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Protein

Steroid 21-hydroxylase

Gene

CYP21

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.By similarity

Catalytic activityi

A C(21) steroid + [reduced NADPH--hemoprotein reductase] + O2 = a 21-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi426Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processSteroidogenesis
LigandHeme, Iron, Lipid-binding, Metal-binding, Steroid-binding

Enzyme and pathway databases

ReactomeiR-CFA-193993. Mineralocorticoid biosynthesis.
R-CFA-194002. Glucocorticoid biosynthesis.
R-CFA-211976. Endogenous sterols.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 21-hydroxylase (EC:1.14.14.16By similarity)
Alternative name(s):
21-OHase
Cytochrome P-450c21
Cytochrome P450 21
Cytochrome P450 XXI
Cytochrome P450-C21
Gene namesi
Name:CYP21
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 12

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519751 – 492Steroid 21-hydroxylaseAdd BLAST492

Proteomic databases

PaxDbiQ8WNW0.

Expressioni

Gene expression databases

BgeeiENSCAFG00000000712.

Interactioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000001018.

Structurei

3D structure databases

ProteinModelPortaliQ8WNW0.
SMRiQ8WNW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni340 – 356Steroid-bindingBy similarityAdd BLAST17

Domaini

The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00880000137854.
HOGENOMiHOG000036991.
HOVERGENiHBG106944.
InParanoidiQ8WNW0.
OMAiWRDMMDY.
OrthoDBiEOG091G074I.
TreeFamiTF105095.

Family and domain databases

InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8WNW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLGVLLLT VLAGARLLWG KWKLRGLHLP PLVPGCLHLL QPDLPLHLLG
60 70 80 90 100
LTQKLGPIYR LRLGLQDVVV LNSKRTIEEA MVRKWVDFAG RPQTPSYKLV
110 120 130 140 150
SLHHQDLSLG DYSLLWKAHK KLTRSALLLG IRSSMEPLVE QLTQEFCERM
160 170 180 190 200
RAQAGTPVAI QKEFSLLTCA IICHLTFGDK EDTLVHTFHD CVQDLMRTWE
210 220 230 240 250
HWSIQMLDII PFLRFFPNPG LWRLKRALEN RDHIVEKQLR QHKESMVAGQ
260 270 280 290 300
WRDMTDYMLQ RVGRLRAEEG CGQLLEGHVH MSVVDLFIGG TETTATTLSW
310 320 330 340 350
AVAFLLHHPE IQQRLQEELD RELGPGASGS RIPYRDPTRL PLLSATVAEV
360 370 380 390 400
LRLRPVVPLA LPHCTTRPSS ISGYDIPEGM VVIPNLQGAH LDETVWERPQ
410 420 430 440 450
EFRPDRFLVP GASPRVLAFG CGARVCLGEP LARLELLVVL AQLLRAFTLM
460 470 480 490
PAAGTLPSLR PRARCGVNLS MQPFQVQLQP RGAGVLGRGQ HP
Length:492
Mass (Da):55,366
Last modified:March 1, 2002 - v1
Checksum:i8151F79FF0484A54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB052905 Genomic DNA. Translation: BAB79541.1.
UniGeneiCfa.3607.

Genome annotation databases

EnsembliENSCAFT00000001108; ENSCAFP00000001018; ENSCAFG00000000712.

Similar proteinsi

Entry informationi

Entry nameiCP21A_CANLF
AccessioniPrimary (citable) accession number: Q8WNW0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2002
Last modified: August 30, 2017
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families