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Protein

Calcium-dependent protein kinase 13

Gene

CPK13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei83ATPPROSITE-ProRule annotation1
Active sitei178Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi60 – 68ATPPROSITE-ProRule annotation9
Calcium bindingi368 – 3791PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi404 – 4152PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi440 – 4513PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi476 – 4874PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371571. HSF1-dependent transactivation.
R-ATH-442729. CREB phosphorylation through the activation of CaMKII.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 13 (EC:2.7.11.1)
Gene namesi
Name:CPK13
Ordered Locus Names:At3g51850
ORF Names:ATEM1.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G51850.
TAIRilocus:2074338. AT3G51850.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedSequence analysis
ChainiPRO_00003633352 – 528Calcium-dependent protein kinase 13Add BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Modified residuei43PhosphoserineCombined sources1
Modified residuei218PhosphoserineBy similarity1
Modified residuei484PhosphoserineBy similarity1
Modified residuei502PhosphoserineBy similarity1
Modified residuei522PhosphoserineCombined sources1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiQ8W4I7.
PRIDEiQ8W4I7.

PTM databases

iPTMnetiQ8W4I7.
SwissPalmiQ8W4I7.

Expressioni

Gene expression databases

ExpressionAtlasiQ8W4I7. baseline and differential.
GenevisibleiQ8W4I7. AT.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi9666. 4 interactors.
STRINGi3702.AT3G51850.1.

Structurei

3D structure databases

ProteinModelPortaliQ8W4I7.
SMRiQ8W4I7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 312Protein kinasePROSITE-ProRule annotationAdd BLAST259
Domaini355 – 390EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini391 – 426EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini427 – 462EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini463 – 498EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni318 – 348Autoinhibitory domainBy similarityAdd BLAST31

Domaini

There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (318-348) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ8W4I7.
KOiK13412.
OMAiTFEDHKN.
OrthoDBiEOG093606GJ.
PhylomeDBiQ8W4I7.

Family and domain databases

CDDicd00051. EFh. 1 hit.
InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8W4I7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNCCRSPAA VAREDVKSNY SGHDHARKDA AGGKKSAPIR VLSDVPKENI
60 70 80 90 100
EDRYLLDREL GRGEFGVTYL CIERSSRDLL ACKSISKRKL RTAVDIEDVK
110 120 130 140 150
REVAIMKHLP KSSSIVTLKE ACEDDNAVHL VMELCEGGEL FDRIVARGHY
160 170 180 190 200
TERAAAGVTK TIVEVVQLCH KHGVIHRDLK PENFLFANKK ENSPLKAIDF
210 220 230 240 250
GLSIFFKPGE KFSEIVGSPY YMAPEVLKRN YGPEIDIWSA GVILYILLCG
260 270 280 290 300
VPPFWAESEQ GVAQAILRGV IDFKREPWPN ISETAKNLVR QMLEPDPKRR
310 320 330 340 350
LTAKQVLEHP WIQNAKKAPN VPLGDVVKSR LKQFSVMNRF KRKALRVIAE
360 370 380 390 400
FLSTEEVEDI KVMFNKMDTD NDGIVSIEEL KAGLRDFSTQ LAESEVQMLI
410 420 430 440 450
EAVDTKGKGT LDYGEFVAVS LHLQKVANDE HLRKAFSYFD KDGNGYILPQ
460 470 480 490 500
ELCDALKEDG GDDCVDVAND IFQEVDTDKD GRISYEEFAA MMKTGTDWRK
510 520
ASRHYSRGRF NSLSIKLMKD GSLNLGNE
Length:528
Mass (Da):59,376
Last modified:February 10, 2009 - v2
Checksum:i00FEBE5C65B5CC60
GO

Sequence cautioni

The sequence AAA99794 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAC14412 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti350E → K in AAL32617 (PubMed:14593172).Curated1
Sequence conflicti350E → K in AAO29985 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54615 Genomic DNA. Translation: AAA99794.1. Sequence problems.
AF049236 Genomic DNA. Translation: AAC14412.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78852.1.
AY062539 mRNA. Translation: AAL32617.1.
BT003367 mRNA. Translation: AAO29985.1.
AK220740 mRNA. Translation: BAD93899.1.
PIRiT51156.
RefSeqiNP_190753.2. NM_115044.5.
UniGeneiAt.27306.
At.69291.

Genome annotation databases

EnsemblPlantsiAT3G51850.1; AT3G51850.1; AT3G51850.
GeneIDi824348.
GrameneiAT3G51850.1; AT3G51850.1; AT3G51850.
KEGGiath:AT3G51850.

Similar proteinsi

Entry informationi

Entry nameiCDPKD_ARATH
AccessioniPrimary (citable) accession number: Q8W4I7
Secondary accession number(s): Q570G8, Q96294
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: February 10, 2009
Last modified: November 22, 2017
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families