Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8WNW0

- CP21A_CANFA

UniProt

Q8WNW0 - CP21A_CANFA

Protein

Steroid 21-hydroxylase

Gene

CYP21

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.

    Catalytic activityi

    A C(21) steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 21-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi426 – 4261Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. steroid 21-monooxygenase activity Source: UniProtKB-EC
    4. steroid binding Source: UniProtKB-KW

    GO - Biological processi

    1. steroid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Lipid-binding, Metal-binding, Steroid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 21-hydroxylase (EC:1.14.99.10)
    Alternative name(s):
    21-OHase
    Cytochrome P-450c21
    Cytochrome P450 21
    Cytochrome P450 XXI
    Cytochrome P450-C21
    Gene namesi
    Name:CYP21
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Chromosome 12

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 492492Steroid 21-hydroxylasePRO_0000051975Add
    BLAST

    Proteomic databases

    PaxDbiQ8WNW0.

    Interactioni

    Protein-protein interaction databases

    STRINGi9615.ENSCAFP00000001018.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8WNW0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni340 – 35617Steroid-bindingBy similarityAdd
    BLAST

    Domaini

    The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117317.
    HOGENOMiHOG000036991.
    HOVERGENiHBG106944.
    InParanoidiQ8WNW0.
    OrthoDBiEOG7BCNBG.
    TreeFamiTF105095.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8WNW0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLLGVLLLT VLAGARLLWG KWKLRGLHLP PLVPGCLHLL QPDLPLHLLG    50
    LTQKLGPIYR LRLGLQDVVV LNSKRTIEEA MVRKWVDFAG RPQTPSYKLV 100
    SLHHQDLSLG DYSLLWKAHK KLTRSALLLG IRSSMEPLVE QLTQEFCERM 150
    RAQAGTPVAI QKEFSLLTCA IICHLTFGDK EDTLVHTFHD CVQDLMRTWE 200
    HWSIQMLDII PFLRFFPNPG LWRLKRALEN RDHIVEKQLR QHKESMVAGQ 250
    WRDMTDYMLQ RVGRLRAEEG CGQLLEGHVH MSVVDLFIGG TETTATTLSW 300
    AVAFLLHHPE IQQRLQEELD RELGPGASGS RIPYRDPTRL PLLSATVAEV 350
    LRLRPVVPLA LPHCTTRPSS ISGYDIPEGM VVIPNLQGAH LDETVWERPQ 400
    EFRPDRFLVP GASPRVLAFG CGARVCLGEP LARLELLVVL AQLLRAFTLM 450
    PAAGTLPSLR PRARCGVNLS MQPFQVQLQP RGAGVLGRGQ HP 492
    Length:492
    Mass (Da):55,366
    Last modified:March 1, 2002 - v1
    Checksum:i8151F79FF0484A54
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB052905 Genomic DNA. Translation: BAB79541.1.
    UniGeneiCfa.3607.

    Genome annotation databases

    EnsembliENSCAFT00000001108; ENSCAFP00000001018; ENSCAFG00000000712.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB052905 Genomic DNA. Translation: BAB79541.1 .
    UniGenei Cfa.3607.

    3D structure databases

    ProteinModelPortali Q8WNW0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9615.ENSCAFP00000001018.

    Proteomic databases

    PaxDbi Q8WNW0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSCAFT00000001108 ; ENSCAFP00000001018 ; ENSCAFG00000000712 .

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117317.
    HOGENOMi HOG000036991.
    HOVERGENi HBG106944.
    InParanoidi Q8WNW0.
    OrthoDBi EOG7BCNBG.
    TreeFami TF105095.

    Miscellaneous databases

    NextBioi 20818803.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of canine 21-hydroxylase gene and its polymorphic analysis as a candidate gene for congenital adrenal hyperplasia-like syndrome in Pomeranians."
      Takada K., Kitamura H., Takiguchi M., Saito M., Hashimoto A.
      Res. Vet. Sci. 73:159-163(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Beagle.
      Tissue: Adrenal gland.

    Entry informationi

    Entry nameiCP21A_CANFA
    AccessioniPrimary (citable) accession number: Q8WNW0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3