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Q8WQG9

- JNK1_CAEEL

UniProt

Q8WQG9 - JNK1_CAEEL

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Protein

Stress-activated protein kinase jnk-1

Gene
jnk-1, B0478.1
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Responds to activation by environmental stress by phosphorylating a number of transcription factors, and thus regulates transcriptional activity By similarity. May coordinate locomotion by acting via type-D GABAergic motor neurons and regulate synaptic vesicle transport in conjunction with unc-16.By similarity2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.1 Publication

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by either of the dual specificity kinases, jkk-1 and sek-1.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei148 – 1481ATP By similarityBy similarity
Active sitei244 – 2441Proton acceptor By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi126 – 1316ATP By similarityBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. JUN kinase activity Source: WormBase
  3. protein binding Source: UniProtKB
  4. protein kinase activity Source: WormBase
  5. transcription factor binding Source: WormBase

GO - Biological processi

  1. determination of adult lifespan Source: WormBase
  2. hyperosmotic response Source: WormBase
  3. JNK cascade Source: UniProtKB
  4. JUN phosphorylation Source: WormBase
  5. protein phosphorylation Source: WormBase
  6. response to heat Source: WormBase
  7. vesicle transport along actin filament Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 1045.
ReactomeiREACT_184089. FCERI mediated MAPK activation.
REACT_184347. Oxidative Stress Induced Senescence.
SignaLinkiQ8WQG9.

Names & Taxonomyi

Protein namesi
Recommended name:
Stress-activated protein kinase jnk-1 (EC:2.7.11.24)
Gene namesi
Name:jnk-1
ORF Names:B0478.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiB0478.1a; CE27574; WBGene00002178; jnk-1.
B0478.1b; CE30123; WBGene00002178; jnk-1.

Subcellular locationi

GO - Cellular componenti

  1. axon Source: WormBase
  2. cytoplasm Source: WormBase
  3. neuronal cell body Source: WormBase
  4. nucleus Source: WormBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Stress-activated protein kinase jnk-1PRO_0000186270Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei276 – 2761Phosphothreonine By similarityBy similarity
Modified residuei278 – 2781Phosphotyrosine By similarityBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-276 and Tyr-278, which activates the enzyme By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8WQG9.
PRIDEiQ8WQG9.

Interactioni

Subunit structurei

Binds to the scaffolding protein, unc-16. Unc-16 also binds other components of the JNK signaling pathway.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
JUNP054123EBI-321822,EBI-852823From a different organism.

Protein-protein interaction databases

BioGridi42580. 2 interactions.
DIPiDIP-26951N.
IntActiQ8WQG9. 4 interactions.
MINTiMINT-1087649.
STRINGi6239.B0478.1a.

Structurei

3D structure databases

ProteinModelPortaliQ8WQG9.
SMRiQ8WQG9. Positions 103-454.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini119 – 412294Protein kinaseBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi276 – 2783TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
HOGENOMiHOG000233024.
InParanoidiQ8WQG9.
KOiK04440.
OMAiREHTLEQ.
PhylomeDBiQ8WQG9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: Q8WQG9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEERLSTTSS YPSHPGRSVE EDHNTLLASS SISSIIRGTR GHLNNFIESV    50
GNWLVPSSSG RDDDAVSLDS CQSVYSPVRH HINSGTGGGI LMEPSSIHVP 100
ENYYSVTIGE AQMVVLKRYQ NLRLIGSGAQ GIVCSAFDTV RNEQVAIKKL 150
SRPFQNVTHA KRAYRELKLM SLVNHKNIIG ILNCFTPQKK LDEFNDLYIV 200
MELMDANLCQ VIQMDLDHER LSYLLYQMLC GIRHLHSAGI IHRDLKPSNI 250
VVRSDCTLKI LDFGLARTAI EAFMMTPYVV TRYYRAPEVI LGMGYKENVD 300
VWSIGCIFGE LIRGRVLFPG GDHIDQWTRI IEQLGTPDRS FLERLQPTVR 350
NYVENRPRYQ ATPFEVLFSD NMFPMTADSS RLTGAQARDL LSRMLVIDPE 400
RRISVDDALR HPYVNVWFDE IEVYAPPPLP YDHNMDVEQN VDSWREHIFR 450
ELTDYARTHD IYS 463
Length:463
Mass (Da):52,885
Last modified:October 1, 2002 - v2
Checksum:iE12495B45B052BC7
GO
Isoform b (identifier: Q8WQG9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-91: Missing.

Show »
Length:372
Mass (Da):43,179
Checksum:iD923F874F36F9856
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9191Missing in isoform b. VSP_050269Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB024085 mRNA. Translation: BAA82640.1.
FO080209 Genomic DNA. Translation: CCD62004.1.
FO080209 Genomic DNA. Translation: CCD62005.1.
PIRiT30031.
T37323.
RefSeqiNP_001021270.1. NM_001026099.3. [Q8WQG9-1]
NP_741434.2. NM_171371.4. [Q8WQG9-2]
UniGeneiCel.18197.

Genome annotation databases

EnsemblMetazoaiB0478.1a; B0478.1a; WBGene00002178. [Q8WQG9-1]
GeneIDi177460.
KEGGicel:CELE_B0478.1.
UCSCiB0478.1a. c. elegans. [Q8WQG9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB024085 mRNA. Translation: BAA82640.1 .
FO080209 Genomic DNA. Translation: CCD62004.1 .
FO080209 Genomic DNA. Translation: CCD62005.1 .
PIRi T30031.
T37323.
RefSeqi NP_001021270.1. NM_001026099.3. [Q8WQG9-1 ]
NP_741434.2. NM_171371.4. [Q8WQG9-2 ]
UniGenei Cel.18197.

3D structure databases

ProteinModelPortali Q8WQG9.
SMRi Q8WQG9. Positions 103-454.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 42580. 2 interactions.
DIPi DIP-26951N.
IntActi Q8WQG9. 4 interactions.
MINTi MINT-1087649.
STRINGi 6239.B0478.1a.

Proteomic databases

PaxDbi Q8WQG9.
PRIDEi Q8WQG9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai B0478.1a ; B0478.1a ; WBGene00002178 . [Q8WQG9-1 ]
GeneIDi 177460.
KEGGi cel:CELE_B0478.1.
UCSCi B0478.1a. c. elegans. [Q8WQG9-1 ]

Organism-specific databases

CTDi 177460.
WormBasei B0478.1a ; CE27574 ; WBGene00002178 ; jnk-1.
B0478.1b ; CE30123 ; WBGene00002178 ; jnk-1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074271.
HOGENOMi HOG000233024.
InParanoidi Q8WQG9.
KOi K04440.
OMAi REHTLEQ.
PhylomeDBi Q8WQG9.

Enzyme and pathway databases

BRENDAi 2.7.11.24. 1045.
Reactomei REACT_184089. FCERI mediated MAPK activation.
REACT_184347. Oxidative Stress Induced Senescence.
SignaLinki Q8WQG9.

Miscellaneous databases

NextBioi 896922.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01772. JNKMAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Caenorhabditis elegans JNK signal transduction pathway regulates coordinated movement via type-D GABAergic motor neurons."
    Kawasaki M., Hisamoto N., Iino Y., Yamamoto M., Ninomiya-Tsuji J., Matsumoto K.
    EMBO J. 18:3604-3615(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, COFACTOR, ENZYME REGULATION.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  3. "UNC-16, a JNK-signaling scaffold protein, regulates vesicle transport in C. elegans."
    Byrd D.T., Kawasaki M., Walcoff M., Hisamoto N., Matsumoto K., Jin Y.
    Neuron 32:787-800(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.

Entry informationi

Entry nameiJNK1_CAEEL
AccessioniPrimary (citable) accession number: Q8WQG9
Secondary accession number(s): Q17507, Q9UAH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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