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Protein

V-type proton ATPase subunit B3

Gene

VHA-B3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

GO - Molecular functioni

GO - Biological processi

  • actin filament bundle assembly Source: TAIR
  • actin filament capping Source: TAIR
  • ATP hydrolysis coupled proton transport Source: InterPro
  • ATP metabolic process Source: InterPro
  • negative regulation of actin filament depolymerization Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciARA:AT1G20260-MONOMER.
ReactomeiR-ATH-1222556. ROS, RNS production in response to bacteria.
R-ATH-77387. Insulin receptor recycling.
R-ATH-917977. Transferrin endocytosis and recycling.

Protein family/group databases

TCDBi3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase subunit B3
Short name:
V-ATPase subunit B3
Alternative name(s):
Vacuolar H(+)-ATPase subunit B isoform 3
Vacuolar proton pump subunit B3
Gene namesi
Name:VHA-B3
Ordered Locus Names:At1g20260
ORF Names:F14O10.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G20260.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • Golgi apparatus Source: TAIR
  • proton-transporting V-type ATPase, V1 domain Source: InterPro
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 487486V-type proton ATPase subunit B3PRO_0000373817Add
BLAST

Proteomic databases

PaxDbiQ8W4E2.
PRIDEiQ8W4E2.

PTM databases

iPTMnetiQ8W4E2.

Expressioni

Gene expression databases

GenevisibleiQ8W4E2. AT.

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c'', d and e).

GO - Molecular functioni

  • actin filament binding Source: TAIR

Protein-protein interaction databases

BioGridi23853. 4 interactions.
IntActiQ8W4E2. 1 interaction.
STRINGi3702.AT1G20260.1.

Structurei

3D structure databases

ProteinModelPortaliQ8W4E2.
SMRiQ8W4E2. Positions 21-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Phylogenomic databases

eggNOGiKOG1351. Eukaryota.
COG1156. LUCA.
HOGENOMiHOG000165320.
InParanoidiQ8W4E2.
KOiK02147.
OMAiAEALREX.
OrthoDBiEOG093607DL.
PhylomeDBiQ8W4E2.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00310. ATP_synth_B_arch. 1 hit.
InterProiIPR020003. ATPase_a/bsu_AS.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR005723. ATPase_V1-cplx_bsu.
IPR027417. P-loop_NTPase.
IPR022879. V-ATPase_su_B/beta.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
PIRSFiPIRSF039114. V-ATPsynth_beta/V-ATPase_B. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01040. V-ATPase_V1_B. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8W4E2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVETSIDMEE GTLEIGMEYR TVSGVAGPLV ILDKVKGPKY QEIVNIRLGD
60 70 80 90 100
GSTRRGQVLE VDGEKAVVQV FEGTSGIDNK FTTVQFTGEV LKTPVSLDML
110 120 130 140 150
GRIFNGSGKP IDNGPPILPE AYLDISGSSI NPSERTYPEE MIQTGISTID
160 170 180 190 200
VMNSIARGQK IPLFSAAGLP HNEIAAQICR QAGLVKRLEK TENLIQEDHG
210 220 230 240 250
EDNFAIVFAA MGVNMETAQF FKRDFEENGS MERVTLFLNL ANDPTIERII
260 270 280 290 300
TPRIALTTAE YLAYECGKHV LVILTDMSSY ADALREVSAA REEVPGRRGY
310 320 330 340 350
PGYMYTDLAT IYERAGRIEG RKGSITQIPI LTMPNDDITH PTPDLTGYIT
360 370 380 390 400
EGQIYIDRQL HNRQIYPPIN VLPSLSRLMK SAIGEGMTRK DHSDVSNQLY
410 420 430 440 450
ANYAIGKDVQ AMKAVVGEEA LSSEDLLYLE FLDKFERKFV MQGAYDTRNI
460 470 480
FQSLDLAWTL LRIFPRELLH RIPAKTLDQF YSRDSTS
Length:487
Mass (Da):54,312
Last modified:March 1, 2002 - v1
Checksum:iB833A2CAE4D42CE8
GO

Sequence cautioni

The sequence AAF88162 differs from that shown. Reason: Frameshift at position 304. Curated
The sequence AAF88162 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC026234 Genomic DNA. Translation: AAF88162.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29955.1.
AY062616 mRNA. Translation: AAL32694.1.
BT000150 mRNA. Translation: AAN15469.1.
AK176408 mRNA. Translation: BAD44171.1.
AK176641 mRNA. Translation: BAD44404.1.
AK176750 mRNA. Translation: BAD44513.1.
AK176915 mRNA. Translation: BAD44678.1.
PIRiC86336.
RefSeqiNP_173451.5. NM_101877.5.
UniGeneiAt.24526.

Genome annotation databases

EnsemblPlantsiAT1G20260.1; AT1G20260.1; AT1G20260.
GeneIDi838614.
GrameneiAT1G20260.1; AT1G20260.1; AT1G20260.
KEGGiath:AT1G20260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC026234 Genomic DNA. Translation: AAF88162.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE29955.1.
AY062616 mRNA. Translation: AAL32694.1.
BT000150 mRNA. Translation: AAN15469.1.
AK176408 mRNA. Translation: BAD44171.1.
AK176641 mRNA. Translation: BAD44404.1.
AK176750 mRNA. Translation: BAD44513.1.
AK176915 mRNA. Translation: BAD44678.1.
PIRiC86336.
RefSeqiNP_173451.5. NM_101877.5.
UniGeneiAt.24526.

3D structure databases

ProteinModelPortaliQ8W4E2.
SMRiQ8W4E2. Positions 21-481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi23853. 4 interactions.
IntActiQ8W4E2. 1 interaction.
STRINGi3702.AT1G20260.1.

Protein family/group databases

TCDBi3.A.2.2.5. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

PTM databases

iPTMnetiQ8W4E2.

Proteomic databases

PaxDbiQ8W4E2.
PRIDEiQ8W4E2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G20260.1; AT1G20260.1; AT1G20260.
GeneIDi838614.
GrameneiAT1G20260.1; AT1G20260.1; AT1G20260.
KEGGiath:AT1G20260.

Organism-specific databases

TAIRiAT1G20260.

Phylogenomic databases

eggNOGiKOG1351. Eukaryota.
COG1156. LUCA.
HOGENOMiHOG000165320.
InParanoidiQ8W4E2.
KOiK02147.
OMAiAEALREX.
OrthoDBiEOG093607DL.
PhylomeDBiQ8W4E2.

Enzyme and pathway databases

BioCyciARA:AT1G20260-MONOMER.
ReactomeiR-ATH-1222556. ROS, RNS production in response to bacteria.
R-ATH-77387. Insulin receptor recycling.
R-ATH-917977. Transferrin endocytosis and recycling.

Miscellaneous databases

PROiQ8W4E2.

Gene expression databases

GenevisibleiQ8W4E2. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00310. ATP_synth_B_arch. 1 hit.
InterProiIPR020003. ATPase_a/bsu_AS.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR004100. ATPase_F1_a/bsu_N.
IPR005723. ATPase_V1-cplx_bsu.
IPR027417. P-loop_NTPase.
IPR022879. V-ATPase_su_B/beta.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
[Graphical view]
PIRSFiPIRSF039114. V-ATPsynth_beta/V-ATPase_B. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01040. V-ATPase_V1_B. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVATB3_ARATH
AccessioniPrimary (citable) accession number: Q8W4E2
Secondary accession number(s): Q9LN19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2002
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.