Reviewed, UniProtKB/Swiss-Prot Q8W519 (MMT1_MAIZE)

Names and origin

Protein names

Recommended name:
    Methionine S-methyltransferase
    EC=2.1.1.12
Alternative name(s):
    AdoMet:Met S-methyltransferase

Gene names

Name:	MMT1
Synonyms:	MMT

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	1091 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the S-methylmethionine (SMM) biosynthesis from adenosyl-L-homocysteine (AdoMet) and methionine. SMM biosynthesis (by MMT1) and degradation (by HMT-1, HMT-2 and HMT-3) constitute the SMM cycle in plants, which is probably required to achieve short term control of AdoMet level. Also able to catalyze the selenium-methylmethionine (SeMM) from AdoMet and selenium-methionine (SeMet). May play a role in phoem sulfur transport; such function is however not essential. Ref.3
Catalytic activity	S-adenosyl-L-methionine + L-methionine = S-adenosyl-L-homocysteine + S-methyl-L-methionine.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methionine S-methyltransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1091	1091	Methionine S-methyltransferase		PRO_0000204462
Experimental info
	Sequence conflict	524 – 525	2	LI → MV in AAL33599. Ref.2
	Sequence conflict	609 – 611	3	YSD → ITS in AAL33599. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8W519-1 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 81A11D882CC4A5F8	FASTA	1,091	120,309
	10 20 30 40 50 60 MAALAGEDKD VDAFLADCTA SGDAAYGAAK AVLERLHAPA TRPAARRLLG AVRRRFAASR 70 80 90 100 110 120 AAGEDCFRTF HFRIHDVVLD PHVQGFQQMK KLTMMEIPSI FIPEDWSFTF YEGLNRHPDS 130 140 150 160 170 180 IFRDKTVAEL GCGNGWISIA LAEKWCPSKV YGLDINPRAV KIAWINLYLN ALDDDGLPIY 190 200 210 220 230 240 DGEGKTLLDR VEFYESDLLS YCRDNKIELD RIVGCIPQIL NPNPEAMSKI VTENSSEEFL 250 260 270 280 290 300 YALSNYCALQ GFVEDQFGLG LIARAVEEGI SVIKPSGIMV FNMGGRPGQG VCERLFRRRG 310 320 330 340 350 360 FRITKLWQTK IMQXADTDIS ALVEXEKNSR HRFEFFMDLV GBQPICARTA WAYMKSGGHI 370 380 390 400 410 420 SHALSVYSCQ LRQPNQVKKI FEFLKDGFHE VSSSLDLSFD DDSVAEEKIP FLAYLASFLK 430 440 450 460 470 480 ENKSNPCEPP AGCLNFRKLV AGFMKSYHHI PLTPDNVVVF PSRSVAIENA LQLFSPALAI 490 500 510 520 530 540 VDEHLTRHLP KQWLTSLAIE GRADCNHADG TVTVIEAPRQ SDLLIELIRK LQPQVVVTGM 550 560 570 580 590 600 AQFEAITSAA FENLLNVTKD VGSRLFLDIS EHLELSSLPS SNGVLKYLAG KTLPSHAAIL 610 620 630 640 650 660 CGLVKNQVYS DLEVAFAISE DAAVYKALSQ TIELLEGHTS LISQHYYGCL FHELLAFQIA 670 680 690 700 710 720 DRHPQQERQP AEVIPQQMIG FSDPAVSTLK ATEFFVPGSA ESSIIHMDLD RSFLPVPSAV 730 740 750 760 770 780 NASVFESFVR QNITDSETDV RSSIQQLVKD SYGLSAAGCA EIIYGNTSVA LFNKLVLCCM 790 800 810 820 830 840 QEQGTLLFPL GTNGHYVSAA KFVNASTVTI PTNPSSGFRI EPKVLADTLK NVSRPWVYVC 850 860 870 880 890 900 GPTINPTGFL YSDSDIRELL SVCAEYGARV VIDTSFSGLE YETDGWRQWN LAGCLSSLKR 910 920 930 940 950 960 SEPSFSVVLL GELSFALTAG GHDFGFVILG DSSLAETFHS FSSLSRPHTT LKYTFKKLLG 970 980 990 1000 1010 1020 LKNQKDQHFS DLIVEQKEEL KNRANQLIQT LESCGWEAAI GCGGISMLAK PTAYMGKAFK 1030 1040 1050 1060 1070 1080 AAGFDGELDA SNIREAILRA TGLCINSSSW TGIPGYCRFS FALERGEFER AMGCIARFKE 1090 LVLGGAQMNG A

References

[1]	"S-methylmethionine plays a major role in phloem sulfur transport and is synthesized by a novel type of methyltransferase." Bourgis F., Roje S., Nuccio M.L., Fisher D.B., Tarczynski M.C., Li C., Herschbach C., Rennenberg H., Pimenta M.J., Shen T.-L., Gage D.A., Hanson A.D. Plant Cell 11:1485-1498(1999) [PubMed: 10449582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Plant one-carbon genes." Wang H., Bohnert H. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 520-611.
[3]	"Insertional inactivation of the methionine s-methyltransferase gene eliminates the s-methylmethionine cycle and increases the methylation ratio." Kocsis M.G., Ranocha P., Gage D.A., Simon E.S., Rhodes D., Peel G.J., Mellema S., Saito K., Awazuhara M., Li C., Meeley R.B., Tarczynski M.C., Wagner C., Hanson A.D. Plant Physiol. 131:1808-1815(2003) [PubMed: 12692340] [Abstract] Cited for: PROBABLE FUNCTION OF SMM CYCLE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF144079 mRNA. Translation: AAD34585.2. AF439733 mRNA. Translation: AAL33599.1.
RefSeq	NP_001104941.1.
UniGene	Zm.648 Zm.86188
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	541786.
Organism-specific databases
Gramene	Q8W519.
Enzyme and pathway databases
BRENDA	2.1.1.12. 289.
Family and domain databases
InterPro	IPR004839. Aminotrans_I/II. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR007848. Small_mtfrase. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. PF05175. MTS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MMT1_MAIZE

Accession

Primary (citable) accession number: Q8W519
Secondary accession number(s): Q9XFH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)