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Protein

G protein-coupled receptor kinase 7

Gene

GRK7

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).By similarity

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.By similarity

Enzyme regulationi

Inhibited by phosphorylation of Ser-36.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201ATPPROSITE-ProRule annotation
Active sitei316 – 3161Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi197 – 2059ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
  3. rhodopsin kinase activity Source: UniProtKB

GO - Biological processi

  1. signal transduction Source: InterPro
  2. termination of G-protein coupled receptor signaling pathway Source: InterPro
  3. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 7 (EC:2.7.11.14By similarity, EC:2.7.11.16)
Alternative name(s):
G protein-coupled receptor kinase GRK7
Gene namesi
Name:GRK7
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Unplaced

Subcellular locationi

Membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549G protein-coupled receptor kinase 7PRO_0000274193Add
BLAST
Propeptidei550 – 5523Removed in mature formBy similarityPRO_0000274194

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361Phosphoserine; by PKABy similarity
Modified residuei549 – 5491Cysteine methyl esterSequence Analysis
Lipidationi549 – 5491S-geranylgeranyl cysteineSequence Analysis

Post-translational modificationi

Autophosphorylated in vitro at Ser-490. Phosphorylation at Ser-36 is regulated by light and activated by cAMP.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PRIDEiQ8WMV0.

Interactioni

Subunit structurei

Interacts (when prenylated) with PDE6D; this promotes release from membranes.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000029175.

Structurei

3D structure databases

ProteinModelPortaliQ8WMV0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 176121RGSPROSITE-ProRule annotationAdd
BLAST
Domaini191 – 454264Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini455 – 52066AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ8WMV0.
KOiK00909.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8WMV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDMGGLDNL IANTAYLQAR KSSDADSKEL QRRRRSLMLP GPQSCEQLRQ
60 70 80 90 100
ALATDFHSLC EQQPIGRRLF RDFLATVPAY QEARGFLEEV QSWELAEEGP
110 120 130 140 150
AKGSALQGLV TTCAAAPVPG RPHPFFSPAL VTKCQAATTD DDRASLVELA
160 170 180 190 200
KAEVMAFLQD QPFREFLASP FYDKFLQWKV FEMQPVSDKY FEEFRVLGKG
210 220 230 240 250
GFGEVCAVQV KNTGKMYACK KLDKKRLKKK NGEKMALLEK EILERVSSPF
260 270 280 290 300
IVSLAYAFES KSHLCLVMSL MNGGDLKFHI YSVGEPGLDM SRVIFYSAQI
310 320 330 340 350
TCGVLHLHSL GIVYRDMKPE NVLLDDLGNC RLSDLGLAVQ IQDGKPITQR
360 370 380 390 400
AGTNGYMAPE ILMEKASYSY PVDWFAMGCS IYEMVAGRTP FRDYKEKVSK
410 420 430 440 450
EDLKQRTLKE EVRFQHSNFT EEAKDICRLF LAKTPEQRLG SREKSDDPRK
460 470 480 490 500
HHFFKTINFP RLEAGLVEPP FVPDPSVVYA KDINEIDDFS EVRGVEFDDN
510 520 530 540 550
DKQFFQRFAT GAVPIAWQEE IIETGPFAEL NDPNRPAGCG EGSSRSGVCL

LL
Length:552
Mass (Da):62,125
Last modified:February 28, 2002 - v1
Checksum:i6112CB3B9EB0376D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY049726 mRNA. Translation: AAL06241.1.
RefSeqiNP_776757.1. NM_174332.3.
UniGeneiBt.9422.

Genome annotation databases

GeneIDi281802.
KEGGibta:281802.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY049726 mRNA. Translation: AAL06241.1.
RefSeqiNP_776757.1. NM_174332.3.
UniGeneiBt.9422.

3D structure databases

ProteinModelPortaliQ8WMV0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000029175.

Proteomic databases

PRIDEiQ8WMV0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281802.
KEGGibta:281802.

Organism-specific databases

CTDi131890.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000006742.
HOVERGENiHBG004532.
InParanoidiQ8WMV0.
KOiK00909.

Miscellaneous databases

NextBioi20805715.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Zhang K., Chen J.K.C., Baehr W.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Photoreceptor cGMP phosphodiesterase delta subunit (PDEdelta) functions as a prenyl-binding protein."
    Zhang H., Liu X.H., Zhang K., Chen C.K., Frederick J.M., Prestwich G.D., Baehr W.
    J. Biol. Chem. 279:407-413(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PDE6D.

Entry informationi

Entry nameiGRK7_BOVIN
AccessioniPrimary (citable) accession number: Q8WMV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2007
Last sequence update: February 28, 2002
Last modified: January 6, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.