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Protein

Taxane 13-alpha-hydroxylase

Gene

CYP725A2

Organism
Taxus cuspidata (Japanese yew)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Taxa-4(20),11-dien-5-alpha-ol + NADPH + O2 = taxa-4(20),11-dien-5-alpha,13-alpha-diol + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi: taxol biosynthesis

This protein is involved in the pathway taxol biosynthesis, which is part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway taxol biosynthesis and in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi431 – 4311Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Taxol biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13398.
BRENDAi1.14.13.77. 6225.
UniPathwayiUPA00842.

Names & Taxonomyi

Protein namesi
Recommended name:
Taxane 13-alpha-hydroxylase (EC:1.14.13.77)
Alternative name(s):
Cytochrome P450 725A2
Gene namesi
Name:CYP725A2
OrganismiTaxus cuspidata (Japanese yew)
Taxonomic identifieri99806 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesTaxaceaeTaxus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485Taxane 13-alpha-hydroxylasePRO_0000052203Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ8W4T9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

KOiK12922.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8W4T9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALKQLEVS PSILFVTLAV MAGIILFFRS KRHSSVKLPP GNLGFPLVGE
60 70 80 90 100
TLQFVRSLGS STPQQFIEER MSKFGDVFKT SIIGHPTVVL CGPAGNRLVL
110 120 130 140 150
SNENKLVQMS WPSSMMKLIG EDCLGGKTGE QHRIVRAALT RFLGPQALQN
160 170 180 190 200
HFAKMSSGIQ RHINEKWKGK DEATVLPLVK DLVFSVASRL FFGITEEHLQ
210 220 230 240 250
EQLHNLLEVI LVGSFSVPLN IPGFSYHKAI QARATLADIM THLIEKRRNE
260 270 280 290 300
LRAGTASENQ DLLSVLLTFT DERGNSLADK EILDNFSMLL HGSYDSTNSP
310 320 330 340 350
LTMLIKVLAS HPESYEKVAQ EQFGILSTKM EGEEIAWKDL KEMKYSWQVV
360 370 380 390 400
QETLRMYPPI FGTFRKAITD IHYNGYTIPK GWKLLWTTYS TQTKEEYFKD
410 420 430 440 450
ADQFKPSRFE EEGKHVTPYT YLPFGGGMRV CPGWEFAKME TLLFLHHFVK
460 470 480
AFSGLKAIDP NEKLSGKPLP PLPVNGLPIK LYSRS
Length:485
Mass (Da):54,653
Last modified:March 1, 2002 - v1
Checksum:iCC0B82A430F4F3B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY056019 mRNA. Translation: AAL23619.1.

Genome annotation databases

KEGGiag:AAL23619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY056019 mRNA. Translation: AAL23619.1.

3D structure databases

ProteinModelPortaliQ8W4T9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAL23619.

Phylogenomic databases

KOiK12922.

Enzyme and pathway databases

UniPathwayiUPA00842.
BioCyciMetaCyc:MONOMER-13398.
BRENDAi1.14.13.77. 6225.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Taxol biosynthesis: taxane 13alpha-hydroxylase is a cytochrome P450-dependent monooxygenase."
    Jennewein S., Rithner C.D., Williams R.M., Croteau R.B.
    Proc. Natl. Acad. Sci. U.S.A. 98:13595-13600(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

Entry informationi

Entry nameiT13H_TAXCU
AccessioniPrimary (citable) accession number: Q8W4T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: March 1, 2002
Last modified: November 11, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.