FHA domain-containing protein DDL - Arabidopsis thaliana (Mouse-ear cress)

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Q8W4D8 (DDL_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 66. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
FHA domain-containing protein DDL

Alternative name(s):
Protein DAWDLE

Gene names

Name:	DDL
Ordered Locus Names:	At3g20550
ORF Names:	K10D20.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. May facilitate DCL1 to access or recognize primary miRNAs. Binds RNA non-specifically. Ref.6
Subunit structure	Interacts with DCL1 (via N-terminus). Ref.6
Subcellular location	Nucleus Ref.4.
Tissue specificity	Expressed in roots, lateral roots, vascular strands of roots and leaves, vegetative meristems, pollen and developing seeds. Ref.4
Disruption phenotype	Delayed growth and reduced fertility. Defective roots, shoots and flowers. Reduced seed set. Reduced levels of primary miRNAs as well as mature miRNAs. Ref.4 Ref.6
Sequence similarities	Contains 1 FHA domain.
Sequence caution	The sequence BAB01163.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	RNA-mediated gene silencing
Cellular component	Nucleus
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Inferred from mutant phenotype Ref.4. Source: TAIR production of miRNAs involved in gene silencing by miRNA Inferred from direct assay Ref.6. Source: TAIR regulation of developmental growth Inferred from mutant phenotype Ref.4. Source: UniProtKB
Cellular_component	chloroplast Inferred from direct assay PubMed 15028209. Source: TAIR cytosol Inferred from direct assay Ref.5. Source: TAIR nucleus Inferred from direct assay Ref.4 Ref.5. Source: TAIR
Molecular_function	RNA binding Inferred from mutant phenotype Ref.6. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DCL1	Q9SP32	2	EBI-2015534,EBI-632627

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 314

314

FHA domain-containing protein DDL

PRO_0000392079

Regions

Domain

219 – 282

FHA

Compositional bias

6 – 122

117

Arg-rich

Amino acid modifications

Modified residue

133

Phosphoserine Ref.5 Ref.8

Modified residue

301

Phosphoserine Ref.7

Secondary structure

314

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q8W4D8 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 42F3A7C9DFF14B68
Show »

FASTA

314

36,953

        10         20         30         40         50         60 
MAPSSRSPSP RTKRLRRARG EKEIGRSRER EDDGREREKR NSRERDRDIG RDRDRERKGE 

        70         80         90        100        110        120 
GERDREVGDK RRRSGREDTE KRRRTRTDDE RYSRGRHERS TSPSDRSHRS SRRSPERAIA 

       130        140        150        160        170        180 
SRHDEGSNAR GGSEEPNVEE DSVARMRAVE EALAAKKKEE PSFELSGKLA EETNRYRGIT 

       190        200        210        220        230        240 
LLFNEPPEAR KPSERWRLYV FKDGEPLNEP LCLHRQSCYL FGRERRIADI PTDHPSCSKQ 

       250        260        270        280        290        300 
HAVIQYREME KEKPDGMMGK QVKPYIMDLG STNKTYINES PIEPQRYYEL FEKDTIKFGN 

       310 
SSREYVLLHE NSAE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"DAWDLE, a forkhead-associated domain gene, regulates multiple aspects of plant development." Morris E.R., Chevalier D., Walker J.C. Plant Physiol. 141:932-941(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[5]	"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis." de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H. J. Proteome Res. 7:2458-2470(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, MASS SPECTROMETRY. Tissue: Root.
[6]	"The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis." Yu B., Bi L., Zheng B., Ji L., Chevalier D., Agarwal M., Ramachandran V., Li W., Lagrange T., Walker J.C., Chen X. Proc. Natl. Acad. Sci. U.S.A. 105:10073-10078(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH DCL1.
[7]	"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana." Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C. J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-301, MASS SPECTROMETRY. Strain: cv. Columbia.
[8]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP000410 Genomic DNA. Translation: BAB01163.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76394.1.
AY062626 mRNA. Translation: AAL32704.1.
BT000005 mRNA. Translation: AAN15324.1.

IPI

IPI00526203.

RefSeq

NP_188691.1. NM_112947.3.

UniGene

At.27430.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3VPY	X-ray	1.70	A	180-314	[»]

ProteinModelPortal

Q8W4D8.

SMR

Q8W4D8. Positions 182-307.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46412N.

IntAct

Q8W4D8. 5 interactions.

STRING

3702.AT3G20550.1-P.

Proteomic databases

PaxDb

Q8W4D8.

PRIDE

Q8W4D8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT3G20550.1; AT3G20550.1; AT3G20550.

GeneID

821601.

KEGG

ath:AT3G20550.

Organism-specific databases

TAIR

At3g20550.

Phylogenomic databases

eggNOG

NOG271234.

HOGENOM

HOG000264922.

InParanoid

Q8W4D8.

K13108.

OMA

YINESPI.

PhylomeDB

Q8W4D8.

ProtClustDB

CLSN2915617.

Gene expression databases

Genevestigator

Q8W4D8.

Family and domain databases

Gene3D

2.60.200.20. 1 hit.

InterPro

IPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]

Pfam

PF00498. FHA. 1 hit.
[Graphical view]

SMART

SM00240. FHA. 1 hit.
[Graphical view]

SUPFAM

SSF49879. SMAD_FHA. 1 hit.

PROSITE

PS50006. FHA_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DDL_ARATH

Accession

Primary (citable) accession number: Q8W4D8
Secondary accession number(s): Q9LJU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 2, 2010
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents