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Q8WQG9 (JNK1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Stress-activated protein kinase jnk-1

EC=2.7.11.24
Gene names
Name:jnk-1
ORF Names:B0478.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responds to activation by environmental stress by phosphorylating a number of transcription factors, and thus regulates transcriptional activity By similarity. May coordinate locomotion by acting via type-D GABAergic motor neurons and regulate synaptic vesicle transport in conjunction with unc-16. Ref.1 Ref.3 UniProtKB P45983

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium. Ref.1

Enzyme regulation

Activated by threonine and tyrosine phosphorylation by either of the dual specificity kinases, jkk-1 and sek-1. Ref.1

Subunit structure

Binds to the scaffolding protein, unc-16. Unc-16 also binds other components of the JNK signaling pathway. Ref.3

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-276 and Tyr-278, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processJNK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

JUN phosphorylation

Inferred from direct assay Ref.1. Source: WormBase

determination of adult lifespan

Inferred from mutant phenotype PubMed 15767565. Source: WormBase

hyperosmotic response

Inferred from genetic interaction Ref.1. Source: WormBase

protein phosphorylation

Inferred from direct assay PubMed 15767565. Source: WormBase

response to heat

Inferred from mutant phenotype PubMed 15767565. Source: WormBase

vesicle transport along actin filament

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular_componentaxon

Inferred from direct assay Ref.1. Source: WormBase

cytoplasm

Inferred from direct assay Ref.1. Source: WormBase

neuronal cell body

Inferred from direct assay Ref.1. Source: WormBase

nucleus

Inferred from direct assay Ref.1. Source: WormBase

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

JUN kinase activity

Inferred from direct assay Ref.1. Source: WormBase

protein binding

Inferred from physical interaction Ref.1. Source: UniProtKB

protein kinase activity

Inferred from direct assay PubMed 15767565. Source: WormBase

transcription factor binding

Inferred from physical interaction PubMed 15767565. Source: WormBase

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

JUNP054123EBI-321822,EBI-852823From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q8WQG9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q8WQG9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 463463Stress-activated protein kinase jnk-1
PRO_0000186270

Regions

Domain119 – 412294Protein kinase UniProtKB P53779
Nucleotide binding126 – 1316ATP By similarity UniProtKB P53779
Motif276 – 2783TXY

Sites

Active site2441Proton acceptor By similarity UniProtKB P53779
Binding site1481ATP By similarity UniProtKB P53779

Amino acid modifications

Modified residue2761Phosphothreonine By similarity UniProtKB P53779
Modified residue2781Phosphotyrosine By similarity UniProtKB P53779

Natural variations

Alternative sequence1 – 9191Missing in isoform b.
VSP_050269

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified October 1, 2002. Version 2.
Checksum: E12495B45B052BC7

FASTA46352,885
        10         20         30         40         50         60 
MEERLSTTSS YPSHPGRSVE EDHNTLLASS SISSIIRGTR GHLNNFIESV GNWLVPSSSG 

        70         80         90        100        110        120 
RDDDAVSLDS CQSVYSPVRH HINSGTGGGI LMEPSSIHVP ENYYSVTIGE AQMVVLKRYQ 

       130        140        150        160        170        180 
NLRLIGSGAQ GIVCSAFDTV RNEQVAIKKL SRPFQNVTHA KRAYRELKLM SLVNHKNIIG 

       190        200        210        220        230        240 
ILNCFTPQKK LDEFNDLYIV MELMDANLCQ VIQMDLDHER LSYLLYQMLC GIRHLHSAGI 

       250        260        270        280        290        300 
IHRDLKPSNI VVRSDCTLKI LDFGLARTAI EAFMMTPYVV TRYYRAPEVI LGMGYKENVD 

       310        320        330        340        350        360 
VWSIGCIFGE LIRGRVLFPG GDHIDQWTRI IEQLGTPDRS FLERLQPTVR NYVENRPRYQ 

       370        380        390        400        410        420 
ATPFEVLFSD NMFPMTADSS RLTGAQARDL LSRMLVIDPE RRISVDDALR HPYVNVWFDE 

       430        440        450        460 
IEVYAPPPLP YDHNMDVEQN VDSWREHIFR ELTDYARTHD IYS 

« Hide

Isoform b [UniParc].

Checksum: D923F874F36F9856
Show »

FASTA37243,179

References

« Hide 'large scale' references
[1]"A Caenorhabditis elegans JNK signal transduction pathway regulates coordinated movement via type-D GABAergic motor neurons."
Kawasaki M., Hisamoto N., Iino Y., Yamamoto M., Ninomiya-Tsuji J., Matsumoto K.
EMBO J. 18:3604-3615(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, COFACTOR, ENZYME REGULATION.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
[3]"UNC-16, a JNK-signaling scaffold protein, regulates vesicle transport in C. elegans."
Byrd D.T., Kawasaki M., Walcoff M., Hisamoto N., Matsumoto K., Jin Y.
Neuron 32:787-800(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB024085 mRNA. Translation: BAA82640.1.
FO080209 Genomic DNA. Translation: CCD62004.1.
FO080209 Genomic DNA. Translation: CCD62005.1.
PIRT30031.
T37323.
RefSeqNP_001021270.1. NM_001026099.3. [Q8WQG9-1]
NP_741434.2. NM_171371.4. [Q8WQG9-2]
UniGeneCel.18197.

3D structure databases

ProteinModelPortalQ8WQG9.
SMRQ8WQG9. Positions 103-454.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid42580. 2 interactions.
DIPDIP-26951N.
IntActQ8WQG9. 4 interactions.
MINTMINT-1087649.
STRING6239.B0478.1a.

Proteomic databases

PaxDbQ8WQG9.
PRIDEQ8WQG9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaB0478.1a; B0478.1a; WBGene00002178. [Q8WQG9-1]
GeneID177460.
KEGGcel:CELE_B0478.1.
UCSCB0478.1a. c. elegans. [Q8WQG9-1]

Organism-specific databases

CTD177460.
WormBaseB0478.1a; CE27574; WBGene00002178; jnk-1.
B0478.1b; CE30123; WBGene00002178; jnk-1.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074271.
HOGENOMHOG000233024.
InParanoidQ8WQG9.
KOK04440.
OMAREHTLEQ.
PhylomeDBQ8WQG9.

Enzyme and pathway databases

BRENDA2.7.11.24. 1045.
SignaLinkQ8WQG9.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01772. JNKMAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio896922.

Entry information

Entry nameJNK1_CAEEL
AccessionPrimary (citable) accession number: Q8WQG9
Secondary accession number(s): Q17507, Q9UAH2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase