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Q8W4D8

- DDL_ARATH

UniProt

Q8W4D8 - DDL_ARATH

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Protein

FHA domain-containing protein DDL

Gene

DDL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. May facilitate DCL1 to access or recognize primary miRNAs. Binds RNA non-specifically.1 Publication

GO - Molecular functioni

  1. RNA binding Source: UniProtKB

GO - Biological processi

  1. cell proliferation Source: TAIR
  2. production of miRNAs involved in gene silencing by miRNA Source: TAIR
  3. regulation of developmental growth Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

RNA-mediated gene silencing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
FHA domain-containing protein DDL
Alternative name(s):
Protein DAWDLE
Gene namesi
Name:DDL
Ordered Locus Names:At3g20550
ORF Names:K10D20.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G20550.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. cytosol Source: TAIR
  3. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Delayed growth and reduced fertility. Defective roots, shoots and flowers. Reduced seed set. Reduced levels of primary miRNAs as well as mature miRNAs.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 314314FHA domain-containing protein DDLPRO_0000392079Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei133 – 1331Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8W4D8.
PRIDEiQ8W4D8.

Expressioni

Tissue specificityi

Expressed in roots, lateral roots, vascular strands of roots and leaves, vegetative meristems, pollen and developing seeds.1 Publication

Gene expression databases

GenevestigatoriQ8W4D8.

Interactioni

Subunit structurei

Interacts with DCL1 (via N-terminus).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
DCL1Q9SP322EBI-2015534,EBI-632627

Protein-protein interaction databases

BioGridi6933. 5 interactions.
DIPiDIP-46412N.
IntActiQ8W4D8. 5 interactions.
STRINGi3702.AT3G20550.1-P.

Structurei

Secondary structure

1
314
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi197 – 2026Combined sources
Beta strandi214 – 22310Combined sources
Turni225 – 2273Combined sources
Beta strandi229 – 2313Combined sources
Beta strandi242 – 25211Combined sources
Beta strandi258 – 26811Combined sources
Beta strandi275 – 2773Combined sources
Beta strandi295 – 2984Combined sources
Beta strandi302 – 3087Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VPYX-ray1.70A180-310[»]
ProteinModelPortaliQ8W4D8.
SMRiQ8W4D8. Positions 180-314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini219 – 28264FHAPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 122117Arg-richAdd
BLAST

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG271234.
HOGENOMiHOG000264922.
KOiK13108.
OMAiERDRDIG.
PhylomeDBiQ8W4D8.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8W4D8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPSSRSPSP RTKRLRRARG EKEIGRSRER EDDGREREKR NSRERDRDIG
60 70 80 90 100
RDRDRERKGE GERDREVGDK RRRSGREDTE KRRRTRTDDE RYSRGRHERS
110 120 130 140 150
TSPSDRSHRS SRRSPERAIA SRHDEGSNAR GGSEEPNVEE DSVARMRAVE
160 170 180 190 200
EALAAKKKEE PSFELSGKLA EETNRYRGIT LLFNEPPEAR KPSERWRLYV
210 220 230 240 250
FKDGEPLNEP LCLHRQSCYL FGRERRIADI PTDHPSCSKQ HAVIQYREME
260 270 280 290 300
KEKPDGMMGK QVKPYIMDLG STNKTYINES PIEPQRYYEL FEKDTIKFGN
310
SSREYVLLHE NSAE
Length:314
Mass (Da):36,953
Last modified:March 1, 2002 - v1
Checksum:i42F3A7C9DFF14B68
GO

Sequence cautioni

The sequence BAB01163.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000410 Genomic DNA. Translation: BAB01163.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76394.1.
AY062626 mRNA. Translation: AAL32704.1.
BT000005 mRNA. Translation: AAN15324.1.
RefSeqiNP_188691.1. NM_112947.3.
UniGeneiAt.27430.

Genome annotation databases

EnsemblPlantsiAT3G20550.1; AT3G20550.1; AT3G20550.
GeneIDi821601.
KEGGiath:AT3G20550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000410 Genomic DNA. Translation: BAB01163.1 . Sequence problems.
CP002686 Genomic DNA. Translation: AEE76394.1 .
AY062626 mRNA. Translation: AAL32704.1 .
BT000005 mRNA. Translation: AAN15324.1 .
RefSeqi NP_188691.1. NM_112947.3.
UniGenei At.27430.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3VPY X-ray 1.70 A 180-310 [» ]
ProteinModelPortali Q8W4D8.
SMRi Q8W4D8. Positions 180-314.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 6933. 5 interactions.
DIPi DIP-46412N.
IntActi Q8W4D8. 5 interactions.
STRINGi 3702.AT3G20550.1-P.

Proteomic databases

PaxDbi Q8W4D8.
PRIDEi Q8W4D8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G20550.1 ; AT3G20550.1 ; AT3G20550 .
GeneIDi 821601.
KEGGi ath:AT3G20550.

Organism-specific databases

TAIRi AT3G20550.

Phylogenomic databases

eggNOGi NOG271234.
HOGENOMi HOG000264922.
KOi K13108.
OMAi ERDRDIG.
PhylomeDBi Q8W4D8.

Miscellaneous databases

PROi Q8W4D8.

Gene expression databases

Genevestigatori Q8W4D8.

Family and domain databases

Gene3Di 2.60.200.20. 1 hit.
InterProi IPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view ]
Pfami PF00498. FHA. 1 hit.
[Graphical view ]
SMARTi SM00240. FHA. 1 hit.
[Graphical view ]
SUPFAMi SSF49879. SSF49879. 1 hit.
PROSITEi PS50006. FHA_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
    Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
    DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "DAWDLE, a forkhead-associated domain gene, regulates multiple aspects of plant development."
    Morris E.R., Chevalier D., Walker J.C.
    Plant Physiol. 141:932-941(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Root.
  6. "The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis."
    Yu B., Bi L., Zheng B., Ji L., Chevalier D., Agarwal M., Ramachandran V., Li W., Lagrange T., Walker J.C., Chen X.
    Proc. Natl. Acad. Sci. U.S.A. 105:10073-10078(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH DCL1.
  7. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDDL_ARATH
AccessioniPrimary (citable) accession number: Q8W4D8
Secondary accession number(s): Q9LJU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: March 1, 2002
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3