Q8W4N3 (UBP2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 2
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 2
Short name=AtUBP2
Ubiquitin thioesterase 2
Ubiquitin-specific-processing protease 2

Gene names

Name:	UBP2
Ordered Locus Names:	At1g04860
ORF Names:	F13M7.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	961 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Is involved in resistance to the arginine analog canavanine (CAN). Ref.1
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 UBP-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Traceable author statement PubMed 12663224. Source: TAIR
Cellular_component	chloroplast Inferred from direct assay PubMed 15028209. Source: TAIR proteasome complex Traceable author statement PubMed 12663224. Source: TAIR
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 961	961	Ubiquitin carboxyl-terminal hydrolase 2		PRO_0000080693
Regions
	Zinc finger	95 – 166	72	UBP-type
Sites
	Active site	240	1	Nucleophile By similarity
	Active site	907	1	Proton acceptor By similarity
Experimental info
	Mutagenesis	240	1	C → A or S: Confers CAN sensitivity. Ref.1
	Sequence conflict	261	1	K → E in AAL32541. Ref.4
	Sequence conflict	653	1	E → K in AAL32541. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q8W4N3 [UniParc]. Last modified April 26, 2005. Version 2. Checksum: E4881E31F1ADE2C7	FASTA	961	105,152
	10 20 30 40 50 60 MGKKAKKKAR APTKEIQTME ISKKVSEEPP SQAGEIAEGD VKAVKETQAC VHFDKALNLE 70 80 90 100 110 120 KVLDKIKSSR QIKCAECNEG VYGKRGTKAK GSKGKKDFSS SDPKSNNKAI WLCLECGCYV 130 140 150 160 170 180 CGGVGLPNGP QSHVLRHSRV TRHRLVIQWE NPQLRWCFPC QLLLPVEKED NGEKKDVLSE 190 200 210 220 230 240 VVKLIKGRSL NNLASSDIED QCSGSGSITS DIKLEGAVTS DIEARDGYVV RGLVNLGNTC 250 260 270 280 290 300 FFNSIMQNLL SLDRLRDHFL KENGSGVGGP LASSLRKLFT ETKPEAGLKS VINPRAFFGS 310 320 330 340 350 360 FCSKAPQFRG YDQHDSHELL RCLLDSLSTE ESALRKKRGV SDNDEKSTTL IESVFGGETS 370 380 390 400 410 420 SIVSCMECGH SSKVYEPFLD LSLPVPFKKS PPKKPQPVSR AKKAKLPPKR VPKNVSKVSK 430 440 450 460 470 480 VSKVLPGMVL SELNSSGKSM AVTADSDTSC SSLAPLDNGP VLETPSVLTL DNNQASESAS 490 500 510 520 530 540 QSDTGFDGSW LDFIGPETSG DETNLDMQED GIDNVITAEV NQIVPSPNIV ANSSVSSGDQ 550 560 570 580 590 600 TLEGNTERLM QDYEEIAKAE ANLDEKDVQA MQSDECPATS GISAEFSQAS CIGCDPGIGE 610 620 630 640 650 660 SSSSVNPWDE EELPLVVADS QILYMPYKEI SCNDKSVEGE CEASSSFVTG DHEPQNSDFV 670 680 690 700 710 720 DFGGLFDEPE TTEGPVFGPP SKAEASGVGF MAFSSESDPE EIDDSDLPVS VERCLGHFTK 730 740 750 760 770 780 HEILSDDNAW NCENCSKNLK LQRLREKRKS NEDESRSSNT SNGWVKENED EGFGETEILA 790 800 810 820 830 840 VKQDPNDTSC VKDHSSDGRK AARIHSADES ESKGTQDEDE DSEKVITVKR DATKKVLINK 850 860 870 880 890 900 APPVLTIHLK RFSQDLRGRL SKLNGHVAFK EVIDLRQYMD SRCSGEDPPV YRLAGLVEHS 910 920 930 940 950 960 GTMRGGHYVA YVRGGQRVKE TDSSSTAWYN VSDAYVRQVS LEKVLHSEAY ILFYERIFSQ E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY ORGANIZATION, NOMENCLATURE, FUNCTION, MUTAGENESIS OF CYS-240. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF302659 mRNA. Translation: AAG42750.1. AC004809 Genomic DNA. Translation: AAF40451.1. CP002684 Genomic DNA. Translation: AEE27752.1. AY062463 mRNA. Translation: AAL32541.1.
IPI	IPI00535986.
PIR	H86181.
RefSeq	NP_563719.1. NM_100364.3.
UniGene	At.22815.
3D structure databases
ProteinModelPortal	Q8W4N3.
SMR	Q8W4N3. Positions 106-389, 754-954.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G04860.1-P.
Protein family/group databases
MEROPS	C19.090.
Proteomic databases
PaxDb	Q8W4N3.
PRIDE	Q8W4N3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G04860.1; AT1G04860.1; AT1G04860.
GeneID	839397.
KEGG	ath:AT1G04860.
Organism-specific databases
TAIR	At1g04860.
Phylogenomic databases
eggNOG	COG5533.
HOGENOM	HOG000090316.
InParanoid	Q8W4N3.
KO	K11844.
OMA	TVSSTEC.
PhylomeDB	Q8W4N3.
ProtClustDB	CLSN2916966.
Gene expression databases
Genevestigator	Q8W4N3.
GermOnline	AT1G04860. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view]
Pfam	PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP2_ARATH

Accession

Primary (citable) accession number: Q8W4N3
Secondary accession number(s): Q9MAT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program