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Protein

V-type proton ATPase subunit B3

Gene

VHA-B3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

GO - Molecular functioni

  • actin filament binding Source: TAIR
  • ATP binding Source: InterPro
  • hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  • actin filament bundle assembly Source: TAIR
  • actin filament capping Source: TAIR
  • ATP hydrolysis coupled proton transport Source: InterPro
  • ATP metabolic process Source: InterPro
  • negative regulation of actin filament depolymerization Source: TAIR

Keywordsi

Molecular functionHydrolase
Biological processHydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciARA:AT1G20260-MONOMER
ReactomeiR-ATH-1222556 ROS, RNS production in phagocytes
R-ATH-77387 Insulin receptor recycling
R-ATH-917977 Transferrin endocytosis and recycling

Protein family/group databases

TCDBi3.A.2.2.5 the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase subunit B3
Short name:
V-ATPase subunit B3
Alternative name(s):
Vacuolar H(+)-ATPase subunit B isoform 3
Vacuolar proton pump subunit B3
Gene namesi
Name:VHA-B3
Ordered Locus Names:At1g20260
ORF Names:F14O10.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G20260
TAIRilocus:2012913 AT1G20260

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003738171 – 487V-type proton ATPase subunit B3Add BLAST487

Proteomic databases

PaxDbiQ8W4E2
PRIDEiQ8W4E2

PTM databases

iPTMnetiQ8W4E2

Expressioni

Gene expression databases

ExpressionAtlasiQ8W4E2 baseline and differential
GenevisibleiQ8W4E2 AT

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c'', d and e).

GO - Molecular functioni

  • actin filament binding Source: TAIR

Protein-protein interaction databases

BioGridi23853, 4 interactors
IntActiQ8W4E2, 1 interactor
STRINGi3702.AT1G20260.1

Structurei

3D structure databases

ProteinModelPortaliQ8W4E2
SMRiQ8W4E2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Phylogenomic databases

eggNOGiKOG1351 Eukaryota
COG1156 LUCA
HOGENOMiHOG000165320
InParanoidiQ8W4E2
KOiK02147
OMAiLMPFKKP
OrthoDBiEOG093607DL
PhylomeDBiQ8W4E2

Family and domain databases

HAMAPiMF_00310 ATP_synth_B_arch, 1 hit
InterProiView protein in InterPro
IPR020003 ATPase_a/bsu_AS
IPR004100 ATPase_F1/V1/A1_a/bsu_N
IPR000194 ATPase_F1/V1/A1_a/bsu_nucl-bd
IPR005723 ATPase_V1-cplx_bsu
IPR027417 P-loop_NTPase
IPR022879 V-ATPase_su_B/beta
PANTHERiPTHR43389 PTHR43389, 1 hit
PfamiView protein in Pfam
PF00006 ATP-synt_ab, 1 hit
PF02874 ATP-synt_ab_N, 1 hit
PIRSFiPIRSF039114 V-ATPsynth_beta/V-ATPase_B, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01040 V-ATPase_V1_B, 1 hit
PROSITEiView protein in PROSITE
PS00152 ATPASE_ALPHA_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

Q8W4E2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVETSIDMEE GTLEIGMEYR TVSGVAGPLV ILDKVKGPKY QEIVNIRLGD
60 70 80 90 100
GSTRRGQVLE VDGEKAVVQV FEGTSGIDNK FTTVQFTGEV LKTPVSLDML
110 120 130 140 150
GRIFNGSGKP IDNGPPILPE AYLDISGSSI NPSERTYPEE MIQTGISTID
160 170 180 190 200
VMNSIARGQK IPLFSAAGLP HNEIAAQICR QAGLVKRLEK TENLIQEDHG
210 220 230 240 250
EDNFAIVFAA MGVNMETAQF FKRDFEENGS MERVTLFLNL ANDPTIERII
260 270 280 290 300
TPRIALTTAE YLAYECGKHV LVILTDMSSY ADALREVSAA REEVPGRRGY
310 320 330 340 350
PGYMYTDLAT IYERAGRIEG RKGSITQIPI LTMPNDDITH PTPDLTGYIT
360 370 380 390 400
EGQIYIDRQL HNRQIYPPIN VLPSLSRLMK SAIGEGMTRK DHSDVSNQLY
410 420 430 440 450
ANYAIGKDVQ AMKAVVGEEA LSSEDLLYLE FLDKFERKFV MQGAYDTRNI
460 470 480
FQSLDLAWTL LRIFPRELLH RIPAKTLDQF YSRDSTS
Length:487
Mass (Da):54,312
Last modified:March 1, 2002 - v1
Checksum:iB833A2CAE4D42CE8
GO

Sequence cautioni

The sequence AAF88162 differs from that shown. Reason: Frameshift at position 304.Curated
The sequence AAF88162 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC026234 Genomic DNA Translation: AAF88162.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE29955.1
AY062616 mRNA Translation: AAL32694.1
BT000150 mRNA Translation: AAN15469.1
AK176408 mRNA Translation: BAD44171.1
AK176641 mRNA Translation: BAD44404.1
AK176750 mRNA Translation: BAD44513.1
AK176915 mRNA Translation: BAD44678.1
PIRiC86336
RefSeqiNP_173451.5, NM_101877.6
UniGeneiAt.24526

Genome annotation databases

EnsemblPlantsiAT1G20260.1; AT1G20260.1; AT1G20260
GeneIDi838614
GrameneiAT1G20260.1; AT1G20260.1; AT1G20260
KEGGiath:AT1G20260

Similar proteinsi

Entry informationi

Entry nameiVATB3_ARATH
AccessioniPrimary (citable) accession number: Q8W4E2
Secondary accession number(s): Q9LN19
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: March 1, 2002
Last modified: April 25, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health