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Q8W4J2

- MPK16_ARATH

UniProt

Q8W4J2 - MPK16_ARATH

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Protein

Mitogen-activated protein kinase 16

Gene

MPK16

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541ATPPROSITE-ProRule annotation
Active sitei151 – 1511Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 399ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: TAIR

GO - Biological processi

  1. MAPK cascade Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G19010-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 16 (EC:2.7.11.24)
Short name:
AtMPK16
Short name:
MAP kinase 16
Gene namesi
Name:MPK16
Ordered Locus Names:At5g19010
ORF Names:T16G12.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G19010.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: TAIR
  2. vacuole Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 567567Mitogen-activated protein kinase 16PRO_0000245816Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei187 – 1871PhosphothreonineBy similarity
Modified residuei189 – 1891PhosphotyrosineBy similarity
Modified residuei192 – 1921PhosphothreonineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-187 and Tyr-189, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8W4J2.
PRIDEiQ8W4J2.

Expressioni

Gene expression databases

GenevestigatoriQ8W4J2.

Interactioni

Protein-protein interaction databases

BioGridi17295. 1 interaction.
IntActiQ8W4J2. 1 interaction.
STRINGi3702.AT5G19010.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ8W4J2.
SMRiQ8W4J2. Positions 22-399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 316292Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 1893TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ8W4J2.
OMAiMRKKKPI.
PhylomeDBiQ8W4J2.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8W4J2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQPDHRKKSS VEVDFFTEYG EGSRYRIEEV IGKGSYGVVC SAYDTHTGEK
60 70 80 90 100
VAIKKINDIF EHVSDATRIL REIKLLRLLR HPDIVEIKHI LLPPSRREFR
110 120 130 140 150
DIYVVFELME SDLHQVIKAN DDLTPEHYQF FLYQLLRGLK YIHTANVFHR
160 170 180 190 200
DLKPKNILAN ADCKLKICDF GLARVAFNDT PTAIFWTDYV ATRWYRAPEL
210 220 230 240 250
CGSFFSKYTP AIDIWSIGCI FAELLTGKPL FPGKNVVHQL DLMTDMLGTP
260 270 280 290 300
SAEAIGRVRN EKARRYLSSM RKKKPIPFSH KFPHTDPLAL RLLEKMLSFE
310 320 330 340 350
PKDRPTAEEA LADVYFKGLA KVEREPSAQP VTKLEFEFER RRITKEDVRE
360 370 380 390 400
LIYRESLEYH PKMLKEYLDG SEPTNFMYPS AVEHFKKQFA YLEEHYKNGT
410 420 430 440 450
SHNPPERQQH ASLPRACVLY SDNNHPVAQQ SSAEVTDGLS KCSIRDERPR
460 470 480 490 500
GADRNAQMPM SRIPINVPQT IQGAAVARPG KVVGSVLRYN NCGAATGVEA
510 520 530 540 550
LEQQQRRMVR NPAAASQYPK RTQPCKSNRG DEDCATAAEG PSRLKPNTQY
560
IPQKVSAAQD TAMSRWY
Length:567
Mass (Da):64,912
Last modified:September 5, 2006 - v2
Checksum:iF6D817F558EFCC33
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti397 – 3971K → R in AAL32607. (PubMed:14593172)Curated
Sequence conflicti397 – 3971K → R in AAN15447. (PubMed:14593172)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068809 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92639.1.
AY062529 mRNA. Translation: AAL32607.1.
BT000128 mRNA. Translation: AAN15447.1.
RefSeqiNP_197402.1. NM_121906.3.
UniGeneiAt.19792.
At.20179.

Genome annotation databases

EnsemblPlantsiAT5G19010.1; AT5G19010.1; AT5G19010.
GeneIDi832019.
KEGGiath:AT5G19010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068809 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92639.1 .
AY062529 mRNA. Translation: AAL32607.1 .
BT000128 mRNA. Translation: AAN15447.1 .
RefSeqi NP_197402.1. NM_121906.3.
UniGenei At.19792.
At.20179.

3D structure databases

ProteinModelPortali Q8W4J2.
SMRi Q8W4J2. Positions 22-399.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 17295. 1 interaction.
IntActi Q8W4J2. 1 interaction.
STRINGi 3702.AT5G19010.1-P.

Proteomic databases

PaxDbi Q8W4J2.
PRIDEi Q8W4J2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G19010.1 ; AT5G19010.1 ; AT5G19010 .
GeneIDi 832019.
KEGGi ath:AT5G19010.

Organism-specific databases

GeneFarmi 1568. 89.
TAIRi AT5G19010.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q8W4J2.
OMAi MRKKKPI.
PhylomeDBi Q8W4J2.

Enzyme and pathway databases

BioCyci ARA:AT5G19010-MONOMER.

Gene expression databases

Genevestigatori Q8W4J2.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. Cited for: GENE FAMILY.

Entry informationi

Entry nameiMPK16_ARATH
AccessioniPrimary (citable) accession number: Q8W4J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: September 5, 2006
Last modified: October 29, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3