Q8WQL7 (MBK1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity tyrosine-phosphorylation-regulated kinase mbk-1
EC=2.7.12.1

Alternative name(s):
Dual specificity Yak1-related kinase mbk-1
Minibrain Kinase 1

Gene names

Name:	mbk-1
ORF Names:	T04C10.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	882 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible role in the function of olfactory neurons. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.1
Cofactor	Magnesium By similarity. Ref.1
Subcellular location	Nucleus Ref.1.
Tissue specificity	Expressed in all somatic cells. Ref.1
Developmental stage	First observed during early cell morphogenesis in the embryo, levels increase during later embryonic development and remain at comparable levels throughout larval and adult stages. Ref.1
Disruption phenotype	No visible phenotype. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Olfaction Sensory transduction
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	locomotion Inferred from mutant phenotype. Source: WormBase positive regulation of growth rate Inferred from mutant phenotype. Source: WormBase response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of smell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine/tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
gei-18	Q7K7J0	3	EBI-329013,EBI-2315822

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 882	882	Dual specificity tyrosine-phosphorylation-regulated kinase mbk-1		PRO_0000390715
Regions
	Domain	328 – 649	322	Protein kinase
	Nucleotide binding	334 – 342	9	ATP By similarity UniProtKB P28523
	Compositional bias	290 – 295	6	Poly-Gln
	Compositional bias	668 – 672	5	Poly-Gln
	Compositional bias	738 – 857	120	Gln-rich
Sites
	Active site	456	1	Proton acceptor By similarity UniProtKB P28523
	Binding site	357	1	ATP By similarity UniProtKB Q9XTF3

Sequences

	Sequence		Length	Mass (Da)
	Q8WQL7 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: DD5C9DD354DA7098	FASTA	882	101,244
	10 20 30 40 50 60 MNTADVPDNL QSWGQQPSSS YSNTQQHSQM TNLPPINHNN LCDTEPMNED FQLQDVQADE 70 80 90 100 110 120 LQKQQKQQEQ QHIQQQNAQR FIAQSRQPHS NILRFPQPPI TSIKTNSHAF YPQQEVTQVR 130 140 150 160 170 180 PKKHRVSMTN AEAALTPGMP PEKQAAKKRN IGQFSTDTVP AGIGMIGIPF ESTSKPLQIQ 190 200 210 220 230 240 QFRNPQDAPV RKLTSDLIKT YKAINESFYL RKQVRRDRHK SQDAGKPKGS KDGSGASLTD 250 260 270 280 290 300 TFSIHNAIPI TSSDNHYQQD AHQNAPPLLD TNAPPTSTMV VPMRTETDLQ QQQRQKSSRG 310 320 330 340 350 360 GPYNNGYDDQ NYDYILKNGE IFDKRYVILS DTPVGKGSFG QVTKAYDTLN KEEVAIKIIK 370 380 390 400 410 420 NKKTFFDQAQ IEIHLLELTN AHDKDNKYNI VTLKGHFVHR AHLCLVFELL SYNLYDLLKN 430 440 450 460 470 480 TSFRGVSLNL ARKFAQQLGK TLLFLSSPEL SIIHCDLKPE NVLLVNAKRS QIRVIDFGSS 490 500 510 520 530 540 CQTGHRIYQY IQSRFYRSPE VLLGIAYDTK IDMWSLGCIL VEMHTGEPLF AGSSEVDQMM 550 560 570 580 590 600 KIVEVLGMPP KEMLDIGPKT HKYFDKTEDG IYYCKKTRDG YRHTYKAPGA RKLHEILGVT 610 620 630 640 650 660 SGGPGGRRLG EPGHSVEDYS KFKDLIKRML QFDPKQRISP YYVVRHPFLK QKEERVPSQP 670 680 690 700 710 720 PVSHSNLQQQ QQLYIQQPSS QMSQVMESPS VGSVYVEDNG MYRQAPGSSA NPISVTSSFD 730 740 750 760 770 780 EGDAMEVDAG RRRFSAHQQN YHNPNYQYSQ PQQQQQQQYQ QTQRTQLEQQ QKQAQLQQQL 790 800 810 820 830 840 QQQQMQQQQQ QQQQRQHMPQ AQSSSQQHLQ SRARPRQQDQ NEWRNQFELD DTFQQKQRKV 850 860 870 880 DDSVSNQISR NQFNPQQVSM THGNVNANNR EMEKLDYPNN KL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of Caenorhabditis elegans homologs of the Down syndrome candidate gene DYRK1A." Raich W.B., Moorman C., Lacefield C.O., Lehrer J., Bartsch D., Plasterk R.H., Kandel E.R., Hobert O. Genetics 163:571-580(2003) [PubMed: 12618396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY064464 mRNA. Translation: AAL40874.1. Z69885 Genomic DNA. Translation: CAA93756.2.
PIR	T24445.
RefSeq	NP_510460.2. NM_078059.3.
UniGene	Cel.22462.
3D structure databases
HSSP	HSSP built from PDB template 1H0V based on UniProtKB P24941.
ProteinModelPortal	Q8WQL7.
SMR	Q8WQL7. Positions 315-651.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8WQL7. 2 interactions.
MINT	MINT-1127171.
STRING	Q8WQL7.
Proteomic databases
PRIDE	Q8WQL7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T04C10.1; T04C10.1; T04C10.1.
GeneID	181578.
KEGG	cel:T04C10.1.
UCSC	T04C10.1. c. elegans.
Organism-specific databases
CTD	181578.
WormBase	T04C10.1; CE33442; WBGene00003149; mbk-1.
Phylogenomic databases
eggNOG	meNOG05174.
GeneTree	EMGT00050000001142.
HOGENOM	HBG389333.
OMA	VPMRTET.
PhylomeDB	Q8WQL7.
Gene expression databases
ArrayExpress	Q8WQL7.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08825.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	914518.

Entry information

Entry name

MBK1_CAEEL

Accession

Primary (citable) accession number: Q8WQL7
Secondary accession number(s): Q22155

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 19, 2010
Last sequence update:	March 1, 2002
Last modified:	November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project