Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8W4N3

- UBP2_ARATH

UniProt

Q8W4N3 - UBP2_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

UBP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Is involved in resistance to the arginine analog canavanine (CAN).1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei240 – 2401NucleophilePROSITE-ProRule annotation
Active sitei907 – 9071Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri95 – 16672UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT1G04860-MONOMER.

Protein family/group databases

MEROPSiC19.090.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 2
Short name:
AtUBP2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
Gene namesi
Name:UBP2
Ordered Locus Names:At1g04860
ORF Names:F13M7.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G04860.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. proteasome complex Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi240 – 2401C → A or S: Confers CAN sensitivity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 961961Ubiquitin carboxyl-terminal hydrolase 2PRO_0000080693Add
BLAST

Proteomic databases

PaxDbiQ8W4N3.
PRIDEiQ8W4N3.

Expressioni

Gene expression databases

GenevestigatoriQ8W4N3.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G04860.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8W4N3.
SMRiQ8W4N3. Positions 106-389, 754-954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini231 – 957727USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri95 – 16672UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000090316.
InParanoidiQ8W4N3.
KOiK11844.
OMAiWDEEELP.
PhylomeDBiQ8W4N3.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8W4N3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKKAKKKAR APTKEIQTME ISKKVSEEPP SQAGEIAEGD VKAVKETQAC
60 70 80 90 100
VHFDKALNLE KVLDKIKSSR QIKCAECNEG VYGKRGTKAK GSKGKKDFSS
110 120 130 140 150
SDPKSNNKAI WLCLECGCYV CGGVGLPNGP QSHVLRHSRV TRHRLVIQWE
160 170 180 190 200
NPQLRWCFPC QLLLPVEKED NGEKKDVLSE VVKLIKGRSL NNLASSDIED
210 220 230 240 250
QCSGSGSITS DIKLEGAVTS DIEARDGYVV RGLVNLGNTC FFNSIMQNLL
260 270 280 290 300
SLDRLRDHFL KENGSGVGGP LASSLRKLFT ETKPEAGLKS VINPRAFFGS
310 320 330 340 350
FCSKAPQFRG YDQHDSHELL RCLLDSLSTE ESALRKKRGV SDNDEKSTTL
360 370 380 390 400
IESVFGGETS SIVSCMECGH SSKVYEPFLD LSLPVPFKKS PPKKPQPVSR
410 420 430 440 450
AKKAKLPPKR VPKNVSKVSK VSKVLPGMVL SELNSSGKSM AVTADSDTSC
460 470 480 490 500
SSLAPLDNGP VLETPSVLTL DNNQASESAS QSDTGFDGSW LDFIGPETSG
510 520 530 540 550
DETNLDMQED GIDNVITAEV NQIVPSPNIV ANSSVSSGDQ TLEGNTERLM
560 570 580 590 600
QDYEEIAKAE ANLDEKDVQA MQSDECPATS GISAEFSQAS CIGCDPGIGE
610 620 630 640 650
SSSSVNPWDE EELPLVVADS QILYMPYKEI SCNDKSVEGE CEASSSFVTG
660 670 680 690 700
DHEPQNSDFV DFGGLFDEPE TTEGPVFGPP SKAEASGVGF MAFSSESDPE
710 720 730 740 750
EIDDSDLPVS VERCLGHFTK HEILSDDNAW NCENCSKNLK LQRLREKRKS
760 770 780 790 800
NEDESRSSNT SNGWVKENED EGFGETEILA VKQDPNDTSC VKDHSSDGRK
810 820 830 840 850
AARIHSADES ESKGTQDEDE DSEKVITVKR DATKKVLINK APPVLTIHLK
860 870 880 890 900
RFSQDLRGRL SKLNGHVAFK EVIDLRQYMD SRCSGEDPPV YRLAGLVEHS
910 920 930 940 950
GTMRGGHYVA YVRGGQRVKE TDSSSTAWYN VSDAYVRQVS LEKVLHSEAY
960
ILFYERIFSQ E
Length:961
Mass (Da):105,152
Last modified:April 26, 2005 - v2
Checksum:iE4881E31F1ADE2C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti261 – 2611K → E in AAL32541. (PubMed:14593172)Curated
Sequence conflicti653 – 6531E → K in AAL32541. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302659 mRNA. Translation: AAG42750.1.
AC004809 Genomic DNA. Translation: AAF40451.1.
CP002684 Genomic DNA. Translation: AEE27752.1.
AY062463 mRNA. Translation: AAL32541.1.
PIRiH86181.
RefSeqiNP_563719.1. NM_100364.3.
UniGeneiAt.22815.

Genome annotation databases

EnsemblPlantsiAT1G04860.1; AT1G04860.1; AT1G04860.
GeneIDi839397.
KEGGiath:AT1G04860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF302659 mRNA. Translation: AAG42750.1 .
AC004809 Genomic DNA. Translation: AAF40451.1 .
CP002684 Genomic DNA. Translation: AEE27752.1 .
AY062463 mRNA. Translation: AAL32541.1 .
PIRi H86181.
RefSeqi NP_563719.1. NM_100364.3.
UniGenei At.22815.

3D structure databases

ProteinModelPortali Q8W4N3.
SMRi Q8W4N3. Positions 106-389, 754-954.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G04860.1-P.

Protein family/group databases

MEROPSi C19.090.

Proteomic databases

PaxDbi Q8W4N3.
PRIDEi Q8W4N3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G04860.1 ; AT1G04860.1 ; AT1G04860 .
GeneIDi 839397.
KEGGi ath:AT1G04860.

Organism-specific databases

TAIRi AT1G04860.

Phylogenomic databases

eggNOGi COG5533.
HOGENOMi HOG000090316.
InParanoidi Q8W4N3.
KOi K11844.
OMAi WDEEELP.
PhylomeDBi Q8W4N3.

Enzyme and pathway databases

BioCyci ARA:AT1G04860-MONOMER.

Gene expression databases

Genevestigatori Q8W4N3.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE, FUNCTION, MUTAGENESIS OF CYS-240.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiUBP2_ARATH
AccessioniPrimary (citable) accession number: Q8W4N3
Secondary accession number(s): Q9MAT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: October 1, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3