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Q8W491 (U73B3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glycosyltransferase 73B3

EC=2.4.1.-
Alternative name(s):
Flavonol 3-O-glucosyltransferase UGT73B3
EC=2.4.1.91
Gene names
Name:UGT73B3
Ordered Locus Names:At4g34131
ORF Names:F28A23.120
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Possesses quercetin 3-O-glucosyltransferase activity in vitro. Also active in vitro on benzoates and benzoate derivatives. Involved in stress or defense responses. Ref.5 Ref.6 Ref.7

Catalytic activity

UDP-glucose + a flavonol = UDP + a flavonol 3-O-D-glucoside.

Tissue specificity

Expressed in roots and flowers. Ref.7

Induction

Induced by pathogen infection, by H2O2 and by salicylic acid. Ref.7

Disruption phenotype

Decreased resistance to avirulent strains of P.syringae. Ref.7

Sequence similarities

Belongs to the UDP-glycosyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 481481UDP-glycosyltransferase 73B3
PRO_0000403936

Regions

Region355 – 3573UDP-glucose binding By similarity
Region372 – 3809UDP-glucose binding By similarity
Region394 – 3974UDP-glucose binding By similarity

Sites

Binding site2971UDP-glucose By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8W491 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: FAE0484361DD072A

FASTA48154,591
        10         20         30         40         50         60 
MSSDPHRKLH VVFFPFMAYG HMIPTLDMAK LFSSRGAKST ILTTPLNSKI FQKPIERFKN 

        70         80         90        100        110        120 
LNPSFEIDIQ IFDFPCVDLG LPEGCENVDF FTSNNNDDRQ YLTLKFFKST RFFKDQLEKL 

       130        140        150        160        170        180 
LETTRPDCLI ADMFFPWATE AAEKFNVPRL VFHGTGYFSL CSEYCIRVHN PQNIVASRYE 

       190        200        210        220        230        240 
PFVIPDLPGN IVITQEQIAD RDEESEMGKF MIEVKESDVK SSGVIVNSFY ELEPDYADFY 

       250        260        270        280        290        300 
KSVVLKRAWH IGPLSVYNRG FEEKAERGKK ASINEVECLK WLDSKKPDSV IYISFGSVAC 

       310        320        330        340        350        360 
FKNEQLFEIA AGLETSGANF IWVVRKNIGI EKEEWLPEGF EERVKGKGMI IRGWAPQVLI 

       370        380        390        400        410        420 
LDHQATCGFV THCGWNSLLE GVAAGLPMVT WPVAAEQFYN EKLVTQVLRT GVSVGAKKNV 

       430        440        450        460        470        480 
RTTGDFISRE KVVKAVREVL VGEEADERRE RAKKLAEMAK AAVEGGSSFN DLNSFIEEFT 


S 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Phylogenetic analysis of the UDP-glycosyltransferase multigene family of Arabidopsis thaliana."
Li Y., Baldauf S., Lim E.K., Bowles D.J.
J. Biol. Chem. 276:4338-4343(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[5]"The activity of Arabidopsis glycosyltransferases toward salicylic acid, 4-hydroxybenzoic acid, and other benzoates."
Lim E.K., Doucet C.J., Li Y., Elias L., Worrall D., Spencer S.P., Ross J., Bowles D.J.
J. Biol. Chem. 277:586-592(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides."
Lim E.K., Ashford D.A., Hou B., Jackson R.G., Bowles D.J.
Biotechnol. Bioeng. 87:623-631(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Pathogen-responsive expression of glycosyltransferase genes UGT73B3 and UGT73B5 is necessary for resistance to Pseudomonas syringae pv tomato in Arabidopsis."
Langlois-Meurinne M., Gachon C.M., Saindrenan P.
Plant Physiol. 139:1890-1901(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INDUCTION BY PATHOGEN AND SALICYLIC ACID.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL161584 Genomic DNA. No translation available.
CP002687 Genomic DNA. Translation: AEE86327.1.
AY062753 mRNA. Translation: AAL32831.1.
AY114680 mRNA. Translation: AAM47999.1.
RefSeqNP_567953.1. NM_119574.3.
UniGeneAt.27243.
At.68482.

3D structure databases

ProteinModelPortalQ8W491.
SMRQ8W491. Positions 9-481.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT4G34131.1-P.

Protein family/group databases

CAZyGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbQ8W491.
PRIDEQ8W491.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT4G34131.1; AT4G34131.1; AT4G34131.
GeneID829559.
KEGGath:AT4G34131.

Organism-specific databases

TAIRAT4G34131.

Phylogenomic databases

eggNOGNOG263906.
HOGENOMHOG000237565.
InParanoidQ8W491.
OMAETSGANF.
PhylomeDBQ8W491.

Gene expression databases

GenevestigatorQ8W491.

Family and domain databases

InterProIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERPTHR11926. PTHR11926. 1 hit.
PfamPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameU73B3_ARATH
AccessionPrimary (citable) accession number: Q8W491
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names