Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8WP15

- GRK7_PIG

UniProt

Q8WP15 - GRK7_PIG

Protein

G protein-coupled receptor kinase 7

Gene

GRK7

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).By similarity

    Catalytic activityi

    ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
    ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.

    Enzyme regulationi

    Inhibited by phosphorylation of Ser-36.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei220 – 2201ATPPROSITE-ProRule annotation
    Active sitei316 – 3161Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi197 – 2059ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
    3. rhodopsin kinase activity Source: UniProtKB

    GO - Biological processi

    1. protein autophosphorylation Source: Ensembl
    2. signal transduction Source: InterPro
    3. termination of G-protein coupled receptor signaling pathway Source: InterPro
    4. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    G protein-coupled receptor kinase 7 (EC:2.7.11.14, EC:2.7.11.16)
    Alternative name(s):
    G protein-coupled receptor kinase GRK7
    Gene namesi
    Name:GRK7
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 13

    Subcellular locationi

    Membrane By similarity; Lipid-anchor By similarity

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 550550G protein-coupled receptor kinase 7PRO_0000024334Add
    BLAST
    Propeptidei551 – 5533Removed in mature formBy similarityPRO_0000024335

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361Phosphoserine; by PKA1 Publication
    Modified residuei550 – 5501Cysteine methyl esterSequence Analysis
    Lipidationi550 – 5501S-geranylgeranyl cysteineSequence Analysis

    Post-translational modificationi

    Autophosphorylated in vitro at Ser-490 By similarity. Phosphorylation at Ser-36 is regulated by light and activated by cAMP.By similarity1 Publication

    Keywords - PTMi

    Lipoprotein, Methylation, Phosphoprotein, Prenylation

    Interactioni

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000012443.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8WP15.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 176121RGSPROSITE-ProRule annotationAdd
    BLAST
    Domaini191 – 454264Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini455 – 52066AGC-kinase C-terminalAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RGS domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00730000110751.
    HOGENOMiHOG000006742.
    HOVERGENiHBG004532.
    KOiK00909.
    OMAiAYAFESK.
    OrthoDBiEOG7V1FQK.
    TreeFamiTF313940.

    Family and domain databases

    InterProiIPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view]
    PRINTSiPR00717. GPCRKINASE.
    SMARTiSM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8WP15-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVDMGGLDNL IANTAYLQAR KTSDADSKEL QRRRRSLMLP GPQSCEQLRQ    50
    AMPADFNSLC EQQPIGRRLF RDFLATVPAY QEAMGFLEEV QSWELAEEGP 100
    AKGSTLQALV ATCAVAPNPG QPHSFLSPAL VTKCQAATTD EERASLVEQA 150
    KAEAMAFLQD QPFREFLVSP FYDKFLQWKV FEMQPVSDKY FEEFRVLGKG 200
    GFGEVCAVQV KNTGKMYACK KLDKKRLKKK SGEKMALSEK EILEKVSSPF 250
    VVSLAYAFES KSHLCLVMSL MNGGDLKFHI YSVGERGLDM NRVIFYSAQM 300
    TCGVLHLHSL GIVYRDLKPE NVLLDDLGNC RLSDLGLAVQ IQDGKPVTQR 350
    AGTNGYMAPE ILMEKASYSY PVDWFAMGCS IYEMVAGRTP FKDYKEKISK 400
    EDLKQRTLKE EVRFQHQSFT EEAKDICRLF LAKTPEQRLG SREKSDDPRK 450
    HHFFKTINFP RLEAGLVDPP FVPDPSVVYA KDVDEIEDFS EVRGVEFDDK 500
    DKQFFQRFAT GAVPIAWQEE IIETGLFEEL NDPNRPAGCG EGNSSRSGVC 550
    LLL 553
    Length:553
    Mass (Da):62,299
    Last modified:March 1, 2002 - v1
    Checksum:i9BAD6E91BF31D33E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF282270 mRNA. Translation: AAL33881.1.
    RefSeqiNP_999212.1. NM_214047.1.
    UniGeneiSsc.165.

    Genome annotation databases

    EnsembliENSSSCT00000012780; ENSSSCP00000012443; ENSSSCG00000011675.
    GeneIDi397114.
    KEGGissc:397114.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF282270 mRNA. Translation: AAL33881.1 .
    RefSeqi NP_999212.1. NM_214047.1.
    UniGenei Ssc.165.

    3D structure databases

    ProteinModelPortali Q8WP15.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000012443.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000012780 ; ENSSSCP00000012443 ; ENSSSCG00000011675 .
    GeneIDi 397114.
    KEGGi ssc:397114.

    Organism-specific databases

    CTDi 131890.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00730000110751.
    HOGENOMi HOG000006742.
    HOVERGENi HBG004532.
    KOi K00909.
    OMAi AYAFESK.
    OrthoDBi EOG7V1FQK.
    TreeFami TF313940.

    Family and domain databases

    InterProi IPR000961. AGC-kinase_C.
    IPR000239. GPCR_kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR016137. Regulat_G_prot_signal_superfam.
    IPR000342. RGS_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF00615. RGS. 1 hit.
    [Graphical view ]
    PRINTSi PR00717. GPCRKINASE.
    SMARTi SM00315. RGS. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48097. SSF48097. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50132. RGS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Species-specific differences in expression of G-protein-coupled receptor kinase (GRK) 7 and GRK1 in mammalian cone photoreceptor cells: implications for cone cell phototransduction."
      Weiss E.R., Ducceschi M.H., Horner T.J., Li A., Craft C.M., Osawa S.
      J. Neurosci. 21:9175-9184(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    2. "Phosphorylation of GRK7 by PKA in cone photoreceptor cells is regulated by light."
      Osawa S., Jo R., Weiss E.R.
      J. Neurochem. 107:1314-1324(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-36.

    Entry informationi

    Entry nameiGRK7_PIG
    AccessioniPrimary (citable) accession number: Q8WP15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3