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Protein

Protein TIFY 7

Gene

TIFY7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Repressor of jasmonate responses. Jasmonoyl-isoleucine (JA-Ile) specifically promotes COI1-TIFY7/JAZ9 interaction.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processJasmonic acid signaling pathway, Plant defense, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein TIFY 7
Alternative name(s):
Jasmonate ZIM domain-containing protein 9
Gene namesi
Name:TIFY7
Synonyms:JAZ9
Ordered Locus Names:At1g70700
ORF Names:F5A18.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G70700
TAIRilocus:2033607 AT1G70700

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi223 – 224RK → AA: Loss of interaction with COI1 and resistant to coronatine-mediated degradation. No effect on MYC2 binding and nuclear localization. 2 Publications2
Mutagenesisi223R → A: No effect. 1
Mutagenesisi224K → A: No effect. 1
Mutagenesisi233 – 235KRK → AAA: Loss of interaction with MYC2 and COI1 and loss of nuclear localization. 1 Publication3
Mutagenesisi233 – 235KRK → ARA: Sensitive to coronatine-mediated degradation and no effect on nuclear localization. 1 Publication3
Mutagenesisi233K → A: No effect on nuclear localization. 1
Mutagenesisi234R → A: Loss of interaction with COI1 and resistant to coronatine-mediated degradation. Loss of nuclear localization. 1
Mutagenesisi235K → A: No effect on interaction with MYC2 and nuclear localization. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003006491 – 267Protein TIFY 7Add BLAST267

Post-translational modificationi

Ubiquitinated. Targeted for degradation by the SCF(COI1) E3 ubiquitin ligase-proteasome pathway during jasmonate signaling.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ8W4J8

Expressioni

Inductioni

Up-regulated by jasmonate, wounding and herbivory.2 Publications

Gene expression databases

ExpressionAtlasiQ8W4J8 baseline and differential
GenevisibleiQ8W4J8 AT

Interactioni

Subunit structurei

Homo- and heterodimer. Interacts with MYC2, MYC3, MYC4, COI1, AFPH2/NINJA, TIFY10A/JAZ1, TIFY10B/JAZ2, TIFY6B/JAZ3, TIFY5A/JAZ8, TIFY9/JAZ10 and TIFY3A/JAZ11.6 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi28627, 24 interactors
DIPiDIP-52213N
ELMiQ8W4J8
IntActiQ8W4J8, 17 interactors
STRINGi3702.AT1G70700.1

Structurei

Secondary structure

1267
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi219 – 234Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RS9X-ray1.95B218-239[»]
4YWCX-ray2.40C/D218-239[»]
SMRiQ8W4J8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini113 – 148TifyPROSITE-ProRule annotationAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi178 – 181Poly-Ser4

Domaini

The jas domain (220-244) is necessary and sufficient for interaction with COI1.2 Publications

Sequence similaritiesi

Belongs to the TIFY/JAZ family.Curated

Phylogenomic databases

eggNOGiENOG410JWAB Eukaryota
ENOG411054B LUCA
HOGENOMiHOG000015145
InParanoidiQ8W4J8
KOiK13464
OMAiKAFPGAY
OrthoDBiEOG09360N7W

Family and domain databases

InterProiView protein in InterPro
IPR018467 CCT_CS
IPR010399 Tify_dom
PfamiView protein in Pfam
PF09425 CCT_2, 1 hit
PF06200 tify, 1 hit
SMARTiView protein in SMART
SM00979 TIFY, 1 hit
PROSITEiView protein in PROSITE
PS51320 TIFY, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8W4J8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERDFLGLSD KQYLSNNVKH EVNDDAVEER GLSTKAAREW GKSKVFATSS
60 70 80 90 100
FMPSSDFQEA KAFPGAYQWG SVSAANVFRR CQFGGAFQNA TPLLLGGSVP
110 120 130 140 150
LPTHPSLVPR VASSGSSPQL TIFYGGTISV FNDISPDKAQ AIMLCAGNGL
160 170 180 190 200
KGETGDSKPV REAERMYGKQ IHNTAATSSS SATHTDNFSR CRDTPVAATN
210 220 230 240 250
AMSMIESFNA APRNMIPSVP QARKASLARF LEKRKERLMS AMPYKKMLLD
260
LSTGESSGMN YSSTSPT
Length:267
Mass (Da):28,842
Last modified:July 27, 2011 - v2
Checksum:i855ACCF25C47B650
GO
Isoform 2 (identifier: Q8W4J8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     167-190: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:243
Mass (Da):26,278
Checksum:iB3C45AF1D929ED4C
GO

Sequence cautioni

The sequence AAG52332 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAF01974 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti133D → N in AAL32593 (PubMed:14593172).Curated1
Sequence conflicti133D → N in AAM10238 (PubMed:14593172).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027851167 – 190Missing in isoform 2. CuratedAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011663 Genomic DNA Translation: AAG52332.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE35101.1
CP002684 Genomic DNA Translation: AEE35102.1
AY062515 mRNA Translation: AAL32593.1
AY081676 mRNA Translation: AAM10238.1
AK230165 mRNA Translation: BAF01974.1 Different initiation.
PIRiD96731
RefSeqiNP_001031264.4, NM_001036187.5 [Q8W4J8-2]
NP_177227.5, NM_105738.8 [Q8W4J8-1]
UniGeneiAt.23705
At.69290

Genome annotation databases

EnsemblPlantsiAT1G70700.1; AT1G70700.1; AT1G70700 [Q8W4J8-1]
AT1G70700.2; AT1G70700.2; AT1G70700 [Q8W4J8-2]
GeneIDi843407
GrameneiAT1G70700.1; AT1G70700.1; AT1G70700 [Q8W4J8-1]
AT1G70700.2; AT1G70700.2; AT1G70700 [Q8W4J8-2]
KEGGiath:AT1G70700

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTIF7_ARATH
AccessioniPrimary (citable) accession number: Q8W4J8
Secondary accession number(s): A8MSB0
, F4I6W0, Q0WLN3, Q2V4D4, Q9CAC0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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