Cyclin-dependent kinase C-2 - Arabidopsis thaliana (Mouse-ear cress)

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Q8W4P1 (CDKC2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 77. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase C-2
Short name=CDKC;2
EC=2.7.11.22
EC=2.7.11.23

Gene names

Name:	CDKC-2
Ordered Locus Names:	At5g64960
ORF Names:	MXK3.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subunit structure	Interacts with CYCT1-3. Ref.5
Tissue specificity	Highly expressed in flowers. Expressed in seedlings, roots, rosettes and stems. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carpel development Inferred from genetic interaction PubMed 17468259. Source: TAIR cell cycle Inferred from electronic annotation. Source: GOC leaf development Inferred from genetic interaction PubMed 17468259. Source: TAIR mRNA processing Inferred from expression pattern PubMed 18208522. Source: TAIR regulation of viral reproduction Inferred from mutant phenotype PubMed 17468259. Source: TAIR response to virus Inferred from expression pattern PubMed 17468259. Source: TAIR
Cellular_component	cytosol Inferred from direct assay PubMed 18433157. Source: TAIR nuclear body Inferred from direct assay PubMed 18208522. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC kinase activity Inferred from direct assay PubMed 17468259. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CYCT1-3	Q8LBC0	2	EBI-2025894,EBI-2025764

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q8W4P1-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 513

513

Cyclin-dependent kinase C-2

PRO_0000293118

Regions

Domain

26 – 325

300

Protein kinase

Nucleotide binding

32 – 40

ATP By similarity

Compositional bias

2 – 5

Poly-Ala

Compositional bias

413 – 503

Gly-rich

Sites

Active site

164

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

Phosphothreonine By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

198

Phosphothreonine By similarity

Experimental info

Sequence conflict

F → Y in AAL32527. Ref.3

Sequence conflict

F → Y in AAO00925. Ref.3

Sequence conflict

188

S → P in AAL32527. Ref.3

Sequence conflict

188

S → P in AAO00925. Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: F348F40377CA3756
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FASTA

513

57,129

        10         20         30         40         50         60 
MAAAAFGQLN LEEPPPIWGS RSVDCFEKLE QIGEGTYGQV YMAKEIKTGE IVALKKIRMD 

        70         80         90        100        110        120 
NEREGFPITA IREIKILKKL HHENVIHLKE IVTSPGRDRD DQGKPDNNKY KGGIYMVFEY 

       130        140        150        160        170        180 
MDHDLTGLAD RPGLRFTVPQ IKCYMKQLLT GLHYCHVNQV LHRDIKGSNL LIDNEGNLKL 

       190        200        210        220        230        240 
ADFGLARSYS HDHTGNLTNR VITLWYRPPE LLLGATKYGP AIDMWSVGCI FAELLNGKPI 

       250        260        270        280        290        300 
LPGKTENEQL NKIYELCGSP DESNWPGVSK MPWYNQMKSS RPLKRRVREI YRHFDRHALE 

       310        320        330        340        350        360 
LLEKMLVLDP SQRICAKDAL DAEYFWTDPL PCDPKSLPTY ESSHEFQTKK KRQQMRHNEE 

       370        380        390        400        410        420 
AAKKQKLQHP QQQHSRLPPQ QHGVGQSHAA PLWPAGPNHP MNNNAPPPQI PAGGHYYGGK 

       430        440        450        460        470        480 
PRGGAPVPNR YPPSGNQTGG YNNQSRGGYS SGAYPPQGRG APYGAGPRGP SGGYGVGPPN 

       490        500        510 
YSQGGGQYGG SGGSGRGQNP MGGARNQQYG WQP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Genome-wide analysis of core cell cycle genes in Arabidopsis." Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D. Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[5]	"Novel complexes of cyclin-dependent kinases and a cyclin-like protein from Arabidopsis thaliana with a function unrelated to cell division." Barroco R.M., de Veylder L., Magyar Z., Engler G., Inze D., Mironov V. Cell. Mol. Life Sci. 60:401-412(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH CYCT1-3.
[6]	"Cell cycle regulation in plant development." Inze D., de Veylder L. Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB019236 Genomic DNA. Translation: BAA97308.1. CP002688 Genomic DNA. Translation: AED97975.1. AF360134 mRNA. Translation: AAK25844.1. AY039990 mRNA. Translation: AAK64067.1. AY062449 mRNA. Translation: AAL32527.1. BT002565 mRNA. Translation: AAO00925.1.
IPI	IPI00527247.
RefSeq	NP_201301.1. NM_125895.4.
UniGene	At.72847.
3D structure databases
HSSP	HSSP built from PDB template 1H00 based on UniProtKB P24941.
ProteinModelPortal	Q8W4P1.
SMR	Q8W4P1. Positions 23-351.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8W4P1. 12 interactions.
Proteomic databases
PaxDb	Q8W4P1.
PRIDE	Q8W4P1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G64960.1; AT5G64960.1; AT5G64960.
GeneID	836620.
KEGG	ath:AT5G64960.
Organism-specific databases
GeneFarm	3284. 108.
TAIR	At5g64960.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
InParanoid	Q8W4P1.
OMA	HHENVIH.
PhylomeDB	Q8W4P1.
ProtClustDB	CLSN2692317.
Gene expression databases
ArrayExpress	Q8W4P1.
Genevestigator	Q8W4P1.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDKC2_ARATH

Accession

Primary (citable) accession number: Q8W4P1
Secondary accession number(s): Q9LV82

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents