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Protein

FHA domain-containing protein DDL

Gene

DDL

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. May facilitate DCL1 to access or recognize primary miRNAs. Binds RNA non-specifically.1 Publication

GO - Molecular functioni

  • mRNA binding Source: GO_Central
  • RNA binding Source: UniProtKB

GO - Biological processi

  • cell proliferation Source: TAIR
  • production of miRNAs involved in gene silencing by miRNA Source: TAIR
  • regulation of developmental growth Source: UniProtKB

Keywordsi

Molecular functionRNA-binding
Biological processRNA-mediated gene silencing

Names & Taxonomyi

Protein namesi
Recommended name:
FHA domain-containing protein DDL
Alternative name(s):
Protein DAWDLE
Gene namesi
Name:DDL
Ordered Locus Names:At3g20550
ORF Names:K10D20.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G20550
TAIRilocus:2085740 AT3G20550

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Delayed growth and reduced fertility. Defective roots, shoots and flowers. Reduced seed set. Reduced levels of primary miRNAs as well as mature miRNAs.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi223R → A: Loss of interaction with DCL1. 1 Publication1
Mutagenesisi238S → A: Loss of interaction with DCL1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003920791 – 314FHA domain-containing protein DDLAdd BLAST314

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei133PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8W4D8

PTM databases

iPTMnetiQ8W4D8

Expressioni

Tissue specificityi

Expressed in roots, lateral roots, vascular strands of roots and leaves, vegetative meristems, pollen and developing seeds.1 Publication

Gene expression databases

ExpressionAtlasiQ8W4D8 baseline and differential
GenevisibleiQ8W4D8 AT

Interactioni

Subunit structurei

Interacts with DCL1 (via N-terminus).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DCL1Q9SP322EBI-2015534,EBI-632627

Protein-protein interaction databases

BioGridi6933, 5 interactors
DIPiDIP-46412N
IntActiQ8W4D8, 12 interactors
STRINGi3702.AT3G20550.1

Structurei

Secondary structure

1314
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi197 – 202Combined sources6
Beta strandi214 – 223Combined sources10
Turni225 – 227Combined sources3
Beta strandi229 – 231Combined sources3
Beta strandi242 – 252Combined sources11
Beta strandi258 – 268Combined sources11
Beta strandi275 – 277Combined sources3
Beta strandi295 – 298Combined sources4
Beta strandi302 – 308Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3VPYX-ray1.70A180-310[»]
ProteinModelPortaliQ8W4D8
SMRiQ8W4D8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini219 – 282FHAPROSITE-ProRule annotationAdd BLAST64

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 122Arg-richAdd BLAST117

Phylogenomic databases

eggNOGiKOG1882 Eukaryota
ENOG4111F4H LUCA
HOGENOMiHOG000264922
KOiK13108
OMAiLKYHEPA
OrthoDBiEOG09360LH1
PhylomeDBiQ8W4D8

Family and domain databases

CDDicd00060 FHA, 1 hit
InterProiView protein in InterPro
IPR000253 FHA_dom
IPR008984 SMAD_FHA_dom_sf
PfamiView protein in Pfam
PF00498 FHA, 1 hit
SMARTiView protein in SMART
SM00240 FHA, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
PROSITEiView protein in PROSITE
PS50006 FHA_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q8W4D8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSSRSPSP RTKRLRRARG EKEIGRSRER EDDGREREKR NSRERDRDIG
60 70 80 90 100
RDRDRERKGE GERDREVGDK RRRSGREDTE KRRRTRTDDE RYSRGRHERS
110 120 130 140 150
TSPSDRSHRS SRRSPERAIA SRHDEGSNAR GGSEEPNVEE DSVARMRAVE
160 170 180 190 200
EALAAKKKEE PSFELSGKLA EETNRYRGIT LLFNEPPEAR KPSERWRLYV
210 220 230 240 250
FKDGEPLNEP LCLHRQSCYL FGRERRIADI PTDHPSCSKQ HAVIQYREME
260 270 280 290 300
KEKPDGMMGK QVKPYIMDLG STNKTYINES PIEPQRYYEL FEKDTIKFGN
310
SSREYVLLHE NSAE
Length:314
Mass (Da):36,953
Last modified:March 1, 2002 - v1
Checksum:i42F3A7C9DFF14B68
GO

Sequence cautioni

The sequence BAB01163 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP000410 Genomic DNA Translation: BAB01163.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE76394.1
AY062626 mRNA Translation: AAL32704.1
BT000005 mRNA Translation: AAN15324.1
RefSeqiNP_188691.1, NM_112947.4
UniGeneiAt.27430

Genome annotation databases

EnsemblPlantsiAT3G20550.1; AT3G20550.1; AT3G20550
GeneIDi821601
GrameneiAT3G20550.1; AT3G20550.1; AT3G20550
KEGGiath:AT3G20550

Similar proteinsi

Entry informationi

Entry nameiDDL_ARATH
AccessioniPrimary (citable) accession number: Q8W4D8
Secondary accession number(s): Q9LJU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: March 1, 2002
Last modified: April 25, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health