Q8W491 (U73B3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glycosyltransferase 73B3
EC=2.4.1.-

Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT73B3
EC=2.4.1.91

Gene names

Name:	UGT73B3
Ordered Locus Names:	At4g34131
ORF Names:	F28A23.120

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Possesses quercetin 3-O-glucosyltransferase activity in vitro. Also active in vitro on benzoates and benzoate derivatives. Involved in stress or defense responses. Ref.5 Ref.6 Ref.7
Catalytic activity	UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.
Tissue specificity	Expressed in roots and flowers. Ref.7
Induction	Induced by pathogen infection, by H2O2 and by salicylic acid. Ref.7
Disruption phenotype	Decreased resistance to avirulent strains of P.syringae. Ref.7
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW response to other organism Inferred from expression pattern Ref.7. Source: TAIR
Molecular_function	flavonol 3-O-glucosyltransferase activity Inferred from electronic annotation. Source: EC quercetin 3-O-glucosyltransferase activity Inferred from direct assay Ref.6. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	UDP-glycosyltransferase 73B3		PRO_0000403936
Regions
	Region	355 – 357	3	UDP-glucose binding By similarity
	Region	372 – 380	9	UDP-glucose binding By similarity
	Region	394 – 397	4	UDP-glucose binding By similarity
Sites
	Binding site	297	1	UDP-glucose By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8W491 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: FAE0484361DD072A	FASTA	481	54,591
	10 20 30 40 50 60 MSSDPHRKLH VVFFPFMAYG HMIPTLDMAK LFSSRGAKST ILTTPLNSKI FQKPIERFKN 70 80 90 100 110 120 LNPSFEIDIQ IFDFPCVDLG LPEGCENVDF FTSNNNDDRQ YLTLKFFKST RFFKDQLEKL 130 140 150 160 170 180 LETTRPDCLI ADMFFPWATE AAEKFNVPRL VFHGTGYFSL CSEYCIRVHN PQNIVASRYE 190 200 210 220 230 240 PFVIPDLPGN IVITQEQIAD RDEESEMGKF MIEVKESDVK SSGVIVNSFY ELEPDYADFY 250 260 270 280 290 300 KSVVLKRAWH IGPLSVYNRG FEEKAERGKK ASINEVECLK WLDSKKPDSV IYISFGSVAC 310 320 330 340 350 360 FKNEQLFEIA AGLETSGANF IWVVRKNIGI EKEEWLPEGF EERVKGKGMI IRGWAPQVLI 370 380 390 400 410 420 LDHQATCGFV THCGWNSLLE GVAAGLPMVT WPVAAEQFYN EKLVTQVLRT GVSVGAKKNV 430 440 450 460 470 480 RTTGDFISRE KVVKAVREVL VGEEADERRE RAKKLAEMAK AAVEGGSSFN DLNSFIEEFT S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana." Li Y., Baldauf S., Lim E.K., Bowles D.J. J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
[5]	"The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates." Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J. J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides." Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J. Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Pathogen-responsive expression of glycosyltransferase genes UGT73B3 and UGT73B5 is necessary for resistance to Pseudomonas syringae pv tomato in Arabidopsis." Langlois-Meurinne M., Gachon C.M., Saindrenan P. Plant Physiol. 139:1890-1901(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INDUCTION BY PATHOGEN AND SALICYLIC ACID.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL161584 Genomic DNA. No translation available. CP002687 Genomic DNA. Translation: AEE86327.1. AY062753 mRNA. Translation: AAL32831.1. AY114680 mRNA. Translation: AAM47999.1.
IPI	IPI00525673.
RefSeq	NP_567953.1. NM_119574.3.
UniGene	At.27243. At.68482.
3D structure databases
ProteinModelPortal	Q8W491.
SMR	Q8W491. Positions 9-481.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT4G34131.1-P.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PaxDb	Q8W491.
PRIDE	Q8W491.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G34131.1; AT4G34131.1; AT4G34131.
GeneID	829559.
KEGG	ath:AT4G34131.
Organism-specific databases
TAIR	At4g34131.
Phylogenomic databases
eggNOG	NOG263906.
HOGENOM	HOG000237565.
InParanoid	Q8W491.
OMA	ETSGANF.
PhylomeDB	Q8W491.
ProtClustDB	PLN03007.
Gene expression databases
Genevestigator	Q8W491.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

U73B3_ARATH

Accession

Primary (citable) accession number: Q8W491

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program