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Q8WQG9

- JNK1_CAEEL

UniProt

Q8WQG9 - JNK1_CAEEL

Protein

Stress-activated protein kinase jnk-1

Gene

jnk-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Responds to activation by environmental stress by phosphorylating a number of transcription factors, and thus regulates transcriptional activity By similarity. May coordinate locomotion by acting via type-D GABAergic motor neurons and regulate synaptic vesicle transport in conjunction with unc-16.By similarity2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.1 Publication

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation by either of the dual specificity kinases, jkk-1 and sek-1.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei148 – 1481ATPPROSITE-ProRule annotation
    Active sitei244 – 2441Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi126 – 1316ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. JUN kinase activity Source: WormBase
    3. protein binding Source: UniProtKB
    4. protein kinase activity Source: WormBase
    5. transcription factor binding Source: WormBase

    GO - Biological processi

    1. determination of adult lifespan Source: WormBase
    2. hyperosmotic response Source: WormBase
    3. JNK cascade Source: UniProtKB
    4. JUN phosphorylation Source: WormBase
    5. protein phosphorylation Source: WormBase
    6. response to heat Source: WormBase
    7. vesicle transport along actin filament Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 1045.
    ReactomeiREACT_184089. FCERI mediated MAPK activation.
    REACT_184347. Oxidative Stress Induced Senescence.
    SignaLinkiQ8WQG9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Stress-activated protein kinase jnk-1 (EC:2.7.11.24)
    Gene namesi
    Name:jnk-1
    ORF Names:B0478.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome IV

    Organism-specific databases

    WormBaseiB0478.1a; CE27574; WBGene00002178; jnk-1.
    B0478.1b; CE30123; WBGene00002178; jnk-1.

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: WormBase
    2. cytoplasm Source: WormBase
    3. neuronal cell body Source: WormBase
    4. nucleus Source: WormBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 463463Stress-activated protein kinase jnk-1PRO_0000186270Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei276 – 2761PhosphothreonineBy similarity
    Modified residuei278 – 2781PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-276 and Tyr-278, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8WQG9.
    PRIDEiQ8WQG9.

    Interactioni

    Subunit structurei

    Binds to the scaffolding protein, unc-16. Unc-16 also binds other components of the JNK signaling pathway.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    JUNP054123EBI-321822,EBI-852823From a different organism.

    Protein-protein interaction databases

    BioGridi42580. 2 interactions.
    DIPiDIP-26951N.
    IntActiQ8WQG9. 4 interactions.
    MINTiMINT-1087649.
    STRINGi6239.B0478.1a.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8WQG9.
    SMRiQ8WQG9. Positions 103-454.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini119 – 412294Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi276 – 2783TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074271.
    HOGENOMiHOG000233024.
    InParanoidiQ8WQG9.
    KOiK04440.
    OMAiREHTLEQ.
    PhylomeDBiQ8WQG9.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008351. MAPK_JNK.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01772. JNKMAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: Q8WQG9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEERLSTTSS YPSHPGRSVE EDHNTLLASS SISSIIRGTR GHLNNFIESV    50
    GNWLVPSSSG RDDDAVSLDS CQSVYSPVRH HINSGTGGGI LMEPSSIHVP 100
    ENYYSVTIGE AQMVVLKRYQ NLRLIGSGAQ GIVCSAFDTV RNEQVAIKKL 150
    SRPFQNVTHA KRAYRELKLM SLVNHKNIIG ILNCFTPQKK LDEFNDLYIV 200
    MELMDANLCQ VIQMDLDHER LSYLLYQMLC GIRHLHSAGI IHRDLKPSNI 250
    VVRSDCTLKI LDFGLARTAI EAFMMTPYVV TRYYRAPEVI LGMGYKENVD 300
    VWSIGCIFGE LIRGRVLFPG GDHIDQWTRI IEQLGTPDRS FLERLQPTVR 350
    NYVENRPRYQ ATPFEVLFSD NMFPMTADSS RLTGAQARDL LSRMLVIDPE 400
    RRISVDDALR HPYVNVWFDE IEVYAPPPLP YDHNMDVEQN VDSWREHIFR 450
    ELTDYARTHD IYS 463
    Length:463
    Mass (Da):52,885
    Last modified:October 1, 2002 - v2
    Checksum:iE12495B45B052BC7
    GO
    Isoform b (identifier: Q8WQG9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-91: Missing.

    Show »
    Length:372
    Mass (Da):43,179
    Checksum:iD923F874F36F9856
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9191Missing in isoform b. CuratedVSP_050269Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB024085 mRNA. Translation: BAA82640.1.
    FO080209 Genomic DNA. Translation: CCD62004.1.
    FO080209 Genomic DNA. Translation: CCD62005.1.
    PIRiT30031.
    T37323.
    RefSeqiNP_001021270.1. NM_001026099.3. [Q8WQG9-1]
    NP_741434.2. NM_171371.4. [Q8WQG9-2]
    UniGeneiCel.18197.

    Genome annotation databases

    EnsemblMetazoaiB0478.1a; B0478.1a; WBGene00002178. [Q8WQG9-1]
    GeneIDi177460.
    KEGGicel:CELE_B0478.1.
    UCSCiB0478.1a. c. elegans. [Q8WQG9-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB024085 mRNA. Translation: BAA82640.1 .
    FO080209 Genomic DNA. Translation: CCD62004.1 .
    FO080209 Genomic DNA. Translation: CCD62005.1 .
    PIRi T30031.
    T37323.
    RefSeqi NP_001021270.1. NM_001026099.3. [Q8WQG9-1 ]
    NP_741434.2. NM_171371.4. [Q8WQG9-2 ]
    UniGenei Cel.18197.

    3D structure databases

    ProteinModelPortali Q8WQG9.
    SMRi Q8WQG9. Positions 103-454.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 42580. 2 interactions.
    DIPi DIP-26951N.
    IntActi Q8WQG9. 4 interactions.
    MINTi MINT-1087649.
    STRINGi 6239.B0478.1a.

    Proteomic databases

    PaxDbi Q8WQG9.
    PRIDEi Q8WQG9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai B0478.1a ; B0478.1a ; WBGene00002178 . [Q8WQG9-1 ]
    GeneIDi 177460.
    KEGGi cel:CELE_B0478.1.
    UCSCi B0478.1a. c. elegans. [Q8WQG9-1 ]

    Organism-specific databases

    CTDi 177460.
    WormBasei B0478.1a ; CE27574 ; WBGene00002178 ; jnk-1.
    B0478.1b ; CE30123 ; WBGene00002178 ; jnk-1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074271.
    HOGENOMi HOG000233024.
    InParanoidi Q8WQG9.
    KOi K04440.
    OMAi REHTLEQ.
    PhylomeDBi Q8WQG9.

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 1045.
    Reactomei REACT_184089. FCERI mediated MAPK activation.
    REACT_184347. Oxidative Stress Induced Senescence.
    SignaLinki Q8WQG9.

    Miscellaneous databases

    NextBioi 896922.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008351. MAPK_JNK.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01772. JNKMAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A Caenorhabditis elegans JNK signal transduction pathway regulates coordinated movement via type-D GABAergic motor neurons."
      Kawasaki M., Hisamoto N., Iino Y., Yamamoto M., Ninomiya-Tsuji J., Matsumoto K.
      EMBO J. 18:3604-3615(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, COFACTOR, ENZYME REGULATION.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    3. "UNC-16, a JNK-signaling scaffold protein, regulates vesicle transport in C. elegans."
      Byrd D.T., Kawasaki M., Walcoff M., Hisamoto N., Matsumoto K., Jin Y.
      Neuron 32:787-800(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.

    Entry informationi

    Entry nameiJNK1_CAEEL
    AccessioniPrimary (citable) accession number: Q8WQG9
    Secondary accession number(s): Q17507, Q9UAH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3