Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bowman-Birk type proteinase inhibitor

Gene
N/A
Organism
Lens culinaris (Lentil) (Cicer lens)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of trypsin and of chymotrypsin.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei58 – 592Reactive bond for trypsinBy similarity
Sitei84 – 852Reactive bond for chymotrypsinBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI12.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Bowman-Birk type proteinase inhibitor
Alternative name(s):
LCTI
Trypsin/chymotrypsin inhibitor
OrganismiLens culinaris (Lentil) (Cicer lens)
Taxonomic identifieri3864 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaeLens

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence analysisAdd
BLAST
Propeptidei29 – 4214Sequence analysis2 PublicationsPRO_0000003268Add
BLAST
Chaini43 – 11068Bowman-Birk type proteinase inhibitor2 PublicationsPRO_0000003269Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 1031 Publication
Disulfide bondi51 ↔ 661 Publication
Disulfide bondi54 ↔ 991 Publication
Disulfide bondi56 ↔ 641 Publication
Disulfide bondi73 ↔ 801 Publication
Disulfide bondi77 ↔ 921 Publication
Disulfide bondi82 ↔ 901 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1
110
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi54 – 563Combined sources
Beta strandi58 – 614Combined sources
Beta strandi64 – 663Combined sources
Beta strandi80 – 834Combined sources
Beta strandi89 – 924Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AIHNMR-A43-109[»]
ProteinModelPortaliQ8W4Y8.
SMRiQ8W4Y8. Positions 43-109.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8W4Y8.

Family & Domainsi

Sequence similaritiesi

Belongs to the Bowman-Birk serine protease inhibitor family.Sequence analysis

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.10.69.10. 1 hit.
InterProiIPR000877. Prot_inh_BBI.
[Graphical view]
PfamiPF00228. Bowman-Birk_leg. 1 hit.
[Graphical view]
SMARTiSM00269. BowB. 1 hit.
[Graphical view]
SUPFAMiSSF57247. SSF57247. 1 hit.
PROSITEiPS00281. BOWMAN_BIRK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8W4Y8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLMNKKAIM KLALMLFLLG FTANVVDARF DSTSFITQVL SNGDDVKSAC
60 70 80 90 100
CDTCLCTRSQ PPTCRCVDVR ESCHSACDKC VCAYSNPPQC QCYDTHKFCY
110
KACHNSEIEE
Length:110
Mass (Da):12,266
Last modified:June 21, 2005 - v2
Checksum:i0FB31C642323D764
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271D → N in CAD12252 (PubMed:15655663).Curated
Sequence conflicti97 – 971K → N in CAD12253 (PubMed:15655663).Curated
Sequence conflicti97 – 971K → N in CAD12252 (PubMed:15655663).Curated
Sequence conflicti102 – 1021A → T in CAD12253 (PubMed:15655663).Curated
Sequence conflicti110 – 1101Missing AA sequence (PubMed:16889634).Curated

Mass spectrometryi

Molecular mass is 7446.63 Da from positions 43 - 109. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ420110 Genomic DNA. Translation: CAD12253.1.
AJ420109 Genomic DNA. Translation: CAD12252.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ420110 Genomic DNA. Translation: CAD12253.1.
AJ420109 Genomic DNA. Translation: CAD12252.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AIHNMR-A43-109[»]
ProteinModelPortaliQ8W4Y8.
SMRiQ8W4Y8. Positions 43-109.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI12.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ8W4Y8.

Family and domain databases

Gene3Di2.10.69.10. 1 hit.
InterProiIPR000877. Prot_inh_BBI.
[Graphical view]
PfamiPF00228. Bowman-Birk_leg. 1 hit.
[Graphical view]
SMARTiSM00269. BowB. 1 hit.
[Graphical view]
SUPFAMiSSF57247. SSF57247. 1 hit.
PROSITEiPS00281. BOWMAN_BIRK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Bowman-Birk inhibitors in Lens: identification and characterization of two paralogous gene classes in cultivated lentil and wild relatives."
    Sonnante G., De Paolis A., Pignone D.
    Theor. Appl. Genet. 110:596-604(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-104.
    Strain: cv. Macrosperma group1 Publication and cv. Microsperma group1 Publication.
    Tissue: Leaf1 Publication.
  2. "Complete amino acid sequence of the lentil trypsin-chymotrypsin inhibitor LCI-1.7 and a discussion of atypical binding sites of Bowman-Birk inhibitors."
    Weder J.K.P., Hinkers S.C.
    J. Agric. Food Chem. 52:4219-4226(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 43-110.
    Strain: cv. Microsperma group1 Publication.
    Tissue: Seed1 Publication.
  3. "Inhibitory properties and solution structure of a potent Bowman-Birk protease inhibitor from lentil (Lens culinaris, L) seeds."
    Ragg E.M., Galbusera V., Scarafoni A., Negri A., Tedeschi G., Consonni A., Sessa F., Duranti M.
    FEBS J. 273:4024-4039(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 43-110, FUNCTION, SUBUNIT, STRUCTURE BY NMR OF 43-109, DISULFIDE BONDS, MASS SPECTROMETRY.
    Strain: cv. Macrosperma group.
    Tissue: Seed.

Entry informationi

Entry nameiIBB_LENCU
AccessioniPrimary (citable) accession number: Q8W4Y8
Secondary accession number(s): Q8W4Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: December 9, 2015
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.