Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8W4D8

- DDL_ARATH

UniProt

Q8W4D8 - DDL_ARATH

Protein

FHA domain-containing protein DDL

Gene

DDL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. May facilitate DCL1 to access or recognize primary miRNAs. Binds RNA non-specifically.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA binding Source: UniProtKB

    GO - Biological processi

    1. cell proliferation Source: TAIR
    2. production of miRNAs involved in gene silencing by miRNA Source: TAIR
    3. regulation of developmental growth Source: UniProtKB

    Keywords - Biological processi

    RNA-mediated gene silencing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    FHA domain-containing protein DDL
    Alternative name(s):
    Protein DAWDLE
    Gene namesi
    Name:DDL
    Ordered Locus Names:At3g20550
    ORF Names:K10D20.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G20550.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. cytosol Source: TAIR
    3. nucleus Source: TAIR

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Delayed growth and reduced fertility. Defective roots, shoots and flowers. Reduced seed set. Reduced levels of primary miRNAs as well as mature miRNAs.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314FHA domain-containing protein DDLPRO_0000392079Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei133 – 1331Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ8W4D8.
    PRIDEiQ8W4D8.

    Expressioni

    Tissue specificityi

    Expressed in roots, lateral roots, vascular strands of roots and leaves, vegetative meristems, pollen and developing seeds.1 Publication

    Gene expression databases

    GenevestigatoriQ8W4D8.

    Interactioni

    Subunit structurei

    Interacts with DCL1 (via N-terminus).1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DCL1Q9SP322EBI-2015534,EBI-632627

    Protein-protein interaction databases

    BioGridi6933. 5 interactions.
    DIPiDIP-46412N.
    IntActiQ8W4D8. 5 interactions.
    STRINGi3702.AT3G20550.1-P.

    Structurei

    Secondary structure

    1
    314
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi197 – 2026
    Beta strandi214 – 22310
    Turni225 – 2273
    Beta strandi229 – 2313
    Beta strandi242 – 25211
    Beta strandi258 – 26811
    Beta strandi275 – 2773
    Beta strandi295 – 2984
    Beta strandi302 – 3087

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VPYX-ray1.70A180-310[»]
    ProteinModelPortaliQ8W4D8.
    SMRiQ8W4D8. Positions 180-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini219 – 28264FHAPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi6 – 122117Arg-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FHA domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG271234.
    HOGENOMiHOG000264922.
    InParanoidiQ8W4D8.
    KOiK13108.
    OMAiERDRDIG.
    PhylomeDBiQ8W4D8.

    Family and domain databases

    Gene3Di2.60.200.20. 1 hit.
    InterProiIPR000253. FHA_dom.
    IPR008984. SMAD_FHA_domain.
    [Graphical view]
    PfamiPF00498. FHA. 1 hit.
    [Graphical view]
    SMARTiSM00240. FHA. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    PROSITEiPS50006. FHA_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8W4D8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPSSRSPSP RTKRLRRARG EKEIGRSRER EDDGREREKR NSRERDRDIG    50
    RDRDRERKGE GERDREVGDK RRRSGREDTE KRRRTRTDDE RYSRGRHERS 100
    TSPSDRSHRS SRRSPERAIA SRHDEGSNAR GGSEEPNVEE DSVARMRAVE 150
    EALAAKKKEE PSFELSGKLA EETNRYRGIT LLFNEPPEAR KPSERWRLYV 200
    FKDGEPLNEP LCLHRQSCYL FGRERRIADI PTDHPSCSKQ HAVIQYREME 250
    KEKPDGMMGK QVKPYIMDLG STNKTYINES PIEPQRYYEL FEKDTIKFGN 300
    SSREYVLLHE NSAE 314
    Length:314
    Mass (Da):36,953
    Last modified:March 1, 2002 - v1
    Checksum:i42F3A7C9DFF14B68
    GO

    Sequence cautioni

    The sequence BAB01163.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000410 Genomic DNA. Translation: BAB01163.1. Sequence problems.
    CP002686 Genomic DNA. Translation: AEE76394.1.
    AY062626 mRNA. Translation: AAL32704.1.
    BT000005 mRNA. Translation: AAN15324.1.
    RefSeqiNP_188691.1. NM_112947.3.
    UniGeneiAt.27430.

    Genome annotation databases

    EnsemblPlantsiAT3G20550.1; AT3G20550.1; AT3G20550.
    GeneIDi821601.
    KEGGiath:AT3G20550.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP000410 Genomic DNA. Translation: BAB01163.1 . Sequence problems.
    CP002686 Genomic DNA. Translation: AEE76394.1 .
    AY062626 mRNA. Translation: AAL32704.1 .
    BT000005 mRNA. Translation: AAN15324.1 .
    RefSeqi NP_188691.1. NM_112947.3.
    UniGenei At.27430.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VPY X-ray 1.70 A 180-310 [» ]
    ProteinModelPortali Q8W4D8.
    SMRi Q8W4D8. Positions 180-314.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 6933. 5 interactions.
    DIPi DIP-46412N.
    IntActi Q8W4D8. 5 interactions.
    STRINGi 3702.AT3G20550.1-P.

    Proteomic databases

    PaxDbi Q8W4D8.
    PRIDEi Q8W4D8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G20550.1 ; AT3G20550.1 ; AT3G20550 .
    GeneIDi 821601.
    KEGGi ath:AT3G20550.

    Organism-specific databases

    TAIRi AT3G20550.

    Phylogenomic databases

    eggNOGi NOG271234.
    HOGENOMi HOG000264922.
    InParanoidi Q8W4D8.
    KOi K13108.
    OMAi ERDRDIG.
    PhylomeDBi Q8W4D8.

    Miscellaneous databases

    PROi Q8W4D8.

    Gene expression databases

    Genevestigatori Q8W4D8.

    Family and domain databases

    Gene3Di 2.60.200.20. 1 hit.
    InterProi IPR000253. FHA_dom.
    IPR008984. SMAD_FHA_domain.
    [Graphical view ]
    Pfami PF00498. FHA. 1 hit.
    [Graphical view ]
    SMARTi SM00240. FHA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    PROSITEi PS50006. FHA_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
      Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
      DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "DAWDLE, a forkhead-associated domain gene, regulates multiple aspects of plant development."
      Morris E.R., Chevalier D., Walker J.C.
      Plant Physiol. 141:932-941(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Root.
    6. "The FHA domain proteins DAWDLE in Arabidopsis and SNIP1 in humans act in small RNA biogenesis."
      Yu B., Bi L., Zheng B., Ji L., Chevalier D., Agarwal M., Ramachandran V., Li W., Lagrange T., Walker J.C., Chen X.
      Proc. Natl. Acad. Sci. U.S.A. 105:10073-10078(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH DCL1.
    7. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
      Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
      J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    8. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-133, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiDDL_ARATH
    AccessioniPrimary (citable) accession number: Q8W4D8
    Secondary accession number(s): Q9LJU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 2, 2010
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3