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Protein

G protein-coupled receptor kinase 7

Gene

GRK7

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).By similarity

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.By similarity

Enzyme regulationi

Inhibited by phosphorylation of Ser-36.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201ATPPROSITE-ProRule annotation
Active sitei316 – 3161Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi197 – 2059ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 7 (EC:2.7.11.14By similarity, EC:2.7.11.16)
Alternative name(s):
G protein-coupled receptor kinase GRK7
Gene namesi
Name:GRK7
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Chromosome 13

Subcellular locationi

  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 550550G protein-coupled receptor kinase 7PRO_0000024334Add
BLAST
Propeptidei551 – 5533Removed in mature formBy similarityPRO_0000024335

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361Phosphoserine; by PKA1 Publication
Modified residuei550 – 5501Cysteine methyl esterSequence Analysis
Lipidationi550 – 5501S-geranylgeranyl cysteineSequence Analysis

Post-translational modificationi

Autophosphorylated in vitro at Ser-490 (By similarity). Phosphorylation at Ser-36 is regulated by light and activated by cAMP.By similarity1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Interactioni

Subunit structurei

Interacts (when prenylated) with PDE6D; this promotes release from membranes.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000012443.

Structurei

3D structure databases

ProteinModelPortaliQ8WP15.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 176121RGSPROSITE-ProRule annotationAdd
BLAST
Domaini191 – 454264Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini455 – 52066AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120493.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ8WP15.
KOiK00909.
OMAiESKSHLC.
OrthoDBiEOG7V1FQK.
TreeFamiTF313940.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8WP15-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDMGGLDNL IANTAYLQAR KTSDADSKEL QRRRRSLMLP GPQSCEQLRQ
60 70 80 90 100
AMPADFNSLC EQQPIGRRLF RDFLATVPAY QEAMGFLEEV QSWELAEEGP
110 120 130 140 150
AKGSTLQALV ATCAVAPNPG QPHSFLSPAL VTKCQAATTD EERASLVEQA
160 170 180 190 200
KAEAMAFLQD QPFREFLVSP FYDKFLQWKV FEMQPVSDKY FEEFRVLGKG
210 220 230 240 250
GFGEVCAVQV KNTGKMYACK KLDKKRLKKK SGEKMALSEK EILEKVSSPF
260 270 280 290 300
VVSLAYAFES KSHLCLVMSL MNGGDLKFHI YSVGERGLDM NRVIFYSAQM
310 320 330 340 350
TCGVLHLHSL GIVYRDLKPE NVLLDDLGNC RLSDLGLAVQ IQDGKPVTQR
360 370 380 390 400
AGTNGYMAPE ILMEKASYSY PVDWFAMGCS IYEMVAGRTP FKDYKEKISK
410 420 430 440 450
EDLKQRTLKE EVRFQHQSFT EEAKDICRLF LAKTPEQRLG SREKSDDPRK
460 470 480 490 500
HHFFKTINFP RLEAGLVDPP FVPDPSVVYA KDVDEIEDFS EVRGVEFDDK
510 520 530 540 550
DKQFFQRFAT GAVPIAWQEE IIETGLFEEL NDPNRPAGCG EGNSSRSGVC

LLL
Length:553
Mass (Da):62,299
Last modified:March 1, 2002 - v1
Checksum:i9BAD6E91BF31D33E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282270 mRNA. Translation: AAL33881.1.
RefSeqiNP_999212.1. NM_214047.1.
UniGeneiSsc.165.

Genome annotation databases

EnsembliENSSSCT00000012780; ENSSSCP00000012443; ENSSSCG00000011675.
GeneIDi397114.
KEGGissc:397114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282270 mRNA. Translation: AAL33881.1.
RefSeqiNP_999212.1. NM_214047.1.
UniGeneiSsc.165.

3D structure databases

ProteinModelPortaliQ8WP15.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000012443.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000012780; ENSSSCP00000012443; ENSSSCG00000011675.
GeneIDi397114.
KEGGissc:397114.

Organism-specific databases

CTDi131890.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00770000120493.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ8WP15.
KOiK00909.
OMAiESKSHLC.
OrthoDBiEOG7V1FQK.
TreeFamiTF313940.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Species-specific differences in expression of G-protein-coupled receptor kinase (GRK) 7 and GRK1 in mammalian cone photoreceptor cells: implications for cone cell phototransduction."
    Weiss E.R., Ducceschi M.H., Horner T.J., Li A., Craft C.M., Osawa S.
    J. Neurosci. 21:9175-9184(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. "Phosphorylation of GRK7 by PKA in cone photoreceptor cells is regulated by light."
    Osawa S., Jo R., Weiss E.R.
    J. Neurochem. 107:1314-1324(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-36.

Entry informationi

Entry nameiGRK7_PIG
AccessioniPrimary (citable) accession number: Q8WP15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.