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Protein

Dehydrogenase/reductase SDR family member 4

Gene

DHRS4

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones.1 Publication

Catalytic activityi

R-CHOH-R' + NADP+ = R-CO-R' + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei170 – 1701SubstrateBy similarity
Active sitei183 – 1831Proton acceptorPROSITE-ProRule annotation1 Publication
Binding sitei187 – 1871NADP1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 6125NADP1 PublicationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • oxidation-reduction process Source: UniProtKB
  • retinal metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.184. 6170.
ReactomeiR-SSC-5365859. RA biosynthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrogenase/reductase SDR family member 4 (EC:1.1.1.184)
Alternative name(s):
NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase
Short name:
CR
Short name:
PHCR
NADPH-dependent retinol dehydrogenase/reductase
Short name:
NDRD
Peroxisomal short-chain alcohol dehydrogenase
Short name:
PSCD
Gene namesi
Name:DHRS4
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome 7

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Dehydrogenase/reductase SDR family member 4PRO_0000054649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei93 – 931N6-acetyllysine; alternateBy similarity
Modified residuei93 – 931N6-succinyllysine; alternateBy similarity
Modified residuei217 – 2171N6-acetyllysine; alternateBy similarity
Modified residuei217 – 2171N6-succinyllysine; alternateBy similarity
Modified residuei221 – 2211PhosphoserineBy similarity
Modified residuei228 – 2281N6-succinyllysineBy similarity
Modified residuei235 – 2351N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8WNV7.
PeptideAtlasiQ8WNV7.

Expressioni

Tissue specificityi

Detected in heart, kidney, liver and small intestine. Detected at lower levels in brain, lung, stomach and spleen.1 Publication

Gene expression databases

BgeeiENSSSCG00000002013.
GenevisibleiQ8WNV7. SS.

Interactioni

Subunit structurei

Homotetramer.2 Publications

Protein-protein interaction databases

DIPiDIP-29647N.
STRINGi9823.ENSSSCP00000002200.

Structurei

Secondary structure

1
279
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni30 – 334Combined sources
Beta strandi35 – 406Combined sources
Helixi44 – 5512Combined sources
Beta strandi59 – 657Combined sources
Helixi67 – 7913Combined sources
Beta strandi84 – 885Combined sources
Helixi94 – 10815Combined sources
Beta strandi113 – 1164Combined sources
Helixi127 – 1293Combined sources
Helixi132 – 14211Combined sources
Helixi144 – 15916Combined sources
Beta strandi163 – 1686Combined sources
Helixi171 – 1733Combined sources
Helixi181 – 20121Combined sources
Helixi202 – 2043Combined sources
Beta strandi206 – 2138Combined sources
Helixi222 – 2254Combined sources
Helixi228 – 23811Combined sources
Helixi246 – 2494Combined sources
Helixi250 – 2567Combined sources
Helixi259 – 2613Combined sources
Beta strandi268 – 2725Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZATX-ray1.50A/B/C/D20-279[»]
ProteinModelPortaliQ8WNV7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8WNV7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi277 – 2793Microbody targeting signal

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG105779.
InParanoidiQ8WNV7.
KOiK11147.
OMAiLWMDKAR.
OrthoDBiEOG091G0G3R.
TreeFamiTF315405.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR029511. DHRS4-like.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF198. PTHR24322:SF198. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8WNV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAAGQLLRA CSQTWKSVRM ASTGVERRKP LENKVALVTA STDGIGLAIA
60 70 80 90 100
RRLAQDGAHV VVSSRKQENV DRTVATLQGE GLSVTGTVCH VGKAEDRERL
110 120 130 140 150
VAMAVNLHGG VDILVSNAAV NPFFGNIIDA TEEVWDKILH VNVKATVLMT
160 170 180 190 200
KAVVPEMEKR GGGSVLIVSS VGAYHPFPNL GPYNVSKTAL LGLTKNLAVE
210 220 230 240 250
LAPRNIRVNC LAPGLIKTNF SQVLWMDKAR KEYMKESLRI RRLGNPEDCA
260 270
GIVSFLCSED ASYITGETVV VGGGTASRL
Length:279
Mass (Da):29,919
Last modified:October 5, 2010 - v2
Checksum:iEB7E0AC55CACBCE5
GO

Sequence cautioni

The sequence BAB78528 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT961055 Genomic DNA. No translation available.
AB062757 mRNA. Translation: BAB78528.1. Different initiation.
RefSeqiNP_999184.2. NM_214019.2.
UniGeneiSsc.86.

Genome annotation databases

EnsembliENSSSCT00000002252; ENSSSCP00000002200; ENSSSCG00000002013.
GeneIDi397082.
KEGGissc:397082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT961055 Genomic DNA. No translation available.
AB062757 mRNA. Translation: BAB78528.1. Different initiation.
RefSeqiNP_999184.2. NM_214019.2.
UniGeneiSsc.86.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZATX-ray1.50A/B/C/D20-279[»]
ProteinModelPortaliQ8WNV7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29647N.
STRINGi9823.ENSSSCP00000002200.

Proteomic databases

PaxDbiQ8WNV7.
PeptideAtlasiQ8WNV7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000002252; ENSSSCP00000002200; ENSSSCG00000002013.
GeneIDi397082.
KEGGissc:397082.

Organism-specific databases

CTDi10901.

Phylogenomic databases

eggNOGiKOG0725. Eukaryota.
COG1028. LUCA.
GeneTreeiENSGT00760000118868.
HOVERGENiHBG105779.
InParanoidiQ8WNV7.
KOiK11147.
OMAiLWMDKAR.
OrthoDBiEOG091G0G3R.
TreeFamiTF315405.

Enzyme and pathway databases

BRENDAi1.1.1.184. 6170.
ReactomeiR-SSC-5365859. RA biosynthesis pathway.

Miscellaneous databases

EvolutionaryTraceiQ8WNV7.

Gene expression databases

BgeeiENSSSCG00000002013.
GenevisibleiQ8WNV7. SS.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR029511. DHRS4-like.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF198. PTHR24322:SF198. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHRS4_PIG
AccessioniPrimary (citable) accession number: Q8WNV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 5, 2010
Last modified: September 7, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibited by kaempferol, quercetin, genistein and myristic acid.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.