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Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

UBP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Is involved in resistance to the arginine analog canavanine (CAN).1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei240NucleophilePROSITE-ProRule annotation1
Active sitei907Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri95 – 166UBP-typePROSITE-ProRule annotationAdd BLAST72

GO - Molecular functioni

GO - Biological processi

  • protein deubiquitination Source: InterPro
  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT1G04860-MONOMER.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.
R-ATH-5696395. Formation of Incision Complex in GG-NER.

Protein family/group databases

MEROPSiC19.090.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 2
Short name:
AtUBP2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
Gene namesi
Name:UBP2
Ordered Locus Names:At1g04860
ORF Names:F13M7.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRilocus:2010622. AT1G04860.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • proteasome complex Source: TAIR

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi240C → A or S: Confers CAN sensitivity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806931 – 961Ubiquitin carboxyl-terminal hydrolase 2Add BLAST961

Proteomic databases

PaxDbiQ8W4N3.
PRIDEiQ8W4N3.

PTM databases

iPTMnetiQ8W4N3.

Expressioni

Gene expression databases

ExpressionAtlasiQ8W4N3. baseline and differential.
GenevisibleiQ8W4N3. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G04860.1.

Structurei

3D structure databases

ProteinModelPortaliQ8W4N3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini231 – 957USPAdd BLAST727

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri95 – 166UBP-typePROSITE-ProRule annotationAdd BLAST72

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1873. Eukaryota.
COG5560. LUCA.
HOGENOMiHOG000090316.
InParanoidiQ8W4N3.
KOiK11844.
OMAiQHVSHAI.
OrthoDBiEOG093602U8.
PhylomeDBiQ8W4N3.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
SMARTiView protein in SMART
SM00290. ZnF_UBP. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8W4N3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKAKKKAR APTKEIQTME ISKKVSEEPP SQAGEIAEGD VKAVKETQAC
60 70 80 90 100
VHFDKALNLE KVLDKIKSSR QIKCAECNEG VYGKRGTKAK GSKGKKDFSS
110 120 130 140 150
SDPKSNNKAI WLCLECGCYV CGGVGLPNGP QSHVLRHSRV TRHRLVIQWE
160 170 180 190 200
NPQLRWCFPC QLLLPVEKED NGEKKDVLSE VVKLIKGRSL NNLASSDIED
210 220 230 240 250
QCSGSGSITS DIKLEGAVTS DIEARDGYVV RGLVNLGNTC FFNSIMQNLL
260 270 280 290 300
SLDRLRDHFL KENGSGVGGP LASSLRKLFT ETKPEAGLKS VINPRAFFGS
310 320 330 340 350
FCSKAPQFRG YDQHDSHELL RCLLDSLSTE ESALRKKRGV SDNDEKSTTL
360 370 380 390 400
IESVFGGETS SIVSCMECGH SSKVYEPFLD LSLPVPFKKS PPKKPQPVSR
410 420 430 440 450
AKKAKLPPKR VPKNVSKVSK VSKVLPGMVL SELNSSGKSM AVTADSDTSC
460 470 480 490 500
SSLAPLDNGP VLETPSVLTL DNNQASESAS QSDTGFDGSW LDFIGPETSG
510 520 530 540 550
DETNLDMQED GIDNVITAEV NQIVPSPNIV ANSSVSSGDQ TLEGNTERLM
560 570 580 590 600
QDYEEIAKAE ANLDEKDVQA MQSDECPATS GISAEFSQAS CIGCDPGIGE
610 620 630 640 650
SSSSVNPWDE EELPLVVADS QILYMPYKEI SCNDKSVEGE CEASSSFVTG
660 670 680 690 700
DHEPQNSDFV DFGGLFDEPE TTEGPVFGPP SKAEASGVGF MAFSSESDPE
710 720 730 740 750
EIDDSDLPVS VERCLGHFTK HEILSDDNAW NCENCSKNLK LQRLREKRKS
760 770 780 790 800
NEDESRSSNT SNGWVKENED EGFGETEILA VKQDPNDTSC VKDHSSDGRK
810 820 830 840 850
AARIHSADES ESKGTQDEDE DSEKVITVKR DATKKVLINK APPVLTIHLK
860 870 880 890 900
RFSQDLRGRL SKLNGHVAFK EVIDLRQYMD SRCSGEDPPV YRLAGLVEHS
910 920 930 940 950
GTMRGGHYVA YVRGGQRVKE TDSSSTAWYN VSDAYVRQVS LEKVLHSEAY
960
ILFYERIFSQ E
Length:961
Mass (Da):105,152
Last modified:April 26, 2005 - v2
Checksum:iE4881E31F1ADE2C7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti261K → E in AAL32541 (PubMed:14593172).Curated1
Sequence conflicti653E → K in AAL32541 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302659 mRNA. Translation: AAG42750.1.
AC004809 Genomic DNA. Translation: AAF40451.1.
CP002684 Genomic DNA. Translation: AEE27752.1.
AY062463 mRNA. Translation: AAL32541.1.
PIRiH86181.
RefSeqiNP_563719.1. NM_100364.4.
UniGeneiAt.22815.

Genome annotation databases

EnsemblPlantsiAT1G04860.1; AT1G04860.1; AT1G04860.
GeneIDi839397.
GrameneiAT1G04860.1; AT1G04860.1; AT1G04860.
KEGGiath:AT1G04860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302659 mRNA. Translation: AAG42750.1.
AC004809 Genomic DNA. Translation: AAF40451.1.
CP002684 Genomic DNA. Translation: AEE27752.1.
AY062463 mRNA. Translation: AAL32541.1.
PIRiH86181.
RefSeqiNP_563719.1. NM_100364.4.
UniGeneiAt.22815.

3D structure databases

ProteinModelPortaliQ8W4N3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G04860.1.

Protein family/group databases

MEROPSiC19.090.

PTM databases

iPTMnetiQ8W4N3.

Proteomic databases

PaxDbiQ8W4N3.
PRIDEiQ8W4N3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G04860.1; AT1G04860.1; AT1G04860.
GeneIDi839397.
GrameneiAT1G04860.1; AT1G04860.1; AT1G04860.
KEGGiath:AT1G04860.

Organism-specific databases

AraportiAT1G04860.
TAIRilocus:2010622. AT1G04860.

Phylogenomic databases

eggNOGiKOG1873. Eukaryota.
COG5560. LUCA.
HOGENOMiHOG000090316.
InParanoidiQ8W4N3.
KOiK11844.
OMAiQHVSHAI.
OrthoDBiEOG093602U8.
PhylomeDBiQ8W4N3.

Enzyme and pathway databases

BioCyciARA:AT1G04860-MONOMER.
ReactomeiR-ATH-5689880. Ub-specific processing proteases.
R-ATH-5696395. Formation of Incision Complex in GG-NER.

Miscellaneous databases

PROiPR:Q8W4N3.

Gene expression databases

ExpressionAtlasiQ8W4N3. baseline and differential.
GenevisibleiQ8W4N3. AT.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
SMARTiView protein in SMART
SM00290. ZnF_UBP. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUBP2_ARATH
AccessioniPrimary (citable) accession number: Q8W4N3
Secondary accession number(s): Q9MAT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: June 7, 2017
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.