Q8W4T9 (T13H_TAXCU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Taxane 13-alpha-hydroxylase
EC=1.14.13.77

Alternative name(s):
Cytochrome P450 725A2

Gene names

Name:

CYP725A2

Organism

Taxus cuspidata (Japanese yew)

Taxonomic identifier

99806 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Taxaceae › Taxus

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Taxa-4(20),11-dien-5-alpha-ol + NADPH + O2 = taxa-4(20),11-dien-5-alpha,13-alpha-diol + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Alkaloid biosynthesis; taxol biosynthesis.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Taxol biosynthesis
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	paclitaxel biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro taxane 13-alpha-hydroxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Taxane 13-alpha-hydroxylase		PRO_0000052203
Sites
	Metal binding	431	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8W4T9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: CC0B82A430F4F3B7	FASTA	485	54,653
	10 20 30 40 50 60 MDALKQLEVS PSILFVTLAV MAGIILFFRS KRHSSVKLPP GNLGFPLVGE TLQFVRSLGS 70 80 90 100 110 120 STPQQFIEER MSKFGDVFKT SIIGHPTVVL CGPAGNRLVL SNENKLVQMS WPSSMMKLIG 130 140 150 160 170 180 EDCLGGKTGE QHRIVRAALT RFLGPQALQN HFAKMSSGIQ RHINEKWKGK DEATVLPLVK 190 200 210 220 230 240 DLVFSVASRL FFGITEEHLQ EQLHNLLEVI LVGSFSVPLN IPGFSYHKAI QARATLADIM 250 260 270 280 290 300 THLIEKRRNE LRAGTASENQ DLLSVLLTFT DERGNSLADK EILDNFSMLL HGSYDSTNSP 310 320 330 340 350 360 LTMLIKVLAS HPESYEKVAQ EQFGILSTKM EGEEIAWKDL KEMKYSWQVV QETLRMYPPI 370 380 390 400 410 420 FGTFRKAITD IHYNGYTIPK GWKLLWTTYS TQTKEEYFKD ADQFKPSRFE EEGKHVTPYT 430 440 450 460 470 480 YLPFGGGMRV CPGWEFAKME TLLFLHHFVK AFSGLKAIDP NEKLSGKPLP PLPVNGLPIK LYSRS

References

[1]	"Taxol biosynthesis: taxane 13alpha-hydroxylase is a cytochrome P450-dependent monooxygenase." Jennewein S., Rithner C.D., Williams R.M., Croteau R.B. Proc. Natl. Acad. Sci. U.S.A. 98:13595-13600(2001) [PubMed: 11707604] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY056019 mRNA. Translation: AAL23619.1.
3D structure databases
ProteinModelPortal	Q8W4T9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13398.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T13H_TAXCU

Accession

Primary (citable) accession number: Q8W4T9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	March 1, 2002
Last modified:	September 21, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program