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Protein

Protein TIFY 7

Gene

TIFY7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of jasmonate responses. Jasmonoyl-isoleucine (JA-Ile) specifically promotes COI1-TIFY7/JAZ9 interaction.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processJasmonic acid signaling pathway, Plant defense, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TIFY 7
Alternative name(s):
Jasmonate ZIM domain-containing protein 9
Gene namesi
Name:TIFY7
Synonyms:JAZ9
Ordered Locus Names:At1g70700
ORF Names:F5A18.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRilocus:2033607. AT1G70700.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi223 – 224RK → AA: Loss of interaction with COI1 and resistant to coronatine-mediated degradation. No effect on MYC2 binding and nuclear localization. 2 Publications2
Mutagenesisi223R → A: No effect. 1
Mutagenesisi224K → A: No effect. 1
Mutagenesisi233 – 235KRK → AAA: Loss of interaction with MYC2 and COI1 and loss of nuclear localization. 1 Publication3
Mutagenesisi233 – 235KRK → ARA: Sensitive to coronatine-mediated degradation and no effect on nuclear localization. 1 Publication3
Mutagenesisi233K → A: No effect on nuclear localization. 1
Mutagenesisi234R → A: Loss of interaction with COI1 and resistant to coronatine-mediated degradation. Loss of nuclear localization. 1
Mutagenesisi235K → A: No effect on interaction with MYC2 and nuclear localization. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003006491 – 267Protein TIFY 7Add BLAST267

Post-translational modificationi

Ubiquitinated. Targeted for degradation by the SCF(COI1) E3 ubiquitin ligase-proteasome pathway during jasmonate signaling.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ8W4J8.

Expressioni

Inductioni

Up-regulated by jasmonate, wounding and herbivory.2 Publications

Gene expression databases

ExpressionAtlasiQ8W4J8. baseline and differential.
GenevisibleiQ8W4J8. AT.

Interactioni

Subunit structurei

Homo- and heterodimer. Interacts with MYC2, MYC3, MYC4, COI1, AFPH2/NINJA, TIFY10A/JAZ1, TIFY10B/JAZ2, TIFY6B/JAZ3, TIFY5A/JAZ8, TIFY9/JAZ10 and TIFY3A/JAZ11.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi28627. 24 interactors.
DIPiDIP-52213N.
IntActiQ8W4J8. 9 interactors.
STRINGi3702.AT1G70700.1.

Structurei

Secondary structure

1267
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi219 – 234Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RS9X-ray1.95B218-239[»]
4YWCX-ray2.40C/D218-239[»]
SMRiQ8W4J8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini113 – 148TifyPROSITE-ProRule annotationAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi178 – 181Poly-Ser4

Domaini

The jas domain (220-244) is necessary and sufficient for interaction with COI1.2 Publications

Sequence similaritiesi

Belongs to the TIFY/JAZ family.Curated

Phylogenomic databases

eggNOGiENOG410JWAB. Eukaryota.
ENOG411054B. LUCA.
HOGENOMiHOG000015145.
InParanoidiQ8W4J8.
KOiK13464.
OMAiNAMSMIE.
OrthoDBiEOG09360N7W.

Family and domain databases

InterProiView protein in InterPro
IPR018467. CCT_CS.
IPR010399. Tify_dom.
PfamiView protein in Pfam
PF09425. CCT_2. 1 hit.
PF06200. tify. 1 hit.
SMARTiView protein in SMART
SM00979. TIFY. 1 hit.
PROSITEiView protein in PROSITE
PS51320. TIFY. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8W4J8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERDFLGLSD KQYLSNNVKH EVNDDAVEER GLSTKAAREW GKSKVFATSS
60 70 80 90 100
FMPSSDFQEA KAFPGAYQWG SVSAANVFRR CQFGGAFQNA TPLLLGGSVP
110 120 130 140 150
LPTHPSLVPR VASSGSSPQL TIFYGGTISV FNDISPDKAQ AIMLCAGNGL
160 170 180 190 200
KGETGDSKPV REAERMYGKQ IHNTAATSSS SATHTDNFSR CRDTPVAATN
210 220 230 240 250
AMSMIESFNA APRNMIPSVP QARKASLARF LEKRKERLMS AMPYKKMLLD
260
LSTGESSGMN YSSTSPT
Length:267
Mass (Da):28,842
Last modified:July 27, 2011 - v2
Checksum:i855ACCF25C47B650
GO
Isoform 2 (identifier: Q8W4J8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     167-190: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:243
Mass (Da):26,278
Checksum:iB3C45AF1D929ED4C
GO

Sequence cautioni

The sequence AAG52332 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAF01974 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133D → N in AAL32593 (PubMed:14593172).Curated1
Sequence conflicti133D → N in AAM10238 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027851167 – 190Missing in isoform 2. CuratedAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011663 Genomic DNA. Translation: AAG52332.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35101.1.
CP002684 Genomic DNA. Translation: AEE35102.1.
AY062515 mRNA. Translation: AAL32593.1.
AY081676 mRNA. Translation: AAM10238.1.
AK230165 mRNA. Translation: BAF01974.1. Different initiation.
PIRiD96731.
RefSeqiNP_001031264.4. NM_001036187.5. [Q8W4J8-2]
NP_177227.5. NM_105738.8. [Q8W4J8-1]
UniGeneiAt.23705.
At.69290.

Genome annotation databases

EnsemblPlantsiAT1G70700.1; AT1G70700.1; AT1G70700. [Q8W4J8-1]
GeneIDi843407.
GrameneiAT1G70700.1; AT1G70700.1; AT1G70700.
KEGGiath:AT1G70700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011663 Genomic DNA. Translation: AAG52332.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE35101.1.
CP002684 Genomic DNA. Translation: AEE35102.1.
AY062515 mRNA. Translation: AAL32593.1.
AY081676 mRNA. Translation: AAM10238.1.
AK230165 mRNA. Translation: BAF01974.1. Different initiation.
PIRiD96731.
RefSeqiNP_001031264.4. NM_001036187.5. [Q8W4J8-2]
NP_177227.5. NM_105738.8. [Q8W4J8-1]
UniGeneiAt.23705.
At.69290.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RS9X-ray1.95B218-239[»]
4YWCX-ray2.40C/D218-239[»]
SMRiQ8W4J8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28627. 24 interactors.
DIPiDIP-52213N.
IntActiQ8W4J8. 9 interactors.
STRINGi3702.AT1G70700.1.

Proteomic databases

PaxDbiQ8W4J8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G70700.1; AT1G70700.1; AT1G70700. [Q8W4J8-1]
GeneIDi843407.
GrameneiAT1G70700.1; AT1G70700.1; AT1G70700.
KEGGiath:AT1G70700.

Organism-specific databases

AraportiAT1G70700.
TAIRilocus:2033607. AT1G70700.

Phylogenomic databases

eggNOGiENOG410JWAB. Eukaryota.
ENOG411054B. LUCA.
HOGENOMiHOG000015145.
InParanoidiQ8W4J8.
KOiK13464.
OMAiNAMSMIE.
OrthoDBiEOG09360N7W.

Miscellaneous databases

PROiPR:Q8W4J8.

Gene expression databases

ExpressionAtlasiQ8W4J8. baseline and differential.
GenevisibleiQ8W4J8. AT.

Family and domain databases

InterProiView protein in InterPro
IPR018467. CCT_CS.
IPR010399. Tify_dom.
PfamiView protein in Pfam
PF09425. CCT_2. 1 hit.
PF06200. tify. 1 hit.
SMARTiView protein in SMART
SM00979. TIFY. 1 hit.
PROSITEiView protein in PROSITE
PS51320. TIFY. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTIF7_ARATH
AccessioniPrimary (citable) accession number: Q8W4J8
Secondary accession number(s): A8MSB0
, F4I6W0, Q0WLN3, Q2V4D4, Q9CAC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 27, 2011
Last modified: April 12, 2017
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.