Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Disease resistance protein RPP8

Gene

RPP8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Disease resistance protein. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. The interaction with TIP (TCV-interacting protein) may be essential for the recognition of the avirulence proteins, and the triggering of the defense response. Triggers resistance to turnip crinkle virus (TCV) via a SAG101-dependent pathway.2 Publications

Miscellaneous

In cv. Columbia and cv. Landsberg erecta, RPP8 specifically recognizes the Emco5 avirulence protein from Hyaloperonospora parasitica, while it is not the case in cv. Di-17, where it confers resistance to Turnip Crinkle Virus upon recognition of the viral capsid protein.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi192 – 199ATPSequence analysis8

GO - Molecular functioni

  • ADP binding Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • nucleotide binding Source: TAIR

GO - Biological processi

  • cellular response to salicylic acid stimulus Source: UniProtKB
  • defense response Source: TAIR
  • defense response to virus Source: UniProtKB
  • plant-type hypersensitive response Source: TAIR
  • positive regulation of defense response to virus by host Source: UniProtKB
  • response to absence of light Source: UniProtKB
  • response to blue light Source: UniProtKB
  • response to light stimulus Source: TAIR
  • response to oomycetes Source: UniProtKB
  • response to other organism Source: TAIR
  • response to salicylic acid Source: UniProtKB
  • response to wounding Source: UniProtKB
  • signal transduction Source: GO_Central

Keywordsi

Biological processHypersensitive response, Plant defense
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Disease resistance protein RPP8
Alternative name(s):
Resistance to Peronospora parasitica protein 8
Gene namesi
Name:RPP8
Synonyms:HRT
Ordered Locus Names:At5g43470
ORF Names:MWF20.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G43470.
TAIRilocus:2176486. AT5G43470.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi418D → N in rpp8-1; loss of function. 1 Publication1
Mutagenesisi553R → K in rpp8-2; loss of function. 1 Publication1
Mutagenesisi830S → L in rpp8-1; loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002127191 – 908Disease resistance protein RPP8Add BLAST908

Proteomic databases

PaxDbiQ8W4J9.
PRIDEiQ8W4J9.

Expressioni

Tissue specificityi

Mostly expressed in leaves, and, to a lower extent, in roots.1 Publication

Inductioni

By salicylic acid (SA) (PubMed:22072959, PubMed:20831409). Induced by Hyaloperonospora arabidopsidis, benzothiadiazole (BTH) and wounding (PubMed:20831409). Degraded in darkness and in blue-light (PubMed:20624951).3 Publications

Gene expression databases

GenevisibleiQ8W4J9. AT.

Interactioni

Subunit structurei

Interacts with the NAC protein TIP (PubMed:11041886). Interacts with MORC1/CRT1 (PubMed:18191794, PubMed:20332379). Interacts with COP1 and is subsequently degraded in a 26s proteasome dependent manner (PubMed:20624951).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
COP1P432542EBI-15865035,EBI-301649

Protein-protein interaction databases

BioGridi19617. 8 interactors.
DIPiDIP-59355N.
IntActiQ8W4J9. 2 interactors.
STRINGi3702.AT5G43470.1.

Structurei

3D structure databases

ProteinModelPortaliQ8W4J9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 459NB-ARCSequence analysisAdd BLAST314
Repeati575 – 600LRR 1Sequence analysisAdd BLAST26
Repeati601 – 623LRR 2Sequence analysisAdd BLAST23
Repeati648 – 673LRR 3Sequence analysisAdd BLAST26
Repeati693 – 718LRR 4Sequence analysisAdd BLAST26
Repeati722 – 746LRR 5Sequence analysisAdd BLAST25
Repeati748 – 770LRR 6Sequence analysisAdd BLAST23
Repeati793 – 820LRR 7Sequence analysisAdd BLAST28
Repeati842 – 867LRR 8Sequence analysisAdd BLAST26
Repeati882 – 905LRR 9Sequence analysisAdd BLAST24

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili15 – 57Sequence analysisAdd BLAST43

Domaini

The LRR repeats probably act as specificity determinant of pathogen recognition.1 Publication

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG4658. Eukaryota.
COG4886. LUCA.
InParanoidiQ8W4J9.
OMAiQFINCDQ.
OrthoDBiEOG093605YG.
PhylomeDBiQ8W4J9.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.80.10.10. 1 hit.
InterProiView protein in InterPro
IPR032675. LRR_dom_sf.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR036388. WH-like_DNA-bd_sf.
PfamiView protein in Pfam
PF00931. NB-ARC. 1 hit.
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8W4J9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEAFVSFGL EKLWDLLSRE SERLQGIDGQ LDGLKRQLRS LQSLLKDADA
60 70 80 90 100
KKHGSDRVRN FLEDVKDLVF DAEDIIESYV LNKLSGKGKG VKKHVRRLAC
110 120 130 140 150
FLTDRHKVAS DIEGITKRIS EVIGEMQSFG IQQIIDGGRS LSLQERQRVQ
160 170 180 190 200
REIRQTYPDS SESDLVGVEQ SVKELVGHLV ENDVHQVVSI AGMGGIGKTT
210 220 230 240 250
LARQVFHHDL VRRHFDGFAW VCVSQQFTQK HVWQRILQEL QPHDGDILQM
260 270 280 290 300
DEYALQRKLF QLLEAGRYLV VLDDVWKKED WDVIKAVFPR KRGWKMLLTS
310 320 330 340 350
RNEGVGIHAD PTCLTFRASI LNPEESWKLC ERIVFPRRDE TEVRLDEEME
360 370 380 390 400
AMGKEMVTHC GGLPLAVKAL GGLLANKHTV PEWKRVFDNI GSQIVGGSWL
410 420 430 440 450
DDNSLNSVYR ILSLSYEDLP THLKHCFLNL AHFPEDSEIS TYSLFYYWAA
460 470 480 490 500
EGIYDGSTIE DSGEYYLEEL VRRNLVIADD NYLSWQSKYC QMHDMMREVC
510 520 530 540 550
LSKAKEENFL QIIIDPTCTS TINAQSPSRS RRLSIHSGKA FHILGHKNKT
560 570 580 590 600
KVRSLIVPRF EEDYWIRSAS VFHNLTLLRV LDLSWVKFEG GKLPCSIGGL
610 620 630 640 650
IHLRYLSLYE AKVSHLPSTM RNLKLLLYLN LRVDTEEPIH VPNVLKEMIQ
660 670 680 690 700
LRYLSLPLKM DDKTKLELGD LVNLEYLYGF STQHSSVTDL LRMTKLRYLA
710 720 730 740 750
VSLSERCNFE TLSSSLRELR NLETLNFLFS LETYMVDYMG EFVLDHFIHL
760 770 780 790 800
KQLGLAVRMS KIPDQHQFPP HLVHLFLIYC GMEEDPMPIL EKLLHLKSVR
810 820 830 840 850
LARKAFLGSR MVCSKGGFPQ LCVIEISKES ELEEWIVEEG SMPCLRTLTI
860 870 880 890 900
DDCKKLKELP DGLKYITSLK ELKIEGMKRE WKEKLVPGGE DYYKVQHIPD

VQFINCDQ
Length:908
Mass (Da):104,682
Last modified:April 11, 2003 - v2
Checksum:i1BFA35BB6B0CB5CD
GO
Isoform 2 (identifier: Q8W4J9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     294-308: WKMLLTSRNEGVGIH → ELLWYIHEALFLLNS
     309-908: Missing.

Note: Has been shown to exist only in cv. Columbia so far.
Show »
Length:308
Mass (Da):35,456
Checksum:iF6F99BB4D03F6513
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti83K → R in AAL32592 (PubMed:14593172).Curated1
Sequence conflicti686S → G in AAL32592 (PubMed:14593172).Curated1
Sequence conflicti835W → R in AAL32592 (PubMed:14593172).Curated1

Polymorphismi

The strong polymorphisms present in cv. Di-17 and cv. Columbia are probably due to an unequal crossing-over between the highly related RPP8 and RPH8A genes present in cv. Landsberg erecta. Such variations probably modify the specificity of pathogen recognition.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti27 – 31IDGQL → VDEQI in strain: cv. Landsberg erecta. 1 Publication5
Natural varianti29G → E in strain: cv. Di-17. 1 Publication1
Natural varianti85 – 87SGK → RGE in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications3
Natural varianti88 – 91GKGV → EKGI in strain: cv. Landsberg erecta. 1 Publication4
Natural varianti100C → R in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti121E → D in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti129F → L in strain: cv. Di-17. 1 Publication1
Natural varianti133Q → QQ in strain: cv. Di-17. 1 Publication1
Natural varianti138G → V in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti173K → T in strain: cv. Di-17. 1 Publication1
Natural varianti177G → C in strain: cv. Di-17. 1 Publication1
Natural varianti253Y → S in strain: cv. Di-17. 1 Publication1
Natural varianti257R → P in strain: cv. Di-17. 1 Publication1
Natural varianti265A → T in strain: cv. Di-17. 1 Publication1
Natural varianti267R → K in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti270V → L in strain: cv. Di-17. 1 Publication1
Natural varianti283V → R in strain: cv. Di-17. 1 Publication1
Natural varianti369A → V in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1
Natural varianti387F → S in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1
Natural varianti399W → C in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1
Natural varianti426C → R in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1
Natural varianti429N → F in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1
Natural varianti436 – 446DSEISTYSLFY → YSKISAYDLFN in strain: cv. Landsberg erecta. 1 PublicationAdd BLAST11
Natural varianti438 – 443EISTYS → KITTQE in strain: cv. Di-17. 1 Publication6
Natural varianti450A → V in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti460E → Q in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti480 – 486DNYLSWQ → NKYLRVH in strain: cv. Di-17. 1 Publication7
Natural varianti480 – 481DN → NR in strain: cv. Landsberg erecta. 1 Publication2
Natural varianti485 – 486WQ → SH in strain: cv. Landsberg erecta. 1 Publication2
Natural varianti489Y → N in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti514I → K in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1
Natural varianti518C → S in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1
Natural varianti519T → I in strain: cv. Di-17. 1 Publication1
Natural varianti528S → R in strain: cv. Di-17. 1 Publication1
Natural varianti537S → R in strain: cv. Di-17. 1 Publication1
Natural varianti542H → Q in strain: cv. Di-17. 1 Publication1
Natural varianti543I → L in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti547 – 550KNKT → RNNA in strain: cv. Di-17. 1 Publication4
Natural varianti549K → N in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti558 – 565PRFEEDYW → WDEDFG in strain: cv. Landsberg erecta. 1 Publication8
Natural varianti558 – 560PRF → SRFK in strain: cv. Di-17. 1 Publication3
Natural varianti564Y → F in strain: cv. Di-17. 1 Publication1
Natural varianti584S → Y in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti595C → S in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1
Natural varianti609 – 612YEAK → FLAG in strain: cv. Landsberg erecta. 1 Publication4
Natural varianti630 – 635NLRVDT → DLSVHE in strain: cv. Di-17. 1 Publication6
Natural varianti632 – 636RVDTE → SVNNK in strain: cv. Landsberg erecta. 1 Publication5
Natural varianti650Q → E in strain: cv. Di-17. 1 Publication1
Natural varianti653 – 654YL → HI in strain: cv. Di-17. 1 Publication2
Natural varianti659Missing in strain: cv. Di-17. 1 Publication1
Natural varianti660M → K in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti676 – 678YLY → FLF in strain: cv. Landsberg erecta. 1 Publication3
Natural varianti678 – 679YG → FR in strain: cv. Di-17. 1 Publication2
Natural varianti692R → H in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti697R → Q in strain: cv. Di-17. 1 Publication1
Natural varianti700A → G in strain: cv. Di-17. 1 Publication1
Natural varianti724T → S in strain: cv. Di-17. 1 Publication1
Natural varianti726 – 727NF → YV in strain: cv. Landsberg erecta. 1 Publication2
Natural varianti730 – 731SL → TP in strain: cv. Di-17. 1 Publication2
Natural varianti731 – 734LETY → PEIF in strain: cv. Landsberg erecta. 1 Publication4
Natural varianti752Q → E in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1
Natural varianti768F → L in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti773 – 781VHLFLIYCG → AQIYICNCR in strain: cv. Landsberg erecta. 1 Publication9
Natural varianti773 – 781VHLFLIYCG → THIHLLFCR in strain: cv. Di-17. 1 Publication9
Natural varianti800 – 807RLARKAFL → QLTDEAFV in strain: cv. Di-17. 1 Publication8
Natural varianti800 – 803RLAR → KLTF in strain: cv. Landsberg erecta. 1 Publication4
Natural varianti807 – 809LGS → AGR in strain: cv. Landsberg erecta. 1 Publication3
Natural varianti819P → T in strain: cv. Landsberg erecta. 1 Publication1
Natural varianti823 – 824VI → AL in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications2
Natural varianti825E → D in strain: cv. Di-17. 1 Publication1
Natural varianti828 – 829KE → EQ in strain: cv. Landsberg erecta. 1 Publication2
Natural varianti851D → H in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1
Natural varianti854K → E in strain: cv. Di-17 and cv. Landsberg erecta. 2 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_007171294 – 308WKMLL…GVGIH → ELLWYIHEALFLLNS in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_007172309 – 908Missing in isoform 2. 1 PublicationAdd BLAST600

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF089710 Genomic DNA. Translation: AAC83165.1.
AF089711 Genomic DNA. Translation: AAC78631.1.
AF234174 Genomic DNA. Translation: AAF36987.1.
AB025638 Genomic DNA. Translation: BAA97426.1.
CP002688 Genomic DNA. Translation: AED94967.1.
CP002688 Genomic DNA. Translation: AED94968.1.
CP002688 Genomic DNA. Translation: ANM71032.1.
CP002688 Genomic DNA. Translation: ANM71033.1.
AK118862 mRNA. Translation: BAC43449.1.
AY062514 mRNA. Translation: AAL32592.1.
PIRiT48898.
T48899.
RefSeqiNP_001318736.1. NM_001344485.1. [Q8W4J9-1]
NP_001332592.1. NM_001344486.1. [Q8W4J9-1]
NP_199160.1. NM_123713.4. [Q8W4J9-1]
NP_851124.1. NM_180793.1. [Q8W4J9-1]
UniGeneiAt.47555.
At.49144.
At.66702.

Genome annotation databases

EnsemblPlantsiAT5G43470.1; AT5G43470.1; AT5G43470. [Q8W4J9-1]
AT5G43470.2; AT5G43470.2; AT5G43470. [Q8W4J9-1]
AT5G43470.3; AT5G43470.3; AT5G43470. [Q8W4J9-1]
AT5G43470.4; AT5G43470.4; AT5G43470. [Q8W4J9-1]
GeneIDi834367.
GrameneiAT5G43470.1; AT5G43470.1; AT5G43470.
AT5G43470.2; AT5G43470.2; AT5G43470.
AT5G43470.3; AT5G43470.3; AT5G43470.
AT5G43470.4; AT5G43470.4; AT5G43470.
KEGGiath:AT5G43470.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRPP8_ARATH
AccessioniPrimary (citable) accession number: Q8W4J9
Secondary accession number(s): Q8GWG5
, Q9M5A1, Q9ZSY3, Q9ZSY4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 11, 2003
Last sequence update: April 11, 2003
Last modified: November 22, 2017
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families