SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8WP17

- KLOT_MACFA

UniProt

Q8WP17 - KLOT_MACFA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Klotho
Gene
KL
Organism
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D By similarity. Essential factor for the specific interaction between FGF23 and FGFR1 By similarity.
The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling By similarity.

Catalytic activityi

A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

GO - Molecular functioni

  1. beta-glucuronidase activity Source: UniProtKB-EC
  2. signal transducer activity Source: InterPro

GO - Biological processi

  1. aging Source: InterPro
  2. carbohydrate metabolic process Source: InterPro
  3. fibroblast growth factor receptor signaling pathway Source: InterPro
  4. insulin receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Klotho (EC:3.2.1.31)
Cleaved into the following chain:
Gene namesi
Name:KL
OrganismiMacaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Taxonomic identifieri9541 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Isoform 1 : Cell membrane; Single-pass type I membrane protein By similarity
Note: Isoform 1 shedding leads to a soluble peptide.
Isoform 2 : Secreted By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini36 – 983948Extracellular Reviewed prediction
Add
BLAST
Transmembranei984 – 100421Helical; Reviewed prediction
Add
BLAST
Topological domaini1005 – 101410Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. extracellular space Source: InterPro
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535 Reviewed prediction
Add
BLAST
Chaini36 – 1014979Klotho
PRO_0000042245Add
BLAST
Chaini36 – ?Klotho peptide By similarityPRO_0000042246

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi161 – 1611N-linked (GlcNAc...) Reviewed prediction
Glycosylationi285 – 2851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi346 – 3461N-linked (GlcNAc...) Reviewed prediction
Glycosylationi609 – 6091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi614 – 6141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi696 – 6961N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homodimer By similarity. Interacts with FGF23 and FGFR1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ8WP17.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni59 – 508450Glycosyl hydrolase-1 1
Add
BLAST
Regioni517 – 955439Glycosyl hydrolase-1 2
Add
BLAST

Domaini

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG081856.

Family and domain databases

Gene3Di3.20.20.80. 2 hits.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028546. Klotho.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PTHR10353:SF10. PTHR10353:SF10. 1 hit.
PfamiPF00232. Glyco_hydro_1. 3 hits.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 2 hits.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8WP17-1) [UniParc]FASTAAdd to Basket

Also known as: Membrane-bound

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPASAPPRRP RPPPPSLSLS LLLVLLGLAG RRLRAEPGDG AQTWARFARP     50
PAPEAAGLFQ GTFPDGFLWA VGSAAYQTEG GWQQHGKGAS IWDTFTHHPL 100
APPGDSRIAN VPSGAPSPLQ PATGDVASDS YNNVFRDTEA LRELGVTHYR 150
FSISWARVLP NGSAGVPNRE GLRYYRRLLE RLRELGVQPV VTLYHWDLPQ 200
RLQDAYGGWA NRALADHFRD YAELCFRHFG GQVKYWITID NPYVVAWHGY 250
ATGRLAPGIR GSPRLGYLVA HNLLLAHAKV WHLYNTSFRP TQGGQVSIAL 300
SSHWINPRRM TDHSIKECQK SLDFVLGWFA KPIFIDGDYP ESMKNNLSSL 350
LPDFTESEKK FIKGTADFFA LSFGPTLSFQ LLDPHMKFRQ LESPSLRQLL 400
SWIDLEYNHP QIFIVENGWF VSGTTKRDDA KYMYYLKKFI METLKAIKLD 450
GVDVIGYTAW SLMDGFEWHR GYSIRRGLFY VDFLSQEKTL LPKSSALFYQ 500
KLIEKNGFPP LPENQPLEGT FPCDFAWGIV DNYIQVDTTL SQFTDLNVYL 550
WDVHHSKRLI KVDGVVTKKR KSYCVDFAAI QPQITLLQEM HVTHFRFSLD 600
WALILPLGNQ SQVNHTILQY YRCMVSELVR VNITPVVALW QPVAPNQGLP 650
RLLARQGAWE NPYTALAFAE YARLCFQELG HHVKLWITMN EPYTRNMTYS 700
AGHNLLKAHA LAWHVYNEKF RHAQNGKISI ALQADWIEPA CPFSQKDKEV 750
AERVLEFDIG WLAEPIFGSG DYPWVMRDWL NQRNNFLLPY FTEDEKKLIQ 800
GTFDFLALSH YTTILVDSEK EDPIKYNDYL EVQEMTDITW LNSPSQVAVV 850
PWGLRKVLNW LKFKYGDLPM YIISNGIDDG LHAEDDQLRV YYMQNYINEA 900
LKAHILDGIN LCGYFAYSFN DRTAPRFGLY RFAADQFEPK PSMKHYRKII 950
DSNGFPGPET LEKFCPEEFT VCTECSFFHT RKPLVAFIAF LFFAFIVSLS 1000
LIFYYSKKGR RRYQ 1014
Length:1,014
Mass (Da):116,469
Last modified:March 1, 2002 - v1
Checksum:i00669CD244A4DD0F
GO
Isoform 2 (identifier: Q8WP17-2) [UniParc]FASTAAdd to Basket

Also known as: Secreted

The sequence of this isoform differs from the canonical sequence as follows:
     537-551: DTTLSQFTDLNVYLW → SQLAKPISSLTKPYH
     552-1014: Missing.

Show »
Length:551
Mass (Da):62,274
Checksum:i45F4EAD2358DDBAA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei537 – 55115DTTLS…NVYLW → SQLAKPISSLTKPYH in isoform 2.
VSP_015826Add
BLAST
Alternative sequencei552 – 1014463Missing in isoform 2.
VSP_015827Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF064053 mRNA. Translation: AAC77917.1.
AF064054 mRNA. Translation: AAC77918.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

Protein Spotlight

The thread of life - Issue 65 of December 2005

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF064053 mRNA. Translation: AAC77917.1 .
AF064054 mRNA. Translation: AAC77918.1 .

3D structure databases

ProteinModelPortali Q8WP17.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG081856.

Family and domain databases

Gene3Di 3.20.20.80. 2 hits.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028546. Klotho.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
PTHR10353:SF10. PTHR10353:SF10. 1 hit.
Pfami PF00232. Glyco_hydro_1. 3 hits.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 2 hits.
PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Koh N., Fujimori T., Nabeshima Y.
    Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).

Entry informationi

Entry nameiKLOT_MACFA
AccessioniPrimary (citable) accession number: Q8WP17
Secondary accession number(s): Q8WP18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi