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Q8W4N3

- UBP2_ARATH

UniProt

Q8W4N3 - UBP2_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 2

Gene

UBP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Is involved in resistance to the arginine analog canavanine (CAN).1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei240 – 2401NucleophilePROSITE-ProRule annotation
    Active sitei907 – 9071Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri95 – 16672UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT1G04860-MONOMER.

    Protein family/group databases

    MEROPSiC19.090.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 2 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 2
    Short name:
    AtUBP2
    Ubiquitin thioesterase 2
    Ubiquitin-specific-processing protease 2
    Gene namesi
    Name:UBP2
    Ordered Locus Names:At1g04860
    ORF Names:F13M7.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G04860.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. proteasome complex Source: TAIR

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi240 – 2401C → A or S: Confers CAN sensitivity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 961961Ubiquitin carboxyl-terminal hydrolase 2PRO_0000080693Add
    BLAST

    Proteomic databases

    PaxDbiQ8W4N3.
    PRIDEiQ8W4N3.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8W4N3.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT1G04860.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8W4N3.
    SMRiQ8W4N3. Positions 106-389, 754-954.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini231 – 957727USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri95 – 16672UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5533.
    HOGENOMiHOG000090316.
    InParanoidiQ8W4N3.
    KOiK11844.
    OMAiWDEEELP.
    PhylomeDBiQ8W4N3.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8W4N3-1 [UniParc]FASTAAdd to Basket

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    MGKKAKKKAR APTKEIQTME ISKKVSEEPP SQAGEIAEGD VKAVKETQAC    50
    VHFDKALNLE KVLDKIKSSR QIKCAECNEG VYGKRGTKAK GSKGKKDFSS 100
    SDPKSNNKAI WLCLECGCYV CGGVGLPNGP QSHVLRHSRV TRHRLVIQWE 150
    NPQLRWCFPC QLLLPVEKED NGEKKDVLSE VVKLIKGRSL NNLASSDIED 200
    QCSGSGSITS DIKLEGAVTS DIEARDGYVV RGLVNLGNTC FFNSIMQNLL 250
    SLDRLRDHFL KENGSGVGGP LASSLRKLFT ETKPEAGLKS VINPRAFFGS 300
    FCSKAPQFRG YDQHDSHELL RCLLDSLSTE ESALRKKRGV SDNDEKSTTL 350
    IESVFGGETS SIVSCMECGH SSKVYEPFLD LSLPVPFKKS PPKKPQPVSR 400
    AKKAKLPPKR VPKNVSKVSK VSKVLPGMVL SELNSSGKSM AVTADSDTSC 450
    SSLAPLDNGP VLETPSVLTL DNNQASESAS QSDTGFDGSW LDFIGPETSG 500
    DETNLDMQED GIDNVITAEV NQIVPSPNIV ANSSVSSGDQ TLEGNTERLM 550
    QDYEEIAKAE ANLDEKDVQA MQSDECPATS GISAEFSQAS CIGCDPGIGE 600
    SSSSVNPWDE EELPLVVADS QILYMPYKEI SCNDKSVEGE CEASSSFVTG 650
    DHEPQNSDFV DFGGLFDEPE TTEGPVFGPP SKAEASGVGF MAFSSESDPE 700
    EIDDSDLPVS VERCLGHFTK HEILSDDNAW NCENCSKNLK LQRLREKRKS 750
    NEDESRSSNT SNGWVKENED EGFGETEILA VKQDPNDTSC VKDHSSDGRK 800
    AARIHSADES ESKGTQDEDE DSEKVITVKR DATKKVLINK APPVLTIHLK 850
    RFSQDLRGRL SKLNGHVAFK EVIDLRQYMD SRCSGEDPPV YRLAGLVEHS 900
    GTMRGGHYVA YVRGGQRVKE TDSSSTAWYN VSDAYVRQVS LEKVLHSEAY 950
    ILFYERIFSQ E 961
    Length:961
    Mass (Da):105,152
    Last modified:April 26, 2005 - v2
    Checksum:iE4881E31F1ADE2C7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti261 – 2611K → E in AAL32541. (PubMed:14593172)Curated
    Sequence conflicti653 – 6531E → K in AAL32541. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302659 mRNA. Translation: AAG42750.1.
    AC004809 Genomic DNA. Translation: AAF40451.1.
    CP002684 Genomic DNA. Translation: AEE27752.1.
    AY062463 mRNA. Translation: AAL32541.1.
    PIRiH86181.
    RefSeqiNP_563719.1. NM_100364.3.
    UniGeneiAt.22815.

    Genome annotation databases

    EnsemblPlantsiAT1G04860.1; AT1G04860.1; AT1G04860.
    GeneIDi839397.
    KEGGiath:AT1G04860.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF302659 mRNA. Translation: AAG42750.1 .
    AC004809 Genomic DNA. Translation: AAF40451.1 .
    CP002684 Genomic DNA. Translation: AEE27752.1 .
    AY062463 mRNA. Translation: AAL32541.1 .
    PIRi H86181.
    RefSeqi NP_563719.1. NM_100364.3.
    UniGenei At.22815.

    3D structure databases

    ProteinModelPortali Q8W4N3.
    SMRi Q8W4N3. Positions 106-389, 754-954.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G04860.1-P.

    Protein family/group databases

    MEROPSi C19.090.

    Proteomic databases

    PaxDbi Q8W4N3.
    PRIDEi Q8W4N3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G04860.1 ; AT1G04860.1 ; AT1G04860 .
    GeneIDi 839397.
    KEGGi ath:AT1G04860.

    Organism-specific databases

    TAIRi AT1G04860.

    Phylogenomic databases

    eggNOGi COG5533.
    HOGENOMi HOG000090316.
    InParanoidi Q8W4N3.
    KOi K11844.
    OMAi WDEEELP.
    PhylomeDBi Q8W4N3.

    Enzyme and pathway databases

    BioCyci ARA:AT1G04860-MONOMER.

    Gene expression databases

    Genevestigatori Q8W4N3.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE, FUNCTION, MUTAGENESIS OF CYS-240.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUBP2_ARATH
    AccessioniPrimary (citable) accession number: Q8W4N3
    Secondary accession number(s): Q9MAT4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3