Reviewed, UniProtKB/Swiss-Prot Q8WN98 (MIOX_PIG)

Names and origin

Protein names

Recommended name:
    Inositol oxygenase
    EC=1.13.99.1
Alternative name(s):
    Myo-inositol oxygenase
      Short name=MI oxygenase
    Aldehyde reductase-like 6

Gene names

Name:	MIOX
Synonyms:	ALDRL6

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Myo-inositol + O2 = D-glucuronate + H2O.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.
Subcellular location	Cytoplasm.
Tissue specificity	Kidney specific.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the myo-inositol oxygenase family.
Biophysicochemical properties	Temperature dependence: Thermolabile.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular water homeostasis Ref.1 Traceable author statement. Source: UniProtKB inositol catabolic process Ref.1 Inferred from direct assay. Source: UniProtKB inositol phosphate-mediated signaling Ref.1 Traceable author statement. Source: UniProtKB membrane organization Ref.1 Traceable author statement. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell inclusion body Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	inositol oxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Inositol oxygenase		PRO_0000079150
Sites
	Metal binding	95	1	Iron 1 By similarity
	Metal binding	120	1	Iron 1 By similarity
	Metal binding	121	1	Iron 1 By similarity
	Metal binding	121	1	Iron 2 By similarity
	Metal binding	191	1	Iron 2 By similarity
	Metal binding	217	1	Iron 2 By similarity
	Metal binding	250	1	Iron 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8WN98-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 0B9D4D54A3AE5DE0	FASTA	282	32,653
	10 20 30 40 50 60 MKDPDPSQVY RPDMDPEAAK DKGSFRNYTS GPLLDRVFRT YKLMHTWQTV DFVRKKHAQF 70 80 90 100 110 120 GGFSYKRMTV LEAVDMLDGL VDESDPDVDF PNSFHAFQTA EGIRKAHPDK DWFHLVGLLH 130 140 150 160 170 180 DLGKVLVLAG EPQWAVVGDT FPVGCRPQAS VVFCDSTFQD NPDLQDPVYS TELGMYQPHC 190 200 210 220 230 240 GLENALMSWG HDEYMYQMMK FNKFSLPGEA FYIIRFHSFY PWHTGGDYRQ LCNEQDLAML 250 260 270 280 PWVQEFNKFD LYTKGSDMPD VDELRPYYQG LIDKYCPGVL CW

References

[1]

"Myo-inositol oxygenase: molecular cloning and expression of a unique enzyme that oxidizes myo-inositol and d-chiro-inositol." Arner R.J., Prabhu K.S., Thompson J.T., Hildenbrandt G.R., Liken A.D., Reddy C.C. Biochem. J. 360:313-320(2001) [PubMed: 11716759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF401311 mRNA. Translation: AAL39076.1.
RefSeq	NP_999267.1.
UniGene	Ssc.15816
3D structure databases
SMR	Q8WN98. Positions 25-282.
ModBase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000001051; ENSSSCP00000001029; ENSSSCG00000000961; Sus scrofa. [Genome view]
GeneID	397189.
KEGG	ssc:397189.
Organism-specific databases
CTD	397189.
Phylogenomic databases
HOVERGEN	Q8WN98.
Enzyme and pathway databases
BRENDA	1.13.99.1. 249.
Family and domain databases
InterPro	IPR007828. DUF706. [Graphical view]
PANTHER	PTHR12588. DUF706. 1 hit.
Pfam	PF05153. DUF706. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIOX_PIG

Accession

Primary (citable) accession number: Q8WN98

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	March 1, 2002
Last modified:	November 24, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)