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Q8W4N3 (UBP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 2

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 2
Short name=AtUBP2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2
Gene names
Name:UBP2
Ordered Locus Names:At1g04860
ORF Names:F13M7.15
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length961 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Is involved in resistance to the arginine analog canavanine (CAN). Ref.1

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 961961Ubiquitin carboxyl-terminal hydrolase 2
PRO_0000080693

Regions

Domain231 – 957727USP
Zinc finger95 – 16672UBP-type

Sites

Active site2401Nucleophile By similarity
Active site9071Proton acceptor By similarity

Experimental info

Mutagenesis2401C → A or S: Confers CAN sensitivity. Ref.1
Sequence conflict2611K → E in AAL32541. Ref.4
Sequence conflict6531E → K in AAL32541. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q8W4N3 [UniParc].

Last modified April 26, 2005. Version 2.
Checksum: E4881E31F1ADE2C7

FASTA961105,152
        10         20         30         40         50         60 
MGKKAKKKAR APTKEIQTME ISKKVSEEPP SQAGEIAEGD VKAVKETQAC VHFDKALNLE 

        70         80         90        100        110        120 
KVLDKIKSSR QIKCAECNEG VYGKRGTKAK GSKGKKDFSS SDPKSNNKAI WLCLECGCYV 

       130        140        150        160        170        180 
CGGVGLPNGP QSHVLRHSRV TRHRLVIQWE NPQLRWCFPC QLLLPVEKED NGEKKDVLSE 

       190        200        210        220        230        240 
VVKLIKGRSL NNLASSDIED QCSGSGSITS DIKLEGAVTS DIEARDGYVV RGLVNLGNTC 

       250        260        270        280        290        300 
FFNSIMQNLL SLDRLRDHFL KENGSGVGGP LASSLRKLFT ETKPEAGLKS VINPRAFFGS 

       310        320        330        340        350        360 
FCSKAPQFRG YDQHDSHELL RCLLDSLSTE ESALRKKRGV SDNDEKSTTL IESVFGGETS 

       370        380        390        400        410        420 
SIVSCMECGH SSKVYEPFLD LSLPVPFKKS PPKKPQPVSR AKKAKLPPKR VPKNVSKVSK 

       430        440        450        460        470        480 
VSKVLPGMVL SELNSSGKSM AVTADSDTSC SSLAPLDNGP VLETPSVLTL DNNQASESAS 

       490        500        510        520        530        540 
QSDTGFDGSW LDFIGPETSG DETNLDMQED GIDNVITAEV NQIVPSPNIV ANSSVSSGDQ 

       550        560        570        580        590        600 
TLEGNTERLM QDYEEIAKAE ANLDEKDVQA MQSDECPATS GISAEFSQAS CIGCDPGIGE 

       610        620        630        640        650        660 
SSSSVNPWDE EELPLVVADS QILYMPYKEI SCNDKSVEGE CEASSSFVTG DHEPQNSDFV 

       670        680        690        700        710        720 
DFGGLFDEPE TTEGPVFGPP SKAEASGVGF MAFSSESDPE EIDDSDLPVS VERCLGHFTK 

       730        740        750        760        770        780 
HEILSDDNAW NCENCSKNLK LQRLREKRKS NEDESRSSNT SNGWVKENED EGFGETEILA 

       790        800        810        820        830        840 
VKQDPNDTSC VKDHSSDGRK AARIHSADES ESKGTQDEDE DSEKVITVKR DATKKVLINK 

       850        860        870        880        890        900 
APPVLTIHLK RFSQDLRGRL SKLNGHVAFK EVIDLRQYMD SRCSGEDPPV YRLAGLVEHS 

       910        920        930        940        950        960 
GTMRGGHYVA YVRGGQRVKE TDSSSTAWYN VSDAYVRQVS LEKVLHSEAY ILFYERIFSQ 


E 

« Hide

References

« Hide 'large scale' references
[1]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE, FUNCTION, MUTAGENESIS OF CYS-240.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF302659 mRNA. Translation: AAG42750.1.
AC004809 Genomic DNA. Translation: AAF40451.1.
CP002684 Genomic DNA. Translation: AEE27752.1.
AY062463 mRNA. Translation: AAL32541.1.
PIRH86181.
RefSeqNP_563719.1. NM_100364.3.
UniGeneAt.22815.

3D structure databases

ProteinModelPortalQ8W4N3.
SMRQ8W4N3. Positions 106-389, 754-954.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G04860.1-P.

Protein family/group databases

MEROPSC19.090.

Proteomic databases

PaxDbQ8W4N3.
PRIDEQ8W4N3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G04860.1; AT1G04860.1; AT1G04860.
GeneID839397.
KEGGath:AT1G04860.

Organism-specific databases

TAIRAT1G04860.

Phylogenomic databases

eggNOGCOG5533.
HOGENOMHOG000090316.
InParanoidQ8W4N3.
KOK11844.
OMAWDEEELP.
PhylomeDBQ8W4N3.
ProtClustDBCLSN2916966.

Enzyme and pathway databases

BioCycARA:AT1G04860-MONOMER.

Gene expression databases

GenevestigatorQ8W4N3.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP2_ARATH
AccessionPrimary (citable) accession number: Q8W4N3
Secondary accession number(s): Q9MAT4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: April 16, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names