARRSMRYS NDLVDVADAF 250
RKKYLDSDDK RDKTKLVDDW TKEKPRRTAI GGPYLGIHWR RRDFLYARRA 300
QLPTIPGTAK ILQDLCKKLD LQKIYLATDA PDQEVDELKA LLNGELEVYR 350
FTDTQKLNDG QIAIIDQYLC AHAAYFIGSY ESTFTFRIQE DREIIGFPIS 400
TTFNRLCPDT EPTCEQPAKW KIVY 424
Length:424
Mass (Da):50,106
Last modified:March 1, 2002 - v1
Checksum:i2A35F5FC04EF70B3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF455271 mRNA. Translation: AAL65193.1.
Z36282 Genomic DNA. Translation: CAD45602.1.
RefSeqiNP_001255070.1. NM_001268141.1.
UniGeneiCel.9168.

Genome annotation databases

EnsemblMetazoaiK10G9.3a; K10G9.3a; WBGene00010757.
GeneIDi259529.
KEGGicel:CELE_K10G9.3.
UCSCiK10G9.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF455271 mRNA. Translation: AAL65193.1 .
Z36282 Genomic DNA. Translation: CAD45602.1 .
RefSeqi NP_001255070.1. NM_001268141.1.
UniGenei Cel.9168.

3D structure databases

ProteinModelPortali Q8WR51.
SMRi Q8WR51. Positions 39-424.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6239.K10G9.3.

Protein family/group databases

CAZyi GT68. Glycosyltransferase Family 68.

Proteomic databases

PaxDbi Q8WR51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai K10G9.3a ; K10G9.3a ; WBGene00010757 .
GeneIDi 259529.
KEGGi cel:CELE_K10G9.3.
UCSCi K10G9.3. c. elegans.

Organism-specific databases

CTDi 259529.
WormBasei K10G9.3a ; CE31715 ; WBGene00010757 ; pad-2.

Phylogenomic databases

eggNOGi NOG77810.
GeneTreei ENSGT00390000007989.
HOGENOMi HOG000015874.
InParanoidi Q8WR51.
KOi K03691.
PhylomeDBi Q8WR51.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 952052.
PROi Q8WR51.

Family and domain databases

InterProi IPR019378. GDP-Fuc_O-FucTrfase.
[Graphical view ]
Pfami PF10250. O-FucT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Caenorhabditis elegans ortholog of C21orf80, a potential new protein O-fucosyltransferase, is required for normal development."
    Menzel O., Vellai T., Takacs-Vellai K., Reymond A., Mueller F., Antonarakis S.E., Guipponi M.
    Genomics 84:320-330(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-205, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiOFUT2_CAEEL
AccessioniPrimary (citable) accession number: Q8WR51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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