Q8WN98 (MIOX_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inositol oxygenase
EC=1.13.99.1

Alternative name(s):
Aldehyde reductase-like 6
Myo-inositol oxygenase
Short name=MI oxygenase

Gene names

Name:	MIOX
Synonyms:	ALDRL6

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Myo-inositol + O2 = D-glucuronate + H2O.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.
Subcellular location	Cytoplasm.
Tissue specificity	Kidney specific.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the myo-inositol oxygenase family.
Biophysicochemical properties	Temperature dependence: Thermolabile.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular membrane organization Traceable author statement Ref.1. Source: UniProtKB cellular water homeostasis Traceable author statement Ref.1. Source: UniProtKB inositol catabolic process Inferred from direct assay Ref.1. Source: UniProtKB inositol phosphate-mediated signaling Traceable author statement Ref.1. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell inclusion body Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	ferric iron binding Inferred from sequence or structural similarity. Source: UniProtKB inositol oxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Inositol oxygenase		PRO_0000079150
Regions
	Region	82 – 84	3	Substrate binding By similarity
	Region	138 – 139	2	Substrate binding By similarity
	Region	217 – 218	2	Substrate binding By similarity
Sites
	Metal binding	95	1	Iron 1 By similarity
	Metal binding	120	1	Iron 1 By similarity
	Metal binding	121	1	Iron 1 By similarity
	Metal binding	121	1	Iron 2 By similarity
	Metal binding	191	1	Iron 2 By similarity
	Metal binding	217	1	Iron 2 By similarity
	Metal binding	250	1	Iron 1 By similarity
	Binding site	26	1	Substrate By similarity
	Binding site	124	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8WN98 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 0B9D4D54A3AE5DE0	FASTA	282	32,653
	10 20 30 40 50 60 MKDPDPSQVY RPDMDPEAAK DKGSFRNYTS GPLLDRVFRT YKLMHTWQTV DFVRKKHAQF 70 80 90 100 110 120 GGFSYKRMTV LEAVDMLDGL VDESDPDVDF PNSFHAFQTA EGIRKAHPDK DWFHLVGLLH 130 140 150 160 170 180 DLGKVLVLAG EPQWAVVGDT FPVGCRPQAS VVFCDSTFQD NPDLQDPVYS TELGMYQPHC 190 200 210 220 230 240 GLENALMSWG HDEYMYQMMK FNKFSLPGEA FYIIRFHSFY PWHTGGDYRQ LCNEQDLAML 250 260 270 280 PWVQEFNKFD LYTKGSDMPD VDELRPYYQG LIDKYCPGVL CW

References

[1]

"Myo-inositol oxygenase: molecular cloning and expression of a unique enzyme that oxidizes myo-inositol and d-chiro-inositol." Arner R.J., Prabhu K.S., Thompson J.T., Hildenbrandt G.R., Liken A.D., Reddy C.C. Biochem. J. 360:313-320(2001) [PubMed: 11716759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF401311 mRNA. Translation: AAL39076.1.
RefSeq	NP_999267.1. NM_214102.1.
UniGene	Ssc.15816.
3D structure databases
ProteinModelPortal	Q8WN98.
SMR	Q8WN98. Positions 25-282.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8WN98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	397189.
KEGG	ssc:397189.
Organism-specific databases
CTD	55586.
Phylogenomic databases
GeneTree	ENSGT00390000016211.
HOVERGEN	HBG039556.
OrthoDB	EOG454902.
Family and domain databases
InterPro	IPR007828. Inositol_oxygenase. [Graphical view]
KO	K00469.
PANTHER	PTHR12588. DUF706. 1 hit.
Pfam	PF05153. DUF706. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIOX_PIG

Accession

Primary (citable) accession number: Q8WN98

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	March 1, 2002
Last modified:	November 16, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program