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Q8W4J2

- MPK16_ARATH

UniProt

Q8W4J2 - MPK16_ARATH

Protein

Mitogen-activated protein kinase 16

Gene

MPK16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (05 Sep 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei54 – 541ATPPROSITE-ProRule annotation
    Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: TAIR

    GO - Biological processi

    1. MAPK cascade Source: GOC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G19010-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 16 (EC:2.7.11.24)
    Short name:
    AtMPK16
    Short name:
    MAP kinase 16
    Gene namesi
    Name:MPK16
    Ordered Locus Names:At5g19010
    ORF Names:T16G12.50
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G19010.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: TAIR
    2. vacuole Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 567567Mitogen-activated protein kinase 16PRO_0000245816Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei187 – 1871PhosphothreonineBy similarity
    Modified residuei189 – 1891PhosphotyrosineBy similarity
    Modified residuei192 – 1921PhosphothreonineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8W4J2.
    PRIDEiQ8W4J2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8W4J2.

    Interactioni

    Protein-protein interaction databases

    BioGridi17295. 1 interaction.
    IntActiQ8W4J2. 1 interaction.
    STRINGi3702.AT5G19010.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8W4J2.
    SMRiQ8W4J2. Positions 22-399.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 316292Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi187 – 1893TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiQ8W4J2.
    OMAiMRKKKPI.
    PhylomeDBiQ8W4J2.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8W4J2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQPDHRKKSS VEVDFFTEYG EGSRYRIEEV IGKGSYGVVC SAYDTHTGEK    50
    VAIKKINDIF EHVSDATRIL REIKLLRLLR HPDIVEIKHI LLPPSRREFR 100
    DIYVVFELME SDLHQVIKAN DDLTPEHYQF FLYQLLRGLK YIHTANVFHR 150
    DLKPKNILAN ADCKLKICDF GLARVAFNDT PTAIFWTDYV ATRWYRAPEL 200
    CGSFFSKYTP AIDIWSIGCI FAELLTGKPL FPGKNVVHQL DLMTDMLGTP 250
    SAEAIGRVRN EKARRYLSSM RKKKPIPFSH KFPHTDPLAL RLLEKMLSFE 300
    PKDRPTAEEA LADVYFKGLA KVEREPSAQP VTKLEFEFER RRITKEDVRE 350
    LIYRESLEYH PKMLKEYLDG SEPTNFMYPS AVEHFKKQFA YLEEHYKNGT 400
    SHNPPERQQH ASLPRACVLY SDNNHPVAQQ SSAEVTDGLS KCSIRDERPR 450
    GADRNAQMPM SRIPINVPQT IQGAAVARPG KVVGSVLRYN NCGAATGVEA 500
    LEQQQRRMVR NPAAASQYPK RTQPCKSNRG DEDCATAAEG PSRLKPNTQY 550
    IPQKVSAAQD TAMSRWY 567
    Length:567
    Mass (Da):64,912
    Last modified:September 5, 2006 - v2
    Checksum:iF6D817F558EFCC33
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti397 – 3971K → R in AAL32607. (PubMed:14593172)Curated
    Sequence conflicti397 – 3971K → R in AAN15447. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC068809 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92639.1.
    AY062529 mRNA. Translation: AAL32607.1.
    BT000128 mRNA. Translation: AAN15447.1.
    RefSeqiNP_197402.1. NM_121906.3.
    UniGeneiAt.19792.
    At.20179.

    Genome annotation databases

    EnsemblPlantsiAT5G19010.1; AT5G19010.1; AT5G19010.
    GeneIDi832019.
    KEGGiath:AT5G19010.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC068809 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92639.1 .
    AY062529 mRNA. Translation: AAL32607.1 .
    BT000128 mRNA. Translation: AAN15447.1 .
    RefSeqi NP_197402.1. NM_121906.3.
    UniGenei At.19792.
    At.20179.

    3D structure databases

    ProteinModelPortali Q8W4J2.
    SMRi Q8W4J2. Positions 22-399.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 17295. 1 interaction.
    IntActi Q8W4J2. 1 interaction.
    STRINGi 3702.AT5G19010.1-P.

    Proteomic databases

    PaxDbi Q8W4J2.
    PRIDEi Q8W4J2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G19010.1 ; AT5G19010.1 ; AT5G19010 .
    GeneIDi 832019.
    KEGGi ath:AT5G19010.

    Organism-specific databases

    GeneFarmi 1568. 89.
    TAIRi AT5G19010.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi Q8W4J2.
    OMAi MRKKKPI.
    PhylomeDBi Q8W4J2.

    Enzyme and pathway databases

    BioCyci ARA:AT5G19010-MONOMER.

    Gene expression databases

    Genevestigatori Q8W4J2.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
      MAPK group
      Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. Cited for: GENE FAMILY.

    Entry informationi

    Entry nameiMPK16_ARATH
    AccessioniPrimary (citable) accession number: Q8W4J2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: September 5, 2006
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3