Q8W4J2 (MPK16_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase 16
Short name=AtMPK16
Short name=MAP kinase 16
EC=2.7.11.24

Gene names

Name:	MPK16
Ordered Locus Names:	At5g19010
ORF Names:	T16G12.50

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by threonine and tyrosine phosphorylation By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR vacuole Inferred from direct assay PubMed 15539469. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from sequence or structural similarity PubMed 11544109. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Mitogen-activated protein kinase 16		PRO_0000245816
Regions
	Domain	25 – 316	292	Protein kinase
	Nucleotide binding	31 – 39	9	ATP By similarity
	Motif	187 – 189	3	TXY
Sites
	Active site	151	1	Proton acceptor By similarity
	Binding site	54	1	ATP By similarity
Amino acid modifications
	Modified residue	187	1	Phosphothreonine By similarity
	Modified residue	189	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	397	1	K → R in AAL32607. Ref.3
	Sequence conflict	397	1	K → R in AAN15447. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8W4J2 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: F6D817F558EFCC33	FASTA	567	64,912
	10 20 30 40 50 60 MQPDHRKKSS VEVDFFTEYG EGSRYRIEEV IGKGSYGVVC SAYDTHTGEK VAIKKINDIF 70 80 90 100 110 120 EHVSDATRIL REIKLLRLLR HPDIVEIKHI LLPPSRREFR DIYVVFELME SDLHQVIKAN 130 140 150 160 170 180 DDLTPEHYQF FLYQLLRGLK YIHTANVFHR DLKPKNILAN ADCKLKICDF GLARVAFNDT 190 200 210 220 230 240 PTAIFWTDYV ATRWYRAPEL CGSFFSKYTP AIDIWSIGCI FAELLTGKPL FPGKNVVHQL 250 260 270 280 290 300 DLMTDMLGTP SAEAIGRVRN EKARRYLSSM RKKKPIPFSH KFPHTDPLAL RLLEKMLSFE 310 320 330 340 350 360 PKDRPTAEEA LADVYFKGLA KVEREPSAQP VTKLEFEFER RRITKEDVRE LIYRESLEYH 370 380 390 400 410 420 PKMLKEYLDG SEPTNFMYPS AVEHFKKQFA YLEEHYKNGT SHNPPERQQH ASLPRACVLY 430 440 450 460 470 480 SDNNHPVAQQ SSAEVTDGLS KCSIRDERPR GADRNAQMPM SRIPINVPQT IQGAAVARPG 490 500 510 520 530 540 KVVGSVLRYN NCGAATGVEA LEQQQRRMVR NPAAASQYPK RTQPCKSNRG DEDCATAAEG 550 560 PSRLKPNTQY IPQKVSAAQD TAMSRWY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Mitogen-activated protein kinase cascades in plants: a new nomenclature." MAPK group Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC068809 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED92639.1. AY062529 mRNA. Translation: AAL32607.1. BT000128 mRNA. Translation: AAN15447.1.
IPI	IPI00522890.
RefSeq	NP_197402.1. NM_121906.3.
UniGene	At.19792. At.20179.
3D structure databases
ProteinModelPortal	Q8W4J2.
SMR	Q8W4J2. Positions 11-399.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8W4J2. 1 interaction.
STRING	3702.AT5G19010.1-P.
Proteomic databases
PaxDb	Q8W4J2.
PRIDE	Q8W4J2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G19010.1; AT5G19010.1; AT5G19010.
GeneID	832019.
KEGG	ath:AT5G19010.
Organism-specific databases
GeneFarm	1568. 89.
TAIR	At5g19010.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
InParanoid	Q8W4J2.
OMA	MRKKKPI.
PhylomeDB	Q8W4J2.
ProtClustDB	CLSN2686757.
Gene expression databases
Genevestigator	Q8W4J2.
GermOnline	AT5G19010. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPK16_ARATH

Accession

Primary (citable) accession number: Q8W4J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	September 5, 2006
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program