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Q8WP15 (GRK7_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G protein-coupled receptor kinase 7

EC=2.7.11.14
EC=2.7.11.16
Alternative name(s):
G protein-coupled receptor kinase GRK7
Gene names
Name:GRK7
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO) By similarity.

Catalytic activity

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.

ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.

Enzyme regulation

Inhibited by phosphorylation of Ser-36 By similarity.

Subcellular location

Membrane; Lipid-anchor By similarity.

Post-translational modification

Autophosphorylated in vitro at Ser-490 By similarity. Phosphorylation at Ser-36 is regulated by light and activated by cAMP. Ref.2

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 550550G protein-coupled receptor kinase 7
PRO_0000024334
Propeptide551 – 5533Removed in mature form By similarity
PRO_0000024335

Regions

Domain56 – 176121RGS
Domain191 – 454264Protein kinase
Domain455 – 52066AGC-kinase C-terminal
Nucleotide binding197 – 2059ATP By similarity

Sites

Active site3161Proton acceptor By similarity
Binding site2201ATP By similarity

Amino acid modifications

Modified residue361Phosphoserine; by PKA Ref.2
Modified residue5501Cysteine methyl ester Potential
Lipidation5501S-geranylgeranyl cysteine Potential

Sequences

Sequence LengthMass (Da)Tools
Q8WP15 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 9BAD6E91BF31D33E

FASTA55362,299
        10         20         30         40         50         60 
MVDMGGLDNL IANTAYLQAR KTSDADSKEL QRRRRSLMLP GPQSCEQLRQ AMPADFNSLC 

        70         80         90        100        110        120 
EQQPIGRRLF RDFLATVPAY QEAMGFLEEV QSWELAEEGP AKGSTLQALV ATCAVAPNPG 

       130        140        150        160        170        180 
QPHSFLSPAL VTKCQAATTD EERASLVEQA KAEAMAFLQD QPFREFLVSP FYDKFLQWKV 

       190        200        210        220        230        240 
FEMQPVSDKY FEEFRVLGKG GFGEVCAVQV KNTGKMYACK KLDKKRLKKK SGEKMALSEK 

       250        260        270        280        290        300 
EILEKVSSPF VVSLAYAFES KSHLCLVMSL MNGGDLKFHI YSVGERGLDM NRVIFYSAQM 

       310        320        330        340        350        360 
TCGVLHLHSL GIVYRDLKPE NVLLDDLGNC RLSDLGLAVQ IQDGKPVTQR AGTNGYMAPE 

       370        380        390        400        410        420 
ILMEKASYSY PVDWFAMGCS IYEMVAGRTP FKDYKEKISK EDLKQRTLKE EVRFQHQSFT 

       430        440        450        460        470        480 
EEAKDICRLF LAKTPEQRLG SREKSDDPRK HHFFKTINFP RLEAGLVDPP FVPDPSVVYA 

       490        500        510        520        530        540 
KDVDEIEDFS EVRGVEFDDK DKQFFQRFAT GAVPIAWQEE IIETGLFEEL NDPNRPAGCG 

       550 
EGNSSRSGVC LLL 

« Hide

References

[1]"Species-specific differences in expression of G-protein-coupled receptor kinase (GRK) 7 and GRK1 in mammalian cone photoreceptor cells: implications for cone cell phototransduction."
Weiss E.R., Ducceschi M.H., Horner T.J., Li A., Craft C.M., Osawa S.
J. Neurosci. 21:9175-9184(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Retina.
[2]"Phosphorylation of GRK7 by PKA in cone photoreceptor cells is regulated by light."
Osawa S., Jo R., Weiss E.R.
J. Neurochem. 107:1314-1324(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-36.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF282270 mRNA. Translation: AAL33881.1.
RefSeqNP_999212.1. NM_214047.1.
UniGeneSsc.165.

3D structure databases

ProteinModelPortalQ8WP15.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000012443.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000012780; ENSSSCP00000012443; ENSSSCG00000011675.
GeneID397114.
KEGGssc:397114.

Organism-specific databases

CTD131890.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00730000110751.
HOGENOMHOG000006742.
HOVERGENHBG004532.
KOK00909.
OMAAYAFESK.
OrthoDBEOG7V1FQK.
TreeFamTF313940.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR00717. GPCRKINASE.
SMARTSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGRK7_PIG
AccessionPrimary (citable) accession number: Q8WP15
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families