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Protein

Taxane 13-alpha-hydroxylase

Gene

CYP725A2

Organism
Taxus cuspidata (Japanese yew)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Taxa-4(20),11-dien-5-alpha-ol + NADPH + O2 = taxa-4(20),11-dien-5-alpha,13-alpha-diol + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi: taxol biosynthesis

This protein is involved in the pathway taxol biosynthesis, which is part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway taxol biosynthesis and in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi431Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processTaxol biosynthesis
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13398.
BRENDAi1.14.13.77. 6225.
UniPathwayiUPA00842.

Names & Taxonomyi

Protein namesi
Recommended name:
Taxane 13-alpha-hydroxylase (EC:1.14.13.77)
Alternative name(s):
Cytochrome P450 725A2
Gene namesi
Name:CYP725A2
OrganismiTaxus cuspidata (Japanese yew)
Taxonomic identifieri99806 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesTaxaceaeTaxus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000522031 – 485Taxane 13-alpha-hydroxylaseAdd BLAST485

Structurei

3D structure databases

ProteinModelPortaliQ8W4T9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

KOiK12922.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiView protein in InterPro
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR036396. Cyt_P450_sf.
PfamiView protein in Pfam
PF00067. p450. 1 hit.
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiView protein in PROSITE
PS00086. CYTOCHROME_P450. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8W4T9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALKQLEVS PSILFVTLAV MAGIILFFRS KRHSSVKLPP GNLGFPLVGE
60 70 80 90 100
TLQFVRSLGS STPQQFIEER MSKFGDVFKT SIIGHPTVVL CGPAGNRLVL
110 120 130 140 150
SNENKLVQMS WPSSMMKLIG EDCLGGKTGE QHRIVRAALT RFLGPQALQN
160 170 180 190 200
HFAKMSSGIQ RHINEKWKGK DEATVLPLVK DLVFSVASRL FFGITEEHLQ
210 220 230 240 250
EQLHNLLEVI LVGSFSVPLN IPGFSYHKAI QARATLADIM THLIEKRRNE
260 270 280 290 300
LRAGTASENQ DLLSVLLTFT DERGNSLADK EILDNFSMLL HGSYDSTNSP
310 320 330 340 350
LTMLIKVLAS HPESYEKVAQ EQFGILSTKM EGEEIAWKDL KEMKYSWQVV
360 370 380 390 400
QETLRMYPPI FGTFRKAITD IHYNGYTIPK GWKLLWTTYS TQTKEEYFKD
410 420 430 440 450
ADQFKPSRFE EEGKHVTPYT YLPFGGGMRV CPGWEFAKME TLLFLHHFVK
460 470 480
AFSGLKAIDP NEKLSGKPLP PLPVNGLPIK LYSRS
Length:485
Mass (Da):54,653
Last modified:March 1, 2002 - v1
Checksum:iCC0B82A430F4F3B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY056019 mRNA. Translation: AAL23619.1.

Genome annotation databases

KEGGiag:AAL23619.

Similar proteinsi

Entry informationi

Entry nameiT13H_TAXCU
AccessioniPrimary (citable) accession number: Q8W4T9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: March 1, 2002
Last modified: October 25, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families