Reviewed, UniProtKB/Swiss-Prot Q8WMV1 (ERG24_BOVIN)

Names and origin

Protein names

Recommended name:
    Delta(14)-sterol reductase
      Short name=Delta-14-SR
    EC=1.3.1.70
Alternative name(s):
    C-14 sterol reductase
    Sterol C14-reductase
    Transmembrane 7 superfamily member 2

Gene names

Name:

TM7SF2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the conversion of lanosterol to cholesterol. Ref.1
Catalytic activity	4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Ref.1
Tissue specificity	Highly expressed in liver and brain. Ref.1
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	delta14-sterol reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Delta(14)-sterol reductase		PRO_0000244771
Regions
	Transmembrane	13 – 35	23	Potential
	Transmembrane	62 – 81	20	Potential
	Transmembrane	102 – 124	23	Potential
	Transmembrane	129 – 148	20	Potential
	Transmembrane	255 – 277	23	Potential
	Transmembrane	287 – 304	18	Potential
	Transmembrane	355 – 377	23	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8WMV1-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 30DE297CFB09568C	FASTA	418	46,751
	10 20 30 40 50 60 MAPPQGSRAP LEFGGPLGAA ALMLLLPVTM FHLLLVARSG PARLLGPPPY LPGPEELWSP 70 80 90 100 110 120 WALLLCLTWL GLQAALYLLP ARKVAEGQEL KDKSRLRYPT NGFQALVLTA LLVGLGVSAG 130 140 150 160 170 180 LPLSALPEML LPLAFAATLT AFIFSLLLYL KALLAPASAL APGGNSGNLI YDFFLGRELN 190 200 210 220 230 240 PRICSFDFKY FCELRPGLIG WVLINLALLI QEAELRGSPS LAMWLVNGFQ LLYVGDALWY 250 260 270 280 290 300 EEAVLTTMDI IHDGFGFMLA FGDLAWVPFT YSLQAQFLLY HPQPLGWPLA SFICLINAVG 310 320 330 340 350 360 YYIFRGANSQ KNTFRKNPSD PRVADLETIS TATGRRLLVS GWWGMVRHPN YLGDLIMALA 370 380 390 400 410 WSLPCGVFHL LPYFYFLYFT ALLVHREDRD ERQCRQKYGL AWHEYCRRVP YRIVPYVY

References

[1]

"Cloning and expression of sterol Delta14-reductase from bovine liver." Roberti R., Bennati A.M., Galli G., Caruso D., Maras B., Aisa C., Beccari T., Della Fazia M.A., Servillo G. Eur. J. Biochem. 269:283-290(2002) [PubMed: 11784322] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY039681 mRNA. Translation: AAK91505.1.
IPI	IPI00693954.
UniGene	Bt.9567
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8WMV1.
Genome annotation databases
Ensembl	ENSBTAT00000006683; ENSBTAP00000006683; ENSBTAG00000005069; Bos taurus. [Genome view]
Phylogenomic databases
HOVERGEN	Q8WMV1.
Enzyme and pathway databases
BRENDA	1.3.1.70. 251.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG24_BOVIN

Accession

Primary (citable) accession number: Q8WMV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 1, 2002
Last modified:	October 13, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)