Q8W4S4 (VHAA3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar proton ATPase a3

Alternative name(s):
V-type proton ATPase 95 kDa subunit a isoform 3
Short name=V-ATPase 95 kDa isoform a3
Vacuolar proton pump subunit a3
Vacuolar proton translocating ATPase 95 kDa subunit a isoform 3

Gene names

Name:	VHA-a3
Ordered Locus Names:	At4g39080
ORF Names:	F19H22.180

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	821 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase. Involved in vacuolar nutrient storage (e.g. accumulation and storage of nitrate) and in tolerance to some toxic ions (e.g. zinc ions sequestration in vacuoles). Ref.11
Subunit structure	The V-ATPase is a heteromultimeric enzyme By similarity.
Subcellular location	Vacuole membrane; Multi-pass membrane protein Ref.6 Ref.7 Ref.8 Ref.10.
Tissue specificity	Expressed in etiolated seedlings hypocotyls. Ref.10
Disruption phenotype	When associated with VHA-a2 disruption, day-length-dependent growth retardation associated with a reduced accumulation and storage of nitrate ions in vacuoles. Increased sensitivity to zinc ions due to a lower zinc ions sequestration in vacuoles. Reduced calcium content. No effect on sensitivity to sodium ions. Ref.10 Ref.11
Sequence similarities	Belongs to the V-ATPase 116 kDa subunit family.
Sequence caution	The sequence CAB38828.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB80571.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Membrane Vacuole
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro ATP synthesis coupled proton transport Inferred from direct assay Ref.11. Source: TAIR cellular response to nutrient levels Inferred from genetic interaction Ref.11. Source: TAIR sequestering of zinc ion Inferred from mutant phenotype Ref.11. Source: UniProtKB vacuolar proton-transporting V-type ATPase complex assembly Inferred from mutant phenotype Ref.11. Source: UniProtKB vacuolar sequestering Inferred from mutant phenotype Ref.11. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 22430844. Source: TAIR chloroplast envelope Inferred from direct assay PubMed 20061580. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plant-type vacuole membrane Inferred from direct assay Ref.7. Source: TAIR plasma membrane Inferred from direct assay PubMed 17644812. Source: TAIR vacuolar proton-transporting V-type ATPase, V0 domain Inferred from electronic annotation. Source: InterPro
Molecular_function	hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: InterPro hydrogen-translocating pyrophosphatase activity Inferred from direct assay Ref.11. Source: TAIR nutrient reservoir activity Inferred from mutant phenotype Ref.11. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 821	821	Vacuolar proton ATPase a3		PRO_0000419781
Regions
	Topological domain	1 – 421	421	Cytoplasmic Potential
	Transmembrane	422 – 442	21	Helical; Potential
	Topological domain	443 – 469	27	Vacuolar Potential
	Transmembrane	470 – 490	21	Helical; Potential
	Topological domain	491 – 548	58	Cytoplasmic Potential
	Transmembrane	549 – 569	21	Helical; Potential
	Topological domain	570 – 581	12	Vacuolar Potential
	Transmembrane	582 – 602	21	Helical; Potential
	Topological domain	603 – 640	38	Cytoplasmic Potential
	Transmembrane	641 – 661	21	Helical; Potential
	Topological domain	662 – 758	97	Vacuolar Potential
	Transmembrane	759 – 779	21	Helical; Potential
	Topological domain	780 – 821	42	Cytoplasmic Potential
	Coiled coil	97 – 144	48	Potential
Experimental info
	Sequence conflict	512 – 513	2	AT → VP in CAA82406. Ref.5
	Sequence conflict	728	1	L → M in BAF02018. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8W4S4 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 576EDE3C6545EA49	FASTA	821	92,833
	10 20 30 40 50 60 MAESGGGGGC CPPMDLMRSE TMQLVQLIVP MESAHLTVSY LGDLGLVQFK DLNSEKSPFQ 70 80 90 100 110 120 RTYAAQIKRC GEMARKIRFF RDQMSKAGVP AKEMQGKEND IDLDDVEVKL GELEAELVEI 130 140 150 160 170 180 NANNDKLQRS YNELMEYKLV LQKAGEFFSS AHRSAADQQR ETESQQAGED LLESPLLQEE 190 200 210 220 230 240 KSIDSTKQVK LGFLTGLVPR EKSMVFERIL FRATRGNIFI RQTVIEEPVI DPNSGEKAEK 250 260 270 280 290 300 NVFVVFYSGE RAKSKILKIC EAFGANRYPF SEDLGRQAQM ITEVSGRLSE LKTTIDAGLG 310 320 330 340 350 360 QRNILLQTIG DKFELWNLKV RKEKAIYHTL NMLSLDVTKK CLVAEGWSPV FASREIQDAL 370 380 390 400 410 420 QRAAVDSNSQ VGSIFQVLRT KESPPTYFRT NKFTSAIQEI VDAYGVAKYQ EANPGVFTIV 430 440 450 460 470 480 TFPFLFAVMF GDWGHGICIL LATMYLILKE KKLASQKLGD IMEMAFGGRY VILMMSLFSI 490 500 510 520 530 540 YTGLIYNEFF SIPFPLFAPS AYDCRDVSCS EATTIGLIKV RDTYPFGLDP VWHGSRSELP 550 560 570 580 590 600 FLNSLKMKMS ILLGVSQMNL GIIMSYFNAR FFKSSVNIWF QFIPQMIFLN SLFGYLSVLI 610 620 630 640 650 660 IIKWCTGSQA DLYHVMIYMF LSPMDELGEN QLFPHQKTLQ LVLLFLALVS VPCMLLPKPF 670 680 690 700 710 720 ILKKQHEARH QGQAYAPLDE TDESLHVETN GGGSHGHEEF EFSEIFVHQL IHTIEFVLGA 730 740 750 760 770 780 VSNTASYLRL WALSLAHSEL SSVFYEKVLL LAWGYNNPLI LIVGVLVFIF ATVGVLLVME 790 800 810 820 TLSAFLHALR LHWVEFQNKF YEGDGYKFAP FTFIFTANED E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The Arabidopsis thaliana transcribed genome: the GDR cDNA program." Desprez T., Amselem J., Chiapello H., Caboche M., Hoefte H. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 466-569. Strain: cv. Columbia. Tissue: Seedling.
[6]	"Isolation of intact vacuoles and proteomic analysis of tonoplast from suspension-cultured cells of Arabidopsis thaliana." Shimaoka T., Ohnishi M., Sazuka T., Mitsuhashi N., Hara-Nishimura I., Shimazaki K., Maeshima M., Yokota A., Tomizawa K., Mimura T. Plant Cell Physiol. 45:672-683(2004) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Vacuolar H(+)-ATPase activity is required for endocytic and secretory trafficking in Arabidopsis." Dettmer J., Hong-Hermesdorf A., Stierhof Y.-D., Schumacher K. Plant Cell 18:715-730(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, GENE FAMILY.
[8]	"Identification of a vacuolar sucrose transporter in barley and Arabidopsis mesophyll cells by a tonoplast proteomic approach." Endler A., Meyer S., Schelbert S., Schneider T., Weschke W., Peters S.W., Keller F., Baginsky S., Martinoia E., Schmidt U.G. Plant Physiol. 141:196-207(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[9]	"Mapping the Arabidopsis organelle proteome." Dunkley T.P.J., Hester S., Shadforth I.P., Runions J., Weimar T., Hanton S.L., Griffin J.L., Bessant C., Brandizzi F., Hawes C., Watson R.B., Dupree P., Lilley K.S. Proc. Natl. Acad. Sci. U.S.A. 103:6518-6523(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Reduced V-ATPase activity in the trans-Golgi network causes oxylipin-dependent hypocotyl growth Inhibition in Arabidopsis." Bruex A., Liu T.-Y., Krebs M., Stierhof Y.-D., Lohmann J.U., Miersch O., Wasternack C., Schumacher K. Plant Cell 20:1088-1100(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: cv. Columbia.
[11]	"Arabidopsis V-ATPase activity at the tonoplast is required for efficient nutrient storage but not for sodium accumulation." Krebs M., Beyhl D., Goerlich E., Al-Rasheid K.A.S., Marten I., Stierhof Y.-D., Hedrich R., Schumacher K. Proc. Natl. Acad. Sci. U.S.A. 107:3251-3256(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL035679 Genomic DNA. Translation: CAB38828.1. Sequence problems. AL161594 Genomic DNA. Translation: CAB80571.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE87015.1. AY060557 mRNA. Translation: AAL31187.1. BT002615 mRNA. Translation: AAO11531.1. AK221976 mRNA. Translation: BAD94513.1. AK227321 mRNA. Translation: BAE99335.1. AK230210 mRNA. Translation: BAF02018.1. Z29167 mRNA. Translation: CAA82406.1.
IPI	IPI00525158. IPI00526614. IPI00543595.
PIR	T06068.
RefSeq	NP_568051.1. NM_120068.4.
UniGene	At.10101. At.70994.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT4G39080.1-P.
Proteomic databases
PRIDE	Q8W4S4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G39080.1; AT4G39080.1; AT4G39080.
GeneID	830063.
KEGG	ath:AT4G39080.
Organism-specific databases
TAIR	At4g39080.
Phylogenomic databases
eggNOG	COG1269.
HOGENOM	HOG000037059.
InParanoid	Q8W4S4.
KO	K02154.
OMA	GTQNFGG.
PhylomeDB	Q8W4S4.
ProtClustDB	CLSN2683585.
Gene expression databases
ArrayExpress	Q8W4S4.
Genevestigator	Q8W4S4.
Family and domain databases
InterPro	IPR002490. V-ATPase_116kDa_su. IPR026028. V-type_ATPase_116kDa_su_euka. [Graphical view]
PANTHER	PTHR11629. PTHR11629. 1 hit.
Pfam	PF01496. V_ATPase_I. 1 hit. [Graphical view]
PIRSF	PIRSF001293. ATP6V0A1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

VHAA3_ARATH

Accession

Primary (citable) accession number: Q8W4S4
Secondary accession number(s): Q0WLI9 , Q42216, Q56WQ9, Q9SVI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2012
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program