Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8W4D8 (DDL_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
FHA domain-containing protein DDL
Alternative name(s):
Protein DAWDLE
Gene names
Name:DDL
Ordered Locus Names:At3g20550
ORF Names:K10D20.9
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. May facilitate DCL1 to access or recognize primary miRNAs. Binds RNA non-specifically. Ref.6

Subunit structure

Interacts with DCL1 (via N-terminus). Ref.6

Subcellular location

Nucleus Ref.4.

Tissue specificity

Expressed in roots, lateral roots, vascular strands of roots and leaves, vegetative meristems, pollen and developing seeds. Ref.4

Disruption phenotype

Delayed growth and reduced fertility. Defective roots, shoots and flowers. Reduced seed set. Reduced levels of primary miRNAs as well as mature miRNAs. Ref.4 Ref.6

Sequence similarities

Contains 1 FHA domain.

Sequence caution

The sequence BAB01163.1 differs from that shown. Reason: Erroneous gene model prediction.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DCL1Q9SP322EBI-2015534,EBI-632627

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314FHA domain-containing protein DDL
PRO_0000392079

Regions

Domain219 – 28264FHA
Compositional bias6 – 122117Arg-rich

Amino acid modifications

Modified residue1331Phosphoserine Ref.5 Ref.7 Ref.8

Secondary structure

................... 314
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8W4D8 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 42F3A7C9DFF14B68

FASTA31436,953
        10         20         30         40         50         60 
MAPSSRSPSP RTKRLRRARG EKEIGRSRER EDDGREREKR NSRERDRDIG RDRDRERKGE 

        70         80         90        100        110        120 
GERDREVGDK RRRSGREDTE KRRRTRTDDE RYSRGRHERS TSPSDRSHRS SRRSPERAIA 

       130        140        150        160        170        180 
SRHDEGSNAR GGSEEPNVEE DSVARMRAVE EALAAKKKEE PSFELSGKLA EETNRYRGIT 

       190        200        210        220        230        240 
LLFNEPPEAR KPSERWRLYV FKDGEPLNEP LCLHRQSCYL FGRERRIADI PTDHPSCSKQ 

       250        260        270        280        290        300 
HAVIQYREME KEKPDGMMGK QVKPYIMDLG STNKTYINES PIEPQRYYEL FEKDTIKFGN 

       310 
SSREYVLLHE NSAE 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"DAWDLE, a forkhead-associated domain gene, regulates multiple aspects of plant development."
Morris E.R., Chevalier D., Walker J.C.
Plant Physiol. 141:932-941(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[5]"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis."
de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H.
J. Proteome Res. 7:2458-2470(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Root.
[6]"The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis."
Yu B., Bi L., Zheng B., Ji L., Chevalier D., Agarwal M., Ramachandran V., Li W., Lagrange T., Walker J.C., Chen X.
Proc. Natl. Acad. Sci. U.S.A. 105:10073-10078(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH DCL1.
[7]"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
[8]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP000410 Genomic DNA. Translation: BAB01163.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76394.1.
AY062626 mRNA. Translation: AAL32704.1.
BT000005 mRNA. Translation: AAN15324.1.
RefSeqNP_188691.1. NM_112947.3.
UniGeneAt.27430.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3VPYX-ray1.70A180-310[»]
ProteinModelPortalQ8W4D8.
SMRQ8W4D8. Positions 180-314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid6933. 5 interactions.
DIPDIP-46412N.
IntActQ8W4D8. 5 interactions.
STRING3702.AT3G20550.1-P.

Proteomic databases

PaxDbQ8W4D8.
PRIDEQ8W4D8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G20550.1; AT3G20550.1; AT3G20550.
GeneID821601.
KEGGath:AT3G20550.

Organism-specific databases

TAIRAT3G20550.

Phylogenomic databases

eggNOGNOG271234.
HOGENOMHOG000264922.
InParanoidQ8W4D8.
KOK13108.
OMAERDRDIG.
PhylomeDBQ8W4D8.

Gene expression databases

GenevestigatorQ8W4D8.

Family and domain databases

Gene3D2.60.200.20. 1 hit.
InterProIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamPF00498. FHA. 1 hit.
[Graphical view]
SMARTSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMSSF49879. SSF49879. 1 hit.
PROSITEPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8W4D8.

Entry information

Entry nameDDL_ARATH
AccessionPrimary (citable) accession number: Q8W4D8
Secondary accession number(s): Q9LJU3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names