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Protein

GDP-fucose protein O-fucosyltransferase 2

Gene

pad-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombosporin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13 (By similarity).By similarity1 Publication

Catalytic activityi

Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei52Proton donor/acceptorBy similarity1
Binding sitei290SubstrateBy similarity1
Sitei391Essential for catalytic activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Fucose metabolism

Enzyme and pathway databases

ReactomeiR-CEL-5173214. O-glycosylation of TSR domain-containing proteins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT68. Glycosyltransferase Family 68.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-fucose protein O-fucosyltransferase 2 (EC:2.4.1.221)
Alternative name(s):
Patterning defective protein 2
Peptide-O-fucosyltransferase 2
Short name:
O-FucT-2
Gene namesi
Name:pad-2
ORF Names:K10G9.3
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiK10G9.3a; CE31715; WBGene00010757; pad-2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000001215721 – 424GDP-fucose protein O-fucosyltransferase 2Add BLAST404

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi154 ↔ 187By similarity
Glycosylationi205N-linked (GlcNAc...)1 Publication1
Disulfide bondi407 ↔ 414By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ8WR51.
PaxDbiQ8WR51.

Expressioni

Tissue specificityi

Expressed in the anterior part of embryos, in the hypodermal and neuronal cells of the head. Expressed at different levels in a variety of cell types after hatching, including neuronal, hypodermal, muscle, intestinal, and somatic gonadal cells. Expressed in the nerve ring around the pharynx, in dorsal and ventral nerve cords, intestine, and a variety of hypodermal cells of L1-L3 larvae. Expressed in gonadal sheath cells, spermatheca, and tissues surrounding the vulva of adult hermaphrodites, and in the body wall muscle and hypodermal cells of adults of both sexes.1 Publication

Developmental stagei

Expressed starts at morphogenetic embryonic stages and continues throughout development.1 Publication

Gene expression databases

BgeeiWBGene00010757.
ExpressionAtlasiQ8WR51. differential.

Interactioni

Protein-protein interaction databases

STRINGi6239.K10G9.3b.

Structurei

Secondary structure

1424
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 46Combined sources6
Helixi54 – 73Combined sources20
Beta strandi77 – 82Combined sources6
Helixi100 – 102Combined sources3
Helixi106 – 112Combined sources7
Beta strandi115 – 117Combined sources3
Helixi118 – 123Combined sources6
Beta strandi130 – 132Combined sources3
Beta strandi148 – 151Combined sources4
Helixi158 – 161Combined sources4
Beta strandi163 – 166Combined sources4
Beta strandi169 – 171Combined sources3
Helixi174 – 176Combined sources3
Beta strandi188 – 191Combined sources4
Helixi194 – 202Combined sources9
Helixi204 – 206Combined sources3
Beta strandi210 – 216Combined sources7
Helixi217 – 219Combined sources3
Helixi228 – 235Combined sources8
Helixi241 – 255Combined sources15
Helixi259 – 262Combined sources4
Helixi270 – 272Combined sources3
Beta strandi282 – 289Combined sources8
Helixi292 – 296Combined sources5
Helixi299 – 301Combined sources3
Helixi305 – 319Combined sources15
Beta strandi323 – 328Combined sources6
Helixi332 – 340Combined sources9
Beta strandi347 – 349Combined sources3
Helixi359 – 371Combined sources13
Beta strandi373 – 378Combined sources6
Helixi383 – 395Combined sources13
Helixi399 – 401Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FOEX-ray1.98A/B1-424[»]
ProteinModelPortaliQ8WR51.
SMRiQ8WR51.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 54Substrate bindingBy similarity4
Regioni383 – 384Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyltransferase 68 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IFPR. Eukaryota.
ENOG410Y78N. LUCA.
GeneTreeiENSGT00390000007989.
HOGENOMiHOG000015874.
InParanoidiQ8WR51.
PhylomeDBiQ8WR51.

Family and domain databases

InterProiIPR019378. GDP-Fuc_O-FucTrfase.
[Graphical view]
PfamiPF10250. O-FucT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8WR51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFFPIQLLV LFFAEKIAFA ENSDQTVSRV DSNRYSVAAE KKFLLYDVNF
60 70 80 90 100
GEGFNLRRDV YMRVANTVRS LRDSGENYIL VLPPWGRLHH WKRMEVALSW
110 120 130 140 150
RLFFDLESLN RFIPVIEFED FLDENRPIDQ VIYLQHYAEG WGTEYVRKFE
160 170 180 190 200
KRSCLPPAES HYKQVEEFKW KGWFYSYEDV YSRNFQCVSI QGDSGTLKDL
210 220 230 240 250
LKHSNFSEST SIMVDRAETI LHEHYGEVDY WKARRSMRYS NDLVDVADAF
260 270 280 290 300
RKKYLDSDDK RDKTKLVDDW TKEKPRRTAI GGPYLGIHWR RRDFLYARRA
310 320 330 340 350
QLPTIPGTAK ILQDLCKKLD LQKIYLATDA PDQEVDELKA LLNGELEVYR
360 370 380 390 400
FTDTQKLNDG QIAIIDQYLC AHAAYFIGSY ESTFTFRIQE DREIIGFPIS
410 420
TTFNRLCPDT EPTCEQPAKW KIVY
Length:424
Mass (Da):50,106
Last modified:March 1, 2002 - v1
Checksum:i2A35F5FC04EF70B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF455271 mRNA. Translation: AAL65193.1.
Z36282 Genomic DNA. Translation: CAD45602.1.
RefSeqiNP_001255070.1. NM_001268141.1.
UniGeneiCel.9168.

Genome annotation databases

EnsemblMetazoaiK10G9.3a; K10G9.3a; WBGene00010757.
GeneIDi259529.
UCSCiK10G9.3. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF455271 mRNA. Translation: AAL65193.1.
Z36282 Genomic DNA. Translation: CAD45602.1.
RefSeqiNP_001255070.1. NM_001268141.1.
UniGeneiCel.9168.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FOEX-ray1.98A/B1-424[»]
ProteinModelPortaliQ8WR51.
SMRiQ8WR51.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.K10G9.3b.

Protein family/group databases

CAZyiGT68. Glycosyltransferase Family 68.

Proteomic databases

EPDiQ8WR51.
PaxDbiQ8WR51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiK10G9.3a; K10G9.3a; WBGene00010757.
GeneIDi259529.
UCSCiK10G9.3. c. elegans.

Organism-specific databases

CTDi259529.
WormBaseiK10G9.3a; CE31715; WBGene00010757; pad-2.

Phylogenomic databases

eggNOGiENOG410IFPR. Eukaryota.
ENOG410Y78N. LUCA.
GeneTreeiENSGT00390000007989.
HOGENOMiHOG000015874.
InParanoidiQ8WR51.
PhylomeDBiQ8WR51.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-CEL-5173214. O-glycosylation of TSR domain-containing proteins.

Miscellaneous databases

PROiQ8WR51.

Gene expression databases

BgeeiWBGene00010757.
ExpressionAtlasiQ8WR51. differential.

Family and domain databases

InterProiIPR019378. GDP-Fuc_O-FucTrfase.
[Graphical view]
PfamiPF10250. O-FucT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOFUT2_CAEEL
AccessioniPrimary (citable) accession number: Q8WR51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.