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Protein

Inositol oxygenase

Gene

MIOX

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Myo-inositol + O2 = D-glucuronate + H2O.

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Temperature dependencei

Thermolabile.

Pathway: myo-inositol degradation into D-glucuronate

This protein is involved in step 1 of the subpathway that synthesizes D-glucuronate from myo-inositol.
Proteins known to be involved in this subpathway in this organism are:
  1. Inositol oxygenase (MIOX)
This subpathway is part of the pathway myo-inositol degradation into D-glucuronate, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucuronate from myo-inositol, the pathway myo-inositol degradation into D-glucuronate and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261SubstrateBy similarity
Metal bindingi95 – 951Iron 1By similarity
Metal bindingi120 – 1201Iron 1By similarity
Metal bindingi121 – 1211Iron 1By similarity
Metal bindingi121 – 1211Iron 2By similarity
Binding sitei124 – 1241SubstrateBy similarity
Metal bindingi191 – 1911Iron 2By similarity
Metal bindingi217 – 2171Iron 2By similarity
Metal bindingi250 – 2501Iron 1By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular water homeostasis Source: UniProtKB
  • inositol catabolic process Source: UniProtKB
  • inositol phosphate-mediated signaling Source: UniProtKB
  • membrane organization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.99.1. 6170.
SABIO-RKQ8WN98.
UniPathwayiUPA00111; UER00527.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol oxygenase (EC:1.13.99.1)
Alternative name(s):
Aldehyde reductase-like 6
Myo-inositol oxygenase
Short name:
MI oxygenase
Gene namesi
Name:MIOX
Synonyms:ALDRL6
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • inclusion body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282Inositol oxygenasePRO_0000079150Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiQ8WN98.

Expressioni

Tissue specificityi

Kidney specific.

Gene expression databases

GenevisibleiQ8WN98. SS.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000001029.

Structurei

3D structure databases

ProteinModelPortaliQ8WN98.
SMRiQ8WN98. Positions 25-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 843Substrate bindingBy similarity
Regioni138 – 1392Substrate bindingBy similarity
Regioni217 – 2182Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the myo-inositol oxygenase family.Curated

Phylogenomic databases

eggNOGiNOG135479.
HOGENOMiHOG000163182.
HOVERGENiHBG039556.
InParanoidiQ8WN98.
KOiK00469.

Family and domain databases

InterProiIPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. DUF706. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8WN98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDPDPSQVY RPDMDPEAAK DKGSFRNYTS GPLLDRVFRT YKLMHTWQTV
60 70 80 90 100
DFVRKKHAQF GGFSYKRMTV LEAVDMLDGL VDESDPDVDF PNSFHAFQTA
110 120 130 140 150
EGIRKAHPDK DWFHLVGLLH DLGKVLVLAG EPQWAVVGDT FPVGCRPQAS
160 170 180 190 200
VVFCDSTFQD NPDLQDPVYS TELGMYQPHC GLENALMSWG HDEYMYQMMK
210 220 230 240 250
FNKFSLPGEA FYIIRFHSFY PWHTGGDYRQ LCNEQDLAML PWVQEFNKFD
260 270 280
LYTKGSDMPD VDELRPYYQG LIDKYCPGVL CW
Length:282
Mass (Da):32,653
Last modified:March 1, 2002 - v1
Checksum:i0B9D4D54A3AE5DE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF401311 mRNA. Translation: AAL39076.1.
RefSeqiNP_999267.1. NM_214102.2.
UniGeneiSsc.15816.

Genome annotation databases

GeneIDi397189.
KEGGissc:397189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF401311 mRNA. Translation: AAL39076.1.
RefSeqiNP_999267.1. NM_214102.2.
UniGeneiSsc.15816.

3D structure databases

ProteinModelPortaliQ8WN98.
SMRiQ8WN98. Positions 25-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000001029.

Proteomic databases

PaxDbiQ8WN98.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397189.
KEGGissc:397189.

Organism-specific databases

CTDi55586.

Phylogenomic databases

eggNOGiNOG135479.
HOGENOMiHOG000163182.
HOVERGENiHBG039556.
InParanoidiQ8WN98.
KOiK00469.

Enzyme and pathway databases

UniPathwayiUPA00111; UER00527.
BRENDAi1.13.99.1. 6170.
SABIO-RKQ8WN98.

Gene expression databases

GenevisibleiQ8WN98. SS.

Family and domain databases

InterProiIPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. DUF706. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Myo-inositol oxygenase: molecular cloning and expression of a unique enzyme that oxidizes myo-inositol and d-chiro-inositol."
    Arner R.J., Prabhu K.S., Thompson J.T., Hildenbrandt G.R., Liken A.D., Reddy C.C.
    Biochem. J. 360:313-320(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Tissue: Kidney.

Entry informationi

Entry nameiMIOX_PIG
AccessioniPrimary (citable) accession number: Q8WN98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: March 1, 2002
Last modified: June 24, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.